FastQCFastQC Report
Wed 25 May 2016
SRR1780244_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780244_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108926
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTTT200.006935234652.533
CGCATCT200.006935234652.524
TAGAACC200.006935234652.565
TCGAGAT200.006935234652.59
GCATACT200.006935234652.566
TTTGATA350.001250770540.037
CGGACTA350.001250770540.024
AAGAGGT459.1471404E-538.88888516
ACATGTC400.002409861435.020
ATTACTT450.004291520531.1111098
TTATGCT450.004291520531.11110919
CAGATAA450.004291520531.11110914
GCAGATA450.004291520531.11110913
CTAATAG450.004291520531.11110957
TAATAGC604.955074E-429.16666658
ACACTCT604.955074E-429.1666666
AATCCTC500.007182189727.99999823
CGGCGGA500.007182189727.99999821
GGCAGAT500.007182189727.99999812
AAAGTTA500.007182189727.99999852