FastQCFastQC Report
Wed 25 May 2016
SRR1780247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780247_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528955
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGT174983.3080318741669896No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC52971.0014084373907044No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCG45720.8643457382953181No Hit
CACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCCGTCTTCTG32920.6223591798924294Illumina PCR Primer Index 1 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGTCACAATCTCGTATGCC8800.166365758901986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA900.036.667142
TCTTCTG4500.036.66367744
GTCTTCT4550.036.26077743
CCGTCTT5000.032.99730741
CGTCTTC5050.032.670642
ATGCCGT24850.032.2227244
TATGCCG28900.027.63096243
TCTTTAT1200.027.560281
GCCACTA407.030223E-427.49775528
GCCGTCT6000.027.49775540
GTCCAGA450.001379462824.4980261
GCTATAC450.001397070524.444763
TTATGTC450.001397840224.44244839
TATGTCA450.001397840224.44244840
TTAATAC651.8107101E-523.6926122
TGCCGTC7150.023.38270639
TTTATAC1600.023.3753013
AATCTCG34650.022.6013336
GTATGCC35650.022.58441242
GTCACAA35150.022.46758331
Produced by FastQC (version 0.11.5)