##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780244_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 108926 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.814718249086535 34.0 32.0 34.0 21.0 34.0 2 30.76877880395865 34.0 32.0 34.0 21.0 34.0 3 30.847658043075114 34.0 33.0 34.0 21.0 34.0 4 30.666103593265152 34.0 32.0 34.0 21.0 34.0 5 30.50968547454235 34.0 32.0 34.0 21.0 34.0 6 33.85891339074234 37.0 34.0 38.0 21.0 38.0 7 34.19693186199805 38.0 35.0 38.0 22.0 38.0 8 34.17691827479206 38.0 35.0 38.0 22.0 38.0 9 34.48033527348842 38.0 36.0 38.0 24.0 38.0 10-11 34.23309402713769 38.0 35.5 38.0 22.0 38.0 12-13 34.284821805629505 38.0 35.5 38.0 23.0 38.0 14-15 34.26079173016544 38.0 36.0 38.0 22.5 38.0 16-17 34.35446082661623 38.0 36.0 38.0 23.0 38.0 18-19 34.33055468850412 38.0 36.0 38.0 23.0 38.0 20-21 34.36792868552962 38.0 36.0 38.0 23.0 38.0 22-23 34.359087821089545 38.0 36.0 38.0 23.0 38.0 24-25 34.39606705469768 38.0 36.0 38.0 23.0 38.0 26-27 34.40931917081322 38.0 36.0 38.0 23.0 38.0 28-29 34.52359858986835 38.0 36.0 38.0 23.0 38.0 30-31 34.519710629234524 38.0 36.0 38.0 23.5 38.0 32-33 34.62842663826818 38.0 36.0 38.0 24.0 38.0 34-35 34.55203532673558 38.0 36.0 38.0 24.0 38.0 36-37 34.521510015974144 38.0 36.0 38.0 23.5 38.0 38-39 34.50608670106311 38.0 36.0 38.0 23.0 38.0 40-41 34.55673117529332 38.0 36.0 38.0 24.0 38.0 42-43 34.61247544204322 38.0 36.0 38.0 24.0 38.0 44-45 34.651731450709654 38.0 36.0 38.0 24.5 38.0 46-47 34.59674457888842 38.0 36.0 38.0 24.0 38.0 48-49 34.568408828011684 38.0 36.0 38.0 24.0 38.0 50-51 34.51040155702036 38.0 36.0 38.0 23.5 38.0 52-53 34.550047738831864 38.0 36.0 38.0 24.0 38.0 54-55 34.53753465655582 38.0 36.0 38.0 24.0 38.0 56-57 34.4737115105668 38.0 36.0 38.0 24.0 38.0 58-59 34.55429833097699 38.0 36.0 38.0 24.0 38.0 60-61 34.56216605769054 38.0 36.0 38.0 24.0 38.0 62-63 34.4210610873437 38.0 36.0 38.0 23.0 38.0 64-65 34.38656519104713 38.0 36.0 38.0 23.5 38.0 66-67 34.27594421901107 38.0 35.5 38.0 23.0 38.0 68-69 34.23082184235169 38.0 36.0 38.0 23.0 38.0 70-71 34.17909865413216 38.0 35.5 38.0 22.5 38.0 72-73 34.17872684207627 38.0 36.0 38.0 23.0 38.0 74-75 34.161494959881026 38.0 36.0 38.0 22.0 38.0 76 32.454648109725866 37.0 31.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0910845740394528 1101 2 -0.013946104504697132 1101 3 -0.5554661728225092 1101 4 -0.6505723892463848 1101 5 -0.39811567014535143 1101 6 -0.48771615967270776 1101 7 -1.3812027824624238 1101 8 -1.1819638101376526 1101 9 -1.157464360287122 1101 10-11 -1.2435910641690668 1101 12-13 -0.5577517207209368 1101 14-15 -0.47311836077401637 1101 16-17 -0.23299089840364218 1101 18-19 -0.49791930401889317 1101 20-21 -0.6670994834406159 1101 22-23 -0.528275384105676 1101 24-25 -0.4910118306552036 1101 26-27 -1.0020921677397894 1101 28-29 -1.3635640554998503 1101 30-31 -0.825870821964152 1101 32-33 -0.7288380143442055 1101 34-35 -0.8342418768433504 1101 36-37 -0.7391136076222651 1101 38-39 -0.40712346684844647 1101 40-41 -0.44421899819572275 1101 42-43 -0.6146925271411448 1101 44-45 -0.6730326225942207 1101 46-47 -0.459385485546683 1101 48-49 -0.45821611432800324 1101 50-51 -0.5888617703991486 1101 52-53 -0.4086402186377214 1101 54-55 -0.35218807541517094 1101 56-57 -0.3803934055407794 1101 58-59 -0.18825412037243439 1101 60-61 -0.5202462537675316 1101 62-63 -0.37311847521886676 1101 64-65 -0.28518993817704796 1101 66-67 -0.4628976030055867 1101 68-69 -0.9869756392706392 1101 70-71 -0.7504757505632966 1101 72-73 -0.28477686730175833 1101 74-75 -0.5811337426604766 1101 76 -2.3156248804252364 1102 1 -0.2898833728382506 1102 2 -0.5670992576578513 1102 3 0.015234963096020238 1102 4 -0.36416424196867325 1102 5 -0.9922924556264867 1102 6 -0.674946868642543 1102 7 -0.8522825578900282 1102 8 -0.5824077323207035 1102 9 -0.4028227621672613 1102 10-11 -0.7844580180795049 1102 12-13 -0.4219245892416268 1102 14-15 -0.25062195132109366 1102 16-17 -0.3644702037959888 1102 18-19 -0.4603034272725779 1102 20-21 -0.1977301140712484 1102 22-23 -0.40500863909980467 1102 24-25 -0.8943673600976965 1102 26-27 -0.8044554005378117 1102 28-29 -0.7179314663889969 1102 30-31 -0.6177235442516533 1102 32-33 -0.5490103606035106 1102 34-35 -0.601718048667351 1102 36-37 -0.3642213240343253 1102 38-39 -0.4685544630620555 1102 40-41 -0.5969543422354135 1102 42-43 -0.8628841970719492 1102 44-45 -0.598819278815661 1102 46-47 -0.9637333116336393 1102 48-49 -0.8927506488625383 1102 50-51 -0.6714182399121427 1102 52-53 -0.09803831238364324 1102 54-55 -0.6879238111509025 1102 56-57 -0.5367584662536657 1102 58-59 -0.390565127031266 1102 60-61 -0.2294902804028638 1102 62-63 -0.3887863169736647 1102 64-65 -0.24659482132106092 1102 66-67 -0.3055663587178259 1102 68-69 -0.08846408423734431 1102 70-71 -0.30891680030869395 1102 72-73 0.056024803934690226 1102 74-75 -0.5815515517739342 1102 76 -0.6903213159912376 1103 1 -0.1501172909669073 1103 2 0.13114635637723637 1103 3 -0.47675588358590204 1103 4 0.6275214941106562 1103 5 0.051465489962122746 1103 6 -0.44369845776026295 1103 7 -0.2668515891638634 1103 8 -0.7956037658828734 1103 9 -0.3869570108324041 1103 10-11 -0.2986329481583212 1103 12-13 -0.14029479010611112 1103 14-15 -0.6984226123940545 1103 16-17 -0.6372899342816183 1103 18-19 -0.5867844849893373 1103 20-21 -0.43077104974376823 1103 22-23 -0.4134500324382202 1103 24-25 -0.30751254189276267 1103 26-27 -0.3105321867061335 1103 28-29 -0.5918317969254829 1103 30-31 -0.7129816169696781 1103 32-33 -0.5595239634512126 1103 34-35 -0.6060531618125324 1103 36-37 -0.4675394018375272 1103 38-39 -0.5131768886913406 1103 40-41 -0.355827975594174 1103 42-43 -0.33134338852885037 1103 44-45 -0.5306270591360303 1103 46-47 -0.6362860703420097 1103 48-49 -0.752984566991195 1103 50-51 -0.8812199180204559 1103 52-53 -0.46348453663993183 1103 54-55 -0.33499983454271387 1103 56-57 -0.2764736963579111 1103 58-59 -0.8606897138606584 1103 60-61 -0.3737313177789119 1103 62-63 -0.41713617713130446 1103 64-65 -0.2486797437721151 1103 66-67 -0.5507354408434253 1103 68-69 -0.7344467946365327 1103 70-71 -0.21866762664991768 1103 72-73 -0.3142141993706744 1103 74-75 -0.5156603745555302 1103 76 -0.6013052976846005 1104 1 -1.1646709082490183 1104 2 -0.85359595265313 1104 3 -0.9601314613404881 1104 4 -0.9083117149743778 1104 5 -1.3527473556552252 1104 6 -1.014602819703839 1104 7 -0.9209691777529017 1104 8 -0.8701278949023035 1104 9 -0.9527837590059534 1104 10-11 -1.002321465958957 1104 12-13 -1.3752193299732483 1104 14-15 -1.2249537655839333 1104 16-17 -0.9126904279615218 1104 18-19 -1.4512834330204214 1104 20-21 -1.346833041361144 1104 22-23 -1.1523215265654159 1104 24-25 -1.5757977497187525 1104 26-27 -1.632003949209647 1104 28-29 -1.0366365614335393 1104 30-31 -1.4811550013276502 1104 32-33 -1.5166541591008667 1104 34-35 -1.1372601926095456 1104 36-37 -2.0222210776985676 1104 38-39 -1.1870748062898713 1104 40-41 -1.1788445586003249 1104 42-43 -0.7229367486998228 1104 44-45 -0.7414764204997795 1104 46-47 -0.9235025771425356 1104 48-49 -1.0461028679371367 1104 50-51 -1.0887766994925485 1104 52-53 -1.3403793231813594 1104 54-55 -1.049680185088576 1104 56-57 -0.9915640662865073 1104 58-59 -1.2614236071396547 1104 60-61 -1.1108467692972255 1104 62-63 -1.40666618723683 1104 64-65 -1.2397918488696078 1104 66-67 -1.1905686167955878 1104 68-69 -1.0559723980691018 1104 70-71 -1.0061988958048573 1104 72-73 -1.199901623786289 1104 74-75 -1.191823752783911 1104 76 -0.762604437886381 1105 1 -0.7318373958267586 1105 2 -0.5693981082325621 1105 3 -0.00987748068209271 1105 4 0.014696327746470672 1105 5 -0.25943388491184294 1105 6 -0.6292197322107569 1105 7 -0.4075049466956244 1105 8 -0.31828979129122104 1105 9 0.015493079911699681 1105 10-11 -0.4486884028871003 1105 12-13 -1.3955377826449293 1105 14-15 -0.5907144050942037 1105 16-17 -0.6839979399279201 1105 18-19 -1.0342789395164544 1105 20-21 -0.7977301140712498 1105 22-23 -0.9470001433476796 1105 24-25 -1.0170810032760969 1105 26-27 -1.0930236164298677 1105 28-29 -0.9754776932755505 1105 30-31 -0.42640670267244474 1105 32-33 -0.4539079118278977 1105 34-35 -0.857515150116626 1105 36-37 -0.5299259717104903 1105 38-39 -0.3097588608631483 1105 40-41 -0.6112222083023795 1105 42-43 -0.8364099342033882 1105 44-45 -0.8181346211444946 1105 46-47 -0.8602475545122061 1105 48-49 -0.9051069707016168 1105 50-51 -0.9206936022309833 1105 52-53 -0.5779733448674023 1105 54-55 -0.6956824318405523 1105 56-57 -0.6603716596569669 1105 58-59 -0.9601553319288172 1105 60-61 -0.5791154678091601 1105 62-63 -0.7804654773934558 1105 64-65 -1.1238561906364026 1105 66-67 -1.2873254791576016 1105 68-69 -1.2495960182703314 1105 70-71 -0.8818053560308385 1105 72-73 -0.8760966353456681 1105 74-75 -0.6218813868563942 1105 76 -0.5306761385799419 1106 1 -0.29568047428752564 1106 2 -0.3265195475129232 1106 3 0.31958278918297367 1106 4 -0.18445409704113658 1106 5 -0.40098810780039784 1106 6 -0.12422223096138651 1106 7 -1.2580796593393018 1106 8 -0.646175848262736 1106 9 -0.1535473998484207 1106 10-11 -0.6090956992389209 1106 12-13 -0.4219245892416268 1106 14-15 -0.19120166146601036 1106 16-17 -0.31809339220178146 1106 18-19 -0.3211729924899771 1106 20-21 -0.6064257662451595 1106 22-23 -0.3267477695345846 1106 24-25 -0.30741083835856386 1106 26-27 -0.4508322121320134 1106 28-29 -0.22372856783827189 1106 30-31 -0.4626510804835391 1106 32-33 -0.004082824371621996 1106 34-35 -0.6162108022905386 1106 36-37 -0.49320683128070186 1106 38-39 -0.6656559123374137 1106 40-41 -0.552026806003532 1106 42-43 -0.6599856463473088 1106 44-45 -0.562587394757692 1106 46-47 -0.6028637464162543 1106 48-49 -0.310141953210362 1106 50-51 -0.536635631216491 1106 52-53 -0.5241252689053866 1106 54-55 -0.6690832314407515 1106 56-57 -0.5527004952391721 1106 58-59 -0.07897092413271878 1106 60-61 -0.05267868619996818 1106 62-63 -0.6177718242200427 1106 64-65 -0.46833395175584513 1106 66-67 -0.7780301268337695 1106 68-69 -0.3841162581503923 1106 70-71 -0.2480472350913061 1106 72-73 -0.23238099316675687 1106 74-75 -0.6974935807594491 1106 76 -0.17148073628109017 1107 1 -0.20702622998111053 1107 2 -0.23662306718166093 1107 3 -0.47495137230770723 1107 4 -1.1096714883454837 1107 5 -0.8387934908231713 1107 6 -1.3229799949365386 1107 7 -0.8095082307678751 1107 8 -0.5237741919480357 1107 9 -0.4191374619602186 1107 10-11 -0.3237540843320872 1107 12-13 -0.42391216688137945 1107 14-15 -0.7380132556689105 1107 16-17 -0.4796875951003301 1107 18-19 -0.5262247523243389 1107 20-21 -0.5901110664521951 1107 22-23 -0.4184040842343819 1107 24-25 -0.6510547306981067 1107 26-27 -0.6507493963970177 1107 28-29 -0.4104366423724315 1107 30-31 -0.8630651591584879 1107 32-33 -0.4952215407277336 1107 34-35 -0.4075151501166232 1107 36-37 -0.4616747401833905 1107 38-39 -0.67112175084673 1107 40-41 -0.3082172821940006 1107 42-43 -0.6437744662230855 1107 44-45 -0.4132085127701117 1107 46-47 -0.5889092950705006 1107 48-49 -0.5579887441006335 1107 50-51 -0.4445031260405088 1107 52-53 -0.48669255669006617 1107 54-55 -0.8093523825794762 1107 56-57 -0.6568412819886547 1107 58-59 -1.3368384189567308 1107 60-61 -0.70505963858092 1107 62-63 -0.5816641637645574 1107 64-65 -0.4831993761864837 1107 66-67 -0.622005282113264 1107 68-69 -0.6097684320634329 1107 70-71 -0.5168464069339507 1107 72-73 -0.5567288192537134 1107 74-75 -0.7955267070534475 1107 76 -0.1590997839001389 1108 1 -0.3988654087663974 1108 2 -0.8008317925879886 1108 3 -0.7151737846259287 1108 4 0.055717733211402276 1108 5 -0.3509577337658598 1108 6 -0.18996032000069363 1108 7 0.05329758616967695 1108 8 -0.1014131996469203 1108 9 -0.28736671773438616 1108 10-11 0.3504460867543102 1108 12-13 -0.06764185037154391 1108 14-15 0.35803464852270395 1108 16-17 0.08827647675595074 1108 18-19 0.2853834598227962 1108 20-21 0.16584274022017098 1108 22-23 -0.08789937935841863 1108 24-25 -0.20350126619853626 1108 26-27 -4.2433223962490274E-4 1108 28-29 0.2285625021955724 1108 30-31 0.12692628652158078 1108 32-33 -0.44277500522730406 1108 34-35 0.1170357480869626 1108 36-37 -0.30452332872060595 1108 38-39 0.09993898482478158 1108 40-41 0.20356201635176063 1108 42-43 0.21761133360583074 1108 44-45 -0.08929918856138386 1108 46-47 -0.022758739039701936 1108 48-49 0.03669046890193073 1108 50-51 -0.05143212518507312 1108 52-53 -0.5694042761083651 1108 54-55 0.2653696020227514 1108 56-57 -0.16830407069695497 1108 58-59 0.0738972525573871 1108 60-61 -0.12084235885465944 1108 62-63 -0.2731697240725097 1108 64-65 0.06743364348844949 1108 66-67 0.14370900360101757 1108 68-69 0.1064591128467427 1108 70-71 -0.07536650958579116 1108 72-73 -0.5301419929064082 1108 74-75 0.044079794229709535 1108 76 0.09099431361910604 1109 1 -0.06598400176907049 1109 2 0.05302652850567213 1109 3 0.10533613892319948 1109 4 -0.23080356381718303 1109 5 -0.2542831583883114 1109 6 0.5099041020985027 1109 7 0.10670021487453596 1109 8 0.29112248370335436 1109 9 0.34194070515567887 1109 10-11 0.3487796083902879 1109 12-13 0.26944812062984624 1109 14-15 0.08953391414240741 1109 16-17 0.007419323138663003 1109 18-19 0.39407174363253006 1109 20-21 0.1865466154885027 1109 22-23 0.3308951071402859 1109 24-25 -0.18418141533422983 1109 26-27 0.6587384742229219 1109 28-29 0.29978797577670946 1109 30-31 0.21968416698707216 1109 32-33 0.10551520680147064 1109 34-35 0.12047227126701898 1109 36-37 0.19548608641139964 1109 38-39 0.6435592913814858 1109 40-41 0.1992310556316923 1109 42-43 0.17353910071312129 1109 44-45 0.41028654563060485 1109 46-47 0.2497340113086537 1109 48-49 0.11731224421922803 1109 50-51 0.26012400783191225 1109 52-53 0.32698219622997016 1109 54-55 0.4057868806729985 1109 56-57 0.17285323732850344 1109 58-59 0.2052100985634837 1109 60-61 -0.20110635915594344 1109 62-63 0.361965665525652 1109 64-65 0.29611778464010996 1109 66-67 0.7632058589469324 1109 68-69 0.5236906874334153 1109 70-71 0.6070799183131186 1109 72-73 -0.057483536234840926 1109 74-75 0.08924418243442744 1109 76 0.47317335176921915 1110 1 -0.20360886303432935 1110 2 -0.069060041971575 1110 3 0.37016534104429866 1110 4 -0.06993292625397984 1110 5 -0.11141720697629864 1110 6 0.2535839639803612 1110 7 0.05237785700056463 1110 8 0.08141721794925871 1110 9 -0.10040730323006386 1110 10-11 0.0014186514288780927 1110 12-13 0.5000560870868753 1110 14-15 0.14669262668402894 1110 16-17 0.24514900620685864 1110 18-19 0.023896629561747318 1110 20-21 0.12874047416404721 1110 22-23 0.34815987468252274 1110 24-25 -0.06215650474106127 1110 26-27 0.005945281474126318 1110 28-29 0.3070330121560403 1110 30-31 0.19594511161648853 1110 32-33 0.3717625862563949 1110 34-35 0.29771360805330715 1110 36-37 0.6329937938969081 1110 38-39 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-0.317999895822652 2116 10-11 0.57453956646156 2116 12-13 0.4792227537535396 2116 14-15 0.3264311887755298 2116 16-17 0.21846055304781942 2116 18-19 0.5580741894745955 2116 20-21 0.3573624785213454 2116 22-23 0.12675464592435048 2116 24-25 0.058350031206657604 2116 26-27 0.26792785228022353 2116 28-29 0.31411362217782823 2116 30-31 -0.10321066180399185 2116 32-33 0.3138922158860211 2116 34-35 0.4017441091426335 2116 36-37 0.22422473071608096 2116 38-39 0.2688650216400461 2116 40-41 -0.2358231022998254 2116 42-43 0.26137103642241755 2116 44-45 0.5624528628912628 2116 46-47 0.22579969963849322 2116 48-49 0.6762308326189483 2116 50-51 0.26449158295420006 2116 52-53 0.17162754913004363 2116 54-55 0.26855767033057987 2116 56-57 -0.014777789925169316 2116 58-59 0.48604517892686516 2116 60-61 -0.061474982496264374 2116 62-63 -0.13838374048413016 2116 64-65 -0.5065988470859537 2116 66-67 0.28028307767509375 2116 68-69 0.5512500864550844 2116 70-71 0.5531403655528209 2116 72-73 0.5618822918574011 2116 74-75 0.5596320231052871 2116 76 0.37481556001520033 2117 1 0.3470834518062986 2117 2 0.6117317060072374 2117 3 0.0974521483422599 2117 4 0.6152574216071223 2117 5 -0.06916586726189777 2117 6 -0.0893984106440584 2117 7 0.3201193927934014 2117 8 0.14374104163354673 2117 9 -0.014046060470718658 2117 10-11 0.3496514674620883 2117 12-13 -0.09802184866879315 2117 14-15 -0.09313173908123673 2117 16-17 0.4684166703025099 2117 18-19 0.4474787006208203 2117 20-21 -0.3340650271834136 2117 22-23 0.002807831811665551 2117 24-25 -0.057270031984494096 2117 26-27 0.5028321878954642 2117 28-29 0.11997635695051656 2117 30-31 0.09082786724638225 2117 32-33 -0.15061761384892236 2117 34-35 0.3593094944331341 2117 36-37 0.30740447444083685 2117 38-39 0.2550529275554325 2117 40-41 -0.09483881232264224 2117 42-43 0.5003893794099525 2117 44-45 0.2868253396334097 2117 46-47 0.4474075634264594 2117 48-49 0.27660164181676805 2117 50-51 0.2278056078796027 2117 52-53 0.3487712212381737 2117 54-55 0.40022784761687547 2117 56-57 -0.16340864827166257 2117 58-59 0.04092055193019917 2117 60-61 0.28823758552199763 2117 62-63 0.11639947875406165 2117 64-65 0.32520956084804453 2117 66-67 0.26930142066262874 2117 68-69 -0.038062270925991015 2117 70-71 0.5251102619895391 2117 72-73 -0.7225108097750379 2117 74-75 0.31838663079354745 2117 76 -0.3578946383348054 2118 1 0.5576304393164442 2118 2 0.9286650149020019 2118 3 0.8756721478185732 2118 4 0.3111019923175 2118 5 1.0929665723181046 2118 6 1.0511320808329927 2118 7 0.8910916113789327 2118 8 1.0700063921087946 2118 9 0.9468609595078163 2118 10-11 0.565369830427187 2118 12-13 1.183143871003388 2118 14-15 0.9109922691929597 2118 16-17 1.0261135098720473 2118 18-19 0.6915021616456656 2118 20-21 0.9168186888753453 2118 22-23 0.7396306434688569 2118 24-25 1.0734283000832647 2118 26-27 0.5936389224664325 2118 28-29 0.8155459937759488 2118 30-31 0.6348827492866533 2118 32-33 0.47366559423674914 2118 34-35 0.5167942421485634 2118 36-37 0.8670782195640427 2118 38-39 0.9738348395242227 2118 40-41 0.8290045015471108 2118 42-43 0.8140234816853535 2118 44-45 0.8436581012788196 2118 46-47 0.755163317399905 2118 48-49 0.7991462203823971 2118 50-51 0.5590485708813588 2118 52-53 0.7359435916919423 2118 54-55 0.6275458021087701 2118 56-57 0.9850687416893251 2118 58-59 0.8343928359761748 2118 60-61 0.3917043708902739 2118 62-63 0.7445710374982681 2118 64-65 0.5159482165522675 2118 66-67 0.7072449152032263 2118 68-69 0.46056305964927446 2118 70-71 0.7194361502878337 2118 72-73 0.7964203520170088 2118 74-75 0.4749942084950973 2118 76 1.3349649359104347 2119 1 0.9044023414474616 2119 2 1.2748055042469133 2119 3 0.41647719912086245 2119 4 1.113185654511657 2119 5 1.466920794891113 2119 6 1.4198771479206016 2119 7 1.421920340660698 2119 8 1.9333686651948199 2119 9 1.432291109468352 2119 10-11 1.3162459156679063 2119 12-13 1.6218021188329033 2119 14-15 1.5634153157596558 2119 16-17 1.6703124048996685 2119 18-19 1.1166323905328 2119 20-21 1.0927460764049428 2119 22-23 1.1973102014799082 2119 24-25 1.817516697873323 2119 26-27 1.740679175031545 2119 28-29 1.4152776433418524 2119 30-31 1.1540776979843699 2119 32-33 1.596207030700839 2119 34-35 1.863913421311949 2119 36-37 1.7340395455308908 2119 38-39 1.1831639634389859 2119 40-41 1.5774970035202784 2119 42-43 1.2605112480626275 2119 44-45 1.1625057729441721 2119 46-47 1.1539478477866467 2119 48-49 1.3634398273279373 2119 50-51 1.3069254453880674 2119 52-53 1.2418788718813687 2119 54-55 1.4263619031348114 2119 56-57 1.2945872894399173 2119 58-59 1.4988758667575581 2119 60-61 0.9692260757047961 2119 62-63 1.0869072648068752 2119 64-65 1.0768006238135186 2119 66-67 1.195137575029591 2119 68-69 1.1002315679365609 2119 70-71 1.0788678787803292 2119 72-73 1.8004140378891407 2119 74-75 1.5601875786608446 2119 76 1.7580430732427175 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 16.0 13 180.0 14 756.0 15 1322.0 16 1668.0 17 1760.0 18 1595.0 19 1338.0 20 991.0 21 769.0 22 542.0 23 508.0 24 498.0 25 569.0 26 673.0 27 742.0 28 955.0 29 1205.0 30 1521.0 31 2054.0 32 2594.0 33 3663.0 34 5287.0 35 8436.0 36 16851.0 37 52433.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.211225969924534 18.418008556267555 10.289554376365606 29.0812110974423 2 22.23619705120908 19.88781374511136 29.242788682224628 28.63320052145493 3 20.219231404806933 21.01151240291574 27.999742944751482 30.769513247525843 4 15.79788113030865 17.574316508455283 32.52850559095165 34.09929677028442 5 14.497916016378094 31.90422855883811 32.55604722472137 21.041808200062427 6 28.761074133578152 34.75235253614873 18.128069772779433 18.358503557493687 7 28.402768852248318 29.38692323228614 20.145787048087694 22.064520867377855 8 25.22354626076419 31.39195417072141 20.527697703027744 22.856801865486663 9 24.472577713309953 18.02049097552467 20.277986890182326 37.22894442098305 10-11 25.498503571231844 23.94974569891486 25.776674072305973 24.775076657547327 12-13 24.898095955052053 21.69454492040468 25.49529038062538 27.91206874391789 14-15 23.78311881460808 22.749389493784772 23.179498007821824 30.287993683785324 16-17 24.49966031985017 25.1739713199787 24.412445146246075 25.91392321392505 18-19 23.314911040522922 24.827405761709784 25.27633439215614 26.58134880561115 20-21 23.269008317573398 25.01285276242587 25.518700769329637 26.1994381506711 22-23 24.73514128858124 24.462938141490554 25.11888805243927 25.683032517488936 24-25 23.842792354442466 25.491159135559922 24.752584323302056 25.913464186695556 26-27 22.84899840258524 25.650441584194773 25.7349025944219 25.76565741879808 28-29 23.472816407469292 24.95088408644401 26.94673448028937 24.62956502579733 30-31 23.7620035620513 24.461102032572573 26.202651341277566 25.57424306409856 32-33 22.681453463819473 25.53843894019793 26.335769237831187 25.44433835815141 34-35 23.214843104492957 25.59076804436039 26.11864935827993 25.075739492866717 36-37 23.416815085470873 24.565301213667993 26.021794612856436 25.996089088004698 38-39 22.64794447606632 24.570350513192444 27.55494555937058 25.226759451370658 40-41 22.609949001840707 23.78506410344685 28.04669246411538 25.558294430597062 42-43 21.490736830508787 23.56370379890935 28.04931788553697 26.896241485044893 44-45 20.917870848098712 23.32041936727687 29.446137744891026 26.315572039733397 46-47 20.66402879018783 23.491636523878594 29.885426803517984 25.958907882415584 48-49 20.315627123000937 24.32706608155996 29.637552099590547 25.719754695848557 50-51 20.085654481023816 24.914620935313884 28.80441767805666 26.19530690560564 52-53 19.365899785175255 25.245579567779963 28.721792776747517 26.66672787029727 54-55 19.105172318821953 24.954097277050476 28.538181884949417 27.40254851917816 56-57 19.034482125479684 25.00367221783596 28.439950057837432 27.521895598846925 58-59 19.47606632025412 24.65664763233755 28.02912068743918 27.838165359969153 60-61 19.700989662706792 24.264179351119108 28.22650239612214 27.80832859005196 62-63 19.945192148798267 24.44090483447478 27.65501349540055 27.95888952132641 64-65 20.856820226575838 23.896039513063915 26.347703945798063 28.899436314562184 66-67 20.65668435451591 24.36929658667352 25.695885279914805 29.278133778895764 68-69 21.074858160586086 24.26096616051264 25.337385013679008 29.326790665222262 70-71 21.709233791748527 23.67111617061124 25.719295668619065 28.90035436902117 72-73 22.114554835392834 23.3268457484898 25.94651414721921 28.612085268898152 74-75 22.450103740153864 23.246975010557627 26.041073756495237 28.261847492793272 76 22.281181719699614 22.42348016084314 26.670400088133228 28.624938031324014 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.5 14 2.0 15 2.0 16 8.5 17 15.0 18 15.0 19 18.5 20 21.0 21 20.0 22 30.5 23 63.0 24 97.5 25 110.0 26 179.0 27 279.0 28 377.5 29 445.0 30 516.5 31 742.0 32 1094.0 33 1292.0 34 1526.0 35 2019.5 36 2432.0 37 2585.0 38 3004.5 39 3853.5 40 4720.5 41 5375.0 42 5592.0 43 5822.0 44 6294.5 45 6521.5 46 6506.0 47 6649.0 48 6604.5 49 6156.0 50 5895.0 51 5495.5 52 4677.5 53 3865.0 54 3471.0 55 3340.5 56 2956.0 57 2582.0 58 2462.0 59 2310.5 60 2122.0 61 2088.5 62 2092.0 63 2112.5 64 2060.0 65 2026.5 66 1971.5 67 1877.0 68 1794.5 69 1540.5 70 1238.0 71 1107.0 72 919.0 73 622.0 74 385.0 75 257.0 76 182.0 77 79.0 78 34.5 79 18.0 80 13.5 81 9.0 82 9.5 83 10.0 84 8.0 85 4.0 86 3.5 87 5.0 88 4.0 89 3.0 90 2.0 91 1.5 92 2.0 93 2.0 94 2.5 95 2.0 96 1.0 97 2.0 98 4.5 99 14.5 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 9.180544589905072E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 4.590272294952536E-4 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 108926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.05153957732773 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9447376731177 45.75491618162789 2 13.378746184267905 16.335861043277085 3 4.712711087051322 8.63154802342875 4 2.26011638922723 5.51934340745093 5 1.3187771612456956 4.0256688026733745 6 0.8736710726154494 3.2003378440409085 7 0.5443527165005038 2.3263499990819456 8 0.44510608863024614 2.1739529588895214 9 0.3774379332641614 2.073885022859556 >10 1.1428399572938752 9.911315939261517 >50 0.0015037367859129936 0.04682077740851587 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0036722178359620294 0.0 2 0.0 0.0 0.0 0.004590272294952537 0.0 3 0.0 0.0 0.0 0.004590272294952537 0.0 4 0.0 0.0 0.0 0.007344435671924059 0.0 5 0.0 0.0 0.0 0.008262490130914566 0.0 6 0.0 0.0 0.0 0.008262490130914566 0.0 7 0.0 0.0 0.0 0.008262490130914566 0.0 8 0.0 0.0 0.0 0.009180544589905073 0.0 9 0.0 0.0 0.0 0.011934707966876595 0.0 10 0.0 0.0 0.0 0.013770816884857609 0.0 11 0.0 0.0 0.0 0.014688871343848118 0.0 12 0.0 0.0 0.0 0.015606925802838624 0.0 13 0.0 0.0 0.0 0.01744303472081964 0.0 14 0.0 0.0 0.0 0.02019719809779116 0.0 15 0.0 0.0 0.0 0.02019719809779116 0.0 16 0.0 0.0 0.0 0.02019719809779116 0.0 17 0.0 0.0 0.0 0.022951361474762684 0.0 18 0.0 0.0 0.0 0.027541633769715218 0.0 19 0.0 0.0 0.0 0.035804123900629786 0.0 20 0.0 0.0 0.0 0.03855828727760131 0.0 21 0.0 0.0 0.0 0.04314855957255384 0.0 22 0.0 0.0 0.0 0.046820777408515875 0.0 23 0.0 0.0 0.0 0.0504929952444779 0.0 24 0.0 0.0 0.0 0.05783743091640196 0.0 25 0.0 0.0 0.0 0.05967353983438298 0.0 26 0.0 0.0 0.0 0.07252630226025007 0.0 27 0.0 0.0 0.0 0.12760956979968052 0.0 28 0.0 0.0 0.0 0.2010539265189211 0.0 29 0.0 0.0 0.0 0.26990801094320915 0.0 30 0.0 0.0 0.0 0.33784404090850667 0.0 31 0.0 0.0 0.0 0.43148559572553846 0.0 32 0.0 0.0 0.0 0.5673576556561335 0.0 33 0.0 0.0 0.0 0.6903769531608615 0.0 34 0.0 0.0 0.0 0.8537906468611718 0.0 35 0.0 0.0 0.0 1.0704514991829315 0.0 36 0.0 0.0 0.0 1.4771496245157263 0.0 37 0.0 0.0 0.0 2.086737785285423 0.0 38 0.0 0.0 0.0 2.7660980849383985 0.0 39 0.0 0.0 0.0 3.5537888107522537 0.0 40 0.0 0.0 0.0 4.422268328957274 0.0 41 0.0 0.0 0.0 5.553311422433579 0.0 42 0.0 0.0 0.0 6.867047353248995 0.0 43 0.0 0.0 0.0 8.277179002258414 0.0 44 0.0 0.0 0.0 9.431173457209482 0.0 45 0.0 0.0 0.0 10.525494372326166 0.0 46 0.0 0.0 0.0 11.65286524796651 0.0 47 0.0 0.0 0.0 12.780236123606853 0.0 48 0.0 0.0 0.0 13.619337899124176 0.0 49 0.0 0.0 0.0 14.472210491526358 0.0 50 0.0 0.0 0.0 15.41780658428658 0.0 51 0.0 0.0 0.0 16.451535905109893 0.0 52 0.0 0.0 0.0 17.552283201439508 0.0 53 0.0 0.0 0.0 18.83755944402622 0.0 54 0.0 0.0 0.0 20.011751097075077 0.0 55 0.0 0.0 0.0 20.928887501606596 0.0 56 0.0 0.0 0.0 21.745037915649156 0.0 57 0.0 0.0 0.0 22.570368874281623 0.0 58 0.0 0.0 0.0 23.318583258358885 0.0 59 0.0 0.0 0.0 24.06771569689514 0.0 60 0.0 0.0 0.0 24.77369957585884 0.0 61 0.0 0.0 0.0 25.48151956374052 0.0 62 0.0 0.0 0.0 26.230652002276774 0.0 63 0.0 0.0 0.0 27.065163505499147 0.0 64 0.0 0.0 0.0 27.963020766391864 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACTTT 20 0.0069352346 52.5 33 CGCATCT 20 0.0069352346 52.5 24 TAGAACC 20 0.0069352346 52.5 65 TCGAGAT 20 0.0069352346 52.5 9 GCATACT 20 0.0069352346 52.5 66 TTTGATA 35 0.0012507705 40.0 37 CGGACTA 35 0.0012507705 40.0 24 AAGAGGT 45 9.1471404E-5 38.888885 16 ACATGTC 40 0.0024098614 35.0 20 ATTACTT 45 0.0042915205 31.111109 8 TTATGCT 45 0.0042915205 31.111109 19 CAGATAA 45 0.0042915205 31.111109 14 GCAGATA 45 0.0042915205 31.111109 13 CTAATAG 45 0.0042915205 31.111109 57 TAATAGC 60 4.955074E-4 29.166666 58 ACACTCT 60 4.955074E-4 29.166666 6 AATCCTC 50 0.0071821897 27.999998 23 CGGCGGA 50 0.0071821897 27.999998 21 GGCAGAT 50 0.0071821897 27.999998 12 AAAGTTA 50 0.0071821897 27.999998 52 >>END_MODULE