FastQCFastQC Report
Wed 25 May 2016
SRR1780248_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780248_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120276
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACAA150.002220049570.0000133
TGTGTCC150.002220049570.000017
TAGCTAC252.3824078E-455.99999654
GATACAG200.006936984652.540
CGTGAAA200.006936984652.517
AGGAACC200.006936984652.525
GCACAAG200.006936984652.534
GGAACCA200.006936984652.526
CAAGAGT200.006936984652.537
GCTACAG200.006936984652.53
TACAGTT200.006936984652.542
AACATGG450.004293285331.11110921
TAACAGA450.004293285331.1111096
AGTTCAC450.004293285331.11110945
AGCAGTC450.004293285331.11110927
TCTTACG703.4651872E-530.08
CAGTTCA500.007185124327.99999844
TCTCGTT500.007185124327.99999843
TAGATCT1004.369358E-727.99999839
CTGGGTT500.007185124327.99999856