FastQCFastQC Report
Wed 25 May 2016
SRR1780244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780244_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108926
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT42473.8989772873326847No Hit
CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG16001.4688871343848118RNA PCR Primer, Index 18 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG10720.9841543800378239No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC8300.7619852009621211No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC3370.30938435267980097No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT1920.1762664561261774RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT1580.14505260452050014No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT1350.12393735196371848No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1120.1028220994069368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAA207.8964826E-570.067
CGGAATA252.3814049E-455.99999669
TCGGAAT252.3814049E-455.99999668
TTGCACT252.3814049E-455.99999627
GGCACCC200.006935234652.512
GTAGACT200.006935234652.58
GATTCTT200.006935234652.51
CGGAACT352.0749314E-550.034
AACTCGT352.0749314E-550.037
TCTTTAT651.8189894E-1048.461541
CTTCGGA305.85586E-446.66666466
TACCAGG305.85586E-446.66666459
CTTTATA703.783498E-1045.02
GAGACAC350.001250770540.09
GCTTCGG350.001250770540.065
CTACCAG350.001250770540.058
TGAAGTT459.1471404E-538.88888539
TTTCGGT459.1471404E-538.88888527
TTCGGTC459.1471404E-538.88888528
TTTTCGG459.1471404E-538.88888526