##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780248_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 120276 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.73045329076457 34.0 34.0 34.0 34.0 34.0 2 33.7604010775217 34.0 34.0 34.0 34.0 34.0 3 33.789334530579666 34.0 34.0 34.0 34.0 34.0 4 33.78624995842895 34.0 34.0 34.0 34.0 34.0 5 33.7805214672919 34.0 34.0 34.0 34.0 34.0 6 37.563587083042336 38.0 38.0 38.0 37.0 38.0 7 37.554832219229105 38.0 38.0 38.0 37.0 38.0 8 37.478058798097706 38.0 38.0 38.0 37.0 38.0 9 37.510966443845824 38.0 38.0 38.0 37.0 38.0 10-11 37.46182945891117 38.0 38.0 38.0 37.0 38.0 12-13 37.43581845089627 38.0 38.0 38.0 37.0 38.0 14-15 37.363572117463164 38.0 38.0 38.0 37.0 38.0 16-17 37.39715737137916 38.0 38.0 38.0 37.0 38.0 18-19 37.36114021084838 38.0 38.0 38.0 36.5 38.0 20-21 37.36765023778642 38.0 38.0 38.0 37.0 38.0 22-23 37.35912401476604 38.0 38.0 38.0 37.0 38.0 24-25 37.38173451062556 38.0 38.0 38.0 37.0 38.0 26-27 37.27276430875652 38.0 38.0 38.0 36.0 38.0 28-29 37.23692174664937 38.0 38.0 38.0 36.0 38.0 30-31 37.245040573347964 38.0 38.0 38.0 36.0 38.0 32-33 37.17123948252353 38.0 38.0 38.0 36.0 38.0 34-35 37.172025175429845 38.0 38.0 38.0 36.0 38.0 36-37 37.18890718015231 38.0 38.0 38.0 36.0 38.0 38-39 36.74363131464299 38.0 37.5 38.0 34.0 38.0 40-41 36.9709418337823 38.0 38.0 38.0 35.5 38.0 42-43 37.1581778575942 38.0 38.0 38.0 36.0 38.0 44-45 37.21365858525391 38.0 38.0 38.0 36.0 38.0 46-47 37.16864960590642 38.0 38.0 38.0 36.0 38.0 48-49 37.176485749442946 38.0 38.0 38.0 36.0 38.0 50-51 37.055551398450234 38.0 38.0 38.0 36.0 38.0 52-53 37.067473976520674 38.0 38.0 38.0 36.0 38.0 54-55 37.03089976387642 38.0 38.0 38.0 36.0 38.0 56-57 37.07056686288204 38.0 38.0 38.0 36.0 38.0 58-59 36.97070072167348 38.0 38.0 38.0 36.0 38.0 60-61 36.974142804882106 38.0 38.0 38.0 36.0 38.0 62-63 36.951274568492465 38.0 38.0 38.0 36.0 38.0 64-65 36.862561940869334 38.0 38.0 38.0 35.5 38.0 66-67 36.54080614586451 38.0 37.5 38.0 34.5 38.0 68-69 36.44416176128239 38.0 37.0 38.0 34.0 38.0 70-71 35.73097292893013 38.0 37.0 38.0 30.5 38.0 72-73 34.5060776879843 38.0 36.5 38.0 22.5 38.0 74-75 34.12002394492667 38.0 36.0 38.0 21.0 38.0 76 33.548297249659115 38.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1052171774892301 1101 2 -0.03919437735450515 1101 3 0.06263977751354588 1101 4 0.04680001350560303 1101 5 -0.12191069284931189 1101 6 -0.12895163971068513 1101 7 0.1291465315919922 1101 8 -0.07889588346332488 1101 9 0.14811782500083126 1101 10-11 0.12885973059045597 1101 12-13 0.11658206737904919 1101 14-15 0.12245784591821263 1101 16-17 0.07567762289608027 1101 18-19 0.10466503757804446 1101 20-21 0.13923263196914348 1101 22-23 -0.004057004765506633 1101 24-25 -0.0017475120534271582 1101 26-27 0.057104722095211 1101 28-29 -0.0734665454815655 1101 30-31 0.07052556408171284 1101 32-33 -0.16692055808221795 1101 34-35 -0.09441028407437102 1101 36-37 0.08528035786864052 1101 38-39 0.052207436218964176 1101 40-41 -0.031507967021461525 1101 42-43 0.19115366093488717 1101 44-45 0.1752918795416818 1101 46-47 0.16610325758959021 1101 48-49 0.1313265199039293 1101 50-51 0.03629272390578109 1101 52-53 0.10021032943470232 1101 54-55 0.047782267239469434 1101 56-57 0.00710948378794285 1101 58-59 -0.06653897028143518 1101 60-61 -0.12225160692736381 1101 62-63 0.19471918273574573 1101 64-65 -0.2585130691145139 1101 66-67 -0.9665295914377623 1101 68-69 -0.9392638148514934 1101 70-71 -0.6134888472090836 1101 72-73 -0.8357381982356813 1101 74-75 -0.5828908311168064 1101 76 -1.239343503678917 1102 1 0.047752003167246926 1102 2 0.03275918482707141 1102 3 0.02048242346694451 1102 4 -0.03723281034806547 1102 5 -0.010608178859499162 1102 6 -0.059336672701576276 1102 7 0.03154150361236674 1102 8 -0.05641822539934793 1102 9 -0.09619228942328562 1102 10-11 0.034136185948341335 1102 12-13 0.12347257101222198 1102 14-15 -0.01869829707018056 1102 16-17 -0.10042172563812102 1102 18-19 -0.08063208929074506 1102 20-21 0.059052226055833046 1102 22-23 0.014448173552374044 1102 24-25 -0.07195756801267805 1102 26-27 0.05017767791246541 1102 28-29 0.07169861780249676 1102 30-31 0.052693775891619055 1102 32-33 0.25473650363998956 1102 34-35 0.07914688622546606 1102 36-37 0.11467984003685672 1102 38-39 -0.11918340149923523 1102 40-41 0.12180573881668266 1102 42-43 0.0057416923388871055 1102 44-45 0.08091286008123433 1102 46-47 -0.04603029289901173 1102 48-49 0.1646201806405898 1102 50-51 -0.17833602411059957 1102 52-53 0.052754595700470475 1102 54-55 0.09794200164187572 1102 56-57 0.2879862481744837 1102 58-59 -0.042197744189401476 1102 60-61 -0.09751887494707034 1102 62-63 0.2726288544972135 1102 64-65 -0.09317752246789013 1102 66-67 0.1432643198626593 1102 68-69 0.054534731878639775 1102 70-71 -0.35010978598801046 1102 72-73 -0.5115139019022621 1102 74-75 -0.4013573808537174 1102 76 -0.22665871289966333 1103 1 -0.11597745622784572 1103 2 0.09281316593368416 1103 3 0.0029187178227445543 1103 4 -0.007374510752924834 1103 5 0.062375468040556825 1103 6 -0.033894107011334995 1103 7 0.07945965872866623 1103 8 0.1243291747752977 1103 9 0.03877040010596744 1103 10-11 0.07383809910226802 1103 12-13 0.18586837953805713 1103 14-15 0.14682226338072013 1103 16-17 0.042186633494999626 1103 18-19 0.069368903009007 1103 20-21 -0.17362222024090102 1103 22-23 0.1584060603508206 1103 24-25 0.008785862962952251 1103 26-27 0.0592224901693541 1103 28-29 0.06966936480574759 1103 30-31 0.022527863372765466 1103 32-33 -0.10908027813344745 1103 34-35 -0.25113016386581677 1103 36-37 -0.03313313130553297 1103 38-39 -0.05063037300038786 1103 40-41 0.19006603888812634 1103 42-43 0.16819604655020726 1103 44-45 -0.09011992550263415 1103 46-47 -0.13078261488043808 1103 48-49 -0.016226449509446184 1103 50-51 -0.16954424829095416 1103 52-53 0.01637192522336761 1103 54-55 -0.08107383546219893 1103 56-57 -0.1327438859844463 1103 58-59 0.056997500710181725 1103 60-61 0.07370244759003697 1103 62-63 -0.007184791608999319 1103 64-65 0.08566962367801523 1103 66-67 -0.15161243650790368 1103 68-69 -0.1522406908410474 1103 70-71 -0.1198118976018776 1103 72-73 -0.023337153470095018 1103 74-75 -0.021147807145695197 1103 76 0.29212552144184656 1104 1 -0.06690232984151834 1104 2 -0.04676045165459186 1104 3 0.01683101158768352 1104 4 0.05125307085933173 1104 5 -0.032962933809194794 1104 6 0.04793369406245773 1104 7 0.0018839249041562312 1104 8 -0.2737583747015222 1104 9 -0.01265609987444094 1104 10-11 -8.767968494538536E-4 1104 12-13 -9.188603412368934E-4 1104 14-15 0.0019727512907437017 1104 16-17 -0.1914027382963539 1104 18-19 -0.09838200814826337 1104 20-21 0.060025935890301696 1104 22-23 -0.0012326119068930552 1104 24-25 -0.10912082669491951 1104 26-27 -0.1390668855409558 1104 28-29 -0.24364136921470703 1104 30-31 -0.16148181644853565 1104 32-33 -0.33431737497085123 1104 34-35 0.004860940104087774 1104 36-37 -0.0013944864805068846 1104 38-39 -0.02897324245996913 1104 40-41 -0.10713778601292034 1104 42-43 -0.0709582427471247 1104 44-45 0.03604106520943873 1104 46-47 -0.03337206505091217 1104 48-49 0.02878765873211364 1104 50-51 -0.23533862067015576 1104 52-53 -0.0815970758347433 1104 54-55 -0.22319967313904243 1104 56-57 -0.15953484887193525 1104 58-59 -0.05524139884698087 1104 60-61 -0.16880254907884762 1104 62-63 -0.21112236264007578 1104 64-65 -0.29410660393494936 1104 66-67 -0.5644401592025758 1104 68-69 -0.4199053849665333 1104 70-71 -0.4444582118237719 1104 72-73 -0.024395271587430045 1104 74-75 -0.4064693574846814 1104 76 -0.2705973691800949 1105 1 -0.0068153045250625155 1105 2 0.06945790277578823 1105 3 0.02889588500540441 1105 4 -0.028098194963455114 1105 5 0.10774118011485712 1105 6 0.1587723016573932 1105 7 0.0667177856636485 1105 8 0.02058497972885931 1105 9 0.07504168493568386 1105 10-11 0.049360544922699034 1105 12-13 -0.040950505910856805 1105 14-15 0.1252520234426342 1105 16-17 0.026140774361884667 1105 18-19 0.0905618209656609 1105 20-21 0.03910030297891609 1105 22-23 -0.0493338777296799 1105 24-25 -0.28910500391010885 1105 26-27 0.12173216509195584 1105 28-29 0.08327714344351733 1105 30-31 0.020321981019826296 1105 32-33 0.19448009338357508 1105 34-35 -0.10663035736427418 1105 36-37 0.041683045165058275 1105 38-39 0.315231662603324 1105 40-41 -0.07567022272178292 1105 42-43 -0.208240679455983 1105 44-45 0.00947856520944157 1105 46-47 -0.04446779289901315 1105 48-49 0.026038449871350622 1105 50-51 -0.21591615231572092 1105 52-53 0.05459754441841369 1105 54-55 0.11056219394956912 1105 56-57 0.24319458150781514 1105 58-59 0.19922853786188455 1105 60-61 -8.041313573272646E-4 1105 62-63 0.1906577006510659 1105 64-65 -0.15094995836533087 1105 66-67 -0.2028895262911874 1105 68-69 0.23314050110941054 1105 70-71 0.5166770729863543 1105 72-73 1.2521078929695335 1105 74-75 1.0119839653001321 1105 76 0.9646874409464914 1106 1 -0.003611190601006342 1106 2 -0.11321614785712342 1106 3 0.10543860652439463 1106 4 0.08289864047958417 1106 5 0.05248010416548965 1106 6 -0.012192888216020492 1106 7 -0.2607743029439433 1106 8 -0.03958115951164842 1106 9 -0.26898521379849427 1106 10-11 -0.06100337912793208 1106 12-13 0.10351151940560044 1106 14-15 0.03361832091098904 1106 16-17 -0.14235210538495835 1106 18-19 0.05351672602895974 1106 20-21 -0.014341152717285865 1106 22-23 -0.10091615621068684 1106 24-25 -0.030006902644288402 1106 26-27 -0.33052258174348736 1106 28-29 -0.08066668567040836 1106 30-31 -0.0032539683472663228 1106 32-33 -0.1855831977556619 1106 34-35 -0.2024175409085771 1106 36-37 -0.09158435989823488 1106 38-39 -0.2267580525865469 1106 40-41 0.011533100063026325 1106 42-43 -0.12792026806358336 1106 44-45 -0.01617412466398349 1106 46-47 0.022007681784536715 1106 48-49 -0.1452629741792748 1106 50-51 -0.056541152315723764 1106 52-53 -0.1622932783663913 1106 54-55 -0.12984524275929488 1106 56-57 -0.006053836213702368 1106 58-59 0.0998218922922618 1106 60-61 0.028982261047730162 1106 62-63 0.08159915634725934 1106 64-65 -0.14062559127672358 1106 66-67 0.17131933446830772 1106 68-69 -0.04965222040957684 1106 70-71 0.09509875020154368 1106 72-73 0.1591490322100384 1106 74-75 0.29606228808493995 1106 76 0.4224406055034535 1107 1 0.06536090029421615 1107 2 -0.014352338188068359 1107 3 0.06033413801745269 1107 4 -0.013715664842969488 1107 5 0.030595095777506742 1107 6 0.10779338599474642 1107 7 0.010881942290154711 1107 8 -0.006410502198853862 1107 9 0.027263070477850704 1107 10-11 -0.046648491221880306 1107 12-13 -0.05284809627230658 1107 14-15 0.17304946320167147 1107 16-17 -0.003283171421252007 1107 18-19 -0.18317688385361208 1107 20-21 0.14802349575000306 1107 22-23 0.11753359214984016 1107 24-25 0.014735357535670346 1107 26-27 -0.10797416020924544 1107 28-29 0.08755048681700828 1107 30-31 -0.1939852478958386 1107 32-33 -0.035037978905577916 1107 34-35 -0.20058743567752657 1107 36-37 -0.16814376206384907 1107 38-39 -0.21697842775812148 1107 40-41 0.02924318089267075 1107 42-43 0.1678323627126872 1107 44-45 0.16706516159498364 1107 46-47 0.16481308059496058 1107 48-49 0.02722444384725975 1107 50-51 0.21906125732282788 1107 52-53 0.03084001429793659 1107 54-55 0.2443159590098105 1107 56-57 -0.06768644258857393 1107 58-59 0.047835465572724445 1107 60-61 -0.060254432562146576 1107 62-63 -0.08980163669833274 1107 64-65 0.26083468018888567 1107 66-67 0.49967071467266777 1107 68-69 0.41262092279615814 1107 70-71 0.03371397057671288 1107 72-73 0.07025548333098186 1107 74-75 0.31226634481819815 1107 76 -0.14359569158362717 1108 1 -0.15138767294611455 1108 2 -0.00258157090842559 1108 3 -0.03113700973143807 1108 4 -0.056716616016082355 1108 5 0.02928723274644085 1108 6 0.08278545955213445 1108 7 -0.015354582757403534 1108 8 0.14870997972885647 1108 9 -0.009991209801157197 1108 10-11 -0.14142892876150626 1108 12-13 -0.15246366380559806 1108 14-15 -0.2804223186626231 1108 16-17 -0.2665401466907511 1108 18-19 0.018769057807773493 1108 20-21 -0.07948522333688146 1108 22-23 0.05856085911242559 1108 24-25 0.02010552240568586 1108 26-27 -0.29159020332910046 1108 28-29 -0.07700246181963877 1108 30-31 -0.2125727558222863 1108 32-33 -0.28874359082694667 1108 34-35 -0.21049548894321646 1108 36-37 -0.14071827062441855 1108 38-39 -0.21955452160720057 1108 40-41 -0.21802219640598963 1108 42-43 -0.38907949524546837 1108 44-45 -0.2945510400537188 1108 46-47 -0.23188555605690198 1108 48-49 -0.24049115539180121 1108 50-51 -0.07134378389466889 1108 52-53 -0.09392219242369038 1108 54-55 -0.3380397797346433 1108 56-57 -0.13854883954481778 1108 58-59 -0.2881069884539045 1108 60-61 -0.3078764997783878 1108 62-63 -0.5012830888226247 1108 64-65 -0.07348285310217051 1108 66-67 -0.11407373681750244 1108 68-69 -0.1614318673116415 1108 70-71 -0.0865301638557554 1108 72-73 -0.3827605280830966 1108 74-75 -0.07822985048935038 1108 76 0.6903453356833325 1109 1 0.04501325584645599 1109 2 0.030893661289717045 1109 3 0.0710301729311027 1109 4 -0.08477389155788018 1109 5 0.03141571571857327 1109 6 -0.2134606704788311 1109 7 0.1444751386048253 1109 8 -0.030469586834612983 1109 9 0.010906623016182948 1109 10-11 -0.11192041482961912 1109 12-13 -0.046329762876801794 1109 14-15 -0.06989600287315767 1109 16-17 0.08553028674577945 1109 18-19 0.18546797421643646 1109 20-21 0.13287932774671418 1109 22-23 -0.13725957432410496 1109 24-25 -5.020627336378425E-4 1109 26-27 0.01897481215078045 1109 28-29 -0.05416964448217243 1109 30-31 0.06741950423963772 1109 32-33 -0.11372424098174605 1109 34-35 -0.05608372423733243 1109 36-37 0.09534984083069276 1109 38-39 -0.1698254194400164 1109 40-41 -0.17680702612735644 1109 42-43 -0.0701940463290498 1109 44-45 -0.04832425986796096 1109 46-47 0.01937218388117401 1109 48-49 0.08748872076918701 1109 50-51 0.031694141801921205 1109 52-53 0.30120164658560356 1109 54-55 -0.03773018221142621 1109 56-57 0.04372670225084363 1109 58-59 0.05854548832628126 1109 60-61 0.3337643670946733 1109 62-63 -0.06136435817246877 1109 64-65 -0.02578548468112274 1109 66-67 0.14095722293481572 1109 68-69 0.4623103927502825 1109 70-71 0.46223144605136923 1109 72-73 -0.06358483148866867 1109 74-75 0.31476550554781113 1109 76 0.41906050596197275 1110 1 -0.09006850965326407 1110 2 -0.3165997895319066 1110 3 -0.04362014063562469 1110 4 -0.07248922060448137 1110 5 -0.25419792244924366 1110 6 -0.2869007752656856 1110 7 -0.18961234254147996 1110 8 -0.053213097194223735 1110 9 0.09291027467927648 1110 10-11 -0.12612022430807457 1110 12-13 -0.10729665975701153 1110 14-15 -0.38248034835223876 1110 16-17 -0.17093454615093862 1110 18-19 0.04116278250412364 1110 20-21 -0.17011444061083125 1110 22-23 -0.18907746747326826 1110 24-25 -0.06554731160241545 1110 26-27 -0.05398898875420599 1110 28-29 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0.22903664698252157 2115 54-55 0.10435225805213832 2115 56-57 -0.0453470851588591 2115 58-59 0.047545845554189725 2115 60-61 0.04991702248882035 2115 62-63 -0.11519165832329747 2115 64-65 0.29304042625005877 2115 66-67 0.2330079096062505 2115 68-69 0.02248344982736228 2115 70-71 -0.04722517060338305 2115 72-73 0.5157296878413291 2115 74-75 0.24287338837704908 2115 76 0.47846949222854107 2116 1 0.18415907435692702 2116 2 0.045738958676409425 2116 3 0.005312810471245655 2116 4 0.07271702242785238 2116 5 0.06808667700926918 2116 6 0.07030180476298398 2116 7 -0.008612183280447994 2116 8 0.2162565635797904 2116 9 0.04975053586736067 2116 10-11 0.10040868156865912 2116 12-13 0.24841284812641362 2116 14-15 0.17642632468487562 2116 16-17 -0.13031302998594896 2116 18-19 0.025664693636471725 2116 20-21 -0.0146458150335107 2116 22-23 0.22830587382311762 2116 24-25 0.14198971658678516 2116 26-27 0.1751677240981664 2116 28-29 0.32676510928202873 2116 30-31 0.2252132853676514 2116 32-33 0.14572232940842866 2116 34-35 0.29673726685933133 2116 36-37 0.2701186041712731 2116 38-39 0.17667382098841955 2116 40-41 0.004211376657096366 2116 42-43 0.2251735286185479 2116 44-45 0.07020255589267066 2116 46-47 0.20785169467863085 2116 48-49 0.1411490865173164 2116 50-51 0.11892468619358709 2116 52-53 0.04611540156128058 2116 54-55 0.278991929974417 2116 56-57 0.1838195815078123 2116 58-59 0.06943428320349909 2116 60-61 0.3192075146053952 2116 62-63 0.3730528869864642 2116 64-65 0.20035050747318195 2116 66-67 0.16628749234234874 2116 68-69 0.00233692968084398 2116 70-71 -0.0435422593117849 2116 72-73 0.5104541662614537 2116 74-75 0.9665355957349107 2116 76 0.8037402359775427 2117 1 0.03380969547494317 2117 2 0.0788329027757868 2117 3 -0.196104114994597 2117 4 -0.12184819496345511 2117 5 0.11086618011486138 2117 6 0.05877230165739178 2117 7 0.18546778566365418 2117 8 -0.2825400202711421 2117 9 0.08441668493568244 2117 10-11 0.07904804492270046 2117 12-13 0.10279949408914746 2117 14-15 0.11900202344263988 2117 16-17 0.018328274361884667 2117 18-19 0.18274932096566232 2117 20-21 0.08753780297890756 2117 22-23 0.0991036222703201 2117 24-25 -0.007855003910108849 2117 26-27 0.08735716509195157 2117 28-29 -0.4229728565564841 2117 30-31 -0.03749051898017086 2117 32-33 0.016355093383580765 2117 34-35 0.22618214263572867 2117 36-37 0.1651205451650668 2117 38-39 0.049606662603324025 2117 40-41 -0.16473272272178718 2117 42-43 0.044884320544014145 2117 44-45 0.06416606520944157 2117 46-47 0.09771970710098543 2117 48-49 -0.20208655012864085 2117 50-51 0.040333847684273394 2117 52-53 0.21553504441841653 2117 54-55 0.14024969394957054 2117 56-57 -0.15368041849218628 2117 58-59 0.08360353786188313 2117 60-61 -0.14142913135733437 2117 62-63 0.07659520065106307 2117 64-65 0.12717504163467197 2117 66-67 0.012735473708815448 2117 68-69 0.10032800110941054 2117 70-71 -0.04113542701364281 2117 72-73 -0.3650796070304665 2117 74-75 -0.32707853469986503 2117 76 -0.41343755905351287 2118 1 -0.025995350396620154 2118 2 0.04581493641492784 2118 3 -0.06461520062151038 2118 4 0.04107229433318338 2118 5 0.1132744369955958 2118 6 0.12756052795708683 2118 7 0.053271684746221126 2118 8 0.08640111122732463 2118 9 0.13007799992039537 2118 10-11 0.17866100363828963 2118 12-13 0.07254337788118903 2118 14-15 0.06538485983407583 2118 16-17 0.059555338582065076 2118 18-19 -0.08264632123616877 2118 20-21 0.20478264242844801 2118 22-23 0.008464287407932147 2118 24-25 0.1064416321755175 2118 26-27 -0.1390572079967356 2118 28-29 0.1044115088869404 2118 30-31 0.0865681583898521 2118 32-33 0.19616205057012337 2118 34-35 0.17500668392013097 2118 36-37 0.18066431427821072 2118 38-39 0.21460570694583225 2118 40-41 0.08878409685008393 2118 42-43 0.11961195815868564 2118 44-45 0.12396155450607438 2118 46-47 0.20205839211627818 2118 48-49 0.07643504773068344 2118 50-51 0.01838239508489181 2118 52-53 0.05823859946428911 2118 54-55 0.23004327193122265 2118 56-57 0.016388388847261126 2118 58-59 0.06611500575179008 2118 60-61 0.16633080748059115 2118 62-63 0.2795242908651261 2118 64-65 0.2348776410230471 2118 66-67 0.22620068777608537 2118 68-69 0.08141076104825373 2118 70-71 0.024728487359439555 2118 72-73 -0.17631336085310068 2118 74-75 -0.11789466619833888 2118 76 -0.3407789199097806 2119 1 0.08719345343672558 2119 2 -0.0550245813006498 2119 3 -0.05778803219205031 2119 4 -0.013408704517594572 2119 5 4.362438091263243E-4 2119 6 0.11247453095676008 2119 7 0.005471766555373847 2119 8 0.023709979728856467 2119 9 0.17514120722867688 2119 10-11 -0.01214423214736371 2119 12-13 0.06725013103181965 2119 14-15 -0.2216727377038623 2119 16-17 0.020199293470156476 2119 18-19 0.13097782415037784 2119 20-21 0.04680332845661894 2119 22-23 0.10767249169707327 2119 24-25 -0.005466468878260855 2119 26-27 0.033774362544185976 2119 28-29 0.030062573379822766 2119 30-31 0.12884108930007443 2119 32-33 -0.12367277285845546 2119 34-35 -0.08960209303305788 2119 36-37 -0.06872698668207278 2119 38-39 -0.34926873548585036 2119 40-41 -0.11919132144789302 2119 42-43 -0.05753407117573772 2119 44-45 0.2016461607508404 2119 46-47 0.13470219117741777 2119 48-49 0.009487892546516719 2119 50-51 -0.16004433702909893 2119 52-53 -0.041919175326796676 2119 54-55 -0.017473235986734892 2119 56-57 0.1392335942466687 2119 58-59 0.012544620664435513 2119 60-61 0.25514730176368516 2119 62-63 -0.026519847119637063 2119 64-65 -0.19826285645450525 2119 66-67 -0.02972073648226825 2119 68-69 -0.06795424411351547 2119 70-71 -0.22640612765059132 2119 72-73 -0.6445879669030745 2119 74-75 -0.7192162735533714 2119 76 -1.9127807119197513 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 1.0 21 0.0 22 4.0 23 8.0 24 22.0 25 38.0 26 82.0 27 168.0 28 303.0 29 479.0 30 849.0 31 1324.0 32 2133.0 33 3605.0 34 6036.0 35 10608.0 36 17362.0 37 77252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.604775682596696 17.102331304665935 12.610994712162027 34.68189830057534 2 18.511590009644486 19.13515580830756 37.47381023645615 24.879443945591806 3 18.00192889687053 18.62798895872826 33.5179088097376 29.852173334663608 4 14.268016894476037 24.149456250623565 33.18450896271908 28.398017892181315 5 14.66959326881506 30.513153081246468 39.66709900562041 15.150154644318068 6 27.683827197445876 38.93877415278194 16.837939406032792 16.539459243739397 7 26.314476703581764 25.51714390235791 22.282915959958764 25.88546343410157 8 22.84412517875553 37.34909707672354 16.63756692939572 23.16921081512521 9 21.835611427051116 15.969104393228905 17.473145099604242 44.72213908011574 10-11 25.540423692174663 26.88857294888423 24.22178988326848 23.34921347567262 12-13 24.447104991852072 19.26652033655925 26.452908310884965 29.833466360703714 14-15 21.78156905783365 18.764757723901692 20.857860254747415 38.59581296351725 16-17 27.167514716152848 25.46684292793242 19.80860687086368 27.557035485051053 18-19 29.246898799427985 23.81605640360504 20.441734011772922 26.495310785194054 20-21 23.760766902790248 21.84475705876484 20.396005853204297 33.99847018524061 22-23 22.786341414746083 26.41549436296518 20.231384482357246 30.566779739931494 24-25 27.292227875885462 26.76178123648941 21.072782600019956 24.873208287605173 26-27 28.797515713858125 28.036765439489177 20.819614885762746 22.346103960889955 28-29 23.31263094881772 27.05028434600419 22.918537364062654 26.718547341115436 30-31 21.78364761049586 28.534370946822307 25.789434301107455 23.89254714157438 32-33 24.962170341547775 28.651601316970964 23.291845422195617 23.094382919285643 34-35 24.36978283281785 22.05967940403738 31.125494695533607 22.445043067611163 36-37 22.744354650969438 28.93386876849912 25.979829724965914 22.341946855565535 38-39 20.835827596527988 25.53917656057734 27.84637001563072 25.77862582726396 40-41 21.76909774186039 19.809854002461005 28.950497189796803 29.470551065881807 42-43 26.53937610163291 19.58370747281253 28.0380125710865 25.83890385446806 44-45 21.032458678373075 24.896903787954372 30.63911337257641 23.431524161096146 46-47 24.395972596361702 21.567893844158437 28.11866041438026 25.917473145099606 48-49 24.226778409657786 20.503674881106786 24.6994412850444 30.57010542419103 50-51 21.868036848581596 20.920216834613722 28.408410655492368 28.803335661312314 52-53 18.17694303102863 20.9318567295221 35.73364594765373 25.157554291795535 54-55 18.856214041038942 21.821892979480527 31.336259935481724 27.985633043998803 56-57 22.620057201769264 22.87655060028601 25.885047723569127 28.6183444743756 58-59 19.799876949682396 23.025374970900263 31.423143436762114 25.751604642655224 60-61 23.179603578436264 26.70940170940171 26.156090990721342 23.954903721440687 62-63 18.836259935481724 34.6893810901593 22.092520536100302 24.381838438258672 64-65 18.136619109381755 36.674814593102525 21.007100335894112 24.181465961621605 66-67 18.398101034287805 36.262014034387576 20.812963517243674 24.526921414080945 68-69 18.018557318168213 36.89056835943995 21.034121520502843 24.05675280188899 70-71 17.93499950114736 35.91697429246067 21.190428680701054 24.95759752569091 72-73 17.96160497522365 33.81056902457681 22.09418337823007 26.13364262196947 74-75 18.269230769230766 32.7209917190462 23.029532076224683 25.980245435498357 76 18.410156639728626 32.79374106222355 23.34547208088064 25.45063021716718 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 1.5 15 0.5 16 3.0 17 5.0 18 4.0 19 5.5 20 14.5 21 21.0 22 24.5 23 44.5 24 63.0 25 65.0 26 132.5 27 240.0 28 359.0 29 438.0 30 484.0 31 663.5 32 960.0 33 1123.0 34 1336.0 35 1943.0 36 4395.0 37 6453.0 38 7656.5 39 8549.0 40 7522.0 41 6294.0 42 5782.0 43 5745.5 44 5848.5 45 5956.5 46 5925.0 47 5847.5 48 5530.0 49 5092.0 50 4894.0 51 4517.0 52 3680.5 53 3083.5 54 2946.0 55 2845.5 56 2581.5 57 2361.0 58 2304.0 59 2203.5 60 2122.0 61 2185.0 62 2229.0 63 2272.0 64 2381.0 65 2441.0 66 2487.0 67 2539.0 68 2449.0 69 2188.5 70 1841.5 71 1665.0 72 1494.0 73 1104.0 74 716.5 75 548.0 76 385.0 77 150.5 78 50.5 79 22.0 80 14.0 81 3.5 82 1.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 120276.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.394592437393996 #Duplication Level Percentage of deduplicated Percentage of total 1 59.073788206236486 23.862615983238552 2 20.6133580323145 16.65336392962852 3 8.20417824431409 9.942133093884067 4 3.622517237830606 5.853204296784063 5 2.196151075434805 4.435631381156673 6 1.4531233919934137 3.521899630849047 7 1.1114541525162087 3.1427716252618976 8 0.7944838942060307 2.5674282483621003 9 0.6174745291756715 2.2448368751870698 >10 2.278481012658228 13.468189830057534 >50 0.016465987444684575 0.4514616382320662 >100 0.010291242152927859 0.9528085403571785 >500 0.0 0.0 >1k 0.006174745291756716 5.6777744520935185 >5k 0.002058248430585572 7.2258804749077115 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT 8691 7.2258804749077115 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 2491 2.0710698726262926 No Hit TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG 2285 1.8997971332601682 No Hit CACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTTCTG 2053 1.706907446207057 Illumina PCR Primer Index 8 (95% over 23bp) TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 474 0.39409358475506334 No Hit ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT 283 0.23529216136220027 RNA PCR Primer, Index 15 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT 146 0.12138747547307858 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC 125 0.1039276331105125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009977052778609198 0.0 2 0.0 0.0 0.0 0.0124713159732615 0.0 3 0.0 0.0 0.0 0.015797000232797898 0.0 4 0.0 0.0 0.0 0.054042369217466496 0.0 5 0.0 0.0 0.0 0.059862316671655195 0.0 6 0.0 0.0 0.0 0.059862316671655195 0.0 7 0.0 0.0 0.0 0.059862316671655195 0.0 8 0.0 0.0 0.0 0.06235657986630749 0.0 9 0.0 0.0 0.0 0.06734510625561209 0.0 10 0.0 0.0 0.0 0.0731650537098008 0.0 11 0.0 0.0 0.0 0.07565931690445309 0.0 12 0.0 0.0 0.0 0.0773221590342213 0.0 13 0.0 0.0 0.0 0.0773221590342213 0.0 14 0.0 0.0 0.0 0.07981642222887358 0.0 15 0.0 0.0 0.0 0.0806478432937577 0.0 16 0.0 0.0 0.0 0.08314210648840999 0.0 17 0.0 0.0 0.0 0.08563636968306229 0.0 18 0.0 0.0 0.0 0.09145631713725098 0.0 19 0.0 0.0 0.0 0.10226479098074429 0.0 20 0.0 0.0 0.0 0.10475905417539659 0.0 21 0.0 0.0 0.0 0.10559047524028069 0.0 22 0.0 0.0 0.0 0.11556752801888989 0.0 23 0.0 0.0 0.0 0.12388173866773089 0.0 24 0.0 0.0 0.0 0.1313645282516878 0.0 25 0.0 0.0 0.0 0.1396787389005288 0.0 26 0.0 0.0 0.0 0.15048721274402208 0.0 27 0.0 0.0 0.0 0.2602347933087233 0.0 28 0.0 0.0 0.0 0.37413947919784496 0.0 29 0.0 0.0 0.0 0.48804416508696663 0.0 30 0.0 0.0 0.0 0.5828261664837541 0.0 31 0.0 0.0 0.0 0.7308191160331238 0.0 32 0.0 0.0 0.0 0.8838005919717982 0.0 33 0.0 0.0 0.0 1.0700389105058365 0.0 34 0.0 0.0 0.0 1.3693504938641126 0.0 35 0.0 0.0 0.0 1.7609498154245236 0.0 36 0.0 0.0 0.0 2.320496192091523 0.0 37 0.0 0.0 0.0 3.0812464664604744 0.0 38 0.0 0.0 0.0 3.919318899863647 0.0 39 0.0 0.0 0.0 4.684226279557019 0.0 40 0.0 0.0 0.0 5.514815923376235 0.0 41 0.0 0.0 0.0 6.381988094050351 0.0 42 0.0 0.0 0.0 7.25581163324354 0.0 43 0.0 0.0 0.0 8.182014699524427 0.0 44 0.0 0.0 0.0 9.074129502145066 0.0 45 0.0 0.0 0.0 9.94878446240314 0.0 46 0.0 0.0 0.0 10.874987528684027 0.0 47 0.0 0.0 0.0 11.728856962319997 0.0 48 0.0 0.0 0.0 12.46549602580731 0.0 49 0.0 0.0 0.0 13.188832352256476 0.0 50 0.0 0.0 0.0 13.905517310186571 0.0 51 0.0 0.0 0.0 14.646313478998303 0.0 52 0.0 0.0 0.0 15.406232332302372 0.0 53 0.0 0.0 0.0 16.12790581662177 0.0 54 0.0 0.0 0.0 16.796368352788587 0.0 55 0.0 0.0 0.0 17.45069673085237 0.0 56 0.0 0.0 0.0 18.160730320263394 0.0 57 0.0 0.0 0.0 18.786790382121122 0.0 58 0.0 0.0 0.0 19.45026439189863 0.0 59 0.0 0.0 0.0 20.100435664638 0.0 60 0.0 0.0 0.0 20.699058831354552 0.0 61 0.0 0.0 0.0 21.269413681865043 0.0 62 0.0 0.0 0.0 21.893810901593003 0.0 63 0.0 0.0 0.0 22.494928331504205 0.0 64 0.0 0.0 0.0 23.094382919285643 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAATAC 30 1.01650585E-7 70.00001 2 CTTAATA 35 2.9534567E-7 60.0 1 TCTTTAT 90 0.0 58.33333 1 AAATTCT 25 2.3824078E-4 55.999996 52 CTTTATA 95 0.0 55.263157 2 CATATTC 20 0.0069369846 52.5 3 CTACTGG 20 0.0069369846 52.5 9 TTGTGCG 20 0.0069369846 52.5 21 TGAAGTT 20 0.0069369846 52.5 39 ACGTTAC 20 0.0069369846 52.5 69 ACGCACT 20 0.0069369846 52.5 8 GACGTTC 20 0.0069369846 52.5 22 GGCGTAA 20 0.0069369846 52.5 13 GGATATT 20 0.0069369846 52.5 28 CGTTATC 20 0.0069369846 52.5 10 CGTTACT 20 0.0069369846 52.5 70 TCTAACT 20 0.0069369846 52.5 21 TTCGGTC 20 0.0069369846 52.5 28 CGGTATA 20 0.0069369846 52.5 60 TTCTGAT 20 0.0069369846 52.5 19 >>END_MODULE