FastQCFastQC Report
Wed 25 May 2016
SRR1780242_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780242_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147684
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGC150.002221055670.030
CTATAAG150.002221055670.028
AGAACCG150.002221055670.05
GCTCCAA252.384195E-455.99999611
CTTGGTA200.006940104552.4999969
ACGATGA200.006940104552.49999614
TACGCTA200.006940104552.49999614
CAAGCTT200.006940104552.49999621
GTTGGCA200.006940104552.49999628
AAGCTTG200.006940104552.49999622
CAGACTG200.006940104552.4999965
TCTTACG400.002412634234.99999611
TTACGCT652.0917016E-532.30769313
CTTACGC552.9809246E-431.81818412
CAGACGG450.00429642731.11111330
GTTTAGG500.00719035327.9999981
ACAGGGG500.00719035327.9999986
GTCACTT500.00719035327.99999822
GTATAGG500.00719035327.9999981
GCCGTAT500.00719035327.99999854
Produced by FastQC (version 0.11.5)