##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780246_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72161 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.68004877981181 34.0 34.0 34.0 34.0 34.0 2 33.70633721816494 34.0 34.0 34.0 34.0 34.0 3 33.73816881695098 34.0 34.0 34.0 34.0 34.0 4 33.75270575518632 34.0 34.0 34.0 34.0 34.0 5 33.73775307991851 34.0 34.0 34.0 34.0 34.0 6 37.49591884813126 38.0 38.0 38.0 37.0 38.0 7 37.47531214922188 38.0 38.0 38.0 37.0 38.0 8 37.40567619628331 38.0 38.0 38.0 37.0 38.0 9 37.41929851304722 38.0 38.0 38.0 37.0 38.0 10-11 37.37580549050041 38.0 38.0 38.0 37.0 38.0 12-13 37.35341112235141 38.0 38.0 38.0 37.0 38.0 14-15 37.27232854311886 38.0 38.0 38.0 36.0 38.0 16-17 37.298478402461164 38.0 38.0 38.0 36.0 38.0 18-19 37.24216682141323 38.0 38.0 38.0 36.0 38.0 20-21 37.267582211998175 38.0 38.0 38.0 36.0 38.0 22-23 37.27290364601377 38.0 38.0 38.0 36.0 38.0 24-25 37.24966394589876 38.0 38.0 38.0 36.0 38.0 26-27 37.15768212746497 38.0 38.0 38.0 36.0 38.0 28-29 37.148806141821765 38.0 38.0 38.0 36.0 38.0 30-31 37.128081650753174 38.0 38.0 38.0 36.0 38.0 32-33 36.977065173708795 38.0 38.0 38.0 36.0 38.0 34-35 37.03323124679536 38.0 38.0 38.0 36.0 38.0 36-37 37.05180083424564 38.0 38.0 38.0 36.0 38.0 38-39 36.24832665844431 38.0 37.0 38.0 34.0 38.0 40-41 36.63285569767603 38.0 37.0 38.0 34.5 38.0 42-43 36.98136805199485 38.0 37.0 38.0 35.5 38.0 44-45 37.0783802885215 38.0 38.0 38.0 36.0 38.0 46-47 37.023870234614265 38.0 38.0 38.0 35.5 38.0 48-49 37.02915702387716 38.0 38.0 38.0 36.0 38.0 50-51 36.86058951511204 38.0 37.0 38.0 35.0 38.0 52-53 36.85838610883995 38.0 37.5 38.0 35.0 38.0 54-55 36.82652679425175 38.0 37.5 38.0 35.0 38.0 56-57 36.88157730630119 38.0 37.5 38.0 35.0 38.0 58-59 36.74307451393412 38.0 37.0 38.0 34.5 38.0 60-61 36.72332700489184 38.0 37.0 38.0 34.0 38.0 62-63 36.712406978838985 38.0 37.0 38.0 34.0 38.0 64-65 36.539418799628606 38.0 37.0 38.0 34.5 38.0 66-67 36.03755491193304 38.0 37.0 38.0 32.5 38.0 68-69 35.9160626931445 38.0 37.0 38.0 31.5 38.0 70-71 34.98533834065492 38.0 36.5 38.0 27.0 38.0 72-73 33.41697731461593 38.0 34.5 38.0 16.0 38.0 74-75 32.9617452640623 38.0 34.0 38.0 11.0 38.0 76 32.38026080569837 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04650443608151278 1101 2 -0.01962038413945777 1101 3 0.14671528059806604 1101 4 0.07280441320017417 1101 5 0.11131467502983128 1101 6 0.10804060670374582 1101 7 0.1177819087966725 1101 8 0.1131729169200213 1101 9 0.15318361401161695 1101 10-11 0.1378836492770219 1101 12-13 0.06588123187123784 1101 14-15 0.36043759875731496 1101 16-17 0.20791998633514908 1101 18-19 0.2793367841171559 1101 20-21 0.10386367853786993 1101 22-23 0.23951135244385569 1101 24-25 0.2843637592860304 1101 26-27 0.20082601723544968 1101 28-29 -0.13012310253178327 1101 30-31 0.21593546437265587 1101 32-33 -0.11470716074785514 1101 34-35 0.3105759779681634 1101 36-37 0.12943740576459817 1101 38-39 0.13813618501291103 1101 40-41 0.1883062034030374 1101 42-43 0.2601966222507812 1101 44-45 0.03729519931884795 1101 46-47 0.21046712033223258 1101 48-49 0.16621570962312404 1101 50-51 0.15165224262064925 1101 52-53 0.3709178455694726 1101 54-55 0.20895377682781202 1101 56-57 0.028130106706562685 1101 58-59 0.28474428715033184 1101 60-61 0.09564205059869124 1101 62-63 0.24471750372801182 1101 64-65 0.27682042144334673 1101 66-67 0.1790583614648682 1101 68-69 -0.4632204090489367 1101 70-71 -0.5144050121457653 1101 72-73 -1.4978237397722722 1101 74-75 -2.1859424360254494 1101 76 -1.3610960159392889 1102 1 -0.027125233948481764 1102 2 0.08809709608961214 1102 3 -0.017942477787535438 1102 4 0.11144635484184562 1102 5 0.03596697936580284 1102 6 -0.03591903332898028 1102 7 0.046851993334442454 1102 8 -0.15868418697962028 1102 9 0.13414898079645354 1102 10-11 0.23616562364298233 1102 12-13 0.07759380345564182 1102 14-15 0.04685701517399821 1102 16-17 0.08299497951758639 1102 18-19 0.16983310263365325 1102 20-21 0.02268014976775845 1102 22-23 -0.007052596288076529 1102 24-25 -0.1462607293968219 1102 26-27 0.11482928966522365 1102 28-29 0.045265498504491575 1102 30-31 0.044446508823156705 1102 32-33 -0.040286653521235394 1102 34-35 0.08225582525476938 1102 36-37 0.13977282981696248 1102 38-39 0.08606364615280881 1102 40-41 0.5145947226285585 1102 42-43 0.3723591529298105 1102 44-45 0.17445091243583732 1102 46-47 0.2502135070243483 1102 48-49 0.25522579961494074 1102 50-51 0.09930700127895165 1102 52-53 0.20167868549312118 1102 54-55 0.19250981718778348 1102 56-57 0.2616179714461282 1102 58-59 0.39552967029731434 1102 60-61 -0.05171273532985765 1102 62-63 0.15178049800126558 1102 64-65 0.28539691448943927 1102 66-67 0.137375787153438 1102 68-69 -0.1255738314650756 1102 70-71 -0.4757903407522477 1102 72-73 -1.0516961150108841 1102 74-75 -1.201950071694938 1102 76 -1.1302260950230085 1103 1 0.14698290498103717 1103 2 0.13827435270264488 1103 3 0.1486291561961508 1103 4 0.029263743343719284 1103 5 0.15389840708724734 1103 6 0.015696109096090538 1103 7 0.05701635855744058 1103 8 0.11652219921667495 1103 9 0.07375777669103911 1103 10-11 0.10582623300907557 1103 12-13 0.20296257158415898 1103 14-15 0.08866726382908041 1103 16-17 0.13519271360787144 1103 18-19 0.10541333914107298 1103 20-21 -0.0894377568688256 1103 22-23 -0.002354676264275213 1103 24-25 -0.06109078616851349 1103 26-27 0.040778170345497244 1103 28-29 0.0686807252194157 1103 30-31 0.153973741884613 1103 32-33 0.020699537816739166 1103 34-35 0.019906121508832086 1103 36-37 0.04953309954451157 1103 38-39 -0.24535663795359142 1103 40-41 0.1500286914413138 1103 42-43 0.11474207679624016 1103 44-45 0.08585979262028332 1103 46-47 -0.2309204394763853 1103 48-49 -0.3754110846352461 1103 50-51 -0.19356306838412962 1103 52-53 -0.13482378122477456 1103 54-55 0.09125042754551771 1103 56-57 0.0659291497687633 1103 58-59 0.36249548332258286 1103 60-61 0.13415879701017985 1103 62-63 0.3423251592304055 1103 64-65 0.5574424310127313 1103 66-67 0.4233167346706068 1103 68-69 0.4628561459749818 1103 70-71 -0.4153619499448027 1103 72-73 0.017248030562655003 1103 74-75 -0.05149267525990098 1103 76 0.07048293142913309 1104 1 -0.18552997687395134 1104 2 -0.03530864324876859 1104 3 0.138668721893616 1104 4 0.004282145993698805 1104 5 0.009138561669509215 1104 6 -0.17419623540151719 1104 7 -0.15210675517093364 1104 8 -0.19841615190588868 1104 9 -0.09701881513818478 1104 10-11 -0.18033497420476152 1104 12-13 -0.2342958936348296 1104 14-15 -0.3252658425382293 1104 16-17 -0.1558451958510929 1104 18-19 -0.28703467337272315 1104 20-21 -0.34330231336496553 1104 22-23 -0.06828216982335533 1104 24-25 -0.06978603828482477 1104 26-27 -0.07121345644875987 1104 28-29 -0.46137310253178043 1104 30-31 -0.4094693939269405 1104 32-33 -0.16913529839967367 1104 34-35 -0.27064871838811655 1104 36-37 -0.4139836468669742 1104 38-39 -0.7214791996024701 1104 40-41 -0.13456829052408636 1104 42-43 0.1081976343965323 1104 44-45 -0.10055905169329549 1104 46-47 -0.3975794383722189 1104 48-49 -0.36708388551857496 1104 50-51 -0.2888082836951398 1104 52-53 -0.3861924783171631 1104 54-55 -0.4619217292450557 1104 56-57 -0.5373557232529507 1104 58-59 -0.1633832432140423 1104 60-61 -0.3256889210612286 1104 62-63 -0.33278755700073503 1104 64-65 -0.5167777566942959 1104 66-67 -0.49290520128816695 1104 68-69 -0.3502649434618945 1104 70-71 -0.4244252550607399 1104 72-73 0.03398294038967009 1104 74-75 0.42445736154540015 1104 76 0.8953817168542315 1105 1 -0.1029590284049462 1105 2 0.07848896690806129 1105 3 -0.03202769334163946 1105 4 0.06524181739026602 1105 5 0.06977149669565108 1105 6 0.1200998811033358 1105 7 -0.0036284131246304696 1105 8 0.021757485136681964 1105 9 0.30402674124717777 1105 10-11 -0.052254316997895955 1105 12-13 -0.1361116143219121 1105 14-15 -0.0802369030311354 1105 16-17 -0.04979590533577749 1105 18-19 0.07440576725461057 1105 20-21 -0.07687214159498268 1105 22-23 -0.1025428120937022 1105 24-25 0.029075052081118713 1105 26-27 0.05833751442502688 1105 28-29 -0.03449203457061856 1105 30-31 0.032184825639902215 1105 32-33 0.03547168442332804 1105 34-35 -0.11992989837317936 1105 36-37 0.1430551880844817 1105 38-39 -0.4691453683851421 1105 40-41 0.06222035772086798 1105 42-43 -0.27158671959899294 1105 44-45 -0.16571961928104884 1105 46-47 -0.21876129050067306 1105 48-49 -0.03708015138862919 1105 50-51 -0.24663595359805157 1105 52-53 0.12559336933033904 1105 54-55 0.012709525422607726 1105 56-57 -0.1449920189960494 1105 58-59 -0.14550609608932064 1105 60-61 -0.12042846549736907 1105 62-63 -0.05219102974158574 1105 64-65 -0.01689444825517228 1105 66-67 -0.9321322363889948 1105 68-69 -0.35213200843575976 1105 70-71 -1.440388660587253 1105 72-73 -1.5509918542331818 1105 74-75 -1.4350226608593815 1105 76 -1.319144048642407 1106 1 -0.16880656870317523 1106 2 -0.20143856595763765 1106 3 0.03379662031098718 1106 4 -0.09035348153666689 1106 5 0.10605151713509997 1106 6 0.12095926699083037 1106 7 0.1000785595143796 1106 8 0.1213068882118904 1106 9 0.006772130758022854 1106 10-11 0.11778795549711418 1106 12-13 0.2843022845028216 1106 14-15 0.29440889062334463 1106 16-17 0.21892477102414176 1106 18-19 0.1269444396195425 1106 20-21 0.22156702782016424 1106 22-23 0.08855441464481117 1106 24-25 0.21642117555396823 1106 26-27 0.062309270823966756 1106 28-29 0.016049146272052894 1106 30-31 0.07024168446835688 1106 32-33 0.047015327290424125 1106 34-35 -0.19779722777347075 1106 36-37 -0.09879225930716728 1106 38-39 -0.101815968097128 1106 40-41 0.3414162512499246 1106 42-43 0.18412006722685703 1106 44-45 0.3418406538643026 1106 46-47 0.3001800389925151 1106 48-49 0.18678987230255473 1106 50-51 0.3805996110417027 1106 52-53 0.4943628216460283 1106 54-55 0.5386188485981478 1106 56-57 0.2884171880462745 1106 58-59 0.37206486131301375 1106 60-61 0.24899133289534348 1106 62-63 0.27294716879978154 1106 64-65 0.023949608046223148 1106 66-67 -0.0073052748987691984 1106 68-69 0.1542437057835997 1106 70-71 0.09181508354802048 1106 72-73 0.031602097548301344 1106 74-75 -0.16393286664746398 1106 76 -0.3457850111546037 1107 1 0.11063320460475978 1107 2 -0.13024889716960786 1107 3 -0.05629953800183074 1107 4 -0.04640866804663091 1107 5 0.006664700579143812 1107 6 -0.02553118685782607 1107 7 0.1274395480404138 1107 8 0.26933030067066 1107 9 0.08558013930542785 1107 10-11 -0.042545579133808076 1107 12-13 0.002237900241190971 1107 14-15 0.1697630969688646 1107 16-17 0.21962157039236985 1107 18-19 -0.08578840750266892 1107 20-21 0.09545795549238534 1107 22-23 -0.11225154995777586 1107 24-25 0.016939129751023074 1107 26-27 -0.1334100583905098 1107 28-29 0.14754680038083734 1107 30-31 0.2700489033098066 1107 32-33 0.2903260533553649 1107 34-35 0.10579825696662937 1107 36-37 0.10179305216215795 1107 38-39 0.028427447148843044 1107 40-41 -0.05428449664806578 1107 42-43 -0.05556730212325789 1107 44-45 0.05272698266069398 1107 46-47 0.09191832114981224 1107 48-49 0.137677130164775 1107 50-51 0.34316987164466894 1107 52-53 0.1887001654468392 1107 54-55 0.14135030212163002 1107 56-57 -0.06732211608342453 1107 58-59 0.36177545730873106 1107 60-61 -0.2539236111284424 1107 62-63 0.11771188287977452 1107 64-65 -0.16495270068234902 1107 66-67 0.6819454335139241 1107 68-69 0.4634019721467695 1107 70-71 0.01106765009235744 1107 72-73 -0.12623457267978466 1107 74-75 -0.5126925637719992 1107 76 -0.10555181563270111 1108 1 -0.3064992853432642 1108 2 -0.008987741927867887 1108 3 -0.037176266451375284 1108 4 -0.0068872401756863155 1108 5 0.0265725166034656 1108 6 0.12946538076010228 1108 7 0.0192704787062965 1108 8 0.305517414527678 1108 9 0.16094544282607615 1108 10-11 0.021296727426936002 1108 12-13 0.11484454925560783 1108 14-15 -0.07334762187001331 1108 16-17 -0.09407363407952829 1108 18-19 0.24470095211290044 1108 20-21 0.23711726705461444 1108 22-23 0.13228115573997457 1108 24-25 0.1818650883663011 1108 26-27 0.07746078597548234 1108 28-29 0.062301139892461777 1108 30-31 0.011762152304612528 1108 32-33 0.24624446073008244 1108 34-35 0.12197948674009496 1108 36-37 0.14788504000171088 1108 38-39 -0.1992375523608203 1108 40-41 -0.3002637062195106 1108 42-43 -0.011387641438744822 1108 44-45 0.030171329037081307 1108 46-47 0.14627254297975867 1108 48-49 0.0137436203089365 1108 50-51 -0.03659868773554109 1108 52-53 0.06653188065720173 1108 54-55 -0.1822742933476249 1108 56-57 0.162819101921869 1108 58-59 0.18213928980848948 1108 60-61 0.23450435788205226 1108 62-63 -0.13827026873344295 1108 64-65 -0.15627367744022536 1108 66-67 -0.3712074545904187 1108 68-69 -0.258967352164305 1108 70-71 -0.040295495930983805 1108 72-73 -0.5570475568908222 1108 74-75 -0.6728377044996634 1108 76 -0.6296765582040429 1109 1 0.14804616920219615 1109 2 0.07687084393072041 1109 3 0.008278279003171463 1109 4 0.03351680022835524 1109 5 -0.10474061470966944 1109 6 -0.4765952253005068 1109 7 -0.20295174351592493 1109 8 -0.14902804001777525 1109 9 -0.2178424359618063 1109 10-11 -0.28173357560336143 1109 12-13 0.11231929673035523 1109 14-15 0.080692782170388 1109 16-17 0.20138091137501846 1109 18-19 0.05278176019371017 1109 20-21 0.14620817614552806 1109 22-23 -0.03691076345194233 1109 24-25 -0.03278137628016253 1109 26-27 0.07241028092497714 1109 28-29 0.14058396817529228 1109 30-31 0.01428740482985802 1109 32-33 0.24624446073008244 1109 34-35 0.0739996887602885 1109 36-37 0.19333958545626473 1109 38-39 -0.10832846145173392 1109 40-41 0.32347366751786666 1109 42-43 -0.2714886515397552 1109 44-45 0.0352218340875865 1109 46-47 -0.17695978025256665 1109 48-49 0.0970769536422651 1109 50-51 -0.11993202106887679 1109 52-53 -0.044579230453912544 1109 54-55 0.12580651473318483 1109 56-57 0.27645546555823586 1109 58-59 0.09880595647515378 1109 60-61 0.06531243869013537 1109 62-63 -0.06503794550112474 1109 64-65 0.0533222821557402 1109 66-67 -0.05302563640860569 1109 68-69 0.13497204177508593 1109 70-71 0.3738459182104279 1109 72-73 0.2005282006849285 1109 74-75 0.2539299722680113 1109 76 -0.22563615416363803 1110 1 0.09812386927989536 1110 2 -0.09780864324876859 1110 3 -0.01998512426023069 1110 4 0.04755137676293231 1110 5 -0.04374605371510398 1110 6 -0.284773158478437 1110 7 -0.03191444747862704 1110 8 0.12850692501719152 1110 9 0.11451964640027512 1110 10-11 -0.22360420497399502 1110 12-13 0.058973337134396786 1110 14-15 -0.09449661176900293 1110 16-17 -0.1005567343126259 1110 18-19 0.023061480473423046 1110 20-21 0.05573614817349437 1110 22-23 -0.3062629390541147 1110 24-25 0.22829088479209503 1110 26-27 -0.23948268721798627 1110 28-29 0.16362689746821957 1110 30-31 -0.15466170161924708 1110 32-33 0.10490316313878623 1110 34-35 -0.09757179531118965 1110 36-37 -0.2168682622515874 1110 38-39 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2115 68-69 -0.03507589522199339 2115 70-71 0.0037038192547740323 2115 72-73 0.6691551481254407 2115 74-75 0.8266058839269732 2115 76 0.6271287833470609 2116 1 -0.32875574827769327 2116 2 -0.3044850882726706 2116 3 -0.18638016989256556 2116 4 -0.1078973411857902 2116 5 -0.20498029969511578 2116 6 -0.3680521676049082 2116 7 -0.010347737352596198 2116 8 -0.026018281414792455 2116 9 -0.2013212923220138 2116 10-11 0.17820479111465914 2116 12-13 -0.20603372501249595 2116 14-15 -0.3019471767407538 2116 16-17 -0.9080181641257568 2116 18-19 -0.4292418658977155 2116 20-21 -0.0935196101924376 2116 22-23 -0.38265635496023975 2116 24-25 -0.28072693354774714 2116 26-27 -0.39942673328491907 2116 28-29 -0.304416887842514 2116 30-31 -0.2967466646787855 2116 32-33 -0.4795302353835922 2116 34-35 -0.08141034376838263 2116 36-37 -0.4118650027991819 2116 38-39 0.016949744334951333 2116 40-41 -0.18487250608254158 2116 42-43 -0.13983311310455093 2116 44-45 -0.6474951664260189 2116 46-47 -0.34268482733354233 2116 48-49 -0.14419166474499434 2116 50-51 -0.37408370742395647 2116 52-53 -0.556349475275006 2116 54-55 -0.4336867227261507 2116 56-57 -0.6090093521099718 2116 58-59 -0.5513395665517251 2116 60-61 -0.5028265786014288 2116 62-63 -0.33309992119021814 2116 64-65 -0.6601257575634918 2116 66-67 -0.4149499644346264 2116 68-69 -0.501177590137253 2116 70-71 -0.7138103050390114 2116 72-73 -0.4184154733434866 2116 74-75 0.07236859579138155 2116 76 -0.2655265838845793 2117 1 -0.04748052632449884 2117 2 0.02925179631166941 2117 3 0.01801853874343351 2117 4 -0.030516388804834094 2117 5 -0.17057755554660758 2117 6 -0.06407718778246618 2117 7 0.06057639501221246 2117 8 -0.6143960053857427 2117 9 -0.13090159902096588 2117 10-11 -0.07204742841721412 2117 12-13 0.06641381332487128 2117 14-15 0.3028248168024277 2117 16-17 0.031196928691038295 2117 18-19 0.16019518010713085 2117 20-21 -0.16656238662503853 2117 22-23 0.12425446021328668 2117 24-25 0.06002165402286863 2117 26-27 0.26956035307505033 2117 28-29 -0.6801231025317804 2117 30-31 -0.10818734264488938 2117 32-33 -0.0055592910736663725 2117 34-35 0.05055091531151845 2117 36-37 -0.0746979325812589 2117 38-39 0.05004094691767591 2117 40-41 -0.0020133454691446673 2117 42-43 0.1783670483159412 2117 44-45 0.131812743178493 2117 46-47 0.13057990228711702 2117 48-49 0.017531499096811842 2117 50-51 0.03113219249533472 2117 52-53 -0.21918240505709008 2117 54-55 -0.0632266742999974 2117 56-57 -0.07879345219068057 2117 58-59 0.2393182219874248 2117 60-61 -0.083587272709579 2117 62-63 0.19434156387838897 2117 64-65 0.022027188360645766 2117 66-67 0.26236036647739525 2117 68-69 0.20216179646484989 2117 70-71 0.9757578951224062 2117 72-73 1.2387677389244658 2117 74-75 1.1949472882853271 2117 76 0.979630801103319 2118 1 0.014064846541629095 2118 2 -0.16526752084915586 2118 3 -0.15066969126555563 2118 4 -0.18336903929899506 2118 5 -0.025361922123423142 2118 6 -0.1785201347725831 2118 7 -0.13091057713513266 2118 8 0.20860489308685715 2118 9 0.03554044949655122 2118 10-11 -0.04245398726717298 2118 12-13 0.07096233084508441 2118 14-15 0.05782257062093521 2118 16-17 0.10504014522292948 2118 18-19 0.0710779294332724 2118 20-21 0.11690571759778123 2118 22-23 -0.16993916063128012 2118 24-25 0.04626379238764855 2118 26-27 -0.37879993443618076 2118 28-29 0.040002683631740865 2118 30-31 0.14574347496517248 2118 32-33 -0.14774257948337066 2118 34-35 0.184872568501099 2118 36-37 0.027054088982083613 2118 38-39 0.014551279352538415 2118 40-41 0.0883393050250163 2118 42-43 -0.18753591664362546 2118 44-45 0.15657689412189058 2118 46-47 -0.21364741127981546 2118 48-49 -0.3370282493308636 2118 50-51 0.13002033265706103 2118 52-53 -0.34699013192142303 2118 54-55 -0.33321768957404174 2118 56-57 -0.07660342523649888 2118 58-59 -0.37737090649415705 2118 60-61 -0.12632087558468896 2118 62-63 -0.014104078529520336 2118 64-65 0.3348641155843666 2118 66-67 -0.1786212148343722 2118 68-69 0.010281742556486506 2118 70-71 -0.0050372531255717945 2118 72-73 0.31940565446804214 2118 74-75 0.6553021849609806 2118 76 0.5670072611212831 2119 1 -0.10481135339217218 2119 2 0.09880066849212454 2119 3 0.13487317533490994 2119 4 0.028067571094915422 2119 5 -0.00579058812805755 2119 6 0.1514616593353253 2119 7 -0.06379703857174945 2119 8 0.05790975902528572 2119 9 0.06239414032740598 2119 10-11 0.08459417559280524 2119 12-13 -0.13017139970770586 2119 14-15 -0.4579834538742631 2119 16-17 -0.2565536978753826 2119 18-19 0.12275783674873253 2119 20-21 0.1321531522220809 2119 22-23 0.032932405075435156 2119 24-25 -0.13405729334555616 2119 26-27 -0.0037792459224448294 2119 28-29 0.01724531852084965 2119 30-31 -0.024854009311553682 2119 32-33 -0.4015492660110169 2119 34-35 -0.23547665361078884 2119 36-37 -0.11135206791960428 2119 38-39 -0.2546269728818231 2119 40-41 0.040279887613564824 2119 42-43 -0.2180928514334326 2119 44-45 -0.1324416427864108 2119 46-47 0.28612501506906796 2119 48-49 0.05042623593892159 2119 50-51 -0.15887407316882474 2119 52-53 -0.38302952285157943 2119 54-55 -0.21407253896165912 2119 56-57 -0.15147515645133325 2119 58-59 -0.0672293970601956 2119 60-61 0.25616836638816665 2119 62-63 -0.026993022587781468 2119 64-65 -0.16923221013559697 2119 66-67 0.22445309830696658 2119 68-69 -0.31256251431209847 2119 70-71 0.06848972469634163 2119 72-73 0.046255207596146874 2119 74-75 -0.06554769918334813 2119 76 -1.192674963307713 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 1.0 22 4.0 23 10.0 24 15.0 25 33.0 26 78.0 27 152.0 28 245.0 29 407.0 30 697.0 31 1109.0 32 1788.0 33 2841.0 34 4869.0 35 7682.0 36 10583.0 37 41646.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.21045994373692 16.25116059921564 12.828259031887029 37.710120425160405 2 17.684067571125677 19.57151369853522 38.038552680810966 24.70586604952814 3 16.614237607571958 17.950139271905876 35.5718462881612 29.863776832360973 4 13.518382505785672 27.050622912653647 32.60625545654855 26.824739125012126 5 13.341001371932206 29.522872465736338 42.09060295727609 15.04552320505536 6 25.1950499577334 40.03270464655424 17.210127354110945 17.56211804160142 7 24.25825584456978 25.30868474660828 23.559817630021758 26.873241778800182 8 21.2219897174374 38.89219938748077 15.928271503998005 23.957539391083827 9 20.493064120508308 16.338465376034144 16.23037374759219 46.93809675586535 10-11 24.334474300522444 28.48768725488837 24.154321586452514 23.023516858136666 12-13 23.604162913485123 18.759440695112318 27.0804173999806 30.555978991421963 14-15 20.228378209836336 17.86491318024972 20.7037042169593 41.20300439295465 16-17 27.08596056041352 26.339019692077436 18.35340419340087 28.221615554108176 18-19 30.19220908801153 24.4058424910963 19.372652818004184 26.029295602887988 20-21 23.667216363409597 21.77491997062125 18.810714929116838 35.74714873685232 22-23 21.249012624547888 25.94684109144829 20.57759731711035 32.226548966893475 24-25 28.642202851956043 26.41246656781364 20.592148113246765 24.35318246698355 26-27 28.034533889497098 27.879325397375315 21.61070384279597 22.47543687033162 28-29 22.194814373415003 26.006430066102187 25.17010573578526 26.628649824697554 30-31 26.988262357783288 20.477820429317774 29.64343620515237 22.890481007746565 32-33 22.99164368564737 20.149388173667216 28.176577375590696 28.682390765094716 34-35 20.373193276146395 30.703565637948476 25.03706988539516 23.88617120050997 36-37 27.884868557808236 29.363506603289867 21.86846080292679 20.88316403597511 38-39 21.190809440002216 24.710716314906943 28.609636784412633 25.488837460678205 40-41 20.5845262676515 18.73241778800183 29.761228364351933 30.921827579994734 42-43 26.666759052673882 18.69846593035019 28.857000318731725 25.777774698244205 44-45 19.4329346877122 25.463893238730062 32.423331162262166 22.679840911295575 46-47 24.003270464655422 20.860991394243428 29.454968750433057 25.68076939066809 48-49 23.788472997879744 19.474508390959105 24.54303571181109 32.19398289935007 50-51 21.337010296420505 19.67336927149014 29.20552653095162 29.78409390113773 52-53 16.418841202311498 19.495988137636676 39.28091351283934 24.804257147212482 54-55 17.448483252726543 21.35017530244869 32.98319036598717 28.2181510788376 56-57 22.381203142971966 23.21544878812655 25.666911489585786 28.7364365793157 58-59 18.18641648535913 23.574368426158173 32.793337121159624 25.44587796732307 60-61 22.31537811283103 28.47244356369784 26.4013802469478 22.81079807652333 62-63 17.549645930627346 38.592175829048934 20.98501960893003 22.87315863139369 64-65 16.197114784994664 40.77202366929505 20.019123903493576 23.01173764221671 66-67 16.40775488144566 39.98628067792852 20.091184989121547 23.514779451504275 68-69 16.29550588267901 40.20454261997478 20.113357630853233 23.38659386649298 70-71 16.57404969443328 38.20761907401505 20.955918016657197 24.262413214894472 72-73 16.862294036945162 34.25950305566719 22.478901345602196 26.399301561785453 74-75 17.37642216709857 32.73790551683042 23.11359321517163 26.772079100899376 76 17.064619392746774 33.46960269397597 23.23415695458766 26.231620958689593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 1.0 15 1.5 16 2.0 17 2.0 18 3.0 19 8.0 20 10.0 21 8.0 22 23.0 23 39.0 24 44.5 25 49.0 26 80.0 27 158.0 28 234.5 29 264.0 30 304.5 31 402.0 32 583.0 33 707.0 34 774.0 35 993.0 36 1244.5 37 1344.0 38 1591.5 39 3454.5 40 6090.0 41 6788.0 42 6466.0 43 5761.5 44 4555.0 45 3828.5 46 3604.0 47 3532.5 48 3255.0 49 2865.0 50 2681.0 51 2478.0 52 2063.5 53 1719.0 54 1586.0 55 1593.0 56 1517.5 57 1473.5 58 1512.0 59 1460.0 60 1473.0 61 1626.5 62 1715.0 63 1701.0 64 1685.5 65 1676.0 66 1573.5 67 1479.0 68 1297.5 69 954.5 70 629.0 71 465.0 72 364.0 73 173.5 74 52.0 75 20.0 76 15.0 77 6.0 78 1.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 72161.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.01600587575006 #Duplication Level Percentage of deduplicated Percentage of total 1 63.85297377170299 28.74405842491096 2 19.868242827238024 17.88777871703552 3 7.646841521980052 10.326907886531506 4 3.189262406107622 5.74271420850598 5 1.887082871567541 4.247446681725585 6 1.0220416204900873 2.760493895594573 7 0.7203546361285557 2.26992419728108 8 0.5171776874769117 1.8625019054613987 9 0.2862947912818618 1.1599063205886837 >10 0.9573944095554735 5.828633195216253 >50 0.02770594754340599 0.8578040769944985 >100 0.012313754463735992 1.295713751195244 >500 0.0 0.0 >1k 0.009235315847801994 7.907318357561564 >5k 0.003078438615933998 9.108798381397154 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT 6573 9.108798381397154 No Hit CACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTTCTG 2183 3.025179806266543 RNA PCR Primer, Index 47 (96% over 25bp) CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 1857 2.5734122309834953 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 1666 2.308726320311526 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 436 0.6042044871883705 No Hit ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT 232 0.3215033051094081 Illumina PCR Primer Index 9 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT 157 0.21756904699214258 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT 110 0.15243691190532282 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC 93 0.1288784800654093 No Hit CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 93 0.1288784800654093 No Hit CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG 87 0.12056373941602805 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0055431604329208295 0.0 2 0.0 0.0 0.0 0.0055431604329208295 0.0 3 0.0 0.0 0.0 0.0055431604329208295 0.0 4 0.0 0.0 0.0 0.018015271406992696 0.0 5 0.0 0.0 0.0 0.02078685162345311 0.0 6 0.0 0.0 0.0 0.023558431839913525 0.0 7 0.0 0.0 0.0 0.023558431839913525 0.0 8 0.0 0.0 0.0 0.024944221948143733 0.0 9 0.0 0.0 0.0 0.029101592272834355 0.0 10 0.0 0.0 0.0 0.038802123030445806 0.0 11 0.0 0.0 0.0 0.040187913138676014 0.0 12 0.0 0.0 0.0 0.040187913138676014 0.0 13 0.0 0.0 0.0 0.04157370324690622 0.0 14 0.0 0.0 0.0 0.044345283463366636 0.0 15 0.0 0.0 0.0 0.049888443896287465 0.0 16 0.0 0.0 0.0 0.055431604329208295 0.0 17 0.0 0.0 0.0 0.05820318454566871 0.0 18 0.0 0.0 0.0 0.06097476476212913 0.0 19 0.0 0.0 0.0 0.06374634497858954 0.0 20 0.0 0.0 0.0 0.07206108562797078 0.0 21 0.0 0.0 0.0 0.07899003616912183 0.0 22 0.0 0.0 0.0 0.08453319660204266 0.0 23 0.0 0.0 0.0 0.08730477681850307 0.0 24 0.0 0.0 0.0 0.0928479372514239 0.0 25 0.0 0.0 0.0 0.10393425811726556 0.0 26 0.0 0.0 0.0 0.11917794930779783 0.0 27 0.0 0.0 0.0 0.23558431839913527 0.0 28 0.0 0.0 0.0 0.3478333171657821 0.0 29 0.0 0.0 0.0 0.4794833774476518 0.0 30 0.0 0.0 0.0 0.6166765981624424 0.0 31 0.0 0.0 0.0 0.7691135100677652 0.0 32 0.0 0.0 0.0 0.9866825570599077 0.0 33 0.0 0.0 0.0 1.2666121589224095 0.0 34 0.0 0.0 0.0 1.6684912903091698 0.0 35 0.0 0.0 0.0 2.197863111653109 0.0 36 0.0 0.0 0.0 2.886600795443522 0.0 37 0.0 0.0 0.0 3.709760119732265 0.0 38 0.0 0.0 0.0 4.509361012181095 0.0 39 0.0 0.0 0.0 5.259073460733637 0.0 40 0.0 0.0 0.0 6.158451240975042 0.0 41 0.0 0.0 0.0 7.063372181649368 0.0 42 0.0 0.0 0.0 8.005709455245908 0.0 43 0.0 0.0 0.0 8.94250356840953 0.0 44 0.0 0.0 0.0 9.832180817893322 0.0013857901082302074 45 0.0 0.0 0.0 10.732944388242958 0.0013857901082302074 46 0.0 0.0 0.0 11.711312204653483 0.0013857901082302074 47 0.0 0.0 0.0 12.643948947492413 0.0013857901082302074 48 0.0 0.0 0.0 13.41721982788487 0.0013857901082302074 49 0.0 0.0 0.0 14.183561757736173 0.0013857901082302074 50 0.0 0.0 0.0 14.942974737046328 0.0013857901082302074 51 0.0 0.0 0.0 15.671900333975415 0.0013857901082302074 52 0.0 0.0 0.0 16.518618090104074 0.0013857901082302074 53 0.0 0.0 0.0 17.323762142985824 0.0013857901082302074 54 0.0 0.0 0.0 18.069317221213677 0.0013857901082302074 55 0.0 0.0 0.0 18.77329859619462 0.0013857901082302074 56 0.0 0.0 0.0 19.50222419312371 0.0013857901082302074 57 0.0 0.0 0.0 20.23807874059395 0.0013857901082302074 58 0.0 0.0 0.0 20.953146436440736 0.0013857901082302074 59 0.0 0.0 0.0 21.585066725793713 0.0013857901082302074 60 0.0 0.0 0.0 22.18511384265739 0.0013857901082302074 61 0.0 0.0 0.0 22.73250093540832 0.0013857901082302074 62 0.0 0.0 0.0 23.30898962043209 0.0013857901082302074 63 0.0 0.0 0.0 23.96585413173321 0.0013857901082302074 64 0.0 0.0 0.0 24.556200717839275 0.0013857901082302074 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAATG 20 0.0069257906 52.5 55 GCTATGG 20 0.0069257906 52.5 37 GCAGTAC 20 0.0069257906 52.5 15 GCTTATA 20 0.0069257906 52.5 3 CCTTTAC 20 0.0069257906 52.5 1 ACTACAC 20 0.0069257906 52.5 3 GTCAATT 20 0.0069257906 52.5 12 CGCAGTA 20 0.0069257906 52.5 14 CGGCTAT 20 0.0069257906 52.5 35 TCTTTAT 40 7.386134E-7 52.5 1 TTCATGT 20 0.0069257906 52.5 57 CAATAGT 20 0.0069257906 52.5 1 CTTTATA 45 1.6637623E-6 46.666664 2 TACTACA 35 0.001247964 40.0 2 CAGTACT 35 0.001247964 40.0 16 TACTTGC 40 0.002404487 35.0 7 TCTTCTG 1445 0.0 33.66782 50 CCGTCTT 1445 0.0 33.66782 47 CTGCTTG 1455 0.0 33.436424 54 CGTCTTC 1455 0.0 33.436424 48 >>END_MODULE