FastQCFastQC Report
Wed 25 May 2016
SRR1780243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780243_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531255
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGT147892.783785564371159No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCG39640.746157683221805No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC36350.6842288543166652No Hit
CACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCCGTCTTCTG31200.5872885902250332TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGTGGTGATCTCGTATGCC8570.1613161287893761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG3450.038.89613744
CATCGTT451.0362637E-634.22009732
CGTCTTC3900.033.84405542
CCGTCTT3900.033.84405541
ATGCCGT22300.033.34322444
GTCTTCT4150.031.80525643
CTTTATA1100.030.0066132
TATGCCG26100.028.48865543
GCTATAC407.0178637E-427.506063
ATTAACG407.0294586E-427.49829318
GCCGTCT5000.026.83833740
CATAGAC508.3203064E-526.4058192
TTTTTGC450.001397688524.44292830
ATAACAT450.001397688524.44292836
TCATAAA450.001397688524.44292818
TTTTGCC450.001397688524.44292831
TTCTACG551.5896006E-424.005292
GCGCTAT551.5926489E-423.99851219
CTCGTAT31000.023.9856139
CGTATGC31100.023.90848541
Produced by FastQC (version 0.11.5)