FastQCFastQC Report
Wed 25 May 2016
SRR1780245_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780245_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414216
Sequences flagged as poor quality0
Sequence length50
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGG250.00236332935.162536
ACCTTTG300.00576726229.30564942
ACCCGAG450.001402393824.42726928
AGTGATT602.8820278E-421.98454330
TTTACAC602.889098E-421.9765822
CAACCGT500.002593590221.976586
TAGAATA550.004453142620.01979414
CTAATTC550.00449697319.98594930
CTATACA1001.1312823E-619.7789231
GTATTAG1152.036104E-719.110071
CTAGAAT708.0703315E-418.8712413
GTGATTA708.162671E-418.83934431
GCAACTC600.00736707718.34925737
TTCCCTA600.007434155318.32045226
TTCGATT600.007444519518.3160342
CTATAAC600.007449705618.3138184
GCTAAAG600.007449705618.3138181
CTGACAC750.00129816317.5812663
GTAATAT902.2290088E-417.0928963
TATAAGA1053.799224E-516.7440622
Produced by FastQC (version 0.11.5)