FastQCFastQC Report
Wed 25 May 2016
SRR1780246_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780246_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72161
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAATTG200.006925790652.550
TGCGGAG200.006925790652.55
TCAGCCG200.006925790652.53
GTGTCTT200.006925790652.542
GCAGGTT400.00240448735.047
CATAAGG501.6946759E-435.029
GAACATA450.004282007531.11110926
CTTCAGG500.007166365627.99999861
TATTATG500.007166365627.99999817
TAGATCT906.0711445E-627.22222139
ATAAGGG700.001215444225.030
AGATCTC1001.3752879E-524.49999840
AGGGTGT800.002643229421.87533
GTAGATC800.002643229421.87538
TCTCGGT800.002643229421.87543
ATCTCGG850.003756943620.58823442
TTACACA850.003756943620.5882344
GATCTCG900.00523002219.44444341
CTTATCC1107.384673E-419.0909081
TCTTTCT950.00714692118.4210511
Produced by FastQC (version 0.11.5)