##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780242_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147684 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.553621245361718 34.0 32.0 34.0 12.0 34.0 2 30.59127596760651 34.0 32.0 34.0 12.0 34.0 3 30.371428184502044 34.0 32.0 34.0 11.0 34.0 4 30.24208445058368 34.0 31.0 34.0 11.0 34.0 5 30.229266542076324 34.0 31.0 34.0 11.0 34.0 6 33.309843991224504 38.0 34.0 38.0 21.0 38.0 7 33.46137699412259 38.0 34.0 38.0 21.0 38.0 8 33.50556593808402 38.0 34.0 38.0 21.0 38.0 9 33.78239348880041 38.0 34.0 38.0 21.0 38.0 10-11 33.52833414587904 38.0 34.0 38.0 21.0 38.0 12-13 33.60081999404133 38.0 34.0 38.0 21.0 38.0 14-15 33.50528831830124 38.0 34.0 38.0 16.0 38.0 16-17 33.58789374610655 38.0 34.0 38.0 21.0 38.0 18-19 33.60414127461336 38.0 34.0 38.0 21.0 38.0 20-21 33.60902332006175 38.0 34.0 38.0 21.0 38.0 22-23 33.61863844424582 38.0 34.0 38.0 21.0 38.0 24-25 33.631933723355274 38.0 34.0 38.0 21.0 38.0 26-27 33.6130725061618 38.0 34.0 38.0 21.0 38.0 28-29 33.69726916930744 38.0 34.0 38.0 21.0 38.0 30-31 33.70222231250508 38.0 34.0 38.0 21.0 38.0 32-33 33.757370466672086 38.0 34.5 38.0 21.0 38.0 34-35 33.71218276861407 38.0 34.0 38.0 21.0 38.0 36-37 33.649565965168875 38.0 34.0 38.0 21.0 38.0 38-39 33.65161087186154 38.0 34.0 38.0 21.0 38.0 40-41 33.715388938544464 38.0 34.0 38.0 21.0 38.0 42-43 33.75678814224967 38.0 34.0 38.0 21.0 38.0 44-45 33.83325546436987 38.0 35.0 38.0 21.0 38.0 46-47 33.7870351561442 38.0 35.0 38.0 21.0 38.0 48-49 33.734321253487174 38.0 35.0 38.0 21.0 38.0 50-51 33.70923390482382 38.0 34.0 38.0 21.0 38.0 52-53 33.741749275480075 38.0 34.5 38.0 21.0 38.0 54-55 33.743018878145236 38.0 34.5 38.0 21.0 38.0 56-57 33.704443270767314 38.0 34.5 38.0 21.0 38.0 58-59 33.729872565748494 38.0 35.0 38.0 21.0 38.0 60-61 33.726378619214 38.0 34.5 38.0 20.5 38.0 62-63 33.61648519812573 38.0 34.0 38.0 19.5 38.0 64-65 33.57583082798408 38.0 34.0 38.0 19.0 38.0 66-67 33.47252241271905 38.0 34.0 38.0 19.0 38.0 68-69 33.38380596408548 38.0 34.0 38.0 19.0 38.0 70-71 33.348734460063376 38.0 34.0 38.0 17.5 38.0 72-73 33.347007800438774 38.0 34.0 38.0 14.5 38.0 74-75 33.337985157498444 38.0 34.0 38.0 15.0 38.0 76 31.755085181874815 37.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44578411475703916 1101 2 0.7515877244836311 1101 3 0.2740147842313547 1101 4 0.13981757229644742 1101 5 -0.15920138545996565 1101 6 0.3177708672341666 1101 7 -0.11836983650869115 1101 8 0.03309253083367736 1101 9 0.5600809056933471 1101 10-11 -0.24221985782492794 1101 12-13 0.0661728224386735 1101 14-15 0.10946600696598097 1101 16-17 0.1579332513905598 1101 18-19 0.0469269785641373 1101 20-21 0.013886306648068114 1101 22-23 -0.1198959411656233 1101 24-25 -0.24223153410308385 1101 26-27 -0.43718569463977985 1101 28-29 -0.27117525564307243 1101 30-31 -0.2158608477236612 1101 32-33 -0.08577546209789944 1101 34-35 2.733875166427424E-4 1101 36-37 0.19102482981200808 1101 38-39 -0.15290902839281273 1101 40-41 0.1465203584305499 1101 42-43 -0.2996711184462981 1101 44-45 -0.12658559613802112 1101 46-47 0.05469623025094705 1101 48-49 -0.4696448406259961 1101 50-51 -0.22903204540140365 1101 52-53 0.34641011708436764 1101 54-55 -0.3281328964127326 1101 56-57 0.09366191441913685 1101 58-59 -0.03299611168246486 1101 60-61 0.0016589102208826034 1101 62-63 -0.5253739428427053 1101 64-65 -0.1971358518257489 1101 66-67 -0.5143477124619196 1101 68-69 -0.5303652996109136 1101 70-71 -0.010959701552863521 1101 72-73 -0.17470340794625372 1101 74-75 -0.41455304643427837 1101 76 -1.1178140100872618 1102 1 -0.3787446373307688 1102 2 -0.21353481701686405 1102 3 -0.12631926492159096 1102 4 -0.3548342325915783 1102 5 -0.46369059703579296 1102 6 -0.29004860987664927 1102 7 -0.5585579948070887 1102 8 -0.029313304800503204 1102 9 0.06726296248169916 1102 10-11 -0.026812692811461147 1102 12-13 -0.5202675088835491 1102 14-15 -0.09187359831063446 1102 16-17 -0.14522317223830328 1102 18-19 -0.40770369260196304 1102 20-21 -0.331220554994232 1102 22-23 -0.10548751494617647 1102 24-25 -0.2397807040931923 1102 26-27 0.007992748963864926 1102 28-29 -0.32184846694620717 1102 30-31 -0.053199362227530855 1102 32-33 0.12865000744567823 1102 34-35 0.024573759061105704 1102 36-37 -0.3386066935202976 1102 38-39 -0.052268199405531846 1102 40-41 -0.37477357073739626 1102 42-43 -0.1112401268657024 1102 44-45 -0.5352606481201505 1102 46-47 -0.42084319095776124 1102 48-49 -0.30432459656559274 1102 50-51 -0.428725265567067 1102 52-53 -0.16552702713623546 1102 54-55 -0.16737003722467847 1102 56-57 -0.16700891081862324 1102 58-59 -0.44337890474241703 1102 60-61 0.01949304459044754 1102 62-63 -0.2453828053712499 1102 64-65 -0.2410428634492945 1102 66-67 -0.19322080787043916 1102 68-69 -0.4349431364722136 1102 70-71 -0.45880372105042966 1102 72-73 -0.24502757197121383 1102 74-75 -0.4507496582214401 1102 76 -0.6934829605593613 1103 1 0.7135102189869968 1103 2 0.5580445067758859 1103 3 0.3679152282603333 1103 4 0.3877927333821489 1103 5 0.4706453744639134 1103 6 -0.7115884623822311 1103 7 0.4258761488683973 1103 8 0.31529464748309266 1103 9 0.5389077343916355 1103 10-11 0.47739420420558787 1103 12-13 0.44903597471056145 1103 14-15 0.05602227845798069 1103 16-17 0.5821044529396602 1103 18-19 0.3572464791149983 1103 20-21 0.6398135927295883 1103 22-23 0.07036212981223855 1103 24-25 0.5767047994862082 1103 26-27 0.13193259067388396 1103 28-29 0.30030673280549536 1103 30-31 -0.1798043592250096 1103 32-33 0.20163815256507434 1103 34-35 -0.02481258049991908 1103 36-37 0.3742886097338882 1103 38-39 -0.01626161411044791 1103 40-41 0.6403840782582861 1103 42-43 0.2342820748899257 1103 44-45 0.06417325940233098 1103 46-47 0.14899062480240843 1103 48-49 0.408280575859834 1103 50-51 0.17735128848835302 1103 52-53 0.32982413237498776 1103 54-55 0.16865206669613997 1103 56-57 0.6227508203717917 1103 58-59 -0.19789111385252056 1103 60-61 0.49190362694374556 1103 62-63 0.3186450201993978 1103 64-65 0.45255199246363276 1103 66-67 0.11843030643435526 1103 68-69 0.07283304446146843 1103 70-71 0.1577512789833122 1103 72-73 0.5985446807259933 1103 74-75 0.4422359229510988 1103 76 1.057841183048648 1104 1 0.3414442335327337 1104 2 0.37674598331022935 1104 3 0.025179699717877924 1104 4 0.5041457064738282 1104 5 -0.09881503813894454 1104 6 0.20503837359714794 1104 7 0.27312008060936677 1104 8 -0.14837976571399736 1104 9 0.16807542373811657 1104 10-11 0.46009692087375953 1104 12-13 0.23742845123199174 1104 14-15 0.17462622363373015 1104 16-17 0.5806931548037326 1104 18-19 0.42399086944712394 1104 20-21 -0.35212230785301557 1104 22-23 0.229435129773492 1104 24-25 0.05636208333476134 1104 26-27 -0.0188244076855284 1104 28-29 0.025297367211450705 1104 30-31 0.20646180449848828 1104 32-33 0.3457262020670768 1104 34-35 0.6197082240368985 1104 36-37 0.03792491465179637 1104 38-39 0.2667052768998843 1104 40-41 -0.05073540088550743 1104 42-43 0.1831814561148164 1104 44-45 0.10436134636671568 1104 46-47 0.032615045104428475 1104 48-49 0.20445618397754828 1104 50-51 0.3482798386943955 1104 52-53 0.1957346618862985 1104 54-55 0.4703856704475413 1104 56-57 0.36665349570217387 1104 58-59 0.28837698758008656 1104 60-61 0.35446514920801775 1104 62-63 0.2457968461672806 1104 64-65 0.4416652967743957 1104 66-67 0.48311362017529547 1104 68-69 0.4221309478602109 1104 70-71 0.3785952158915009 1104 72-73 0.21274356718915755 1104 74-75 0.5614405579512933 1104 76 0.8779701052948461 1105 1 -0.005161893549377794 1105 2 0.0411849293092601 1105 3 0.8436502249327873 1105 4 0.676721784835177 1105 5 0.018522920690735134 1105 6 0.7176522041713369 1105 7 0.6179278759311515 1105 8 0.38587781661388476 1105 9 0.4159646158917738 1105 10-11 0.6653745781959017 1105 12-13 -0.32538561911977126 1105 14-15 0.47043904408114656 1105 16-17 0.17381102843584983 1105 18-19 0.28641211271932576 1105 20-21 0.06551792376190946 1105 22-23 0.4436151670155368 1105 24-25 0.17978376024723985 1105 26-27 0.03139496328098801 1105 28-29 0.26103187732547894 1105 30-31 0.8096681917647643 1105 32-33 -0.1379740249563568 1105 34-35 0.20533304866766855 1105 36-37 0.11812285868668937 1105 38-39 0.3758997263042616 1105 40-41 -0.007339487307469028 1105 42-43 0.38044952233423857 1105 44-45 0.2940024452622083 1105 46-47 0.24513066890873603 1105 48-49 0.25314505982888136 1105 50-51 0.18514819060623466 1105 52-53 0.15474290105166943 1105 54-55 0.1073012126254369 1105 56-57 0.11354786603097722 1105 58-59 -0.18642085952345155 1105 60-61 0.0021961901997897826 1105 62-63 0.05356665970196417 1105 64-65 0.13538771597701071 1105 66-67 0.09691868611412957 1105 68-69 0.32864320457525764 1105 70-71 0.17917807944786546 1105 72-73 0.6410590022323959 1105 74-75 0.44370722121685446 1105 76 0.07759768481681917 1106 1 0.3124349573869942 1106 2 0.5496142410480047 1106 3 0.9978695195743406 1106 4 0.596133058376946 1106 5 0.020941709043441392 1106 6 0.6864664014649478 1106 7 -0.25245820245084616 1106 8 0.4041413253811683 1106 9 0.7209638869418598 1106 10-11 -0.1572719076028335 1106 12-13 0.2705673834911977 1106 14-15 0.36506602052943293 1106 16-17 0.7741178500816872 1106 18-19 0.6439057178405321 1106 20-21 0.5093357658050977 1106 22-23 0.7299599470714639 1106 24-25 0.47112847403499813 1106 26-27 0.41217851817091855 1106 28-29 0.6722888520507979 1106 30-31 0.48645511307793754 1106 32-33 0.5247938641372301 1106 34-35 0.12267344177047335 1106 36-37 0.532030560373876 1106 38-39 0.6079841254507272 1106 40-41 0.9290099315206461 1106 42-43 0.32668946613883065 1106 44-45 0.9384206769622452 1106 46-47 0.8840950298847332 1106 48-49 0.7496593538740228 1106 50-51 0.6703372537067978 1106 52-53 0.7308121380399157 1106 54-55 0.5331603795019504 1106 56-57 0.8171402637460474 1106 58-59 0.8242416896806688 1106 60-61 0.79251374728981 1106 62-63 0.8325589858474132 1106 64-65 0.6942106606695901 1106 66-67 0.7021658001351483 1106 68-69 0.5980365857100338 1106 70-71 0.22973406942811891 1106 72-73 0.8092476279630034 1106 74-75 0.5667904142825506 1106 76 0.6637396842601682 1107 1 0.49245702947118986 1107 2 0.15373660652941723 1107 3 0.20222471862685865 1107 4 0.1574638435509641 1107 5 0.4310248831168195 1107 6 0.4909265775032807 1107 7 0.4641914166711203 1107 8 0.039104045092614115 1107 9 0.3308869038189286 1107 10-11 0.1204023138178627 1107 12-13 0.38208860768435216 1107 14-15 0.4764963992541027 1107 16-17 0.3170877566790864 1107 18-19 0.752560262805666 1107 20-21 0.46907776467457296 1107 22-23 0.5231653789575574 1107 24-25 0.3039660827130888 1107 26-27 0.297454150051621 1107 28-29 0.3273553374751472 1107 30-31 0.35119966907912215 1107 32-33 0.4760233061441639 1107 34-35 0.21530217316397682 1107 36-37 0.036623303503269256 1107 38-39 0.12187675283625765 1107 40-41 0.15672587456390374 1107 42-43 0.10923569638835318 1107 44-45 0.2713625896967713 1107 46-47 0.037970430277724176 1107 48-49 0.22432356210529036 1107 50-51 0.3793760413571974 1107 52-53 0.39192825873652737 1107 54-55 0.3231222916981267 1107 56-57 0.7692075028513159 1107 58-59 0.45158348397961134 1107 60-61 0.35192668368453184 1107 62-63 0.47686196557877736 1107 64-65 0.3664503042861824 1107 66-67 0.7011496767468159 1107 68-69 0.43222005318062884 1107 70-71 0.20030686693020527 1107 72-73 0.7247041432654129 1107 74-75 0.6981226799012461 1107 76 0.2842411512462526 1108 1 -0.1084034299816885 1108 2 -0.4418812737097788 1108 3 -0.609258897467523 1108 4 0.02836786714595263 1108 5 0.1609813189747058 1108 6 0.2191377418293854 1108 7 -0.2488467087178492 1108 8 0.27252396554069946 1108 9 0.13550443229797082 1108 10-11 0.469250299314524 1108 12-13 -0.20399454300428488 1108 14-15 -0.022319792536357852 1108 16-17 0.010944806764321413 1108 18-19 0.43350365647939526 1108 20-21 -0.10156176234331582 1108 22-23 0.12548361366275174 1108 24-25 0.11061299669420066 1108 26-27 0.16284839200848467 1108 28-29 0.02067121809316319 1108 30-31 0.39561953541026895 1108 32-33 -0.03407965134697122 1108 34-35 0.045571134389192025 1108 36-37 -0.027583071473053167 1108 38-39 0.18395227303541617 1108 40-41 0.16853351587678134 1108 42-43 0.4779987182786627 1108 44-45 -0.0733183909023083 1108 46-47 0.19989171717872267 1108 48-49 0.20223708322443912 1108 50-51 0.3901723722282071 1108 52-53 0.10481418021284128 1108 54-55 0.18958534335275345 1108 56-57 0.12957901569055963 1108 58-59 0.16948198764603717 1108 60-61 0.2675245406534401 1108 62-63 0.4960870109017179 1108 64-65 0.42693613917537476 1108 66-67 0.4695088509222032 1108 68-69 0.26453192914458157 1108 70-71 0.6461831555899948 1108 72-73 0.041061666873929425 1108 74-75 0.4337647774741029 1108 76 0.5768582467897225 1109 1 0.04872738558542622 1109 2 -0.23367557618437118 1109 3 0.371237665788442 1109 4 0.49430538213673714 1109 5 -0.2636535912176541 1109 6 0.26610086621690954 1109 7 0.34545302470320394 1109 8 -0.005780345637113271 1109 9 0.06561140652410558 1109 10-11 -0.01627974050099823 1109 12-13 0.10346555581495664 1109 14-15 0.18229839650854984 1109 16-17 0.33808651848899984 1109 18-19 0.04468900765775885 1109 20-21 -0.10685222375104075 1109 22-23 0.508027832188958 1109 24-25 -0.05367570351069162 1109 26-27 0.2581315207818804 1109 28-29 -0.33423856309281064 1109 30-31 0.00603735850874898 1109 32-33 -0.21672049617608735 1109 34-35 0.40465777597412966 1109 36-37 0.337513589094506 1109 38-39 0.34949848576404463 1109 40-41 -4.93984928432667E-4 1109 42-43 -0.18767084717709537 1109 44-45 0.1446869955001162 1109 46-47 0.13996970351397664 1109 48-49 0.405011834291777 1109 50-51 0.12986727981645885 1109 52-53 0.290864357361059 1109 54-55 0.16240319934529168 1109 56-57 0.05473817497320255 1109 58-59 -0.0047400335569278695 1109 60-61 0.05230487000522288 1109 62-63 0.34039188401973774 1109 64-65 0.4056989113223253 1109 66-67 0.3731840768975516 1109 68-69 0.030151468216558897 1109 70-71 0.21406827308346976 1109 72-73 0.03640167496038771 1109 74-75 -0.002400186239640334 1109 76 0.2146535223802708 1110 1 0.11372669848909567 1110 2 0.3919158174433015 1110 3 0.5731261991322434 1110 4 -0.18444763617928928 1110 5 0.5000376211474631 1110 6 0.33663621241529995 1110 7 0.3540384569522317 1110 8 0.25669699654340405 1110 9 0.5692857359508068 1110 10-11 0.34989679744961677 1110 12-13 0.36947653626869936 1110 14-15 0.4546699258541054 1110 16-17 0.27073799051109404 1110 18-19 0.33514752994258146 1110 20-21 0.3414552289538264 1110 22-23 0.16716261431398038 1110 24-25 0.2577228974302983 1110 26-27 0.13387832905583252 1110 28-29 0.36058596559183087 1110 30-31 0.2755950647848877 1110 32-33 0.07729129570569881 1110 34-35 0.4748233989087538 1110 36-37 0.5175001089188669 1110 38-39 0.6116159203426577 1110 40-41 0.7082884149354456 1110 42-43 0.17834209640901832 1110 44-45 0.6559161126794848 1110 46-47 0.12081607531816019 1110 48-49 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0.40078246099903225 2116 4 -0.9112646787858054 2116 5 -0.593472669731252 2116 6 0.5408588903221911 2116 7 0.7792492111103542 2116 8 0.830783728524878 2116 9 0.40755565316808884 2116 10-11 0.5091870685819941 2116 12-13 0.7049174111832599 2116 14-15 0.8678714903714635 2116 16-17 0.4060573495146613 2116 18-19 0.8088317404688965 2116 20-21 0.5696583124200671 2116 22-23 0.6428024715327894 2116 24-25 0.2995794327078869 2116 26-27 0.8603251278710502 2116 28-29 0.5705996361318171 2116 30-31 0.9535114466486263 2116 32-33 0.8235318972094632 2116 34-35 1.1061175433607957 2116 36-37 1.092972881760062 2116 38-39 0.7673651418813563 2116 40-41 0.467696404606599 2116 42-43 0.7497516809765017 2116 44-45 0.5497131051606843 2116 46-47 0.736189736744457 2116 48-49 0.860622114640961 2116 50-51 0.8687673773980222 2116 52-53 0.882413003087251 2116 54-55 0.7544067861269497 2116 56-57 0.6283660991233191 2116 58-59 0.689803311116961 2116 60-61 0.6994222579842244 2116 62-63 1.044106576637816 2116 64-65 0.44839084370094184 2116 66-67 0.9033651302509256 2116 68-69 0.8036534594078404 2116 70-71 0.5742134586202994 2116 72-73 0.6158449326020872 2116 74-75 0.7629722060909785 2116 76 1.1666737244004715 2117 1 0.08721104225991638 2117 2 -0.4894775393664439 2117 3 0.010263345796371226 2117 4 -0.10029421465078414 2117 5 -0.13128752716636072 2117 6 0.43156340381150926 2117 7 -0.007536366181554399 2117 8 -0.12021997944086138 2117 9 -0.508503674283638 2117 10-11 0.06239957337677993 2117 12-13 -0.10829701152483295 2117 14-15 -0.06641760309484823 2117 16-17 -0.05217689163097816 2117 18-19 -0.2207041245100001 2117 20-21 -0.6248117042018464 2117 22-23 0.059916651305201185 2117 24-25 -0.23864777608893917 2117 26-27 -0.03473624535487829 2117 28-29 -0.5021468157285582 2117 30-31 -0.30936734123014986 2117 32-33 -0.2560039677694377 2117 34-35 -0.07921041981526855 2117 36-37 -0.2546105474689355 2117 38-39 -0.10002393879886995 2117 40-41 -0.3179544073100331 2117 42-43 -0.0540521804222962 2117 44-45 -0.2784514518013381 2117 46-47 -0.010616937593866282 2117 48-49 -0.16703099877164362 2117 50-51 0.10440540322591829 2117 52-53 -0.2994882883077068 2117 54-55 -0.05693447458140355 2117 56-57 -0.22016350067210766 2117 58-59 -0.00163000943028635 2117 60-61 -0.3907132745563686 2117 62-63 -0.325225989530189 2117 64-65 -0.27290438708795506 2117 66-67 -0.12984993176160486 2117 68-69 -0.5769212752808102 2117 70-71 -0.21837380643532356 2117 72-73 -1.5082888098860927 2117 74-75 -0.6392941281373794 2117 76 -0.6486376168602348 2118 1 0.12277275112067443 2118 2 0.010238969072375426 2118 3 0.5021871327162053 2118 4 -0.10397301211913756 2118 5 0.023083517137436615 2118 6 0.5433052287060249 2118 7 0.35382307474672103 2118 8 0.11006087498952155 2118 9 -0.3010933367309008 2118 10-11 -0.7180856586907325 2118 12-13 0.07044771421355733 2118 14-15 0.1308134095633804 2118 16-17 0.3044159353732425 2118 18-19 0.4741963238022393 2118 20-21 0.34495029474329897 2118 22-23 0.27593062809845037 2118 24-25 0.2808373241220252 2118 26-27 0.3236300521134723 2118 28-29 0.9434689543136301 2118 30-31 0.46511182543650875 2118 32-33 0.21936523054279888 2118 34-35 0.22212133123957756 2118 36-37 0.40014617721460866 2118 38-39 0.03318742723489265 2118 40-41 0.19690252834397626 2118 42-43 0.5543602668350829 2118 44-45 0.4663797718273557 2118 46-47 0.30164901704748104 2118 48-49 0.4359456626207603 2118 50-51 0.48315342537781447 2118 52-53 0.3454022707640263 2118 54-55 0.12222559925825749 2118 56-57 0.2454746849819074 2118 58-59 0.5286211772785805 2118 60-61 0.020405528186252297 2118 62-63 0.012501463001456159 2118 64-65 0.040996588650443755 2118 66-67 0.09753337520042038 2118 68-69 0.09551741142804815 2118 70-71 0.25946440558999484 2118 72-73 0.4885169654303638 2118 74-75 0.13343464295966356 2118 76 0.5792368557136065 2119 1 -0.2926047241268499 2119 2 0.006242475673033709 2119 3 -0.8639690141484806 2119 4 -0.11093464578250334 2119 5 0.04884156169189069 2119 6 0.38584338877203095 2119 7 0.2501463008193241 2119 8 -0.2504743972877037 2119 9 -0.05594069646686961 2119 10-11 0.2749011399891401 2119 12-13 0.05827774721686296 2119 14-15 -0.2154118379613763 2119 16-17 -0.4292886850887925 2119 18-19 -0.09380852355750591 2119 20-21 -0.49587324073524286 2119 22-23 -0.5628430930236661 2119 24-25 -0.06354437967335258 2119 26-27 -0.36518492313405204 2119 28-29 0.05792336850505819 2119 30-31 -0.2526841729133267 2119 32-33 -0.0333162876090185 2119 34-35 -0.5333964080343492 2119 36-37 -0.02122603813192825 2119 38-39 -0.349096504283267 2119 40-41 -0.34379724475834195 2119 42-43 -0.34920821501309973 2119 44-45 -0.46142550870070664 2119 46-47 -0.6254214243716589 2119 48-49 -0.21144759232974053 2119 50-51 -0.17236173551327028 2119 52-53 -0.4287056087739316 2119 54-55 -0.4031714716988333 2119 56-57 -0.5712538628728865 2119 58-59 -0.7608667558897366 2119 60-61 -0.6190720914507182 2119 62-63 -0.6375990939292393 2119 64-65 -0.9271217075541784 2119 66-67 -0.8454972559877021 2119 68-69 -1.0388807940175013 2119 70-71 -0.8402029830238646 2119 72-73 -0.32904955684686144 2119 74-75 -0.4371228034099772 2119 76 -1.0997029132632932 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 27.0 13 297.0 14 1213.0 15 2004.0 16 2580.0 17 3060.0 18 3051.0 19 2785.0 20 2234.0 21 1572.0 22 1249.0 23 1053.0 24 1023.0 25 1014.0 26 1090.0 27 1359.0 28 1536.0 29 1884.0 30 2361.0 31 2930.0 32 3708.0 33 4884.0 34 6759.0 35 10110.0 36 19114.0 37 68784.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.40091005119038 22.11884835188646 11.527315078139813 27.95292651878335 2 19.849814468730536 22.380894341973402 31.409631375098186 26.359659814197883 3 18.98377617074294 22.321307656889033 28.842664066520406 29.852252105847622 4 15.240648953170282 18.72240730207741 30.989816093821943 35.04712765093036 5 15.745104412123181 31.85179166328106 32.41312532163268 19.98997860296308 6 29.177839170119984 33.33604181901899 17.46228433682728 20.023834674033747 7 27.705777199967496 26.17345142330923 20.426721919774653 25.69404945694862 8 24.163755044554588 30.998618672300317 20.577042875328406 24.26058340781669 9 23.25776658270361 17.559789821510794 20.839088865415345 38.34335473037025 10-11 26.74832751008911 23.047520381354786 24.283944096855446 25.92020801170066 12-13 25.383927845941333 20.94268844289158 24.048305842203625 29.625077868963462 14-15 24.282251293301915 22.105983044879608 21.938734053790526 31.67303160802795 16-17 24.944476043444112 23.851602069283064 23.047858942065492 28.15606294520733 18-19 24.124482002112618 23.081037891714743 24.208445058367868 28.58603504780477 20-21 24.284282657566155 23.848893583597413 24.1593537553154 27.707470003521028 22-23 25.618888979171743 23.029238102976628 23.476815362530807 27.87505755532082 24-25 24.742355299152244 23.774748787952657 22.946290728853498 28.5366051840416 26-27 24.087917445356304 24.005985753365294 24.11804934860919 27.78804745266921 28-29 24.599144118523334 23.698234067332955 24.881165190541967 26.82145662360174 30-31 24.40684163484196 23.133176241163568 24.6739660355895 27.786016088404974 32-33 23.86209745131497 23.92066845426722 24.788399555808347 27.428834538609465 34-35 24.31610736437258 23.16635519081282 25.16657186966767 27.350965575146937 36-37 24.431556566723543 22.40764063811923 25.4380975596544 27.722705235502833 38-39 23.316337585655862 22.93274830042523 26.840416023401314 26.910498090517592 40-41 23.0942417594323 22.282034614447063 27.165095744968987 27.45862788115165 42-43 22.250548468351344 22.439803905636357 27.246688876249287 28.062958749763006 44-45 21.36216516345711 22.444543755586253 28.1543701416538 28.038920939302837 46-47 21.66991684948945 22.801386744671053 28.213618266027463 27.315078139812034 48-49 21.275155060805503 23.243547032853932 28.218696676688065 27.2626012296525 50-51 20.980607242490724 24.01038704260448 27.236193494217382 27.772812220687413 52-53 20.372890766772297 23.880718290403834 27.650591804122314 28.09579913870155 54-55 20.115923187345956 23.9044175401533 27.645851954172425 28.333807318328326 56-57 20.425706237642537 24.09536578099185 27.10246201348826 28.376465967877362 58-59 20.669808510062023 23.99345900706915 26.511335012594454 28.82539747027437 60-61 20.598372200102922 23.28823704666721 26.92167059397091 29.19172015925896 62-63 20.489017090544674 23.488326426694837 26.77575092765635 29.246905555104142 64-65 21.35607107066439 23.415535873892907 25.610424961404078 29.61796809403862 66-67 21.170201240486445 23.988719157119252 24.713239078031474 30.12784052436283 68-69 21.283280517862462 24.277511443352022 24.82564123398608 29.613566804799436 70-71 22.174710869153056 23.66031526773381 24.88692072262398 29.278053140489153 72-73 22.210259743777254 23.007231656780693 25.65172936811029 29.13077923133176 74-75 22.259012486119012 22.793599848324803 26.140272473659977 28.80711519189621 76 22.29557704287533 21.893366918555834 26.345440264348202 29.465615774220634 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.0 11 1.0 12 2.0 13 2.0 14 2.0 15 8.0 16 14.5 17 15.0 18 18.0 19 24.5 20 33.5 21 39.0 22 53.0 23 86.5 24 133.0 25 160.0 26 244.5 27 383.0 28 538.5 29 640.0 30 764.5 31 1048.5 32 1489.5 33 1771.0 34 2053.5 35 2593.5 36 3153.0 37 3455.0 38 3923.5 39 4824.5 40 5702.0 41 6462.5 42 6778.0 43 7055.5 44 7579.0 45 7859.5 46 7894.0 47 7864.0 48 7581.0 49 6960.0 50 6592.0 51 6163.0 52 5221.0 53 4364.5 54 4021.0 55 3902.0 56 3648.0 57 3307.0 58 3101.0 59 3165.5 60 3352.0 61 3560.5 62 3647.0 63 3803.5 64 3954.5 65 3962.5 66 3828.0 67 3680.0 68 3514.0 69 3230.5 70 2892.0 71 2671.0 72 2423.0 73 1971.5 74 1412.0 75 1056.0 76 788.5 77 384.5 78 162.0 79 76.0 80 53.0 81 22.5 82 18.0 83 21.0 84 15.0 85 8.5 86 9.0 87 10.0 88 8.5 89 5.5 90 6.5 91 6.5 92 4.0 93 6.5 94 10.0 95 7.0 96 3.0 97 5.0 98 5.0 99 15.5 100 28.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147684.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.84263698166355 #Duplication Level Percentage of deduplicated Percentage of total 1 73.58958111747268 44.037945884456 2 13.892597705311276 16.627393624224695 3 5.410848853787142 9.713983911595028 4 2.3218448030052725 5.557812626960266 5 1.3283848921677341 3.974702743695999 6 0.8112878770734797 2.9129763549199645 7 0.6279843399941162 2.630616722190623 8 0.44581230623005724 2.134286720294683 9 0.3156894249700152 1.700251889168766 >10 1.2559686799882324 10.710029522493974 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004062728528479727 0.0 2 0.0 0.0 0.0 0.011511064164025893 0.0 3 0.0 0.0 0.0 0.011511064164025893 0.0 4 0.0 0.0 0.0 0.04807562092034343 0.0 5 0.0 0.0 0.0 0.05349259229164974 0.0 6 0.0 0.0 0.0 0.05416971371306303 0.0 7 0.0 0.0 0.0 0.056878199398716175 0.0 8 0.0 0.0 0.0 0.056878199398716175 0.0 9 0.0 0.0 0.0 0.06297229219143577 0.0 10 0.0 0.0 0.0 0.06568077787708893 0.0 11 0.0 0.0 0.0 0.06635789929850221 0.0 12 0.0 0.0 0.0 0.06703502071991549 0.0 13 0.0 0.0 0.0 0.06906638498415536 0.0 14 0.0 0.0 0.0 0.0724519920912218 0.0 15 0.0 0.0 0.0 0.07516047777687494 0.0 16 0.0 0.0 0.0 0.07583759919828824 0.0 17 0.0 0.0 0.0 0.07583759919828824 0.0 18 0.0 0.0 0.0 0.07922320630535468 0.0 19 0.0 0.0 0.0 0.08734866336231413 0.0 20 0.0 0.0 0.0 0.09276563473362043 0.0 21 0.0 0.0 0.0 0.09682836326210016 0.0 22 0.0 0.0 0.0 0.1056309417404729 0.0 23 0.0 0.0 0.0 0.10698518458329948 0.0 24 0.0 0.0 0.0 0.11375639879743235 0.0 25 0.0 0.0 0.0 0.1218818558543918 0.0 26 0.0 0.0 0.0 0.1394870128111373 0.0 27 0.0 0.0 0.0 0.23022128328051786 0.0 28 0.0 0.0 0.0 0.3135072181143523 0.0 29 0.0 0.0 0.0 0.4177839170119986 0.0 30 0.0 0.0 0.0 0.5328945586522575 0.0 31 0.0 0.0 0.0 0.6805070285203543 0.0 32 0.0 0.0 0.0 0.8470788981880231 0.0 33 0.0 0.0 0.0 1.051569567454836 0.0 34 0.0 0.0 0.0 1.3677852712548415 0.0 35 0.0 0.0 0.0 1.797080252430866 0.0 36 0.0 0.0 0.0 2.4213862029739173 0.0 37 0.0 0.0 0.0 3.1682511307927737 0.0 38 0.0 0.0 0.0 3.944909401153815 0.0 39 0.0 0.0 0.0 4.76151783537824 0.0 40 0.0 0.0 0.0 5.567969448281466 0.0 41 0.0 0.0 0.0 6.435361989111888 0.0 42 0.0 0.0 0.0 7.300046044256656 0.0 43 0.0 0.0 0.0 8.251401641342326 0.0 44 0.0 0.0 0.0 9.167546924514504 0.0027084856856531515 45 0.0 0.0 0.0 10.06879553641559 0.0027084856856531515 46 0.0 0.0 0.0 11.05739281167899 0.0027084856856531515 47 0.0 0.0 0.0 11.975569459115409 0.0027084856856531515 48 0.0 0.0 0.0 12.790146529075594 0.0027084856856531515 49 0.0 0.0 0.0 13.570867527965115 0.0027084856856531515 50 0.0 0.0 0.0 14.306898513041359 0.0027084856856531515 51 0.0 0.0 0.0 15.114704368787411 0.0027084856856531515 52 0.0 0.0 0.0 15.928604317326183 0.0027084856856531515 53 0.0 0.0 0.0 16.68156333793776 0.0027084856856531515 54 0.0 0.0 0.0 17.364101730722354 0.0027084856856531515 55 0.0 0.0 0.0 18.111643779962623 0.0027084856856531515 56 0.0 0.0 0.0 18.78470247284743 0.0027084856856531515 57 0.0 0.0 0.0 19.456406922889414 0.0027084856856531515 58 0.0 0.0 0.0 20.13555970856694 0.0027084856856531515 59 0.0 0.0 0.0 20.77747081606674 0.0027084856856531515 60 0.0 0.0 0.0 21.423444652095014 0.0027084856856531515 61 0.0 0.0 0.0 22.03894802415969 0.0027084856856531515 62 0.0 0.0 0.0 22.665285338966985 0.0027084856856531515 63 0.0 0.0 0.0 23.314644782102327 0.0027084856856531515 64 0.0 0.0013542428428265758 0.0 24.00733999620812 0.0027084856856531515 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGGC 15 0.0022210556 70.0 30 CTATAAG 15 0.0022210556 70.0 28 AGAACCG 15 0.0022210556 70.0 5 GCTCCAA 25 2.384195E-4 55.999996 11 CTTGGTA 20 0.0069401045 52.499996 9 ACGATGA 20 0.0069401045 52.499996 14 TACGCTA 20 0.0069401045 52.499996 14 CAAGCTT 20 0.0069401045 52.499996 21 GTTGGCA 20 0.0069401045 52.499996 28 AAGCTTG 20 0.0069401045 52.499996 22 CAGACTG 20 0.0069401045 52.499996 5 TCTTACG 40 0.0024126342 34.999996 11 TTACGCT 65 2.0917016E-5 32.307693 13 CTTACGC 55 2.9809246E-4 31.818184 12 CAGACGG 45 0.004296427 31.111113 30 GTTTAGG 50 0.007190353 27.999998 1 ACAGGGG 50 0.007190353 27.999998 6 GTCACTT 50 0.007190353 27.999998 22 GTATAGG 50 0.007190353 27.999998 1 GCCGTAT 50 0.007190353 27.999998 54 >>END_MODULE