##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780248_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 120276 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.32819515115235 34.0 32.0 34.0 12.0 34.0 2 30.46694269846021 34.0 32.0 34.0 12.0 34.0 3 30.268183178689014 34.0 32.0 34.0 11.0 34.0 4 30.10335395257574 34.0 31.0 34.0 11.0 34.0 5 30.13857294888423 34.0 31.0 34.0 11.0 34.0 6 33.00601117429911 38.0 34.0 38.0 11.0 38.0 7 33.41578536033789 38.0 34.0 38.0 12.0 38.0 8 33.354185373640625 38.0 34.0 38.0 11.0 38.0 9 33.62451361867704 38.0 34.0 38.0 21.0 38.0 10-11 33.359751737670024 38.0 34.0 38.0 11.0 38.0 12-13 33.405658651767595 38.0 34.0 38.0 11.0 38.0 14-15 33.294871794871796 38.0 34.0 38.0 11.0 38.0 16-17 33.36207971665171 38.0 34.0 38.0 11.0 38.0 18-19 33.39729455585487 38.0 34.0 38.0 11.0 38.0 20-21 33.37707855266221 38.0 34.0 38.0 11.0 38.0 22-23 33.39887425587815 38.0 34.0 38.0 11.0 38.0 24-25 33.41789716984269 38.0 34.0 38.0 11.0 38.0 26-27 33.383501280388444 38.0 34.0 38.0 11.0 38.0 28-29 33.45594299777179 38.0 34.0 38.0 11.0 38.0 30-31 33.47686155176427 38.0 34.0 38.0 11.0 38.0 32-33 33.537409790814465 38.0 34.0 38.0 11.0 38.0 34-35 33.47975489707007 38.0 34.0 38.0 11.0 38.0 36-37 33.41553593401842 38.0 34.0 38.0 11.0 38.0 38-39 33.41983022381855 38.0 34.0 38.0 11.0 38.0 40-41 33.466431374505305 38.0 34.0 38.0 11.0 38.0 42-43 33.53797100003325 38.0 34.0 38.0 11.0 38.0 44-45 33.62870398084406 38.0 34.0 38.0 21.0 38.0 46-47 33.55634124846187 38.0 34.0 38.0 11.0 38.0 48-49 33.50834746749143 38.0 34.0 38.0 11.0 38.0 50-51 33.438146429877946 38.0 34.0 38.0 11.0 38.0 52-53 33.52170008979347 38.0 34.0 38.0 11.0 38.0 54-55 33.50865509328544 38.0 34.0 38.0 11.0 38.0 56-57 33.471332601682796 38.0 34.0 38.0 11.0 38.0 58-59 33.49209734277828 38.0 34.0 38.0 11.0 38.0 60-61 33.50489291296684 38.0 34.0 38.0 11.0 38.0 62-63 33.40392929595264 38.0 34.0 38.0 11.0 38.0 64-65 33.358857960025276 38.0 34.0 38.0 11.0 38.0 66-67 33.266645049718974 38.0 34.0 38.0 11.0 38.0 68-69 33.1964980544747 38.0 34.0 38.0 11.0 38.0 70-71 33.16192756659683 38.0 34.0 38.0 11.0 38.0 72-73 33.13223752036981 38.0 34.0 38.0 11.0 38.0 74-75 33.12720326582195 38.0 34.0 38.0 11.0 38.0 76 31.65617413282783 37.0 28.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.19079891881695232 1101 2 -0.09217664554603999 1101 3 0.19932757683466562 1101 4 0.73300217068725 1101 5 -0.3041897246902323 1101 6 -0.4768432384438057 1101 7 0.3616547676338655 1101 8 -0.8564150563445452 1101 9 -0.4560258060191984 1101 10-11 -0.6234823689452682 1101 12-13 0.12239851428562787 1101 14-15 -0.5657751044957635 1101 16-17 -0.41088501758899554 1101 18-19 -0.2006287036629999 1101 20-21 -0.32972664950269603 1101 22-23 0.10622559542304799 1101 24-25 -0.2514244126923302 1101 26-27 -0.8045878945481562 1101 28-29 -0.3569015006269751 1101 30-31 -0.3076798824021978 1101 32-33 -0.475515182261411 1101 34-35 -0.33630925125602573 1101 36-37 -0.4529474780341687 1101 38-39 -0.03517963257196044 1101 40-41 -0.346086073956414 1101 42-43 -0.6444018063895314 1101 44-45 -0.22304583070452821 1101 46-47 -0.5923573775780966 1101 48-49 -0.4532131838948743 1101 50-51 -0.4499560161805505 1101 52-53 -0.4230012813654582 1101 54-55 -0.13366344211777204 1101 56-57 -0.28815853823287796 1101 58-59 -0.828305097449757 1101 60-61 -0.5037639086601189 1101 62-63 -0.8276737700537709 1101 64-65 -0.4256117077790904 1101 66-67 -0.8468633392423612 1101 68-69 -0.7595965998470149 1101 70-71 -0.38696945239733793 1101 72-73 -0.7663398611798371 1101 74-75 -0.560731009455445 1101 76 -1.327052693644049 1102 1 -0.47068908836510204 1102 2 -0.12566866926607645 1102 3 0.3279920719486711 1102 4 0.4555967585098486 1102 5 -0.6018699218003967 1102 6 -0.04609279995720783 1102 7 -0.3877397032589798 1102 8 0.0048665773312279725 1102 9 -0.2683326631143288 1102 10-11 0.046697410558621755 1102 12-13 0.07662886591011642 1102 14-15 -0.2551543327593606 1102 16-17 -0.150914667634936 1102 18-19 -0.3341008910847023 1102 20-21 0.1584446525936869 1102 22-23 0.0744770779253443 1102 24-25 0.004072329978683342 1102 26-27 -0.08648257424413686 1102 28-29 0.033549931762571816 1102 30-31 0.3607302904867993 1102 32-33 0.15517992339297848 1102 34-35 0.04896908333012817 1102 36-37 -0.06413515029675665 1102 38-39 0.07239052528321821 1102 40-41 0.025108488809813423 1102 42-43 -0.004023455099137152 1102 44-45 -0.36724527946424246 1102 46-47 -0.678227669067283 1102 48-49 -0.5208079805206154 1102 50-51 -0.04467434074414456 1102 52-53 -0.3755246673171797 1102 54-55 -0.39109683391598793 1102 56-57 -0.4931197008487658 1102 58-59 -0.02015864292707903 1102 60-61 0.045096026193306216 1102 62-63 -0.14640132371617653 1102 64-65 -0.06893940342763472 1102 66-67 -0.45373400657719287 1102 68-69 -0.15730394136542714 1102 70-71 -0.3492178446131575 1102 72-73 -0.007512708419461944 1102 74-75 -0.03346131931930785 1102 76 -0.16935412185752696 1103 1 0.30752199363855226 1103 2 -0.09724920431410311 1103 3 -0.323710714429037 1103 4 0.37894160252032805 1103 5 0.010349257369245635 1103 6 -0.6357867747131039 1103 7 0.3951054185954348 1103 8 -0.1793141799919482 1103 9 0.0783173329164697 1103 10-11 -0.4962145922992036 1103 12-13 -0.1974046341295761 1103 14-15 -0.42916897910372853 1103 16-17 -0.16334818355142033 1103 18-19 -0.22712601611446814 1103 20-21 -0.13935442655213848 1103 22-23 -0.13246703575251928 1103 24-25 0.21943610706846783 1103 26-27 -0.4016270530883048 1103 28-29 -0.2905649368569385 1103 30-31 -0.47628447493353576 1103 32-33 -0.09506319004673003 1103 34-35 0.09112693837498398 1103 36-37 -0.05978887737463623 1103 38-39 -0.168931813368836 1103 40-41 -0.018220279944657136 1103 42-43 -0.03587627719963393 1103 44-45 0.2637382880518331 1103 46-47 -0.059022699630119746 1103 48-49 0.013391630497885387 1103 50-51 -0.19784076925887462 1103 52-53 0.1339666798289656 1103 54-55 -0.17769284884018077 1103 56-57 0.3482711064863082 1103 58-59 -0.022257356906600023 1103 60-61 -0.010631527986262768 1103 62-63 0.01654421887332802 1103 64-65 0.1657246038756952 1103 66-67 0.31474757633937145 1103 68-69 0.3869327816638588 1103 70-71 0.1628485203943839 1103 72-73 -0.06041716979915179 1103 74-75 0.1313001318198559 1103 76 0.3523474737604779 1104 1 -0.616704992292398 1104 2 -0.5181548750434217 1104 3 -0.5992231179247476 1104 4 -0.008741118802710446 1104 5 -0.9243707332252598 1104 6 -0.3777627123233245 1104 7 -0.25815515278835477 1104 8 -0.7966183301663392 1104 9 -0.2939736887553579 1104 10-11 -0.236226153219377 1104 12-13 -0.4412224810551564 1104 14-15 -0.07227945447309025 1104 16-17 -0.4216465728605101 1104 18-19 -0.8261286418149894 1104 20-21 -0.850115879701022 1104 22-23 -0.2210844280792017 1104 24-25 -0.49671880926182155 1104 26-27 -0.1761450215015259 1104 28-29 -0.8642511068612535 1104 30-31 -0.26439142324251463 1104 32-33 -0.3236216020857654 1104 34-35 -0.04525762877632644 1104 36-37 -0.5287164550679435 1104 38-39 0.09302100240103783 1104 40-41 -0.31550738266049194 1104 42-43 -0.28067876181773244 1104 44-45 -0.3913445978673593 1104 46-47 -0.35889141460444307 1104 48-49 -0.08188798701200994 1104 50-51 -0.5265187094556012 1104 52-53 -0.10919474196826684 1104 54-55 -0.348456457414855 1104 56-57 -0.17678571837554102 1104 58-59 -0.7385739301714764 1104 60-61 -0.274524226971252 1104 62-63 -0.38589337175253036 1104 64-65 -0.6015383972608035 1104 66-67 -0.3260360189108553 1104 68-69 -0.3869777485708852 1104 70-71 -0.27367418995557813 1104 72-73 -0.09780400994493732 1104 74-75 -0.11764664875779829 1104 76 -0.19552257365889147 1105 1 0.25967950137848916 1105 2 0.06167107432366947 1105 3 -0.44388292805133034 1105 4 -0.3645955491824573 1105 5 0.017440975635501843 1105 6 0.1394841231197148 1105 7 0.5213147839205092 1105 8 0.40943388502353173 1105 9 0.1747763112446421 1105 10-11 0.20979837209708307 1105 12-13 -0.8914400443462895 1105 14-15 -0.09710144814397381 1105 16-17 0.13626481954455016 1105 18-19 0.3285193012229968 1105 20-21 0.4415376013116372 1105 22-23 0.3344931035663663 1105 24-25 0.24313482997868618 1105 26-27 0.5520590924225246 1105 28-29 0.061955380480519295 1105 30-31 0.47142740587140963 1105 32-33 0.1251719105724618 1105 34-35 0.5238088269198755 1105 36-37 0.2136292727801603 1105 38-39 0.17407321759090877 1105 40-41 0.06176714265596672 1105 42-43 0.5417698141316336 1105 44-45 0.39922106668959856 1105 46-47 0.6446489334968177 1105 48-49 0.6101215066588708 1105 50-51 0.7724330310507241 1105 52-53 0.7786019352469253 1105 54-55 0.7446804096737623 1105 56-57 0.6941399145358531 1105 58-59 0.5344086647652304 1105 60-61 0.5450960261933062 1105 62-63 0.6274768814120222 1105 64-65 0.6463650837518529 1105 66-67 0.7078445190638263 1105 68-69 0.7812777894038021 1105 70-71 0.6312308733355607 1105 72-73 0.728224471067719 1105 74-75 0.484647655039673 1105 76 1.1727131858347768 1106 1 0.022535514036718496 1106 2 -0.27764854592949817 1106 3 -0.7859319786842391 1106 4 -0.05937403019511578 1106 5 -0.43069984714930953 1106 6 -0.40624372498155736 1106 7 -0.9733450262060757 1106 8 -0.23965630484988054 1106 9 -0.10726482799586279 1106 10-11 -0.5859096975231708 1106 12-13 -0.16590602535895727 1106 14-15 -0.43936806206802714 1106 16-17 -0.19538075007569944 1106 18-19 -0.4273944645998 1106 20-21 -0.4450525885617864 1106 22-23 -0.26064139010451726 1106 24-25 -0.4286808345782802 1106 26-27 -0.40082856580531967 1106 28-29 -0.4971624992663095 1106 30-31 -0.49540408147036175 1106 32-33 -0.1986216020857583 1106 34-35 -0.5373462363712633 1106 36-37 -0.1980202525362884 1106 38-39 -0.38957393430782616 1106 40-41 -0.2126592813946644 1106 42-43 -0.10662812890633688 1106 44-45 -0.4530534586268473 1106 46-47 -0.6025623006803968 1106 48-49 -0.40150824017656817 1106 50-51 -0.510695924645475 1106 52-53 -0.2373592989302864 1106 54-55 -0.5857982295667483 1106 56-57 -0.10241862976795346 1106 58-59 -0.2844600061208453 1106 60-61 -0.44066346747757734 1106 62-63 -0.22450096668923436 1106 64-65 -0.07147510612156793 1106 66-67 -0.4146436138475593 1106 68-69 -0.33792711565949674 1106 70-71 -0.4651298861581168 1106 72-73 -0.189576161843668 1106 74-75 -0.3375833576185556 1106 76 0.31397109722718497 1107 1 -0.23787320946488322 1107 2 -0.5989463955558492 1107 3 -0.1418874461236186 1107 4 -1.2421557901463132 1107 5 -0.4228075183404023 1107 6 -0.6817508166393225 1107 7 0.13859641042652981 1107 8 -0.29135677762706536 1107 9 -0.09216645983969585 1107 10-11 -0.19733641705954597 1107 12-13 0.08805920264166645 1107 14-15 -0.4164726830837324 1107 16-17 -0.21945807202170897 1107 18-19 -0.4603172951625538 1107 20-21 0.01529513143211858 1107 22-23 0.17843135657841458 1107 24-25 -0.16083730857553746 1107 26-27 0.16000336953096195 1107 28-29 -0.1833947701218932 1107 30-31 -0.49225858810449097 1107 32-33 0.2229316997290951 1107 34-35 -0.09976045018856183 1107 36-37 -0.43021485372586454 1107 38-39 -0.5959945534934263 1107 40-41 0.01705705229451837 1107 42-43 0.015207314131636451 1107 44-45 -0.034645650177864695 1107 46-47 0.29331233711127425 1107 48-49 0.4415221090685151 1107 50-51 0.16974101297843447 1107 52-53 -0.32793044427114637 1107 54-55 -0.09178419875998145 1107 56-57 -0.3506266517292147 1107 58-59 -0.25388199788537236 1107 60-61 0.008951447880050978 1107 62-63 -0.1039237209976136 1107 64-65 0.43254731266751634 1107 66-67 0.12235882629273931 1107 68-69 -0.13135398770463524 1107 70-71 0.19252605405845458 1107 72-73 -0.03359781808890716 1107 74-75 0.10354825744931162 1107 76 0.27237433041309345 1108 1 -0.08818234072677456 1108 2 -0.1879999783079107 1108 3 -0.8266131912092227 1108 4 -0.414595549182458 1108 5 0.03898702826707989 1108 6 -0.1460421926697606 1108 7 -0.2624023213426483 1108 8 -0.6808621676080477 1108 9 -1.0505526361237756 1108 10-11 -0.09702728579765818 1108 12-13 -0.5474433338199773 1108 14-15 -0.5991573691966039 1108 16-17 -0.05189307519228947 1108 18-19 -0.22731951456648147 1108 20-21 -0.2925084513199394 1108 22-23 -0.5577766332757434 1108 24-25 -0.775532933179214 1108 26-27 -0.4000790654722124 1108 28-29 0.016807354164733113 1108 30-31 -0.5962535151812176 1108 32-33 -0.27211427363805285 1108 34-35 -0.6432964362380176 1108 36-37 -0.7364529640619466 1108 38-39 -0.30273599293540343 1108 40-41 -0.41808483102823857 1108 42-43 -0.5785426858683635 1108 44-45 -0.0918151175209232 1108 46-47 -0.23191356650318085 1108 48-49 -0.36915480913060605 1108 50-51 -0.48208999526506346 1108 52-53 -0.7630921437004474 1108 54-55 -0.3580334061157231 1108 56-57 -0.5010903486220428 1108 58-59 -0.3884531773400326 1108 60-61 -0.7361539738066938 1108 62-63 -0.6304178554300748 1108 64-65 -0.6294572846692006 1108 66-67 -0.21921140198880096 1108 68-69 -0.6633768158593583 1108 70-71 -0.22008491613812708 1108 72-73 -0.1076307920901769 1108 74-75 -0.16107602917085018 1108 76 -0.1149512878494292 1109 1 0.07222787599149072 1109 2 0.3392833034258338 1109 3 0.019942536344956352 1109 4 0.07553602976491192 1109 5 0.025829133530237414 1109 6 0.7512101293116658 1109 7 0.8161580501743799 1109 8 0.5884876775931929 1109 9 0.7594318840000582 1109 10-11 0.6353643550692212 1109 12-13 0.4248863875422515 1109 14-15 0.49033566485912417 1109 16-17 0.2298406709377474 1109 18-19 0.6490349745976047 1109 20-21 0.6145155424881068 1109 22-23 0.6121150075911359 1109 24-25 0.40519462100035497 1109 26-27 0.533357467035529 1109 28-29 0.34410998574367824 1109 30-31 0.8255731102057808 1109 32-33 0.3213454786839236 1109 34-35 0.6685263191799322 1109 36-37 0.19408109166561616 1109 38-39 0.6855573507178505 1109 40-41 0.4554639305816579 1109 42-43 0.6142785215619782 1109 44-45 0.2916020651416176 1109 46-47 0.6932992817940331 1109 48-49 0.41277243545144415 1109 50-51 0.5033976208340079 1109 52-53 0.4466601782809789 1109 54-55 0.31456721307932867 1109 56-57 0.30802342846773456 1109 58-59 0.4885785564060896 1109 60-61 0.6023715679889747 1109 62-63 0.5954915872943758 1109 64-65 0.7647958113060298 1109 66-67 0.433942622779 1109 68-69 0.7104621934285689 1109 70-71 0.8222912448525861 1109 72-73 0.5494899509438795 1109 74-75 0.9408976550396702 1109 76 0.3771152756180598 1110 1 -0.04761216528817869 1110 2 -0.003873797471204199 1110 3 0.34081258476918563 1110 4 0.25367368158677195 1110 5 -0.16597248590295877 1110 6 -0.12622100508541934 1110 7 -0.019149959669235272 1110 8 0.04653324399789227 1110 9 0.3470519522702844 1110 10-11 -0.25458464072342935 1110 12-13 -0.11888395460270118 1110 14-15 -0.3881671532721782 1110 16-17 -0.2230300522503228 1110 18-19 0.08096321147939989 1110 20-21 -0.013029706380670802 1110 22-23 0.2876181035663663 1110 24-25 0.18355950946586574 1110 26-27 -0.017572317833881357 1110 28-29 0.15374223945487842 1110 30-31 0.09149952125602567 1110 32-33 0.10389787211092738 1110 34-35 -0.03917194231090093 1110 36-37 -0.13144284260445005 1110 38-39 -0.4260069106142126 1110 40-41 0.005877719579046925 1110 42-43 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6 0.34981021007623525 2116 7 0.43484351062858195 2116 8 0.47016758067570663 2116 9 -0.1402469806808213 2116 10-11 0.5621954994262737 2116 12-13 0.5840743190077475 2116 14-15 0.7750355860175162 2116 16-17 0.861885937556977 2116 18-19 0.49368816768262036 2116 20-21 0.6716035951004571 2116 22-23 0.7717019544980488 2116 24-25 0.7257338206619153 2116 26-27 0.6477306762734614 2116 28-29 0.48304427799605065 2116 30-31 0.6911770176726577 2116 32-33 0.4618469882122156 2116 34-35 0.5243134853049654 2116 36-37 0.7595628907925871 2116 38-39 0.6532851741126535 2116 40-41 0.045026071227397324 2116 42-43 0.4453315377341198 2116 44-45 0.5208340325281142 2116 46-47 0.7791500980930977 2116 48-49 0.9325982147334031 2116 50-51 0.7840304689389228 2116 52-53 0.5473034104022076 2116 54-55 0.8090146488041938 2116 56-57 0.6797183306849206 2116 58-59 0.4649404970633597 2116 60-61 0.45658670942312085 2116 62-63 0.6355902354492997 2116 64-65 0.25247921418663566 2116 66-67 0.685649255088677 2116 68-69 0.3405460347454152 2116 70-71 0.9463706248883526 2116 72-73 0.8649092536764158 2116 74-75 0.4905870960334582 2116 76 0.876692223101859 2117 1 -0.3778204986215101 2117 2 -0.8227039256763327 2117 3 -0.14388292805132963 2117 4 0.01040445081754271 2117 5 -0.29193402436449745 2117 6 -0.46676587688028803 2117 7 0.18381478392051065 2117 8 -0.618691114976464 2117 9 -0.7283486887553607 2117 10-11 0.14886087209708165 2117 12-13 -0.4711275443462952 2117 14-15 -0.1986639481439738 2117 16-17 -0.476235180455447 2117 18-19 -0.3777306987770075 2117 20-21 -0.45221239868836705 2117 22-23 -0.5483193964336337 2117 24-25 0.06657232997868334 2117 26-27 -0.17137840757747114 2117 28-29 -0.4474196195194793 2117 30-31 -0.39576009412859037 2117 32-33 -0.11545308942753252 2117 34-35 -0.3199411730801245 2117 36-37 -0.11762072721983685 2117 38-39 -0.3306142824090941 2117 40-41 -0.08979535734403044 2117 42-43 -0.4738551858683664 2117 44-45 -0.27265393331039434 2117 46-47 -0.32566356650318085 2117 48-49 -0.08519099334112923 2117 50-51 -0.16037946894927302 2117 52-53 -0.1010855647530775 2117 54-55 -0.02250709032624343 2117 56-57 -0.5277350854641512 2117 58-59 -0.004653835234769588 2117 60-61 -0.031466473806695205 2117 62-63 -0.08189811858797214 2117 64-65 -0.9301974162481486 2117 66-67 -0.25934298093616803 2117 68-69 -0.3515347105961979 2117 70-71 -0.565644126664445 2117 72-73 -1.3452130289322781 2117 74-75 -0.8075398449603242 2117 76 -0.14916181416522178 2118 1 0.2455835533662558 2118 2 0.27847153303926575 2118 3 -0.5083993653601979 2118 4 -0.3379518182956076 2118 5 -0.2935013026519613 2118 6 0.20687708948057093 2118 7 -0.05193636286848147 2118 8 -0.38889371436484765 2118 9 -0.07302567652293845 2118 10-11 -0.4479950147530687 2118 12-13 -0.36573763608941334 2118 14-15 4.472903881378443E-4 2118 16-17 0.07976634859653586 2118 18-19 -0.07699962079535538 2118 20-21 -0.05835727636420529 2118 22-23 0.19606611579877864 2118 24-25 0.27108303334259887 2118 26-27 -0.420198170574416 2118 28-29 0.1944238437832695 2118 30-31 0.30906597620780474 2118 32-33 0.06609794268256053 2118 34-35 -0.36283108133700637 2118 36-37 -0.176990948932378 2118 38-39 -0.4717314460176496 2118 40-41 0.026240575377677544 2118 42-43 -0.07347770115888608 2118 44-45 -0.04708531710245012 2118 46-47 -0.08063298546342423 2118 48-49 0.05945254641422082 2118 50-51 0.11668475123421018 2118 52-53 -0.28614863814757285 2118 54-55 0.06426660997956901 2118 56-57 0.33174503686000634 2118 58-59 0.1550795363248625 2118 60-61 0.1720073411780163 2118 62-63 -0.2581691430528039 2118 64-65 -0.05519263796068685 2118 66-67 -0.11253487696063758 2118 68-69 -0.13372125493870612 2118 70-71 -0.07775230709257386 2118 72-73 0.1190310459912709 2118 74-75 -0.28387298716216236 2118 76 -0.015713801932804472 2119 1 -0.2591302119973058 2119 2 -0.41392605943429217 2119 3 -0.7165939153124761 2119 4 -0.3019363135136679 2119 5 -0.006185616721186449 2119 6 -0.4311368959885655 2119 7 -0.31138824155719647 2119 8 0.02195378948213289 2119 9 -0.05352782888274987 2119 10-11 -0.46031508331693516 2119 12-13 -0.32088869084311256 2119 14-15 -0.5667969099274188 2119 16-17 -0.29828932058283897 2119 18-19 -0.9041339631082224 2119 20-21 -0.6197482267138454 2119 22-23 -0.4930347626756699 2119 24-25 -0.8546060139703613 2119 26-27 -0.7151980731825702 2119 28-29 -0.40706332971056725 2119 30-31 -0.7699042024088385 2119 32-33 -0.5395673410198896 2119 34-35 -0.8885717463285374 2119 36-37 -0.5901028132071033 2119 38-39 -0.5024196168039907 2119 40-41 -0.5921440834586775 2119 42-43 -0.7252385457409716 2119 44-45 -0.4573971657944682 2119 46-47 -0.2802814008980903 2119 48-49 -0.8532940984366633 2119 50-51 -0.8508253288218839 2119 52-53 -0.7024987813135866 2119 54-55 -0.3895453068867525 2119 56-57 -0.6229580154004566 2119 58-59 -0.9127051887379523 2119 60-61 -0.6316555661633814 2119 62-63 -0.596527895658042 2119 64-65 -1.0590688334455933 2119 66-67 -0.9324719618278934 2119 68-69 -0.8150697424433346 2119 70-71 -1.1137532030975663 2119 72-73 -0.5282643824354665 2119 74-75 -1.2087838736227496 2119 76 -1.021693661298979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 38.0 13 379.0 14 1124.0 15 1787.0 16 2358.0 17 2660.0 18 2779.0 19 2566.0 20 1954.0 21 1490.0 22 1066.0 23 901.0 24 787.0 25 842.0 26 856.0 27 966.0 28 1230.0 29 1417.0 30 1769.0 31 2137.0 32 2829.0 33 3583.0 34 5113.0 35 7729.0 36 14806.0 37 57110.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.78373075260235 21.098972363563803 13.879743257175164 28.237553626658684 2 18.89154943629652 21.537131264757726 33.27263959559679 26.298679703348967 3 18.16405600452293 24.193521567062422 28.225082310685423 29.417340117729225 4 14.72030995377299 19.44111876018491 31.63806578203465 34.20050550400745 5 14.939805114902391 32.57590874322392 33.271808174531905 19.212477967341783 6 28.438757524360636 32.90930859024244 18.92231201569723 19.72962186969969 7 26.686121919584956 27.6929728291596 21.609464897402642 24.011440353852805 8 23.59905550567029 31.32960856696265 21.19791147028501 23.873424457082045 9 22.765971598656424 17.52136752136752 21.257773786956665 38.454887093019394 10-11 25.793175695899436 24.06298845987562 25.047806711230834 25.09602913299411 12-13 24.659533073929964 21.51551431707074 24.778010575675946 29.046942033323358 14-15 23.577438557983303 22.753915993215603 22.57058764840866 31.098057800392432 16-17 24.16816322458346 24.418420965113572 23.65767069074462 27.75574511955835 18-19 23.174199341514516 23.784878113671887 24.970900262729057 28.07002228208454 20-21 23.57868568958063 24.092919618211447 24.690711363863116 27.63768332834481 22-23 24.684059995344043 23.575360005321095 24.24756393627989 27.493016063054977 24-25 24.08917822341947 24.074628354783997 23.618593900695068 28.217599521101466 26-27 23.35887192320963 24.5731674364272 24.345357117617468 27.722603522745697 28-29 23.840583325019125 23.893378562639263 25.813129801456654 26.452908310884965 30-31 23.739981376168146 23.760766902790248 25.242359240413712 27.256892480627886 32-33 23.262745684924674 24.27375369982374 25.026189763543847 27.437310851707743 34-35 23.330506501712726 23.707555954637666 25.91830456616449 27.043632977485117 36-37 23.841098145507605 22.998449393267954 25.68395059675495 27.476501864469487 38-39 22.50324254215305 23.16921081512521 27.68673717117297 26.64080947154877 40-41 22.457930094116865 22.932255811633244 27.724151119092756 26.88566297515714 42-43 21.36793707805381 22.864494994845188 27.884199674082943 27.88336825301806 44-45 20.620905251255444 22.863247863247864 28.952575742459015 27.56327114303768 46-47 20.94973228241711 23.305979580298647 29.027403638298583 26.716884498985667 48-49 20.53069606571552 23.860537430576343 28.62790581662177 26.980860687086366 50-51 20.30745950979414 24.594266520336557 27.8027204097243 27.295553560145 52-53 19.626525657654064 24.578885230636203 28.054640992384183 27.739948119325554 54-55 19.44527586550933 24.34276164820912 27.92992783265157 28.282034653629985 56-57 20.03807908477169 24.435049386411254 27.458512088862285 28.068359439954772 58-59 20.304549536067046 24.037214406864212 27.14215637367388 28.516079683394857 60-61 20.179836376334432 23.64353653264159 27.45518640460275 28.72144068642123 62-63 20.009395058033192 23.74122850776547 27.01993747713592 29.22943895706542 64-65 20.70903588413316 23.954903721440687 25.97026838271975 29.36579201170641 66-67 20.450879643486648 24.620040573347968 25.210349529415677 29.71873025374971 68-69 20.67079051514849 24.780920549403042 25.069839369450264 29.478449565998204 70-71 21.324287472147393 24.524011440353853 25.110579001629585 29.04112208586917 72-73 21.56082676510692 23.439838371744987 25.835993880740965 29.16334098240713 74-75 21.782816189430974 23.268565632378863 26.377248993980512 28.57136918420965 76 21.648541687452195 22.60883301739333 26.6628421297682 29.07978316538628 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 1.5 15 2.0 16 3.0 17 4.0 18 7.0 19 13.5 20 30.5 21 44.0 22 56.5 23 101.5 24 138.0 25 142.0 26 206.0 27 320.5 28 470.0 29 569.0 30 674.0 31 939.0 32 1369.5 33 1640.0 34 1828.5 35 2292.5 36 2787.0 37 3006.0 38 3372.5 39 4187.5 40 5046.5 41 5687.0 42 5917.0 43 6194.0 44 6624.0 45 6797.0 46 6817.0 47 6817.0 48 6404.5 49 5800.5 50 5609.0 51 5244.5 52 4389.0 53 3638.5 54 3379.0 55 3227.0 56 2885.0 57 2662.0 58 2629.0 59 2503.5 60 2486.0 61 2756.0 62 2918.0 63 2851.0 64 2890.5 65 2917.5 66 2799.0 67 2760.0 68 2617.5 69 2279.5 70 1872.5 71 1661.0 72 1469.5 73 1085.5 74 721.5 75 550.0 76 411.0 77 191.0 78 80.5 79 51.0 80 30.0 81 7.0 82 5.5 83 6.0 84 6.0 85 7.0 86 6.5 87 5.0 88 4.5 89 6.0 90 7.0 91 3.5 92 1.0 93 2.0 94 2.0 95 1.0 96 1.0 97 1.5 98 4.5 99 17.5 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.1571053244204994E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.1571053244204994E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 120276.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.98702983138781 #Duplication Level Percentage of deduplicated Percentage of total 1 74.97158697158697 44.973228241710736 2 12.835758835758837 15.399580963783299 3 4.885654885654886 8.792277761149357 4 2.2577962577962576 5.417539658784795 5 1.3624393624393625 4.086434533905351 6 0.9092169092169092 3.2724733113838176 7 0.6347886347886348 2.665535934018424 8 0.46708246708246703 2.2415111909275334 9 0.36174636174636177 1.9530080814127506 >10 1.313929313929314 11.198410322923943 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0074827895839568994 0.0 2 0.0 0.0 0.0 0.009977052778609198 0.0 3 0.0 0.0 0.0 0.013302737038145598 0.0 4 0.0 0.0 0.0 0.049885263893046 0.0 5 0.0 0.0 0.0 0.0557052113472347 0.0 6 0.0 0.0 0.0 0.0557052113472347 0.0 7 0.0 0.0 0.0 0.0557052113472347 0.0 8 0.0 0.0 0.0 0.05903089560677109 0.0 9 0.0 0.0 0.0 0.06401942199607569 0.0 10 0.0 0.0 0.0 0.0698393694502644 0.0 11 0.0 0.0 0.0 0.0723336326449167 0.0 12 0.0 0.0 0.0 0.07399647477468489 0.0 13 0.0 0.0 0.0 0.07399647477468489 0.0 14 0.0 0.0 0.0 0.07981642222887358 0.0 15 0.0 0.0 0.0 0.0806478432937577 0.0 16 0.0 0.0 0.0 0.08314210648840999 0.0 17 0.0 0.0 0.0 0.08563636968306229 0.0 18 0.0 0.0 0.0 0.09145631713725098 0.0 19 0.0 0.0 0.0 0.10226479098074429 0.0 20 0.0 0.0 0.0 0.10475905417539659 0.0 21 0.0 0.0 0.0 0.10559047524028069 0.0 22 0.0 0.0 0.0 0.11972463334331039 0.0 23 0.0 0.0 0.0 0.12637600186238318 0.0 24 0.0 0.0 0.0 0.13635305464099237 0.0 25 0.0 0.0 0.0 0.14383584422494927 0.0 26 0.0 0.0 0.0 0.1538128970035585 0.0 27 0.0 0.0 0.0 0.2643918986331438 0.0 28 0.0 0.0 0.0 0.37663374239249725 0.0 29 0.0 0.0 0.0 0.48804416508696663 0.0 30 0.0 0.0 0.0 0.5795004822242177 0.0 31 0.0 0.0 0.0 0.7291562739033556 0.0 32 0.0 0.0 0.0 0.8788120655824936 0.0 33 0.0 0.0 0.0 1.0633875419867638 0.0 34 0.0 0.0 0.0 1.3577105989557352 0.0 35 0.0 0.0 0.0 1.7534670258405667 0.0 36 0.0 0.0 0.0 2.3096877182480293 0.0 37 0.0 0.0 0.0 3.0604609398383715 0.0 38 0.0 0.0 0.0 3.894376267917124 0.0 39 0.0 0.0 0.0 4.647643752702119 0.0 40 0.0 0.0 0.0 5.4707506069373775 0.0 41 0.0 0.0 0.0 6.330439988027536 0.0 42 0.0 0.0 0.0 7.200106421896305 0.0 43 0.0 0.0 0.0 8.12381522498254 0.0 44 0.0 0.0 0.0 9.00595297482457 0.0 45 0.0 0.0 0.0 9.888922145731485 0.0 46 0.0 0.0 0.0 10.80265389603911 0.0 47 0.0 0.0 0.0 11.663174698194153 0.0 48 0.0 0.0 0.0 12.394825235292162 0.0 49 0.0 0.0 0.0 13.122318667065748 0.0 50 0.0 8.314210648840999E-4 0.0 13.829026572217234 0.0 51 0.0 8.314210648840999E-4 0.0 14.550700056536632 0.0 52 0.0 8.314210648840999E-4 0.0 15.318933120489541 0.0 53 0.0 8.314210648840999E-4 0.0 16.04060660480894 0.0 54 0.0 8.314210648840999E-4 0.0 16.70740629884599 0.0 55 0.0 8.314210648840999E-4 0.0 17.360071834780005 0.0 56 0.0 8.314210648840999E-4 0.0 18.06262263460707 0.0 57 0.0 8.314210648840999E-4 0.0 18.69283980178922 0.0 58 0.0 8.314210648840999E-4 0.0 19.35631381156673 0.0 59 0.0 8.314210648840999E-4 0.0 19.994013768332835 0.0 60 0.0 8.314210648840999E-4 0.0 20.598456882503577 0.0 61 0.0 8.314210648840999E-4 0.0 21.164654627689647 0.0 62 0.0 8.314210648840999E-4 0.0 21.78738900528784 0.0 63 0.0 8.314210648840999E-4 0.0 22.386843593069273 0.0 64 0.0 8.314210648840999E-4 0.0 22.987129601915594 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCACAA 15 0.0022200495 70.00001 33 TGTGTCC 15 0.0022200495 70.00001 7 TAGCTAC 25 2.3824078E-4 55.999996 54 GATACAG 20 0.0069369846 52.5 40 CGTGAAA 20 0.0069369846 52.5 17 AGGAACC 20 0.0069369846 52.5 25 GCACAAG 20 0.0069369846 52.5 34 GGAACCA 20 0.0069369846 52.5 26 CAAGAGT 20 0.0069369846 52.5 37 GCTACAG 20 0.0069369846 52.5 3 TACAGTT 20 0.0069369846 52.5 42 AACATGG 45 0.0042932853 31.111109 21 TAACAGA 45 0.0042932853 31.111109 6 AGTTCAC 45 0.0042932853 31.111109 45 AGCAGTC 45 0.0042932853 31.111109 27 TCTTACG 70 3.4651872E-5 30.0 8 CAGTTCA 50 0.0071851243 27.999998 44 TCTCGTT 50 0.0071851243 27.999998 43 TAGATCT 100 4.369358E-7 27.999998 39 CTGGGTT 50 0.0071851243 27.999998 56 >>END_MODULE