FastQCFastQC Report
Wed 25 May 2016
SRR1780243_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780243_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531255
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTAGTC353.2328107E-431.3997337
AGCTAGT353.2328107E-431.3997336
TGTTTCC450.001388575824.47037915
TATTAAG450.001388575824.47037915
AAGTTGT500.002561316122.02541719
CATCGTT500.00258243121.994332
AACAGTA755.4682012E-520.53188330
GGTCCCA755.5040015E-520.514491
TCAATAC755.5040015E-520.514493
GGCCTTA654.9577554E-420.2890571
CTTGTAC1101.2745659E-719.9816473
ACCATTC600.007401732318.33549312
TAACATA600.00744609318.316515
AAGCTTA750.00128368317.61202814
TATAAGA1254.886624E-717.5838492
TTGTACT1154.301244E-617.2015934
TGTACTA1154.301244E-617.2015935
CCTTGTA1206.4489996E-616.484862
TTGTATA953.3653525E-416.195652
AGACCCG953.3653525E-416.195655
Produced by FastQC (version 0.11.5)