FastQCFastQC Report
Wed 25 May 2016
SRR1780242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780242_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147684
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT93986.363587118442079No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC27381.8539584518295822No Hit
CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG24201.6386338398201565Illumina PCR Primer Index 10 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG23771.609517618699385No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC5830.3947617886839468No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT3030.20516779068822621Illumina PCR Primer Index 10 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1690.11443352021884565No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTATC150.002221055670.058
AGAATGC207.905791E-569.999992
TCTTTAT700.060.01
CGCGCTT252.384195E-455.99999618
TCTCGCC252.384195E-455.99999620
TTCCTGG252.384195E-455.99999613
TTAATAC452.6820999E-854.4444473
GGACTAT200.006940104552.4999969
GTCTCGA200.006940104552.4999961
GAATCGA200.006940104552.49999618
GAATCCA200.006940104552.49999637
AAAAACG200.006940104552.49999668
CCTGAAT200.006940104552.49999622
AAAGCAA200.006940104552.49999670
AATCGAA200.006940104552.49999619
CTTAATA506.152368E-848.9999962
CTTTATA800.048.1249962
TAAAGGT305.862679E-446.6666645
AGAATCT305.862679E-446.66666457
AATACAC654.8318543E-737.692315
Produced by FastQC (version 0.11.5)