SRR1780249_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780249_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278635
Sequences flagged as poor quality	0
Sequence length	50
%GC	60

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGT	13956	5.008703142103469	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC	4129	1.4818669585658657	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCG	3711	1.3318499111741167	No Hit
CACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTG	2486	0.8922066502772444	Illumina PCR Primer Index 4 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC	847	0.3039819118201231	No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTT	296	0.10623216753099934	RNA PCR Primer, Index 27 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTTATA	20	7.847308E-4	43.998745	3
CTTTATA	130	0.0	37.229706	2
TCTTCTG	320	0.0	37.12394	44
GGCCCAA	30	1.289209E-4	36.711742	1
ATAGCAA	30	1.2987838E-4	36.66562	3
GTCTTCT	335	0.0	35.461674	43
CGTCTTC	345	0.0	34.4338	42
ATGCCGT	1965	0.0	33.586826	44
TCTTTAT	150	0.0	32.30633	1
GTCATCT	35	3.2120963E-4	31.427673	11
GCACACT	35	3.2120963E-4	31.427673	36
TTGCAGG	35	3.2120963E-4	31.427673	30
CCGTCTT	385	0.0	30.856262	41
TTTATAC	180	0.0	30.554682	3
TTATGTC	30	0.005738326	29.332497	44
ATAGTGC	30	0.005738326	29.332497	26
TGTAAGC	30	0.005738326	29.332497	2
TCTATTA	30	0.005738326	29.332497	2
TATGCCG	2315	0.0	28.603935	43
GCCGTCT	430	0.0	27.627117	40