FastQCFastQC Report
Wed 25 May 2016
SRR1780245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780245_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414216
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT177234.27868551673523No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC52761.273731579658922No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG46191.115118682040288No Hit
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG38250.9234312532591691RNA PCR Primer, Index 14 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC9530.23007319852444136No Hit
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT4790.11564014910095215RNA PCR Primer, Index 35 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCAC207.8390853E-444.013164
TCTTCTG5150.037.1627144
GTCTTCT5300.036.11092843
CTTTATA1300.035.549092
AGATTCT250.00235174135.1977820
TGCTCTA250.00235174135.1977810
CGTCTTC5500.034.79780242
CCGTCTT5550.034.4843141
ATGCCGT26050.032.85013244
GCCGTCT6050.032.36159540
AACTGAC300.005742481429.33148433
TATACCG300.005742481429.3314846
TCTATTA300.005742481429.33148440
TATGCCG30400.028.1495443
TTTATAC1800.026.8969333
TGCCGTC7550.025.64076639
TCTTTAT1900.025.5151981
GCCAACA450.001397177724.44290225
CTAATAT450.001397177724.44290221
ACATCCA651.8105447E-523.69081336