##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780246_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72161 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.21087568076939 34.0 27.0 34.0 12.0 34.0 2 29.348165906791756 34.0 27.0 34.0 12.0 34.0 3 29.07892074666371 34.0 27.0 34.0 11.0 34.0 4 28.974085724976096 34.0 27.0 34.0 11.0 34.0 5 28.97177145549535 34.0 27.0 34.0 11.0 34.0 6 31.68974931056942 37.0 27.0 38.0 11.0 38.0 7 32.11107107717465 37.0 31.0 38.0 12.0 38.0 8 32.172863458100636 37.0 31.0 38.0 11.0 38.0 9 32.33707958592591 37.0 31.0 38.0 12.0 38.0 10-11 32.01378861157689 37.0 30.0 38.0 11.0 38.0 12-13 32.088551987915906 37.0 31.0 38.0 11.0 38.0 14-15 31.911080777705408 37.0 31.0 38.0 11.0 38.0 16-17 31.988241570931663 37.0 31.0 38.0 11.0 38.0 18-19 32.04884217236457 37.0 31.0 38.0 11.0 38.0 20-21 32.02591427502391 37.0 31.0 38.0 11.0 38.0 22-23 32.04005626307839 37.0 31.0 38.0 11.0 38.0 24-25 32.03558708997935 37.0 31.0 38.0 11.0 38.0 26-27 31.990521195659703 37.0 31.0 38.0 11.0 38.0 28-29 32.04959049902302 38.0 31.0 38.0 11.0 38.0 30-31 32.05861892157814 38.0 31.0 38.0 11.0 38.0 32-33 32.127180887182824 38.0 31.0 38.0 11.0 38.0 34-35 32.130499854492044 38.0 31.0 38.0 11.0 38.0 36-37 32.04513518382505 37.0 31.0 38.0 11.0 38.0 38-39 32.04510053907235 37.5 31.0 38.0 11.0 38.0 40-41 32.10349080528263 37.5 31.0 38.0 11.0 38.0 42-43 32.16831806654564 37.5 31.0 38.0 11.0 38.0 44-45 32.26266958606449 38.0 31.0 38.0 11.0 38.0 46-47 32.140512188024005 37.5 31.0 38.0 11.0 38.0 48-49 32.09921564279874 37.0 31.0 38.0 11.0 38.0 50-51 32.01719765524314 37.0 31.0 38.0 11.0 38.0 52-53 32.1130319701778 37.0 31.0 38.0 11.0 38.0 54-55 32.09873754521141 37.0 31.0 38.0 11.0 38.0 56-57 32.01445379082884 37.0 31.0 38.0 11.0 38.0 58-59 32.052057205415664 37.0 31.0 38.0 11.0 38.0 60-61 32.12188024001885 37.0 31.0 38.0 11.0 38.0 62-63 31.944700045731075 37.0 30.5 38.0 11.0 38.0 64-65 31.893696040797664 37.0 30.0 38.0 11.0 38.0 66-67 31.784980806807 37.0 28.5 38.0 11.0 38.0 68-69 31.720098113939663 37.0 28.0 38.0 11.0 38.0 70-71 31.608978534111223 37.0 27.0 38.0 11.0 38.0 72-73 31.561300425437565 37.0 27.0 38.0 11.0 38.0 74-75 31.561134130624573 37.0 27.0 38.0 11.0 38.0 76 30.061127201674033 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.27832250348179954 1101 2 -0.17737190691125093 1101 3 -0.6329985229256287 1101 4 0.005758269678288741 1101 5 -0.18832637118672935 1101 6 0.21379591007838883 1101 7 -0.9468339720826826 1101 8 -1.2075108542823862 1101 9 -1.473218731938207 1101 10-11 -1.2317530473148892 1101 12-13 -0.4869223220858778 1101 14-15 -1.429247118150947 1101 16-17 -0.7160320855721345 1101 18-19 -0.993017964311619 1101 20-21 -1.039915841198514 1101 22-23 -0.9989477519887693 1101 24-25 -1.1837051050408292 1101 26-27 -1.2785742049274482 1101 28-29 -1.4745824305616821 1101 30-31 -1.3436623501475395 1101 32-33 -1.6720036303810701 1101 34-35 -1.0285723078367788 1101 36-37 -0.5793417803527703 1101 38-39 -1.3699674931947285 1101 40-41 -0.7641095630057322 1101 42-43 -1.5203034391397878 1101 44-45 -1.2878478621791203 1101 46-47 -1.2335459102959554 1101 48-49 -1.2426010135832044 1101 50-51 -1.2350228541062407 1101 52-53 -0.8963208518371317 1101 54-55 -0.9679855524831567 1101 56-57 -1.354620092788462 1101 58-59 -1.3785298750982093 1101 60-61 -1.7881600396720323 1101 62-63 -1.1449655002612324 1101 64-65 -1.3163437110640466 1101 66-67 -1.200638285261281 1101 68-69 -1.0954555046249617 1101 70-71 -1.2608236773908956 1101 72-73 -1.8601207286364847 1101 74-75 -1.8215536662805256 1101 76 -2.190439919078269 1102 1 -0.07256848112019654 1102 2 -0.27415401762845804 1102 3 0.39566250788974955 1102 4 0.3189297995392124 1102 5 0.2356169066970999 1102 6 -0.22160087203233303 1102 7 -0.4419253274140189 1102 8 -0.008492583216117566 1102 9 -0.2936168775613339 1102 10-11 0.2167743592845106 1102 12-13 -0.05933846607279136 1102 14-15 -0.4185571808725186 1102 16-17 0.008224800165521629 1102 18-19 -0.5288783406410431 1102 20-21 -0.9328801171734256 1102 22-23 -0.819823126954681 1102 24-25 -0.533500578179634 1102 26-27 -1.1270607061546087 1102 28-29 -0.7007073282982503 1102 30-31 -0.5308316983886101 1102 32-33 -0.673735291139181 1102 34-35 -0.04628433401621379 1102 36-37 -0.5627341992237831 1102 38-39 -0.5329617664426181 1102 40-41 -0.7158821291360837 1102 42-43 -0.9107043117877254 1102 44-45 -0.6266125199375061 1102 46-47 -1.0240422288124549 1102 48-49 -0.20255465416133944 1102 50-51 -0.7633702770677928 1102 52-53 -0.6467299055595248 1102 54-55 -0.6389972786898639 1102 56-57 -0.5438074393933192 1102 58-59 -1.1014777889242247 1102 60-61 -1.0976091806592159 1102 62-63 -0.2617230677550957 1102 64-65 -0.7809332938292464 1102 66-67 -0.9585466572274655 1102 68-69 -0.6336474871720021 1102 70-71 -0.6881757362946317 1102 72-73 -0.515670224136894 1102 74-75 -1.0687584658442013 1102 76 -0.5328178847177583 1103 1 0.4082803673315958 1103 2 0.7589917294523865 1103 3 0.6048005201365712 1103 4 0.516763054367285 1103 5 0.5255492268993933 1103 6 0.6291069148630726 1103 7 0.4670416260034429 1103 8 0.8479915380621108 1103 9 0.5708004068177743 1103 10-11 -0.09419323870244867 1103 12-13 0.7769532760002456 1103 14-15 0.5463509679734528 1103 16-17 0.42966169433216805 1103 18-19 0.5428672031524968 1103 20-21 0.49596932626560175 1103 22-23 0.7544015303078808 1103 24-25 0.6182087705572599 1103 26-27 0.5501339290438452 1103 28-29 0.5474271388163103 1103 30-31 0.15418453980461067 1103 32-33 0.8545513935423728 1103 34-35 0.5568343907278148 1103 36-37 0.3622850139055984 1103 38-39 0.4582621718770383 1103 40-41 0.7571823030229723 1103 42-43 0.8447683311951408 1103 44-45 0.03392247274911142 1103 46-47 0.5379851901825177 1103 48-49 0.6686430055555519 1103 50-51 0.02980968177892862 1103 52-53 0.5091815405073703 1103 54-55 0.4162249738326338 1103 56-57 0.7303081368766087 1103 58-59 0.6269725172462941 1103 60-61 0.8924619698973473 1103 62-63 0.8428335428009639 1103 64-65 0.9441826047254303 1103 66-67 0.6330937721549859 1103 68-69 0.6899511939396312 1103 70-71 0.35544426519283334 1103 72-73 0.5020802283013133 1103 74-75 0.42964250596827824 1103 76 0.4913782627399108 1104 1 0.4248657556275113 1104 2 1.043427688230448 1104 3 0.7236816390176912 1104 4 1.907934383038615 1104 5 0.6670886085703565 1104 6 0.4436542096735252 1104 7 0.503570886216913 1104 8 0.1697158663653866 1104 9 0.33918005348689206 1104 10-11 0.6643754951952339 1104 12-13 0.0017416455254597452 1104 14-15 0.565363205735693 1104 16-17 0.09978269175579868 1104 18-19 0.5188747482389857 1104 20-21 0.628100353133469 1104 22-23 0.16415953546062667 1104 24-25 0.6212797127729317 1104 26-27 0.9880249853559562 1104 28-29 0.574202994539533 1104 30-31 1.078250605318047 1104 32-33 0.964990296744439 1104 34-35 0.7574347771834624 1104 36-37 0.25590619535572756 1104 38-39 1.1649009278579001 1104 40-41 0.6101566313262481 1104 42-43 0.5201570871760026 1104 44-45 1.0521065912621737 1104 46-47 0.5818640087323885 1104 48-49 -0.1590736856479893 1104 50-51 0.42446601229052305 1104 52-53 0.7264018202276503 1104 54-55 1.0462857025775705 1104 56-57 0.5680772756325894 1104 58-59 0.6086158738896472 1104 60-61 0.9794259927166351 1104 62-63 0.7939515037873512 1104 64-65 0.7029123618104443 1104 66-67 0.6062949131192923 1104 68-69 0.8481679771564181 1104 70-71 0.9104566869815791 1104 72-73 0.8820604454525842 1104 74-75 0.980065766917857 1104 76 0.5428596760634292 1105 1 -0.3570143788011677 1105 2 -0.6464061429868764 1105 3 -0.6321809450002327 1105 4 -1.0264849597545158 1105 5 -1.4631858499808033 1105 6 -1.102555648429533 1105 7 -0.9028891585926502 1105 8 -1.6968823412522376 1105 9 -1.8735253236602318 1105 10-11 -2.128917073886175 1105 12-13 -2.6756295269913473 1105 14-15 -1.4761556516205445 1105 16-17 -1.6593892649282935 1105 18-19 -1.5176730486243422 1105 20-21 -1.2736920292414347 1105 22-23 -1.4752635414624535 1105 24-25 -1.984139443313694 1105 26-27 -1.43654558970006 1105 28-29 -1.8714654147211078 1105 30-31 -0.9083532034944994 1105 32-33 -1.1677113462727426 1105 34-35 -1.4034828852511865 1105 36-37 -1.234221698594979 1105 38-39 -1.0614340235984088 1105 40-41 -1.3338080811457438 1105 42-43 -1.7817239807851628 1105 44-45 -1.459539226512284 1105 46-47 -1.0960906215887505 1105 48-49 -0.8434696901289414 1105 50-51 -0.8451148316228512 1105 52-53 -1.0998211073302357 1105 54-55 -1.3014551488040595 1105 56-57 -1.1009154428140135 1105 58-59 -1.355816538358301 1105 60-61 -1.2664431260287046 1105 62-63 -1.2130033132402822 1105 64-65 -1.070635995172374 1105 66-67 -0.8995396649240277 1105 68-69 -0.7968096180639002 1105 70-71 -1.3978190785150666 1105 72-73 -1.3444845508132879 1105 74-75 -1.1821821793106722 1105 76 -1.6876294949597224 1106 1 -0.2663607809937609 1106 2 0.8307620643806146 1106 3 0.705278989036092 1106 4 0.5119783653720695 1106 5 0.2528219541721235 1106 6 0.4377193550544618 1106 7 -0.1741066993554128 1106 8 0.08722598782287605 1106 9 0.6186472967699288 1106 10-11 0.0038928856994644434 1106 12-13 0.5688193047083807 1106 14-15 -0.25747678322272094 1106 16-17 0.8842071488776213 1106 18-19 0.8921494998032102 1106 20-21 0.22802674253354382 1106 22-23 -0.20971330222800333 1106 24-25 -0.06600175575853484 1106 26-27 -0.04555985086046377 1106 28-29 -0.1798455884564163 1106 30-31 -0.7309829243102186 1106 32-33 0.12249397727443068 1106 34-35 0.20276740508187885 1106 36-37 0.2163519995515415 1106 38-39 -0.34317323482152773 1106 40-41 0.1040722551760851 1106 42-43 -0.04518382191490389 1106 44-45 0.07937701820365106 1106 46-47 0.5212387786992636 1106 48-49 -0.24044790353536172 1106 50-51 -0.11133864357992707 1106 52-53 0.016358574000186366 1106 54-55 -0.11009081564105117 1106 56-57 -0.3199310975731535 1106 58-59 -0.021352841605384754 1106 60-61 -0.28217917842801654 1106 62-63 -0.3294152610267851 1106 64-65 0.032699351136908916 1106 66-67 0.042184681245895916 1106 68-69 -0.27655598309386065 1106 70-71 0.7071189063411616 1106 72-73 0.32983142447356073 1106 74-75 0.3219870035759307 1106 76 -0.2024016415663077 1107 1 -0.43953865064582587 1107 2 -1.4231051721130896 1107 3 0.37267342393180414 1107 4 -0.3226014646088835 1107 5 -0.6961955587186672 1107 6 -1.2676041921188563 1107 7 -0.49512216830138556 1107 8 -0.42503768105806117 1107 9 -0.08711755666993781 1107 10-11 -0.5755190156337413 1107 12-13 -0.4668916629136781 1107 14-15 -0.9688740982224857 1107 16-17 -0.47249606104479724 1107 18-19 -0.37204198066317673 1107 20-21 -0.6426240680763868 1107 22-23 -0.45827325020032106 1107 24-25 -0.4647219675855432 1107 26-27 -0.14528345377772922 1107 28-29 -0.038941142876449675 1107 30-31 -0.1923337860187715 1107 32-33 -0.1822744530688567 1107 34-35 -0.7044537590375981 1107 36-37 -0.4866488830609974 1107 38-39 -0.21677382942365142 1107 40-41 -0.7197304112428284 1107 42-43 -0.5462870875812769 1107 44-45 -0.100315925541409 1107 46-47 -0.40919741770525775 1107 48-49 -0.9939551270221436 1107 50-51 -1.0756973558946932 1107 52-53 -0.7527337286894564 1107 54-55 -0.34029010026036843 1107 56-57 -0.23198340397906136 1107 58-59 -0.5524184801058745 1107 60-61 -0.8077052619510319 1107 62-63 -0.4775664200363998 1107 64-65 -0.3376262864345101 1107 66-67 -0.5742969464774248 1107 68-69 -0.45700379282117964 1107 70-71 -0.41723655424322104 1107 72-73 -0.3590476576094055 1107 74-75 -0.7622792666893154 1107 76 0.0647976895062996 1108 1 -0.34451070124894656 1108 2 -0.6309604236576618 1108 3 -0.7588358434997922 1108 4 -0.5640450264407946 1108 5 -0.10656135257960386 1108 6 0.011350402369721735 1108 7 -0.4946808620348371 1108 8 -0.35854753689801555 1108 9 -0.797620645813808 1108 10-11 0.7194697065394458 1108 12-13 0.20026534404915353 1108 14-15 -0.047083375460886145 1108 16-17 -0.2585360728129622 1108 18-19 0.20501499687924252 1108 20-21 -0.8483953733622549 1108 22-23 -0.6141524303513428 1108 24-25 -1.0937795335363134 1108 26-27 -0.058983561652595995 1108 28-29 -0.45376903343249353 1108 30-31 -0.41014294557550457 1108 32-33 -0.3212859270317878 1108 34-35 -0.05693488093618981 1108 36-37 -0.6195065863070504 1108 38-39 -0.6008819004249304 1108 40-41 -0.4948113173917008 1108 42-43 -0.07801210474319475 1108 44-45 -0.24837417796859285 1108 46-47 0.46169598231436026 1108 48-49 -0.6006286584529548 1108 50-51 -0.49052524645073703 1108 52-53 -1.420111388785564 1108 54-55 -1.1414571101652413 1108 56-57 -0.7807232294179158 1108 58-59 -0.48413859386482017 1108 60-61 -0.3096911401505018 1108 62-63 -0.5023286049927584 1108 64-65 -0.6670614144133289 1108 66-67 -0.7068849625605864 1108 68-69 -0.49186698787854866 1108 70-71 -0.30431118403629753 1108 72-73 -0.11966352502139088 1108 74-75 -0.553345266493178 1108 76 -0.504634496430743 1109 1 0.9534690967308528 1109 2 -0.23702102971826733 1109 3 -0.056815641479591505 1109 4 0.3298943674985999 1109 5 -0.02575327177152431 1109 6 0.5517544427737633 1109 7 1.0103696430156646 1109 8 0.707109028758552 1109 9 1.2074298592366972 1109 10-11 1.330580817650553 1109 12-13 -0.49922960544579453 1109 14-15 0.2609974326199165 1109 16-17 0.21621140193451538 1109 18-19 0.7252170170812562 1109 20-21 0.6920086670417831 1109 22-23 0.9262516100527023 1109 24-25 0.4617760220192437 1109 26-27 0.21879421612518257 1109 28-29 0.1851198554563922 1109 30-31 0.4130893776568172 1109 32-33 0.5095221537762953 1109 34-35 0.8344792604779485 1109 36-37 0.5724126056121435 1109 38-39 0.6945726450296164 1109 40-41 0.44710787452748946 1109 42-43 0.5507757740446877 1109 44-45 0.33495915536474286 1109 46-47 0.24452426514264403 1109 48-49 0.38927033144603485 1109 50-51 0.8503838444583494 1109 52-53 0.7819088132346366 1109 54-55 -4.296875109588427E-5 1109 56-57 0.32028687159218805 1109 58-59 0.4603058505796227 1109 60-61 0.6751573446979862 1109 62-63 0.7426208899567364 1109 64-65 0.12586787851596526 1109 66-67 0.9118019061262785 1109 68-69 0.6571229111113546 1109 70-71 0.4936686139435018 1109 72-73 0.5848819295240624 1109 74-75 1.1486749355270298 1109 76 -0.05513954693579137 1110 1 0.13640421716597473 1110 2 -0.14407231176955193 1110 3 0.0025277928638445246 1110 4 -0.6641810015767717 1110 5 -0.8617575452757968 1110 6 -0.3207688672495479 1110 7 -0.16950603686001386 1110 8 -0.43605336440384335 1110 9 0.33437236117919866 1110 10-11 -0.7154321971124595 1110 12-13 0.32145318398699985 1110 14-15 -0.2855983327258471 1110 16-17 -0.316082692859581 1110 18-19 -0.23352909791485388 1110 20-21 -0.4944958007126843 1110 22-23 -0.7805520030009134 1110 24-25 -0.2609318256886013 1110 26-27 0.05773652381748917 1110 28-29 -0.1565662362296969 1110 30-31 -0.5539609331434932 1110 32-33 -0.0037597032555609644 1110 34-35 -0.17766137666269088 1110 36-37 -0.44842072772119224 1110 38-39 -0.6884644567574831 1110 40-41 -0.2768625994429783 1110 42-43 -0.15291983590092073 1110 44-45 0.10018351433910055 1110 46-47 -0.10082829895992518 1110 48-49 -0.324939070263369 1110 50-51 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-0.11674047248633457 2116 34-35 0.26698225654026686 2116 36-37 -0.6634631006025486 2116 38-39 -0.16384200999520715 2116 40-41 -0.28294691061203636 2116 42-43 -0.4948499749708901 2116 44-45 -0.17775270904203921 2116 46-47 -0.0012384467217643191 2116 48-49 0.28286731826336364 2116 50-51 0.25202739864427315 2116 52-53 -0.7489078277514949 2116 54-55 0.1997515869756583 2116 56-57 0.386756654163662 2116 58-59 0.5694481207388407 2116 60-61 0.5525327941073286 2116 62-63 0.10351799661655647 2116 64-65 -0.202115343535052 2116 66-67 0.017990914857662688 2116 68-69 -0.3180097716484518 2116 70-71 -0.20013381714706568 2116 72-73 -0.6609235832305984 2116 74-75 -0.4115031161764513 2116 76 -0.34104949386268757 2117 1 0.23232912559088348 2117 2 0.0010742083769699207 2117 3 0.07395636429241748 2117 4 -0.44806378545955283 2117 5 -0.3789278749461289 2117 6 0.16160292762224415 2117 7 0.9225269301729568 2117 8 0.9174264890760142 2117 9 0.6168815186883592 2117 10-11 0.46577201534175217 2117 12-13 0.5150200338038466 2117 14-15 0.8477578943803792 2117 16-17 0.8272323254554337 2117 18-19 0.9112740156382557 2117 20-21 0.3460490710821915 2117 22-23 0.32533169663278727 2117 24-25 0.9110004087436323 2117 26-27 1.0967704066013724 2117 28-29 0.23995391029044555 2117 30-31 0.47820481777225865 2117 32-33 0.40409286084699403 2117 34-35 0.6533825793812653 2117 36-37 0.43848403418356696 2117 38-39 0.6708533088102797 2117 40-41 0.5230458254654451 2117 42-43 1.2656928014617108 2117 44-45 1.0313877267933051 2117 46-47 0.5927385508569287 2117 48-49 0.6491909663666675 2117 50-51 1.1912929353674429 2117 52-53 0.9553395491653731 2117 54-55 1.2415695853614537 2117 56-57 0.35825584706115876 2117 58-59 1.120749573523348 2117 60-61 0.8098976044382411 2117 62-63 0.6620833719192198 2117 64-65 0.7861312262793128 2117 66-67 0.7697564515808253 2117 68-69 0.6588364753249145 2117 70-71 0.7194081521830391 2117 72-73 -0.6707097010675653 2117 74-75 0.01600899036107606 2117 76 -0.5024699942662387 2118 1 0.4868214885738098 2118 2 -0.08293297940380384 2118 3 0.7140057802852269 2118 4 0.8135040492214891 2118 5 1.009886141226378 2118 6 0.1466658207040048 2118 7 0.46015047015498567 2118 8 0.5519727604147349 2118 9 0.10003515750237568 2118 10-11 -0.6752320277849329 2118 12-13 -0.18657475505509424 2118 14-15 0.3559564568242308 2118 16-17 -0.3800340716694528 2118 18-19 -0.5830543760957951 2118 20-21 0.053596240893512004 2118 22-23 0.8643591000469826 2118 24-25 0.6524087645387766 2118 26-27 0.43734767524468054 2118 28-29 -0.32383764406714377 2118 30-31 0.08366303879651582 2118 32-33 0.056215502356430136 2118 34-35 0.4262936305942091 2118 36-37 0.41675222825364955 2118 38-39 0.5497279718830583 2118 40-41 0.3102201291491866 2118 42-43 0.37648570352820343 2118 44-45 0.16043085347794772 2118 46-47 -0.06365183548629005 2118 48-49 0.03182798343765114 2118 50-51 -0.4140529765822407 2118 52-53 0.3934010945382411 2118 54-55 -0.2307462277562493 2118 56-57 -0.022528070279363988 2118 58-59 -0.5517010104838427 2118 60-61 0.12784459455504305 2118 62-63 -0.5085702668947967 2118 64-65 0.0717893574563142 2118 66-67 0.3927910427757908 2118 68-69 0.15821877541476326 2118 70-71 -0.16575581906583992 2118 72-73 -0.45833324104959416 2118 74-75 0.004497310217320916 2118 76 0.6323008952216291 2119 1 0.03088802283398806 2119 2 0.49104914572032854 2119 3 -0.5764195755572068 2119 4 0.16628458546776415 2119 5 0.09851573407642888 2119 6 -0.15857251097424552 2119 7 0.8360607647594236 2119 8 0.041173356243930925 2119 9 0.9517812680617936 2119 10-11 0.31758905794826475 2119 12-13 0.6459724147562262 2119 14-15 0.2595686713227394 2119 16-17 0.3997573881120786 2119 18-19 0.12540308831996327 2119 20-21 -0.22083689383009286 2119 22-23 -0.11013196251508361 2119 24-25 0.32813700022232695 2119 26-27 0.3240573740199224 2119 28-29 0.22147020101726156 2119 30-31 -0.05813603435806769 2119 32-33 -0.07726678827580713 2119 34-35 0.039190850057956084 2119 36-37 0.16671085122617768 2119 38-39 -0.06865170372104856 2119 40-41 0.12141675278374464 2119 42-43 -0.19530343913978498 2119 44-45 -0.5641636516528052 2119 46-47 -0.17762485766437663 2119 48-49 -0.14325890832004973 2119 50-51 0.21102977747270302 2119 52-53 -0.3436892728897618 2119 54-55 -0.05088028932526356 2119 56-57 -0.25593588226215047 2119 58-59 -0.31800355930873536 2119 60-61 0.19539259190691638 2119 62-63 -0.1541760265770229 2119 64-65 -0.1512121321166795 2119 66-67 -0.9341909168402296 2119 68-69 -1.3671660309407478 2119 70-71 -0.7285868352856362 2119 72-73 -0.26406809705753886 2119 74-75 -0.6176062978594707 2119 76 -0.6575451822361629 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 14.0 13 200.0 14 826.0 15 1409.0 16 1963.0 17 2305.0 18 2371.0 19 2057.0 20 1600.0 21 1237.0 22 919.0 23 799.0 24 657.0 25 733.0 26 717.0 27 825.0 28 922.0 29 1050.0 30 1280.0 31 1514.0 32 1901.0 33 2527.0 34 3329.0 35 4930.0 36 8969.0 37 27106.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.753384792339354 20.139687642909603 11.777830129848534 32.32909743490251 2 18.755283324787626 19.64911794459611 34.97041338118929 26.625185349426978 3 17.4373969318607 21.224761297653856 32.20853369548648 29.12930807499896 4 13.702692590180291 21.92735688252657 31.359044359141365 33.01090616815177 5 15.536092903368855 32.07965521542107 32.836296614514765 19.547955266695308 6 26.85799808760965 31.691633985116614 17.929352420282424 23.52101550699131 7 25.523482213383964 29.280359196796052 20.587297847867962 24.608860741952025 8 22.416540790731837 34.32324940064578 19.033827136541902 24.226382672080486 9 22.405454469865994 18.681836449051424 20.24777927135156 38.66492980973102 10-11 24.26864927038151 24.356646942254127 25.351644239963417 26.02305954740095 12-13 23.540416568506533 21.583680935685482 25.172877316001717 29.703025179806264 14-15 23.30898962043209 22.663211429996814 23.288895663862753 30.738903285708346 16-17 23.956153600975597 23.979019137761394 24.03237205692826 28.03245520433475 18-19 22.872465736339574 23.029752913623703 24.941450367927274 29.156330982109452 20-21 23.03183159878605 23.39075123681767 25.10913097102313 28.46828619337315 22-23 24.888790343814527 23.184961405745486 23.79193747315032 28.134310777289674 24-25 23.74343481936226 24.726652901151592 23.123986640983357 28.405925638502794 26-27 22.71864303432602 25.025983564529312 24.906112720167403 27.34926068097726 28-29 23.447568631255113 23.825889330801957 26.421474203517135 26.3050678344258 30-31 23.380357811005943 23.33947700281315 26.264187026233003 27.015978159947895 32-33 22.16432699103394 24.622718643034325 25.99880822050692 27.214146145424813 34-35 22.400604204487188 24.26864927038151 26.32308310583279 27.00766341929851 36-37 23.10042820914344 22.993722370809717 26.56421058466485 27.341638835381993 38-39 21.574673299981985 22.98748631532268 28.946383780712576 26.491456603982762 40-41 21.339088981582847 22.44287080278821 29.006665650420587 27.211374565208352 42-43 20.18403292637297 22.639652998156897 29.047546458613375 28.128767616856752 44-45 19.121824808414516 22.515624783470294 30.560829256800766 27.801721151314425 46-47 19.353251756488962 23.53348761796538 30.279513864830033 26.83374676071562 48-49 19.09895927162872 23.606934493701583 29.94484555369244 27.34926068097726 50-51 19.00264685910672 24.937985892656698 28.417011959368633 27.642355288867947 52-53 17.93974584609415 24.75090422804562 28.775931597400255 28.53341832845997 54-55 18.222447028173114 24.279042696193233 28.66437549368773 28.834134781945924 56-57 18.755976219841745 24.550657557406357 27.816271947450836 28.87709427530106 58-59 19.071243469464115 24.29636507254611 27.52872049999307 29.1036709579967 60-61 18.715095411648953 23.540416568506533 27.84329485456133 29.901193165283185 62-63 19.102423746899294 23.694932165574205 27.434486772633417 29.768157314893084 64-65 19.98447915078782 23.615249234350966 26.032760078158564 30.36751153670265 66-67 19.484208921716718 24.83751610981001 25.009354083230555 30.668920885242724 68-69 19.953298873352644 25.04399883593631 24.66914261166004 30.33355967905101 70-71 20.732112914178018 24.61093942711437 25.090422804562024 29.56652485414559 72-73 20.886628511245686 23.50577181580078 26.554510053907237 29.0530896190463 74-75 21.191502335056335 23.272959077618104 26.769307520682915 28.766231066642646 76 20.8505979684317 22.212829644821998 27.369354637546593 29.567217749199703 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 2.0 13 1.0 14 2.0 15 4.0 16 4.0 17 4.0 18 5.0 19 9.0 20 12.5 21 13.0 22 29.0 23 54.0 24 74.5 25 86.0 26 121.5 27 193.0 28 288.5 29 348.0 30 400.5 31 545.0 32 782.0 33 927.0 34 1020.0 35 1352.0 36 1668.5 37 1746.0 38 1988.5 39 2463.0 40 2996.5 41 3474.5 42 3651.0 43 3712.5 44 3962.0 45 4133.0 46 4116.0 47 4125.0 48 3986.0 49 3566.5 50 3295.0 51 3209.5 52 2776.5 53 2311.0 54 2193.0 55 2123.0 56 1947.5 57 1805.0 58 1768.0 59 1740.0 60 1802.0 61 1936.0 62 1980.0 63 1980.5 64 1995.0 65 1912.5 66 1711.5 67 1607.0 68 1408.5 69 1038.0 70 685.0 71 504.0 72 386.5 73 198.0 74 84.0 75 41.0 76 32.5 77 15.0 78 6.0 79 6.0 80 8.0 81 7.5 82 5.0 83 5.0 84 3.5 85 3.5 86 4.0 87 3.0 88 3.0 89 2.5 90 3.0 91 4.5 92 5.0 93 4.5 94 2.0 95 2.0 96 4.0 97 3.0 98 2.5 99 12.5 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 72161.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.74181344493563 #Duplication Level Percentage of deduplicated Percentage of total 1 80.19902836545995 56.734247030944694 2 11.08956276445698 15.68991560538241 3 4.055007052186178 8.605756572109588 4 1.9138849710076793 5.415667742963651 5 0.9912239460899546 3.5060489738224248 6 0.5680927754270491 2.411274788320561 7 0.42117223005798465 2.085614112886462 8 0.24486757561510736 1.3857901082302073 9 0.1488794859739853 0.947880434029462 >10 0.36828083372512144 3.2178046313105417 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004157370324690622 0.0 2 0.0 0.0 0.0 0.004157370324690622 0.0 3 0.0 0.0 0.0 0.004157370324690622 0.0 4 0.0 0.0 0.0 0.01662948129876249 0.0 5 0.0 0.0 0.0 0.019401061515222903 0.0 6 0.0 0.0 0.0 0.022172641731683318 0.0 7 0.0 0.0 0.0 0.022172641731683318 0.0 8 0.0 0.0 0.0 0.023558431839913525 0.0 9 0.0 0.0 0.0 0.027715802164604147 0.0 10 0.0 0.0 0.0 0.03603054281398539 0.0 11 0.0 0.0 0.0 0.0374163329222156 0.0 12 0.0 0.0 0.0 0.0374163329222156 0.0 13 0.0 0.0 0.0 0.038802123030445806 0.0 14 0.0 0.0 0.0 0.04157370324690622 0.0 15 0.0 0.0 0.0 0.04711686367982705 0.0 16 0.0 0.0 0.0 0.05266002411274788 0.0 17 0.0 0.0 0.0 0.055431604329208295 0.0 18 0.0 0.0 0.0 0.0568173944374385 0.0 19 0.0 0.0 0.0 0.05820318454566871 0.0 20 0.0 0.0 0.0 0.06651792519504995 0.0 21 0.0 0.0 0.0 0.0748326658444312 0.0 22 0.0 0.0 0.0 0.07760424606089161 0.0 23 0.0 0.0 0.0 0.08176161638558224 0.0 24 0.0 0.0 0.0 0.08730477681850307 0.0 25 0.0 0.0 0.0 0.09839109768434473 0.0 26 0.0 0.0 0.0 0.113634788874877 0.0 27 0.0 0.0 0.0 0.23142694807444464 0.0 28 0.0 0.0 0.0 0.34506173694932163 0.0 29 0.0 0.0 0.0 0.46978284669004033 0.0 30 0.0 0.0 0.0 0.6055902772966006 0.0 31 0.0 0.0 0.0 0.7497124485525423 0.0 32 0.0 0.0 0.0 0.9603525450035337 0.0 33 0.0 0.0 0.0 1.2347389864331149 0.0 34 0.0 0.0 0.0 1.6213744266293426 0.0 35 0.0 0.0 0.0 2.13827413699921 0.0 36 0.0 0.0 0.0 2.814539709815551 0.0 37 0.0 0.0 0.0 3.601668491290309 0.0 38 0.0 0.0 0.0 4.379096742007455 0.0 39 0.0 0.0 0.0 5.116337079585926 0.0 40 0.0 0.0 0.0 5.972755366472194 0.0 41 0.0 0.0 0.0 6.861046825847757 0.0 42 0.0 0.0 0.0 7.793683568686687 0.0 43 0.0 0.0013857901082302074 0.0 8.690289768711631 0.0 44 0.0 0.0013857901082302074 0.0 9.56195174678843 0.0 45 0.0 0.0013857901082302074 0.0 10.443314255622843 0.0 46 0.0 0.0013857901082302074 0.0 11.391194689652306 0.0 47 0.0 0.0013857901082302074 0.0 12.29334405011017 0.0 48 0.0 0.0013857901082302074 0.0 13.051371239312093 0.0 49 0.0 0.0013857901082302074 0.0 13.806626848297556 0.0 50 0.0 0.0013857901082302074 0.0 14.560496667174789 0.0 51 0.0 0.0013857901082302074 0.0 15.281107523454498 0.0 52 0.0 0.0013857901082302074 0.0 16.10565263785147 0.0 53 0.0 0.0013857901082302074 0.0 16.89693878965092 0.0 54 0.0 0.0013857901082302074 0.0 17.63417912722939 0.0 55 0.0 0.0013857901082302074 0.0 18.343703662643257 0.0 56 0.0 0.0013857901082302074 0.0 19.067086099139424 0.0 57 0.0 0.0013857901082302074 0.0 19.797397486176745 0.0 58 0.0 0.0013857901082302074 0.0 20.51385097213176 0.0 59 0.0 0.0013857901082302074 0.0 21.140228101051814 0.0 60 0.0 0.0013857901082302074 0.0 21.738889427807266 0.0 61 0.0 0.0013857901082302074 0.0 22.271032829367662 0.0 62 0.0 0.0013857901082302074 0.0 22.84059256385028 0.0 63 0.0 0.0013857901082302074 0.0 23.494685494934938 0.0 64 0.0 0.0013857901082302074 0.0 24.086417871149237 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAATTG 20 0.0069257906 52.5 50 TGCGGAG 20 0.0069257906 52.5 5 TCAGCCG 20 0.0069257906 52.5 3 GTGTCTT 20 0.0069257906 52.5 42 GCAGGTT 40 0.002404487 35.0 47 CATAAGG 50 1.6946759E-4 35.0 29 GAACATA 45 0.0042820075 31.111109 26 CTTCAGG 50 0.0071663656 27.999998 61 TATTATG 50 0.0071663656 27.999998 17 TAGATCT 90 6.0711445E-6 27.222221 39 ATAAGGG 70 0.0012154442 25.0 30 AGATCTC 100 1.3752879E-5 24.499998 40 AGGGTGT 80 0.0026432294 21.875 33 GTAGATC 80 0.0026432294 21.875 38 TCTCGGT 80 0.0026432294 21.875 43 ATCTCGG 85 0.0037569436 20.588234 42 TTACACA 85 0.0037569436 20.588234 4 GATCTCG 90 0.005230022 19.444443 41 CTTATCC 110 7.384673E-4 19.090908 1 TCTTTCT 95 0.007146921 18.421051 1 >>END_MODULE