FastQCFastQC Report
Wed 25 May 2016
SRR1780248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780248_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120276
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT86917.2258804749077115No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC24912.0710698726262926No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG22851.8997971332601682No Hit
CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG20531.706907446207057Illumina PCR Primer Index 8 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC4740.39409358475506334No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2830.23529216136220027RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT1460.12138747547307858No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1250.1039276331105125No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATAC301.01650585E-770.000012
CTTAATA352.9534567E-760.01
TCTTTAT900.058.333331
AAATTCT252.3824078E-455.99999652
CTTTATA950.055.2631572
CATATTC200.006936984652.53
CTACTGG200.006936984652.59
TTGTGCG200.006936984652.521
TGAAGTT200.006936984652.539
ACGTTAC200.006936984652.569
ACGCACT200.006936984652.58
GACGTTC200.006936984652.522
GGCGTAA200.006936984652.513
GGATATT200.006936984652.528
CGTTATC200.006936984652.510
CGTTACT200.006936984652.570
TCTAACT200.006936984652.521
TTCGGTC200.006936984652.528
CGGTATA200.006936984652.560
TTCTGAT200.006936984652.519
Produced by FastQC (version 0.11.5)