##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780244_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 108926 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.56443824247654 34.0 34.0 34.0 34.0 34.0 2 33.59566127462681 34.0 34.0 34.0 34.0 34.0 3 33.655013495400546 34.0 34.0 34.0 34.0 34.0 4 33.67019811615225 34.0 34.0 34.0 34.0 34.0 5 33.64190367772616 34.0 34.0 34.0 34.0 34.0 6 37.305354093604834 38.0 38.0 38.0 36.0 38.0 7 37.289765528891174 38.0 38.0 38.0 36.0 38.0 8 37.18749426215963 38.0 38.0 38.0 36.0 38.0 9 37.212208288195654 38.0 38.0 38.0 36.0 38.0 10-11 37.169477443401945 38.0 38.0 38.0 36.0 38.0 12-13 37.14921598149202 38.0 38.0 38.0 36.0 38.0 14-15 37.06272148063823 38.0 38.0 38.0 36.0 38.0 16-17 37.10873896039513 38.0 38.0 38.0 36.0 38.0 18-19 37.079173016543336 38.0 38.0 38.0 35.5 38.0 20-21 37.09968694342949 38.0 38.0 38.0 36.0 38.0 22-23 37.058498430126875 38.0 38.0 38.0 35.5 38.0 24-25 37.100224005288 38.0 38.0 38.0 36.0 38.0 26-27 36.96129941428126 38.0 37.5 38.0 35.5 38.0 28-29 36.905073168940376 38.0 37.5 38.0 35.0 38.0 30-31 36.90791454749096 38.0 37.0 38.0 35.0 38.0 32-33 36.815402199658486 38.0 37.0 38.0 35.0 38.0 34-35 36.86497714044397 38.0 37.0 38.0 35.0 38.0 36-37 36.849333492462776 38.0 37.0 38.0 35.0 38.0 38-39 36.305661641848594 38.0 37.0 38.0 34.0 38.0 40-41 36.58150487486918 38.0 37.0 38.0 34.0 38.0 42-43 36.752685309292545 38.0 37.0 38.0 34.0 38.0 44-45 36.83382296237812 38.0 37.0 38.0 35.0 38.0 46-47 36.751450526045204 38.0 37.0 38.0 34.5 38.0 48-49 36.8007454602207 38.0 37.0 38.0 34.5 38.0 50-51 36.61238363659732 38.0 37.0 38.0 34.0 38.0 52-53 36.65001009859905 38.0 37.0 38.0 34.0 38.0 54-55 36.60395589666379 38.0 37.0 38.0 34.0 38.0 56-57 36.65031305657052 38.0 37.0 38.0 34.0 38.0 58-59 36.53571231845473 38.0 37.0 38.0 34.0 38.0 60-61 36.543300038558286 38.0 37.0 38.0 34.0 38.0 62-63 36.514284927381894 38.0 37.0 38.0 34.0 38.0 64-65 36.439454308429575 38.0 37.0 38.0 34.0 38.0 66-67 36.13210344637643 38.0 37.0 38.0 33.0 38.0 68-69 36.10542019352588 38.0 37.0 38.0 34.0 38.0 70-71 35.633999228834256 38.0 37.0 38.0 30.5 38.0 72-73 34.941171070267885 38.0 36.5 38.0 25.5 38.0 74-75 34.72404660044434 38.0 36.0 38.0 24.0 38.0 76 33.905605640526595 38.0 34.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06229241459759294 1101 2 0.14022531533085214 1101 3 0.1556133806661819 1101 4 -0.012552365512554786 1101 5 0.020555530552130108 1101 6 0.012397148292272675 1101 7 0.1419363431236036 1101 8 0.18926071028384683 1101 9 0.14645112605241195 1101 10-11 0.31502962314927174 1101 12-13 0.09995291201033751 1101 14-15 0.3966330640935851 1101 16-17 0.21431102248043743 1101 18-19 0.1959066548633146 1101 20-21 0.10231748615986902 1101 22-23 0.1775842133634029 1101 24-25 0.2050241973279867 1101 26-27 0.2643548082912446 1101 28-29 -0.2776499377120629 1101 30-31 0.11105690910905253 1101 32-33 -0.07881152999177488 1101 34-35 0.07893702618966358 1101 36-37 0.0839479434832171 1101 38-39 0.43308424422490077 1101 40-41 0.24266768873347644 1101 42-43 0.26371397714316913 1101 44-45 0.23934319716136088 1101 46-47 0.22670219783785228 1101 48-49 0.2052390857781745 1101 50-51 -0.07513335114057185 1101 52-53 0.002332677250386439 1101 54-55 -0.08834021772624823 1101 56-57 -0.10305985799026729 1101 58-59 0.049894206009732045 1101 60-61 0.04790510756981803 1101 62-63 0.01925373201056857 1101 64-65 0.08509660996188728 1101 66-67 -0.33527876402068557 1101 68-69 -0.26406888809648166 1101 70-71 -0.21072543986489478 1101 72-73 -0.061015440062114124 1101 74-75 0.21454867383429388 1101 76 -0.7794153265982047 1102 1 0.024219559133427992 1102 2 -0.043218998548248067 1102 3 -0.22341782445198533 1102 4 -0.313583831761413 1102 5 -0.21143297969724273 1102 6 0.025244778531202883 1102 7 -0.10321315419980692 1102 8 -0.045521898411806205 1102 9 0.03507888424538663 1102 10-11 -0.1680229946859484 1102 12-13 0.1678599494825903 1102 14-15 0.21472942132037787 1102 16-17 0.00651627120742404 1102 18-19 0.036669156495378274 1102 20-21 0.0942485476561501 1102 22-23 0.08658016583761707 1102 24-25 -0.09624228654718792 1102 26-27 0.24473083649335337 1102 28-29 0.1316985412016365 1102 30-31 0.08709816775900947 1102 32-33 -0.016649367829607797 1102 34-35 0.1964197262810572 1102 36-37 0.062404661070374345 1102 38-39 -0.18022036473188052 1102 40-41 0.3749956688875429 1102 42-43 0.2419879032431851 1102 44-45 0.3114936084422055 1102 46-47 0.033347974048844264 1102 48-49 0.03946026347760778 1102 50-51 0.2343456931210781 1102 52-53 0.21706044849989325 1102 54-55 0.0754931808897581 1102 56-57 0.037167325715174115 1102 58-59 0.24474993130024103 1102 60-61 0.10069267774869672 1102 62-63 0.21467088829728453 1102 64-65 0.4961946645382014 1102 66-67 0.4048178543368053 1102 68-69 0.45861031284358234 1102 70-71 0.271961594996057 1102 72-73 0.011383045379851353 1102 74-75 0.03795904072291734 1102 76 -0.012265568144101735 1103 1 0.017405422342179122 1103 2 0.271172051540745 1103 3 0.12834927445470612 1103 4 -0.13796725408533916 1103 5 0.18129521760761236 1103 6 0.054281137544684555 1103 7 -0.01137486281918143 1103 8 -0.05342934819568512 1103 9 -0.09205053350187598 1103 10-11 0.1575300182812427 1103 12-13 0.33078901126748406 1103 14-15 0.2736284805626852 1103 16-17 0.17661543215327669 1103 18-19 0.2976531381887497 1103 20-21 0.3147799486223377 1103 22-23 0.2980599522601963 1103 24-25 0.23951921866511583 1103 26-27 0.03715392319561772 1103 28-29 0.21041707667074405 1103 30-31 0.16566414792681883 1103 32-33 0.425455895328291 1103 34-35 0.06913775323248927 1103 36-37 0.3520054746986503 1103 38-39 0.2516001285302636 1103 40-41 0.1355474105605623 1103 42-43 0.07037590222614654 1103 44-45 0.10752717060849193 1103 46-47 0.21528288378696203 1103 48-49 0.26000437738557025 1103 50-51 -0.016061121015717106 1103 52-53 0.09827072055684738 1103 54-55 0.22567116207460458 1103 56-57 0.0074190419623150206 1103 58-59 0.2514115127901988 1103 60-61 0.2885009665867315 1103 62-63 0.27565486999064603 1103 64-65 0.5240487199666362 1103 66-67 0.31530603130095614 1103 68-69 0.14599907460305417 1103 70-71 -0.05496441568281085 1103 72-73 -0.2361379309740812 1103 74-75 -0.4464676056030399 1103 76 0.2498244394836604 1104 1 0.1589159503146078 1104 2 0.021772584980098486 1104 3 0.06701203144159962 1104 4 0.17698561439369342 1104 5 0.15258557759634783 1104 6 -0.04693980906168349 1104 7 0.012679607634574097 1104 8 0.059893382616230895 1104 9 0.26343212971897145 1104 10-11 0.2552885789889672 1104 12-13 0.233229247836249 1104 14-15 0.26652561953854814 1104 16-17 0.2148342085560344 1104 18-19 0.18912225006191363 1104 20-21 0.08964617462170565 1104 22-23 0.20876270063254054 1104 24-25 -0.036887686226954486 1104 26-27 0.15590216301140458 1104 28-29 -0.2317329158743604 1104 30-31 -0.02948022859522581 1104 32-33 0.13103712886538688 1104 34-35 0.10599808157932955 1104 36-37 0.1281763078248943 1104 38-39 -0.39973015657815836 1104 40-41 0.028093341014653106 1104 42-43 0.21227159992175615 1104 44-45 0.1382661994837946 1104 46-47 0.07747081347898899 1104 48-49 0.17269095691275993 1104 50-51 0.0507886048097177 1104 52-53 -0.006255230770946696 1104 54-55 -0.18643973367278477 1104 56-57 -0.05594758991482962 1104 58-59 0.07031547713921782 1104 60-61 0.09186975210104009 1104 62-63 0.023592348247191808 1104 64-65 0.07626199631140906 1104 66-67 -0.4626001363845518 1104 68-69 -0.2785904918532154 1104 70-71 -0.6605506191518273 1104 72-73 -0.42514444158488374 1104 74-75 -0.7515675840666702 1104 76 -0.7998748796356665 1105 1 0.04241046368115775 1105 2 0.21677600395049978 1105 3 0.20386853236960434 1105 4 0.09428723270634976 1105 5 0.13249505628476044 1105 6 0.16337571306394238 1105 7 0.1657273755353259 1105 8 -0.057865726497766445 1105 9 0.3784072200774702 1105 10-11 0.2548530672830651 1105 12-13 0.40716529680892677 1105 14-15 0.20569643781212932 1105 16-17 0.23590157855375793 1105 18-19 0.27731133540592623 1105 20-21 0.2970596421089269 1105 22-23 0.15317186998554178 1105 24-25 0.32378519970968256 1105 26-27 0.43148745818251655 1105 28-29 0.41927975059693523 1105 30-31 0.350404014835469 1105 32-33 0.334787413779587 1105 34-35 0.3358749986448757 1105 36-37 0.21888891894143825 1105 38-39 -0.15919337822512603 1105 40-41 0.011177577933018767 1105 42-43 0.2281698122886553 1105 44-45 0.31510430309488413 1105 46-47 -0.08941564414205772 1105 48-49 0.005189898660020731 1105 50-51 -0.25232347229621155 1105 52-53 0.14558368688070544 1105 54-55 -0.008877133952822192 1105 56-57 0.27329925974815694 1105 58-59 0.30573194029574324 1105 60-61 0.20060522147682747 1105 62-63 0.30129007870208113 1105 64-65 0.10675188592751539 1105 66-67 0.1465220022628344 1105 68-69 0.5618087136431811 1105 70-71 0.31738888135287624 1105 72-73 -0.2611681790079601 1105 74-75 -0.46010442754295156 1105 76 -0.49972184000817066 1106 1 0.26479926927835606 1106 2 0.10170853768363486 1106 3 -2.294186548823518E-4 1106 4 -0.01793165784837214 1106 5 -0.002737327523334443 1106 6 0.10350564809642293 1106 7 0.07359844000309579 1106 8 0.20375346390703442 1106 9 0.4495716378685799 1106 10-11 0.31313642560390775 1106 12-13 0.26061357267099794 1106 14-15 0.12487434885660775 1106 16-17 0.20506699584510812 1106 18-19 0.2091329246113247 1106 20-21 0.18700217084455772 1106 22-23 -0.016318384887021864 1106 24-25 0.06172872794556383 1106 26-27 0.05777431475422645 1106 28-29 0.10561158467989884 1106 30-31 0.1899967184836413 1106 32-33 0.16451005246024408 1106 34-35 -0.00937737516822068 1106 36-37 0.14211480599790605 1106 38-39 -0.1918145676304306 1106 40-41 0.24745943700348505 1106 42-43 0.1376400771562203 1106 44-45 0.14627621713786 1106 46-47 0.4449421769473929 1106 48-49 0.448155915651526 1106 50-51 0.19231670761383413 1106 52-53 0.2982198687897508 1106 54-55 0.41462361567236883 1106 56-57 0.36180500687459727 1106 58-59 0.13750355448865292 1106 60-61 0.12388108354579686 1106 62-63 0.4581491491668501 1106 64-65 0.41068741816139465 1106 66-67 0.43380336158318045 1106 68-69 0.2904943708145993 1106 70-71 0.015439855865629681 1106 72-73 -0.16108072273609508 1106 74-75 -0.1374032781176595 1106 76 0.1877344318559011 1107 1 0.00769782000299557 1107 2 -0.04189394678841296 1107 3 0.027513852566649177 1107 4 0.1842215512613592 1107 5 0.10517157516817832 1107 6 0.06081413670926139 1107 7 0.15484067602793772 1107 8 0.33878451980765334 1107 9 0.269737269338556 1107 10-11 0.3284573365769887 1107 12-13 0.2744230964805183 1107 14-15 0.26880809626861435 1107 16-17 0.36140239957181564 1107 18-19 0.18116191011857552 1107 20-21 0.1108116946540818 1107 22-23 0.37359879937798723 1107 24-25 0.14170802401181248 1107 26-27 0.07230847624490622 1107 28-29 0.47064264058051464 1107 30-31 0.15200500005714446 1107 32-33 0.3100172988370602 1107 34-35 0.153707510960146 1107 36-37 0.4345785741138499 1107 38-39 0.11748149862216195 1107 40-41 0.30114473721052093 1107 42-43 0.29575601918521244 1107 44-45 0.2846199024380667 1107 46-47 0.069911121046772 1107 48-49 0.005050325589408544 1107 50-51 0.1273891713819495 1107 52-53 0.16004181495952707 1107 54-55 0.38234618296014844 1107 56-57 0.1656145306841168 1107 58-59 0.2781867843023136 1107 60-61 -0.010404630739920151 1107 62-63 0.12154459430142595 1107 64-65 0.6270228218881044 1107 66-67 0.6410704423285196 1107 68-69 0.2910533770257757 1107 70-71 0.1677172885778404 1107 72-73 0.2421076830610076 1107 74-75 -0.20168899240336913 1107 76 -0.07338358056646399 1108 1 -0.16403870694310996 1108 2 0.24893582224494537 1108 3 0.20108100397811768 1108 4 0.024409746299852486 1108 5 0.09040681896629366 1108 6 -0.18909176577149367 1108 7 0.1018723270116837 1108 8 0.33197528114212815 1108 9 0.13362093058749025 1108 10-11 0.14607923563601588 1108 12-13 -0.009845509165330668 1108 14-15 0.12897918266724417 1108 16-17 0.1940542387506028 1108 18-19 0.03349723945989069 1108 20-21 -0.03136110260853542 1108 22-23 -0.013133450408155056 1108 24-25 0.18333334479881813 1108 26-27 -0.06255893179614702 1108 28-29 -0.04880132862393083 1108 30-31 -0.07750740370676112 1108 32-33 -0.07932401852821158 1108 34-35 0.056864055014891335 1108 36-37 0.22056659806595036 1108 38-39 0.16759270478127064 1108 40-41 0.21201727784354318 1108 42-43 0.0587500311612601 1108 44-45 0.1375198168948728 1108 46-47 0.04725928186798711 1108 48-49 0.27118377298034346 1108 50-51 0.10756881210051716 1108 52-53 -0.045800785553737455 1108 54-55 0.22466440366160612 1108 56-57 0.41050760168497646 1108 58-59 0.2817368270739067 1108 60-61 0.1511265925278309 1108 62-63 -0.04488825428711607 1108 64-65 0.24847705627646377 1108 66-67 0.24489422333792987 1108 68-69 -0.13025543392375027 1108 70-71 0.11587810979255408 1108 72-73 0.027676545336454694 1108 74-75 0.310500913499105 1108 76 0.6274376341469718 1109 1 0.14694310302186153 1109 2 0.15079248627537822 1109 3 -0.04813754006587345 1109 4 -0.07286739752570526 1109 5 0.12447076654284217 1109 6 -0.24716429994842315 1109 7 0.15678137683656246 1109 8 0.05089593040963081 1109 9 0.18506521183631008 1109 10-11 0.1560404452921702 1109 12-13 0.28577080537539956 1109 14-15 0.18105427776007588 1109 16-17 0.20797921897882787 1109 18-19 0.24518706735127438 1109 20-21 0.21624745386342425 1109 22-23 0.018765016808352186 1109 24-25 -0.04129287446077967 1109 26-27 0.11447379520268441 1109 28-29 0.06344587508186095 1109 30-31 0.1588783154210276 1109 32-33 1.72988370579219E-5 1109 34-35 0.33549547142735037 1109 36-37 0.05420121562328717 1109 38-39 0.016771705270855364 1109 40-41 0.2192669294836591 1109 42-43 0.04317740283300964 1109 44-45 0.2806486535611583 1109 46-47 0.2817619745957387 1109 48-49 0.07038725281313418 1109 50-51 0.3609111839605603 1109 52-53 0.30783157237192427 1109 54-55 0.0825618163833326 1109 56-57 0.4803584659940938 1109 58-59 0.16457492446404132 1109 60-61 0.15752888228793438 1109 62-63 -0.06297472285940131 1109 64-65 0.27995184255296834 1109 66-67 0.40476316470049056 1109 68-69 0.38717197540852766 1109 70-71 0.7522186362867345 1109 72-73 0.05424604784088416 1109 74-75 0.05347722502698815 1109 76 0.28242953713344576 1110 1 -0.2774002192126872 1110 2 -0.24654380673239018 1110 3 -0.030749452755479467 1110 4 0.06155114976743192 1110 5 0.11559174323540589 1110 6 -0.07901779606385162 1110 7 -0.017222816035555866 1110 8 0.02259404361717543 1110 9 0.08012008913313906 1110 10-11 -0.1764913095481333 1110 12-13 0.1478684746317782 1110 14-15 -0.09943653491719573 1110 16-17 0.17893741357742243 1110 18-19 0.1423383807068035 1110 20-21 0.29027014470076296 1110 22-23 0.14291719340226194 1110 24-25 0.1253121323218025 1110 26-27 0.11438130537656122 1110 28-29 -0.06116875064263638 1110 30-31 0.14364832405340877 1110 32-33 0.14707612236646384 1110 34-35 0.08401563420944314 1110 36-37 0.3877811884929372 1110 38-39 -0.002527838445999464 1110 40-41 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2116 1 0.013994116299294035 2116 2 0.10231240241793671 2116 3 -0.01931154425874837 2116 4 0.09205223909204818 2116 5 -0.061151176154574216 2116 6 -0.4052705193753994 2116 7 -0.4524080012207392 2116 8 -0.10240595638282457 2116 9 -0.14809341849213098 2116 10-11 0.010600193719845663 2116 12-13 -0.23012716806974254 2116 14-15 -0.048374443413926826 2116 16-17 -0.6857140025445858 2116 18-19 -0.06272697877031419 2116 20-21 -0.13197931063692891 2116 22-23 -0.1563747455955493 2116 24-25 -0.3637946214908325 2116 26-27 0.06127673021316582 2116 28-29 -0.05980709486922109 2116 30-31 0.21853939159153413 2116 32-33 -0.26618640486665157 2116 34-35 -0.06658349433086386 2116 36-37 -0.31551401847874416 2116 38-39 0.13391007005073163 2116 40-41 -0.2908658448000594 2116 42-43 -0.4141513882221943 2116 44-45 -0.12383247851431634 2116 46-47 -0.07991692128126715 2116 48-49 -0.09562427758518766 2116 50-51 -0.11742564343285977 2116 52-53 -0.40294760302989374 2116 54-55 -0.34109297047900355 2116 56-57 -0.3099542523846637 2116 58-59 -0.19522591411038803 2116 60-61 -0.42056336089864743 2116 62-63 -0.0314845062276774 2116 64-65 -0.581945432080154 2116 66-67 -0.4116676529095784 2116 68-69 -0.6398196388472357 2116 70-71 -0.29875096539041834 2116 72-73 -0.45641083545751115 2116 74-75 -0.42490327811766093 2116 76 -0.409044956227838 2117 1 -0.560738198954347 2117 2 -0.05283504360628655 2117 3 -0.6202857412045049 2117 4 0.02947544428776183 2117 5 -0.014043049070146196 2117 6 -0.07636934331782186 2117 7 -0.15887631787862944 2117 8 -0.48530485053763783 2117 9 -0.4374123582850018 2117 10-11 -0.2850159200242288 2117 12-13 -0.5893074383874506 2117 14-15 -0.41682494367722 2117 16-17 -0.6628291508682693 2117 18-19 -0.7289328766112817 2117 20-21 -0.5652885084603412 2117 22-23 -0.46050411192871366 2117 24-25 -0.7387726801181813 2117 26-27 -0.6215168453529287 2117 28-29 -1.1289443600965328 2117 30-31 -0.6277174102339842 2117 32-33 -0.7357646916842668 2117 34-35 -0.30054435092204557 2117 36-37 -0.4086441747013154 2117 38-39 -0.22450225222414844 2117 40-41 -0.9471761835748538 2117 42-43 -0.7108316585399095 2117 44-45 -0.6582914042647019 2117 46-47 -0.16931704415295457 2117 48-49 -0.2573870474640785 2117 50-51 -0.22085727411568 2117 52-53 -0.711684657620026 2117 54-55 -0.08508103437227987 2117 56-57 -0.6355093691127678 2117 58-59 -0.05021538225151545 2117 60-61 -0.6680620444162955 2117 62-63 -0.4349753786166559 2117 64-65 -0.7676352688363366 2117 66-67 -1.209159754015424 2117 68-69 -0.9613704550662945 2117 70-71 -0.6914905651797838 2117 72-73 -0.8549539283133996 2117 74-75 0.12752563183494914 2117 76 -0.032706532496050045 2118 1 0.2135541735941544 2118 2 -0.2300233864095631 2118 3 0.015832716023638227 2118 4 -0.28046668867549585 2118 5 -0.2734235313827327 2118 6 -0.3456736296680063 2118 7 -0.3278428283130239 2118 8 -0.06672455675114719 2118 9 -0.49795018133232105 2118 10-11 -0.28567052451219865 2118 12-13 -0.43459821370100826 2118 14-15 -0.394790956612205 2118 16-17 -0.11775308582676303 2118 18-19 -0.35823035507479517 2118 20-21 -0.446883648676625 2118 22-23 -0.4723917294302211 2118 24-25 -0.385024414820073 2118 26-27 -0.48008299968248025 2118 28-29 0.02795124357972867 2118 30-31 -0.2589263338023642 2118 32-33 -0.3439053530966376 2118 34-35 -0.8044850699396164 2118 36-37 -0.24122253085852918 2118 38-39 -0.3073882724907051 2118 40-41 -0.4116966203269641 2118 42-43 -0.42076331783135856 2118 44-45 -0.5597210604664298 2118 46-47 -0.3978394709027171 2118 48-49 -0.27476025057475795 2118 50-51 -0.23216883966777146 2118 52-53 -0.5079455567847546 2118 54-55 -0.1691060664479025 2118 56-57 -0.3063349562929929 2118 58-59 -0.0699189773393627 2118 60-61 -0.14186477280779997 2118 62-63 -0.23566140412004444 2118 64-65 -0.28229840936683104 2118 66-67 -0.32545678734788197 2118 68-69 -0.46816524965141326 2118 70-71 -0.2632614012406336 2118 72-73 -0.42684259318208717 2118 74-75 0.5056906445342761 2118 76 -0.22201815041650264 2119 1 -0.03515932285414891 2119 2 -0.07046537535984498 2119 3 0.07894242399522255 2119 4 0.04707869411850396 2119 5 -0.34625699626039363 2119 6 0.10938556528068943 2119 7 -0.0965878954006314 2119 8 -0.08121548019234837 2119 9 -0.1331198735185879 2119 10-11 -0.16868552056586594 2119 12-13 -0.3555769035194771 2119 14-15 -1.1911919037313865 2119 16-17 -0.39716902899960616 2119 18-19 0.205447624404286 2119 20-21 -0.15490259106019977 2119 22-23 -0.2564012006220153 2119 24-25 -0.3197205474167575 2119 26-27 -0.10197723804080283 2119 28-29 -0.09364307370520208 2119 30-31 -0.3351378570857122 2119 32-33 -0.5756969868772259 2119 34-35 -0.3391496318969942 2119 36-37 -0.2025642830290053 2119 38-39 0.37605292719358374 2119 40-41 0.059716165781949826 2119 42-43 -0.7342439808147887 2119 44-45 -0.47823724041907667 2119 46-47 -0.2700888789532243 2119 48-49 0.11076461130370063 2119 50-51 -0.038325114332330656 2119 52-53 -0.15424389932618965 2119 54-55 -0.27908238846842437 2119 56-57 -0.42867118360160106 2119 58-59 -0.7403846442691204 2119 60-61 -0.36754748788277425 2119 62-63 -0.27274111998428907 2119 64-65 -0.49726289239760746 2119 66-67 -0.5146438433857625 2119 68-69 -0.02466090868851012 2119 70-71 0.2548601457206914 2119 72-73 0.2963933973467263 2119 74-75 0.5454538647394784 2119 76 -0.9990978662807493 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 0.0 21 4.0 22 4.0 23 8.0 24 28.0 25 60.0 26 101.0 27 213.0 28 403.0 29 662.0 30 1080.0 31 1765.0 32 2642.0 33 4456.0 34 7079.0 35 10758.0 36 17208.0 37 62453.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.45682389879367 16.64616345041588 11.350825330958632 31.546187319831816 2 22.163670748948828 20.000734443567193 32.344894699153556 25.490700108330426 3 19.878633200521456 19.767548610983603 30.874171455850764 29.479646732644184 4 14.745790720305529 21.01702072966968 33.80643739786644 30.430751152158347 5 14.612672823751904 31.81334116739805 37.38684978792942 16.187136220920625 6 28.503754842737273 37.15733617318179 17.768025999302278 16.570882984778656 7 28.300864807300368 27.296513229164752 21.555000642638124 22.847621320896756 8 24.77553568477682 35.50024787470393 17.278702972660337 22.445513467858913 9 23.09182380698823 17.421001413803864 18.43912380882434 41.048050970383564 10-11 26.027761966839876 26.008482823201074 24.948588950296532 23.01516625966252 12-13 25.216201825092266 21.000495749407854 26.005269632594608 27.77803279290528 14-15 22.415217670712227 20.80081890457742 21.74549694287865 35.038466481831705 16-17 25.740869948405336 26.450985072434495 21.96583001303637 25.84231496612379 18-19 26.377999742944752 25.346565558268917 23.12946403980684 25.14597065897949 20-21 23.812955584525277 24.14391421699135 22.684666654425943 29.358463544057432 22-23 22.91877054146852 27.0210968914676 22.738372840276885 27.321759726786993 24-25 25.40807520702128 26.127829902869838 24.40234654719718 24.061748342911702 26-27 26.06907441749445 27.26116813249362 23.33923948368617 23.33051796632576 28-29 23.64816480913648 26.625415419642696 23.957090134586785 25.769329636634048 30-31 22.917393459780033 24.92150634375631 28.663496318601617 23.497603877862037 32-33 24.815471053742908 23.228613921377818 26.261406826652955 25.694508198226316 34-35 25.428272405119074 22.917393459780033 24.551989424012632 27.10234471108826 36-37 27.448451242127682 24.77874887538329 24.179259313662488 23.593540568826544 38-39 22.354626076418853 25.462699447331218 27.47782898481538 24.70484549143455 40-41 22.295411563813968 22.15173604098195 28.41194939683822 27.140902998365863 42-43 24.81317591759543 21.78038301232029 28.010300571029873 25.396140499054404 44-45 21.218992710647598 24.79894607348108 30.25907496832712 23.722986247544203 46-47 23.22172851293539 22.304133081174378 29.151901290784572 25.32223711510567 48-49 22.594238290215376 21.87035234930136 27.392449920129263 28.142959440354005 50-51 21.219910765106587 22.47994051007106 29.39839891302352 26.901749811798837 52-53 18.56122505187008 22.826047041110478 33.63567926849421 24.977048638525236 54-55 18.827460844977324 23.883186750638046 30.314617263096043 26.97473514128858 56-57 20.76088353561133 24.147127407597818 27.41081100930907 27.681178047481776 58-59 18.649358279933164 24.342673007362798 30.579935001744303 26.428033710959735 60-61 20.82881956557663 26.49000238694159 27.459926922865062 25.22125112461671 62-63 18.848576097534107 31.380478489984025 24.08699484053394 25.68395057194793 64-65 18.799460183978116 32.26502396122138 23.057396764776087 25.87811909002442 66-67 18.549749371132695 31.544351210913828 22.612599379395185 27.29330003855829 68-69 18.129739456144538 32.0111819033105 22.791160971668837 27.067917668876117 70-71 18.58142224996787 31.04355250353451 22.861851165011107 27.513174081486515 72-73 19.233699943080623 29.790408167012465 23.028478049317886 27.947413840589025 74-75 19.664726511576667 28.980225106953345 23.32133742173586 28.033710959734133 76 19.37737546591264 29.116097166883943 23.2396305748857 28.26689679231772 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 2.0 16 3.5 17 4.0 18 9.0 19 13.0 20 16.5 21 21.0 22 37.5 23 51.0 24 79.0 25 110.0 26 155.5 27 243.0 28 369.0 29 453.0 30 505.0 31 682.5 32 1017.0 33 1226.0 34 1408.0 35 1761.0 36 2109.0 37 2286.0 38 2676.0 39 3551.0 40 4557.5 41 6074.5 42 7070.0 43 7755.5 44 8470.0 45 8053.0 46 7607.0 47 7167.0 48 6376.5 49 5718.5 50 5411.0 51 4974.0 52 4134.0 53 3393.0 54 3055.0 55 2923.0 56 2541.5 57 2213.0 58 2134.0 59 1995.5 60 1876.0 61 1930.0 62 1965.0 63 1951.5 64 1922.5 65 1943.5 66 1830.0 67 1680.0 68 1657.0 69 1505.5 70 1180.0 71 983.0 72 869.0 73 628.5 74 356.5 75 211.0 76 152.5 77 58.0 78 16.5 79 11.0 80 7.5 81 3.0 82 1.0 83 0.0 84 0.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 108926.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.96559131887704 #Duplication Level Percentage of deduplicated Percentage of total 1 65.95785403946638 31.63707471127187 2 17.82877485788658 17.10335457099315 3 6.520948571209831 9.383434625341975 4 2.867150267000976 5.50098231827112 5 1.8297701303424119 4.388300313974625 6 1.2326066568415412 3.5473624295393207 7 0.8459815874595671 2.8404604961166298 8 0.6373571688326602 2.4456970787507117 9 0.4459586196336632 1.9251602005030937 >10 1.8029743334545525 12.786662504819784 >50 0.011483912951939824 0.376402328186108 >100 0.011483912951939824 0.9511044195141656 >500 0.0019139854919899705 0.7619852009621211 >1k 0.005741956475969912 6.35201880175532 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT 4247 3.8989772873326847 No Hit CACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTTCTG 1600 1.4688871343848118 RNA PCR Primer, Index 18 (95% over 24bp) TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG 1072 0.9841543800378239 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 830 0.7619852009621211 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 337 0.30938435267980097 No Hit ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT 192 0.1762664561261774 RNA PCR Primer, Index 30 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT 158 0.14505260452050014 No Hit CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT 135 0.12393735196371848 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC 112 0.1028220994069368 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0018361089179810147 0.0 2 0.0 0.0 0.0 0.002754163376971522 0.0 3 0.0 0.0 0.0 0.002754163376971522 0.0 4 0.0 0.0 0.0 0.007344435671924059 0.0 5 0.0 0.0 0.0 0.008262490130914566 0.0 6 0.0 0.0 0.0 0.008262490130914566 0.0 7 0.0 0.0 0.0 0.008262490130914566 0.0 8 0.0 0.0 0.0 0.009180544589905073 0.0 9 0.0 0.0 0.0 0.012852762425867102 0.0 10 0.0 0.0 0.0 0.014688871343848118 0.0 11 0.0 0.0 0.0 0.015606925802838624 0.0 12 0.0 0.0 0.0 0.016524980261829133 0.0 13 0.0 0.0 0.0 0.018361089179810147 0.0 14 0.0 0.0 0.0 0.021115252556781667 0.0 15 0.0 9.180544589905073E-4 0.0 0.022033307015772177 0.0 16 0.0 9.180544589905073E-4 0.0 0.022033307015772177 0.0 17 0.0 9.180544589905073E-4 0.0 0.024787470392743698 0.0 18 0.0 9.180544589905073E-4 0.0 0.029377742687696235 0.0 19 0.0 9.180544589905073E-4 0.0 0.03947634173659181 0.0 20 0.0 9.180544589905073E-4 0.0 0.04314855957255384 0.0 21 0.0 9.180544589905073E-4 0.0 0.04590272294952537 0.0 22 0.0 9.180544589905073E-4 0.0 0.04865688632649689 0.0 23 0.0 9.180544589905073E-4 0.0 0.05141104970346841 0.0 24 0.0 9.180544589905073E-4 0.0 0.05783743091640196 0.0 25 0.0 9.180544589905073E-4 0.0 0.05967353983438298 0.0 26 0.0 9.180544589905073E-4 0.0 0.07344435671924059 0.0 27 0.0 9.180544589905073E-4 0.0 0.13036373317665204 0.0 28 0.0 9.180544589905073E-4 0.0 0.20289003543690212 0.0 29 0.0 9.180544589905073E-4 0.0 0.27082606540219967 0.0 30 0.0 9.180544589905073E-4 0.0 0.3387620953674972 0.0 31 0.0 9.180544589905073E-4 0.0 0.4278133778895764 0.0 32 0.0 9.180544589905073E-4 0.0 0.566439601197143 0.0 33 0.0 9.180544589905073E-4 0.0 0.685786680865909 0.0 34 0.0 9.180544589905073E-4 0.0 0.8482823201072288 0.0 35 0.0 9.180544589905073E-4 0.0 1.0667792813469694 0.0 36 0.0 9.180544589905073E-4 0.0 1.4789857334337073 0.0 37 0.0 9.180544589905073E-4 0.0 2.086737785285423 0.0 38 0.0 9.180544589905073E-4 0.0 2.765180030479408 0.0 39 0.0 9.180544589905073E-4 0.0 3.5427721572443676 0.0 40 0.0 9.180544589905073E-4 0.0 4.39656280410554 0.0 41 0.0 9.180544589905073E-4 0.0 5.5358683877127595 0.0 42 0.0 9.180544589905073E-4 0.0 6.82206268475846 0.0 43 0.0 9.180544589905073E-4 0.0 8.227604061472926 0.0 44 0.0 9.180544589905073E-4 0.0 9.369663808457117 0.0 45 0.0 9.180544589905073E-4 0.0 10.463984723573802 0.0 46 0.0 9.180544589905073E-4 0.0 11.593191708132126 0.0 47 0.0 9.180544589905073E-4 0.0 12.706791766887612 0.0 48 0.0 9.180544589905073E-4 0.0 13.547729651322916 0.0 49 0.0 9.180544589905073E-4 0.0 14.388667535758222 0.0 50 0.0 9.180544589905073E-4 0.0 15.336099737436424 0.0 51 0.0 9.180544589905073E-4 0.0 16.35789435029286 0.0 52 0.0 9.180544589905073E-4 0.0 17.44578888419661 0.0 53 0.0 9.180544589905073E-4 0.0 18.720048473275433 0.0 54 0.0 9.180544589905073E-4 0.0 19.883223472816407 0.0 55 0.0 9.180544589905073E-4 0.0 20.783834897086095 0.0 56 0.0 9.180544589905073E-4 0.0 21.60457558342361 0.0 57 0.0 9.180544589905073E-4 0.0 22.435414868810017 0.0 58 0.0 9.180544589905073E-4 0.0 23.177202871674346 0.0 59 0.0 9.180544589905073E-4 0.0 23.918072820079686 0.0 60 0.0 9.180544589905073E-4 0.0 24.62772891687935 0.0 61 0.0 9.180544589905073E-4 0.0 25.345647503809925 0.0 62 0.0 9.180544589905073E-4 0.0 26.082845234379302 0.0 63 0.0 9.180544589905073E-4 0.0 26.916438683142683 0.0 64 0.0 9.180544589905073E-4 0.0 27.8142959440354 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGGAA 20 7.8964826E-5 70.0 67 CGGAATA 25 2.3814049E-4 55.999996 69 TCGGAAT 25 2.3814049E-4 55.999996 68 TTGCACT 25 2.3814049E-4 55.999996 27 GGCACCC 20 0.0069352346 52.5 12 GTAGACT 20 0.0069352346 52.5 8 GATTCTT 20 0.0069352346 52.5 1 CGGAACT 35 2.0749314E-5 50.0 34 AACTCGT 35 2.0749314E-5 50.0 37 TCTTTAT 65 1.8189894E-10 48.46154 1 CTTCGGA 30 5.85586E-4 46.666664 66 TACCAGG 30 5.85586E-4 46.666664 59 CTTTATA 70 3.783498E-10 45.0 2 GAGACAC 35 0.0012507705 40.0 9 GCTTCGG 35 0.0012507705 40.0 65 CTACCAG 35 0.0012507705 40.0 58 TGAAGTT 45 9.1471404E-5 38.888885 39 TTTCGGT 45 9.1471404E-5 38.888885 27 TTCGGTC 45 9.1471404E-5 38.888885 28 TTTTCGG 45 9.1471404E-5 38.888885 26 >>END_MODULE