FastQCFastQC Report
Wed 25 May 2016
SRR1780247_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780247_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528955
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCAT207.836742E-444.0180839
ATCGTAC301.304246E-436.6470425
GCCTTTA250.002363258735.164531
CATCGTA353.2112552E-431.43553524
TTTGAGA300.005757168429.31763528
CTGAATA407.0216483E-427.5034911
GACTATC450.001393833124.45448938
GTTTTCA651.8184064E-523.67962830
ATCACTA500.002554059522.03615815
TCGTACT500.002586563121.98822626
CATCGTT602.8876038E-421.97991232
CATGTAC703.233667E-521.9799123
TAGATCT1301.6098056E-920.31603839
TTTATGA550.004437943520.03287119
CAAGAGT550.00446968820.00821939
ACATGAG708.15988E-418.8417059
CTACAGC708.15988E-418.8417054
CTTGTAC951.6069374E-518.50943
GCATATT600.007336882418.36346421
ATGGCTC600.007441797318.3183237
Produced by FastQC (version 0.11.5)