Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780245_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 414216 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 60 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGT | 17723 | 4.27868551673523 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC | 5276 | 1.273731579658922 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCG | 4619 | 1.115118682040288 | No Hit |
CACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTTCTG | 3825 | 0.9234312532591691 | RNA PCR Primer, Index 14 (96% over 25bp) |
TCTTTATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCC | 953 | 0.23007319852444136 | No Hit |
ATACACATCTCCGAGCCCACGAGACTGGGTTTCATCTCGTATGCCGTCTT | 479 | 0.11564014910095215 | RNA PCR Primer, Index 35 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTAGCAC | 20 | 7.8390853E-4 | 44.01316 | 4 |
TCTTCTG | 515 | 0.0 | 37.16271 | 44 |
GTCTTCT | 530 | 0.0 | 36.110928 | 43 |
CTTTATA | 130 | 0.0 | 35.54909 | 2 |
AGATTCT | 25 | 0.002351741 | 35.19778 | 20 |
TGCTCTA | 25 | 0.002351741 | 35.19778 | 10 |
CGTCTTC | 550 | 0.0 | 34.797802 | 42 |
CCGTCTT | 555 | 0.0 | 34.48431 | 41 |
ATGCCGT | 2605 | 0.0 | 32.850132 | 44 |
GCCGTCT | 605 | 0.0 | 32.361595 | 40 |
AACTGAC | 30 | 0.0057424814 | 29.331484 | 33 |
TATACCG | 30 | 0.0057424814 | 29.331484 | 6 |
TCTATTA | 30 | 0.0057424814 | 29.331484 | 40 |
TATGCCG | 3040 | 0.0 | 28.14954 | 43 |
TTTATAC | 180 | 0.0 | 26.896933 | 3 |
TGCCGTC | 755 | 0.0 | 25.640766 | 39 |
TCTTTAT | 190 | 0.0 | 25.515198 | 1 |
GCCAACA | 45 | 0.0013971777 | 24.442902 | 25 |
CTAATAT | 45 | 0.0013971777 | 24.442902 | 21 |
ACATCCA | 65 | 1.8105447E-5 | 23.690813 | 36 |