FastQCFastQC Report
Wed 25 May 2016
SRR1780246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780246_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72161
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT65739.108798381397154No Hit
CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG21833.025179806266543RNA PCR Primer, Index 47 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC18572.5734122309834953No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG16662.308726320311526No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC4360.6042044871883705No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2320.3215033051094081Illumina PCR Primer Index 9 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT1570.21756904699214258No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT1100.15243691190532282No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC930.1288784800654093No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG930.1288784800654093No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG870.12056373941602805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAATG200.006925790652.555
GCTATGG200.006925790652.537
GCAGTAC200.006925790652.515
GCTTATA200.006925790652.53
CCTTTAC200.006925790652.51
ACTACAC200.006925790652.53
GTCAATT200.006925790652.512
CGCAGTA200.006925790652.514
CGGCTAT200.006925790652.535
TCTTTAT407.386134E-752.51
TTCATGT200.006925790652.557
CAATAGT200.006925790652.51
CTTTATA451.6637623E-646.6666642
TACTACA350.00124796440.02
CAGTACT350.00124796440.016
TACTTGC400.00240448735.07
TCTTCTG14450.033.6678250
CCGTCTT14450.033.6678247
CTGCTTG14550.033.43642454
CGTCTTC14550.033.43642448