##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780242_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 147684 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.74497575905311 34.0 34.0 34.0 34.0 34.0 2 33.761544920235096 34.0 34.0 34.0 34.0 34.0 3 33.782298691801415 34.0 34.0 34.0 34.0 34.0 4 33.78724167817773 34.0 34.0 34.0 34.0 34.0 5 33.77774843584952 34.0 34.0 34.0 34.0 34.0 6 37.5632905392595 38.0 38.0 38.0 37.0 38.0 7 37.548617318057474 38.0 38.0 38.0 37.0 38.0 8 37.48894937840254 38.0 38.0 38.0 37.0 38.0 9 37.51099645188375 38.0 38.0 38.0 37.0 38.0 10-11 37.467058042848244 38.0 38.0 38.0 37.0 38.0 12-13 37.44138498415536 38.0 38.0 38.0 37.0 38.0 14-15 37.373053275913435 38.0 38.0 38.0 37.0 38.0 16-17 37.40949256520679 38.0 38.0 38.0 37.0 38.0 18-19 37.37138078600255 38.0 38.0 38.0 36.5 38.0 20-21 37.39045191083665 38.0 38.0 38.0 37.0 38.0 22-23 37.37456325668319 38.0 38.0 38.0 37.0 38.0 24-25 37.398005200292516 38.0 38.0 38.0 37.0 38.0 26-27 37.3039936621435 38.0 38.0 38.0 36.5 38.0 28-29 37.261507678556924 38.0 38.0 38.0 36.0 38.0 30-31 37.264361745348175 38.0 38.0 38.0 36.0 38.0 32-33 37.250863329812304 38.0 38.0 38.0 36.0 38.0 34-35 37.19684258281195 38.0 38.0 38.0 36.0 38.0 36-37 37.15612050052816 38.0 38.0 38.0 36.0 38.0 38-39 36.882448335635544 38.0 38.0 38.0 35.5 38.0 40-41 37.065365916416134 38.0 38.0 38.0 36.0 38.0 42-43 37.17676254705994 38.0 38.0 38.0 36.0 38.0 44-45 37.23457517402021 38.0 38.0 38.0 36.0 38.0 46-47 37.178831153002356 38.0 38.0 38.0 36.0 38.0 48-49 37.19705587605969 38.0 38.0 38.0 36.0 38.0 50-51 37.082354892879394 38.0 38.0 38.0 36.0 38.0 52-53 37.066500094797 38.0 38.0 38.0 36.0 38.0 54-55 37.0540918447496 38.0 38.0 38.0 36.0 38.0 56-57 37.09565017198884 38.0 38.0 38.0 36.0 38.0 58-59 36.99671257549904 38.0 38.0 38.0 36.0 38.0 60-61 37.00677459982124 38.0 38.0 38.0 36.0 38.0 62-63 36.98477831044663 38.0 38.0 38.0 36.0 38.0 64-65 36.908581836894996 38.0 38.0 38.0 35.5 38.0 66-67 36.615151268925544 38.0 37.5 38.0 34.5 38.0 68-69 36.51684678096476 38.0 38.0 38.0 34.0 38.0 70-71 35.87950624305951 38.0 37.0 38.0 31.0 38.0 72-73 34.819946642831994 38.0 37.0 38.0 24.0 38.0 74-75 34.47762113702229 38.0 37.0 38.0 23.0 38.0 76 33.799964789686086 38.0 34.0 38.0 21.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03622266398386387 1101 2 0.06575897573658551 1101 3 0.08191925145607115 1101 4 0.06403392222576798 1101 5 -0.018460617464057805 1101 6 -0.002721432330680784 1101 7 0.12493998133153639 1101 8 -0.12861077188421888 1101 9 -0.06365839711219934 1101 10-11 -0.0380523646634785 1101 12-13 -0.0856727899396148 1101 14-15 -0.0011495850915679284 1101 16-17 0.03889742819027475 1101 18-19 -0.10312332071907093 1101 20-21 -0.031477158902639246 1101 22-23 -0.06338911271128467 1101 24-25 -0.03554223919832822 1101 26-27 -0.17522557361872515 1101 28-29 -0.2438796408535353 1101 30-31 -0.18947180015054244 1101 32-33 -0.1397911136377914 1101 34-35 0.06986498514005746 1101 36-37 0.04013771702436486 1101 38-39 0.028507340877375498 1101 40-41 0.03108280713004774 1101 42-43 -0.07596091763439716 1101 44-45 -0.1601925578824961 1101 46-47 -0.19340284554776588 1101 48-49 0.01908923106057614 1101 50-51 -0.017828574843683498 1101 52-53 0.17203308846983134 1101 54-55 1.946801537044962E-4 1101 56-57 -0.33488647824965767 1101 58-59 -1.377388860603901E-4 1101 60-61 -0.2556798951746586 1101 62-63 -0.10665438927508575 1101 64-65 -0.2122398552693241 1101 66-67 -0.14280847089226967 1101 68-69 -0.7879377956269309 1101 70-71 -0.5890324778514398 1101 72-73 -0.49212915532991985 1101 74-75 -0.4058308618982238 1101 76 -1.2154920449078404 1102 1 -0.015963994502008916 1102 2 0.01652422099001427 1102 3 0.04337406960380008 1102 4 0.08355875434902771 1102 5 0.007983804255950133 1102 6 -0.022941632078435248 1102 7 0.029804146038223678 1102 8 -0.09827365493293172 1102 9 -0.07455248989605678 1102 10-11 -0.024189324816191515 1102 12-13 0.09272650105810243 1102 14-15 0.036611603850715824 1102 16-17 -0.0876322101503888 1102 18-19 0.03223135085606543 1102 20-21 0.11932217299904835 1102 22-23 0.1236647782796112 1102 24-25 0.030118871344669174 1102 26-27 0.0030578227749060716 1102 28-29 -0.13376613231483248 1102 30-31 0.09304856662178906 1102 32-33 0.026994856297783087 1102 34-35 0.03840300879618752 1102 36-37 0.07389713346094595 1102 38-39 0.02399767731259317 1102 40-41 -0.02559647159647227 1102 42-43 -0.2024257913433658 1102 44-45 0.10820571051576877 1102 46-47 0.03461637932184658 1102 48-49 -0.04157136818270146 1102 50-51 -0.15095057095781073 1102 52-53 0.08004004214607363 1102 54-55 -0.15281687522006138 1102 56-57 -0.044485278399641004 1102 58-59 -0.019618258366584485 1102 60-61 0.007203153535165541 1102 62-63 -0.11053344984706115 1102 64-65 0.023742010633803545 1102 66-67 -0.05471834579426371 1102 68-69 0.010351054630426404 1102 70-71 -0.2314294509570729 1102 72-73 -0.04897886729774115 1102 74-75 -0.17635272848308858 1102 76 -0.19817602759182762 1103 1 -0.02577446485130963 1103 2 -0.022265596092616136 1103 3 -0.13482698431329965 1103 4 0.04233993497908273 1103 5 0.027618239411715706 1103 6 -0.08831552974996981 1103 7 0.09729667348257465 1103 8 -0.17631884786992913 1103 9 -0.011857241969138954 1103 10-11 -0.10818557335709755 1103 12-13 -0.03932350940010565 1103 14-15 0.013279687435385767 1103 16-17 -0.21892315739380308 1103 18-19 -0.09045013272454838 1103 20-21 -0.0827741909194657 1103 22-23 0.004046536472436912 1103 24-25 0.07495420523127905 1103 26-27 -0.17503527548498 1103 28-29 -0.19234557081046688 1103 30-31 -0.09728737788900332 1103 32-33 -0.10905963432019661 1103 34-35 -0.1949498915000163 1103 36-37 -0.07390428210761257 1103 38-39 -0.0711888498213824 1103 40-41 0.17014400300358545 1103 42-43 -0.1860300259040173 1103 44-45 -0.03489626034969717 1103 46-47 -0.15066589098562133 1103 48-49 -0.08473676299677635 1103 50-51 -0.13808697967792938 1103 52-53 -0.3137012286269538 1103 54-55 -0.23168860365759514 1103 56-57 -0.31854420869402134 1103 58-59 0.04207839973110339 1103 60-61 0.1410850365517078 1103 62-63 -0.0013126872752877716 1103 64-65 -0.09632492187367347 1103 66-67 -0.6262592103841413 1103 68-69 -0.2973158212003284 1103 70-71 -0.5549624347839668 1103 72-73 -0.24621722664164025 1103 74-75 0.1444746166848887 1103 76 -0.09106377395001175 1104 1 -0.062093592623853056 1104 2 -0.06875123766810276 1104 3 0.08500611434192962 1104 4 0.0862032102242054 1104 5 -0.12146264056658396 1104 6 -0.0038830847486153175 1104 7 -0.06588426979421769 1104 8 -0.3322915132534021 1104 9 -0.17926239110815345 1104 10-11 -0.14682349303259912 1104 12-13 -0.0888836494318852 1104 14-15 -0.012475696166426076 1104 16-17 -0.07121482136921031 1104 18-19 -0.017640712874651854 1104 20-21 0.1677005478127569 1104 22-23 0.02173782065685259 1104 24-25 0.021166629822097605 1104 26-27 0.15295428672193623 1104 28-29 0.09318555525688765 1104 30-31 -0.05368246385869213 1104 32-33 0.10575479773726215 1104 34-35 0.07038707611440742 1104 36-37 -0.16539644730377034 1104 38-39 -0.1719723302053069 1104 40-41 0.007673553067526484 1104 42-43 -0.04211637022201131 1104 44-45 -0.11583114040550413 1104 46-47 -0.034093310861351256 1104 48-49 0.049523871796722574 1104 50-51 -0.17212929924491505 1104 52-53 -0.06814181200657998 1104 54-55 -0.06427376597303436 1104 56-57 -0.33668116597399234 1104 58-59 0.01932646525150261 1104 60-61 -0.145792797347859 1104 62-63 -0.15049045270914263 1104 64-65 -0.125442230783257 1104 66-67 -0.5541215296927575 1104 68-69 -0.04605631027810375 1104 70-71 -0.2989619955699041 1104 72-73 0.21049435181620169 1104 74-75 0.4285879855859491 1104 76 -0.008058774660504753 1105 1 -0.1820844096531573 1105 2 0.07262824859570571 1105 3 0.027459498011545236 1105 4 -0.09012258116931093 1105 5 -0.03856082182822007 1105 6 -0.048828624630765205 1105 7 -0.0912904887060364 1105 8 -0.06390644068242324 1105 9 -0.07168133863767423 1105 10-11 -0.006349549711927693 1105 12-13 -0.07122556204061681 1105 14-15 -0.20957795874836904 1105 16-17 0.004551063894673746 1105 18-19 -0.02294005221092732 1105 20-21 0.0033636348213974543 1105 22-23 -0.056668168680694464 1105 24-25 -0.11653966816530215 1105 26-27 0.029194625471525626 1105 28-29 0.017808670834774887 1105 30-31 0.110488645495181 1105 32-33 0.030951848983342245 1105 34-35 -0.06155435693918321 1105 36-37 0.09105076336888374 1105 38-39 -0.03496750914570157 1105 40-41 0.04399730380089295 1105 42-43 -0.11584159533233418 1105 44-45 -0.00558447560944586 1105 46-47 -0.13177716158705977 1105 48-49 -0.14461305649957268 1105 50-51 -0.32146098304462356 1105 52-53 -0.11892874157829425 1105 54-55 -0.03141913750072689 1105 56-57 0.05325710405636386 1105 58-59 0.06413808681107724 1105 60-61 0.002933065468759821 1105 62-63 0.03186166320035255 1105 64-65 -0.10194913076540502 1105 66-67 0.01800305260960755 1105 68-69 0.07987155238546251 1105 70-71 -0.002243632179350641 1105 72-73 -0.1457220091765805 1105 74-75 -0.1976332219746979 1105 76 -0.6084975165935163 1106 1 0.09251102314428294 1106 2 0.03082628440702706 1106 3 0.10452275761048924 1106 4 0.008471208177418532 1106 5 0.06127218237318033 1106 6 0.09688183646319004 1106 7 0.018873723829251787 1106 8 0.035303642682940506 1106 9 0.17635381000183514 1106 10-11 0.19534426481669698 1106 12-13 0.1969252908307837 1106 14-15 -0.03458690495186545 1106 16-17 0.1326209370573892 1106 18-19 0.1593703214090354 1106 20-21 0.16232773072469797 1106 22-23 0.26202542053614053 1106 24-25 0.1909811069072589 1106 26-27 0.07989971884338587 1106 28-29 0.2195057987721114 1106 30-31 0.1605807582571046 1106 32-33 0.22906718211344668 1106 34-35 -0.00975252528685644 1106 36-37 0.23186400640078375 1106 38-39 0.041277338503768135 1106 40-41 0.04466661235876046 1106 42-43 0.16988357729626102 1106 44-45 0.2800943092973185 1106 46-47 0.09364065925029763 1106 48-49 0.21346039514452997 1106 50-51 0.08305983178884446 1106 52-53 0.0570861555072355 1106 54-55 0.08401008146257993 1106 56-57 0.12221907937809107 1106 58-59 0.08873683301722934 1106 60-61 0.2641091930217456 1106 62-63 0.28828304926548043 1106 64-65 0.36335948012113306 1106 66-67 0.6797272179619611 1106 68-69 0.588234595950162 1106 70-71 0.7024123697291813 1106 72-73 0.8607954657409138 1106 74-75 0.6495623088996467 1106 76 0.8636168357676155 1107 1 0.03019776105174543 1107 2 0.07495699421295399 1107 3 -0.009538775053499648 1107 4 0.059963765578295636 1107 5 0.07611027556044547 1107 6 0.07015981013819328 1107 7 0.09642210745283819 1107 8 0.13384069417549682 1107 9 0.02039569312746181 1107 10-11 -0.1239708276438023 1107 12-13 0.01328958788874246 1107 14-15 0.07091160114487138 1107 16-17 0.045742387354820835 1107 18-19 -0.008542386189681395 1107 20-21 5.050341620247423E-4 1107 22-23 0.07849065700353464 1107 24-25 0.11616481663244116 1107 26-27 -0.017543822380268637 1107 28-29 0.16858186671105813 1107 30-31 -0.14979446717424594 1107 32-33 -0.006824853094215655 1107 34-35 -0.1524539330551562 1107 36-37 0.04620278597603544 1107 38-39 0.19903217686612606 1107 40-41 -0.04573135808618645 1107 42-43 -0.14229509891702463 1107 44-45 0.08543938749171076 1107 46-47 -0.012749478296463224 1107 48-49 -0.0947412683369322 1107 50-51 0.163330214820256 1107 52-53 0.06581404977657002 1107 54-55 0.1170711027002298 1107 56-57 0.005687895829872502 1107 58-59 0.0783198859633174 1107 60-61 -0.1664243054036021 1107 62-63 -0.016813045052295195 1107 64-65 0.2035286545713504 1107 66-67 0.38799886610063794 1107 68-69 0.2613894235456513 1107 70-71 0.18433075685199896 1107 72-73 0.04007395084225607 1107 74-75 0.15905734268070404 1107 76 -0.3055643437430291 1108 1 -0.07370677665424097 1108 2 0.03489692440209069 1108 3 0.04337406960380008 1108 4 -0.026677466123409488 1108 5 -0.07338102514037104 1108 6 -0.0806844142306673 1108 7 0.027179474122213776 1108 8 0.09857673876786066 1108 9 0.15379396679686153 1108 10-11 -0.028126332690206368 1108 12-13 0.06254277402397435 1108 14-15 -0.0946219919498077 1108 16-17 0.013417658616020844 1108 18-19 0.033543686814070384 1108 20-21 0.14556889215916158 1108 22-23 0.04623695675730488 1108 24-25 0.14035509181711348 1108 26-27 -0.06124663916734363 1108 28-29 0.16675880206836524 1108 30-31 0.07730053512572965 1108 32-33 -0.11342509120877509 1108 34-35 0.04102768071219742 1108 36-37 -0.042900766801523105 1108 38-39 -0.2568422177005303 1108 40-41 -0.11483531674083736 1108 42-43 -0.017386421264625085 1108 44-45 -0.041400588696831164 1108 46-47 0.05036441081790599 1108 48-49 0.10541025911388857 1108 50-51 0.11282895660124126 1108 52-53 -0.1693037898749168 1108 54-55 0.12933535575107413 1108 56-57 0.04475356674471698 1108 58-59 0.0722452586937834 1108 60-61 0.14368609316770886 1108 62-63 0.00888912233141781 1108 64-65 0.25471313924272465 1108 66-67 0.2563052762529807 1108 68-69 0.4158628656540486 1108 70-71 0.4050534886754704 1108 72-73 0.19249094897521957 1108 74-75 0.10711183844605188 1108 76 0.3687531062664391 1109 1 -0.08503466876172183 1109 2 -0.05533559548853617 1109 3 0.17762980721884247 1109 4 -0.001972656052345201 1109 5 0.001219963052029982 1109 6 -0.16160380322349255 1109 7 -0.19261452682349045 1109 8 0.04313654085525798 1109 9 -0.1917924040973773 1109 10-11 -0.06575234092741766 1109 12-13 0.023296048010941206 1109 14-15 -0.10247202244534037 1109 16-17 -0.14153049897394254 1109 18-19 0.058848128197546146 1109 20-21 -0.019134410267469093 1109 22-23 0.02964944142387793 1109 24-25 -0.04139487220501081 1109 26-27 0.026374914665836968 1109 28-29 0.008017548119376272 1109 30-31 0.005228277839350426 1109 32-33 -0.15082837924425263 1109 34-35 -0.13246792998658918 1109 36-37 0.207059828855094 1109 38-39 0.06738699907818102 1109 40-41 0.06947078990445732 1109 42-43 -0.061186918787292655 1109 44-45 -0.07369159148597504 1109 46-47 0.01922812661322837 1109 48-49 -0.16121323409829103 1109 50-51 -0.08143102982995032 1109 52-53 0.10147257848372959 1109 54-55 -0.29066354778024106 1109 56-57 0.13396842663657793 1109 58-59 0.18664806017127233 1109 60-61 0.1113676786614306 1109 62-63 0.0767947986127453 1109 64-65 0.1322889840375865 1109 66-67 -0.05525972720775485 1109 68-69 -0.051452088122942996 1109 70-71 0.1358327352644224 1109 72-73 -0.650953881517566 1109 74-75 -0.17811393130923392 1109 76 0.09860378093733146 1110 1 0.04647556897361227 1110 2 -0.20578351685853846 1110 3 -0.0223414001730049 1110 4 0.10654930158325726 1110 5 0.14503904077243845 1110 6 -0.09053186750953301 1110 7 -0.044574714498949675 1110 8 0.053207409933961 1110 9 0.25094629643986366 1110 10-11 -0.1514267096167714 1110 12-13 -0.017115031908176093 1110 14-15 -0.11356699224582201 1110 16-17 -0.07958058502457988 1110 18-19 0.12057228192389857 1110 20-21 0.05977369550610945 1110 22-23 -0.10708927220091624 1110 24-25 0.03260540263352141 1110 26-27 -0.052395771841553085 1110 28-29 -0.12191563994216637 1110 30-31 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0.1320451694121445 2115 54-55 0.09166419255344493 2115 56-57 0.11324936523502771 2115 58-59 0.11733264602617766 2115 60-61 0.1601360718040965 2115 62-63 0.05029383046382918 2115 64-65 -0.12555909416995803 2115 66-67 0.057369387425126206 2115 68-69 0.3100621372567218 2115 70-71 -0.02345980762761002 2115 72-73 -0.02616049866358594 2115 74-75 -0.348248392687843 2115 76 0.10673353313168832 2116 1 0.03983016759136859 2116 2 0.17961178958939428 2116 3 0.03617610571292573 2116 4 -0.0330800748882254 2116 5 0.15967791067446768 2116 6 -0.028767608376853104 2116 7 0.009860616252176158 2116 8 0.11056671729326695 2116 9 0.02703424358044515 2116 10-11 -0.001183677794792004 2116 12-13 -0.051690105956929244 2116 14-15 0.1432587843167994 2116 16-17 -0.24501310572026114 2116 18-19 -0.1736860912818372 2116 20-21 -0.07061857304405805 2116 22-23 0.05280857848639897 2116 24-25 0.003166274510185474 2116 26-27 -0.021618070011221846 2116 28-29 0.07210160012770217 2116 30-31 -0.03893067460941779 2116 32-33 -0.03246788131456668 2116 34-35 0.18025459552966083 2116 36-37 -0.04665881977217623 2116 38-39 0.19795178532181978 2116 40-41 -0.06264013659289702 2116 42-43 -0.06174735342083437 2116 44-45 -0.007955905645850692 2116 46-47 0.07008921794429313 2116 48-49 -0.14617050919915897 2116 50-51 0.13426377724866256 2116 52-53 0.020229336666083952 2116 54-55 5.0380009405159853E-5 2116 56-57 -0.013277531640703444 2116 58-59 -0.008254622002944245 2116 60-61 -0.2485731130678701 2116 62-63 -0.17277993040062967 2116 64-65 -0.14910854213800917 2116 66-67 0.010799032715233636 2116 68-69 -0.18984977253890634 2116 70-71 0.3682186043996367 2116 72-73 0.5364422732415051 2116 74-75 0.7136496575822946 2116 76 0.8018239724081724 2117 1 0.1152297328643499 2117 2 -0.06108633085555937 2117 3 0.10651237984667006 2117 4 0.09796454856146397 2117 5 0.08106264408451835 2117 6 0.09667031516233493 2117 7 0.08979284998187609 2117 8 -0.4625249589629661 2117 9 -0.16650238856378508 2117 10-11 -0.007557543029413694 2117 12-13 -0.14940778911765307 2117 14-15 0.12424906688935522 2117 16-17 0.006035353556050893 2117 18-19 0.1230150978244069 2117 20-21 -0.2153666870959654 2117 22-23 -0.06761400174629983 2117 24-25 -0.24767441082417463 2117 26-27 0.08410370469789541 2117 28-29 -0.7283938608107903 2117 30-31 0.024830353392111704 2117 32-33 -0.12299019303940639 2117 34-35 0.1406688648046952 2117 36-37 -0.05013024286384393 2117 38-39 -0.01947885014678974 2117 40-41 -0.030975387839546897 2117 42-43 0.0562107082081198 2117 44-45 0.07234073161281174 2117 46-47 0.036500162836247796 2117 48-49 -0.04855831127051147 2117 50-51 -0.02719895130267247 2117 52-53 -0.11373051501529119 2117 54-55 0.09384912697270664 2117 56-57 -0.05984127029305597 2117 58-59 0.17658427327898352 2117 60-61 0.00787427217698422 2117 62-63 0.05712992767379177 2117 64-65 -0.1129303040610381 2117 66-67 0.13527799959926057 2117 68-69 -0.10533052947535992 2117 70-71 0.1992792104602472 2117 72-73 0.02217299821273855 2117 74-75 0.06359212018298877 2117 76 -0.5610452259040599 2118 1 -0.15185228190357947 2118 2 -0.1482764679863635 2118 3 -0.18415217711535803 2118 4 -0.08997717084782408 2118 5 -0.03208345296189208 2118 6 -0.05070573968998815 2118 7 -0.05295504031348486 2118 8 -0.0807211614946084 2118 9 0.1615039405501406 2118 10-11 0.07248267321530477 2118 12-13 -0.017007340805413662 2118 14-15 -0.07967366517815577 2118 16-17 0.10647048013832716 2118 18-19 -0.09982245491821118 2118 20-21 -0.04094685587233471 2118 22-23 -0.13417847959440365 2118 24-25 0.07091406491421992 2118 26-27 -0.035625330112232234 2118 28-29 0.08030867083477489 2118 30-31 0.018952848117848475 2118 32-33 0.016813999998568363 2118 34-35 0.09917031522662967 2118 36-37 0.03549080143994843 2118 38-39 0.06742779542283017 2118 40-41 -0.1327158941686548 2118 42-43 -0.014556696855173357 2118 44-45 0.15117446051576167 2118 46-47 -0.08541109771226729 2118 48-49 0.06133422564932545 2118 50-51 -0.0033230283068874655 2118 52-53 0.15312075080749565 2118 54-55 -0.04134008716231108 2118 56-57 -0.20002784729727097 2118 58-59 0.038975491633415515 2118 60-61 0.1513242985482819 2118 62-63 0.02584696353876126 2118 64-65 -0.10878341587537932 2118 66-67 -0.14146580314335466 2118 68-69 -0.17781536597324532 2118 70-71 0.1839146271269101 2118 72-73 0.5543163262718167 2118 74-75 0.31580401364289656 2118 76 -0.0028635275918276193 2119 1 0.10848703327793885 2119 2 -0.09116528811837554 2119 3 0.12711019912226362 2119 4 0.011224355554816157 2119 5 -0.1041234694635449 2119 6 -0.0222919608120975 2119 7 -0.1178271525247041 2119 8 0.02138279890142769 2119 9 0.05816235639172618 2119 10-11 -0.005784137840791459 2119 12-13 -0.059640901036438265 2119 14-15 -0.300873128874521 2119 16-17 -0.3039565000596909 2119 18-19 0.09592837016430167 2119 20-21 0.10117360617385884 2119 22-23 0.14695549317787737 2119 24-25 0.05058351623435442 2119 26-27 0.24922151394717673 2119 28-29 0.29379876984467757 2119 30-31 0.027938512309276575 2119 32-33 -0.21551118564499205 2119 34-35 0.03072714278438582 2119 36-37 0.12282062817261874 2119 38-39 -0.07412542239895004 2119 40-41 0.24537816523728395 2119 42-43 0.06016483779828974 2119 44-45 -0.01844445450073806 2119 46-47 0.04444915393789017 2119 48-49 0.11136774462621446 2119 50-51 0.19917026789772763 2119 52-53 0.01481629536195328 2119 54-55 0.1698423592073226 2119 56-57 0.1553893350459603 2119 58-59 0.1821144149007452 2119 60-61 0.1675810023436668 2119 62-63 0.17247959555196957 2119 64-65 -0.027383869670764227 2119 66-67 0.127498202632232 2119 68-69 0.03272248468681482 2119 70-71 0.17601188372757548 2119 72-73 -0.21085745673148892 2119 74-75 -0.11015772315577976 2119 76 -1.1965258625753208 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 2.0 18 1.0 19 1.0 20 3.0 21 1.0 22 5.0 23 10.0 24 21.0 25 39.0 26 97.0 27 181.0 28 343.0 29 591.0 30 970.0 31 1447.0 32 2457.0 33 4218.0 34 7225.0 35 12310.0 36 20489.0 37 97273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.03989599414967 16.586089217518484 12.40012459034154 33.9738901979903 2 19.80444733349584 18.88356220037377 35.74456271498605 25.567427751144333 3 18.890333414587904 18.863925679152786 31.871428184502044 30.374312721757263 4 15.094390726145013 22.76956203786463 32.31358847268492 29.822458763305438 5 15.52639419300669 29.666043714958967 38.29731047371415 16.510251618320197 6 28.679477803959806 36.96947536632269 16.738441537336477 17.61260529238103 7 27.03881259987541 25.222772947644973 21.91300343977682 25.825411012702798 8 23.469028466184554 36.215839224289695 16.68765743073048 23.627474878795265 9 22.238021722055198 16.427642804907777 17.467024186777174 43.867311286259856 10-11 26.31869396820238 26.027531756994666 23.547574551068497 24.10619972373446 12-13 25.358197231927633 19.076880366187265 25.636832696839196 29.928089705045906 14-15 22.65648276048861 18.932653503426234 20.50899217247637 37.901871563608786 16-17 27.340808753825733 24.91908399014111 19.632797053167575 28.107310202865577 18-19 29.47611115625254 23.549944476043443 20.304840063920263 26.669104303783755 20-21 24.853064651553318 21.63369085344384 20.200901925733323 33.31234256926952 22-23 23.543850383250724 25.663917553695732 20.162306004712764 30.629926058340786 24-25 28.501733430838815 25.170973158906857 20.907478128978088 25.419815281276236 26-27 28.78917147422876 27.32828200752959 20.537431272175727 23.345115246065927 28-29 24.227065897456733 26.969069093469837 21.95837057501151 26.845494434061916 30-31 27.19658189106471 23.31024349286314 24.975285068118417 24.51788954795374 32-33 27.001570921697677 25.1120635952439 24.124820562823327 23.761544920235096 34-35 24.750480756209203 26.023469028466184 25.606700793586306 23.619349421738306 36-37 23.621380786002547 24.575444868773868 28.540667912570083 23.262506432653502 38-39 21.789428780368898 23.46767422334173 28.445870913572225 26.29702608271715 40-41 22.395113891823083 19.91481812518621 28.301982611521897 29.388085371468815 42-43 26.481541670052273 19.659881910024104 27.44305408845914 26.41552233146448 44-45 21.737290430920073 24.34623926762547 29.893895073264538 24.02257522818992 46-47 24.639432843097424 21.319845074618783 27.68207795021803 26.358644132065763 48-49 24.548698572628044 20.344113106362233 24.602868286341106 30.50432003466862 50-51 22.21161398662008 20.83468757617616 27.838154437853795 29.115543999349963 52-53 18.87374393976328 20.9030768397389 34.52608271715284 25.69709650334498 54-55 19.369735380948512 21.656035860350478 30.671230465046996 28.30299829365402 56-57 22.662238292570624 22.67070231033829 25.78816933452507 28.878890062566022 58-59 20.21241298989735 22.96017171799247 30.278500040627286 26.548915251482896 60-61 22.950692018092685 26.20527613011565 25.875856558598088 24.968175293193575 62-63 19.543755586251727 32.91995070556052 22.10666016630102 25.42963354188673 64-65 18.90082879661981 34.67538799057447 21.244346036131198 25.179437176674526 66-67 19.04099293085236 34.48206982476098 20.812004008558816 25.664933235827846 68-69 18.77759269792259 35.03155385823786 20.844505836786652 25.3463476070529 70-71 19.127664472793263 34.07241136480594 21.282941957151756 25.516982205249043 72-73 19.597586739254083 31.99906557243845 21.67905798867853 26.72428969962894 74-75 19.527166111427103 31.133365835161563 22.8240703122884 26.515397741122936 76 19.585059992957937 31.45838411743994 23.003846049673626 25.952709839928495 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.0 15 4.0 16 7.0 17 8.0 18 8.0 19 14.0 20 31.5 21 43.0 22 42.0 23 61.5 24 96.5 25 111.0 26 175.0 27 292.5 28 435.0 29 524.0 30 583.0 31 788.0 32 1178.0 33 1422.0 34 1682.5 35 2094.0 36 2638.5 37 3032.0 38 5659.0 39 9590.0 40 10432.0 41 8917.5 42 7865.0 43 7391.5 44 6953.0 45 7028.5 46 7069.0 47 6951.0 48 6580.0 49 6004.0 50 5681.0 51 5303.0 52 4338.5 53 3560.5 54 3369.0 55 3357.0 56 3196.5 57 2953.5 58 2859.0 59 2894.5 60 3027.0 61 3209.5 62 3295.0 63 3342.0 64 3438.5 65 3526.5 66 3486.5 67 3408.0 68 3340.0 69 3214.5 70 2925.5 71 2694.0 72 2384.5 73 1840.0 74 1336.0 75 1067.0 76 805.5 77 375.5 78 134.0 79 61.0 80 39.0 81 10.5 82 2.5 83 1.0 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 147684.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.21773516426966 #Duplication Level Percentage of deduplicated Percentage of total 1 57.52069917203312 23.70872944936486 2 21.950979103692994 18.0953928658487 3 8.90557234853463 11.0120256764443 4 3.8342752004205547 6.321605590314455 5 1.9401366802470759 3.998401993445465 6 1.3355894335655145 3.3029982936540176 7 1.0037455644631357 2.896048319384632 8 0.7688263898015508 2.5351426017713496 9 0.5864765409383624 2.1755911270008936 >10 2.1208437376790643 13.100945261504293 >50 0.01642791431199895 0.43268058828309097 >100 0.008213957155999475 0.559979415508789 >500 0.0016427914311998948 0.3947617886839468 >1k 0.004928374293599685 5.102109910349124 >5k 0.0016427914311998948 6.363587118442079 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 9398 6.363587118442079 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 2738 1.8539584518295822 No Hit CACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTTCTG 2420 1.6386338398201565 Illumina PCR Primer Index 10 (96% over 26bp) TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 2377 1.609517618699385 No Hit TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 583 0.3947617886839468 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 303 0.20516779068822621 Illumina PCR Primer Index 10 (95% over 23bp) TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 169 0.11443352021884565 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007448335635546166 6.771214214132879E-4 2 0.0 0.0 0.0 0.014896671271092333 6.771214214132879E-4 3 0.0 0.0 0.0 0.014896671271092333 6.771214214132879E-4 4 0.0 0.0 0.0 0.05213834944882316 6.771214214132879E-4 5 0.0 0.0 0.0 0.05823244224154275 6.771214214132879E-4 6 0.0 0.0 0.0 0.05890956366295604 6.771214214132879E-4 7 0.0 0.0 0.0 0.06161804934860919 6.771214214132879E-4 8 0.0 0.0 0.0 0.06161804934860919 6.771214214132879E-4 9 0.0 0.0 0.0 0.06838926356274207 6.771214214132879E-4 10 0.0 0.0 0.0 0.07109774924839522 6.771214214132879E-4 11 0.0 0.0 0.0 0.0717748706698085 6.771214214132879E-4 12 0.0 0.0 0.0 0.0717748706698085 6.771214214132879E-4 13 0.0 0.0 0.0 0.07380623493404838 6.771214214132879E-4 14 0.0 0.0 0.0 0.07380623493404838 6.771214214132879E-4 15 0.0 0.0 0.0 0.07651472061970152 6.771214214132879E-4 16 0.0 0.0 0.0 0.07719184204111482 6.771214214132879E-4 17 0.0 0.0 0.0 0.07719184204111482 6.771214214132879E-4 18 0.0 0.0 0.0 0.08057744914818125 6.771214214132879E-4 19 0.0 0.0 0.0 0.08870290620514071 6.771214214132879E-4 20 0.0 0.0 0.0 0.09547412041927358 6.771214214132879E-4 21 0.0 0.0 0.0 0.10089109179057988 6.771214214132879E-4 22 0.0 0.0 0.0 0.10766230600471277 6.771214214132879E-4 23 0.0 0.0 0.0 0.10901654884753934 6.771214214132879E-4 24 0.0 0.0 0.0 0.11578776306167222 6.771214214132879E-4 25 0.0 0.0 0.0 0.12459034154004496 6.771214214132879E-4 26 0.0 0.0 0.0 0.14219549849679045 6.771214214132879E-4 27 0.0 0.0 0.0 0.23563825465182417 6.771214214132879E-4 28 0.0 0.0 0.0 0.32569540369979144 6.771214214132879E-4 29 0.0 0.0 0.0 0.4306492240188511 6.771214214132879E-4 30 0.0 0.0 0.0 0.5511768370304163 6.771214214132879E-4 31 0.0 0.0 0.0 0.7008206711627529 6.771214214132879E-4 32 0.0 0.0 0.0 0.8714552693589014 6.771214214132879E-4 33 0.0 0.0 0.0 1.0806857885756074 6.771214214132879E-4 34 0.0 0.0 0.0 1.4077354351182254 6.771214214132879E-4 35 0.0 0.0 0.0 1.8417702662441429 6.771214214132879E-4 36 0.0 0.0 0.0 2.4694618238942607 6.771214214132879E-4 37 0.0 0.0 0.0 3.2149725088702907 6.771214214132879E-4 38 0.0 0.0 0.0 3.9929850220741585 6.771214214132879E-4 39 0.0 0.0 0.0 4.821781641884023 6.771214214132879E-4 40 0.0 0.0 0.0 5.633650226158554 6.771214214132879E-4 41 0.0 0.0 0.0 6.5078139812031095 6.771214214132879E-4 42 0.0 0.0 0.0 7.386717586197557 6.771214214132879E-4 43 0.0 0.0 0.0 8.359063947347039 6.771214214132879E-4 44 0.0 0.0 0.0 9.283334687576176 6.771214214132879E-4 45 0.0 0.0 0.0 10.200157092169768 6.771214214132879E-4 46 0.0 0.0 0.0 11.192817095961647 6.771214214132879E-4 47 0.0 0.0 0.0 12.110316621976652 6.771214214132879E-4 48 0.0 0.0 0.0 12.93166490615097 6.771214214132879E-4 49 0.0 0.0 0.0 13.725251212047345 6.771214214132879E-4 50 0.0 0.0 0.0 14.470761897023374 6.771214214132879E-4 51 0.0 0.0 0.0 15.286016088404972 6.771214214132879E-4 52 0.0 0.0 0.0 16.106010129736465 6.771214214132879E-4 53 0.0 0.0 0.0 16.86912597166924 6.771214214132879E-4 54 0.0 0.0 0.0 17.55301860729666 6.771214214132879E-4 55 0.0 0.0 0.0 18.309363235015304 6.771214214132879E-4 56 0.0 0.0 0.0 18.989193142114242 6.771214214132879E-4 57 0.0 0.0 0.0 19.66428319926329 6.771214214132879E-4 58 0.0 0.0 0.0 20.348852956312125 6.771214214132879E-4 59 0.0 0.0 0.0 20.9948267923404 6.771214214132879E-4 60 0.0 0.0 0.0 21.650280328268465 6.771214214132879E-4 61 0.0 0.0 0.0 22.268492186018797 6.771214214132879E-4 62 0.0 0.0 0.0 22.89076677229761 6.771214214132879E-4 63 0.0 0.0 0.0 23.54418894396143 6.771214214132879E-4 64 0.0 0.0 0.0 24.24162400801712 6.771214214132879E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTATC 15 0.0022210556 70.0 58 AGAATGC 20 7.905791E-5 69.99999 2 TCTTTAT 70 0.0 60.0 1 CGCGCTT 25 2.384195E-4 55.999996 18 TCTCGCC 25 2.384195E-4 55.999996 20 TTCCTGG 25 2.384195E-4 55.999996 13 TTAATAC 45 2.6820999E-8 54.444447 3 GGACTAT 20 0.0069401045 52.499996 9 GTCTCGA 20 0.0069401045 52.499996 1 GAATCGA 20 0.0069401045 52.499996 18 GAATCCA 20 0.0069401045 52.499996 37 AAAAACG 20 0.0069401045 52.499996 68 CCTGAAT 20 0.0069401045 52.499996 22 AAAGCAA 20 0.0069401045 52.499996 70 AATCGAA 20 0.0069401045 52.499996 19 CTTAATA 50 6.152368E-8 48.999996 2 CTTTATA 80 0.0 48.124996 2 TAAAGGT 30 5.862679E-4 46.666664 5 AGAATCT 30 5.862679E-4 46.666664 57 AATACAC 65 4.8318543E-7 37.69231 5 >>END_MODULE