FastQCFastQC Report
Wed 25 May 2016
SRR1780241_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780241_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529791
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG301.3114781E-436.6126334
CGTTTAT300.005688248729.38996916
TAAGTGC300.00578366929.2901086
AAGATAC450.001400729624.4337926
GATACCT500.002585074221.99041228
CCGTCTT500.002599290321.9696549
GCTATAT755.527599E-520.5030751
ATTGCGC654.894589E-420.32764813
AACTGCA654.976374E-420.2777657
CTGTTTG550.004511343319.97618132
TTATAGT600.00732476818.3687318
AAGGGAA600.007364879418.3513513
TGCAATG600.00737694818.34614412
AGATACC600.00742538318.32534227
CGTAGAA600.007470011218.3063162
CGATTCT750.001272997417.6339815
AATGAGC750.001272997417.6339815
TAGATCT1154.223988E-617.23431239
AACATCA800.002003509816.47879643
ATTTCTC1351.0610256E-616.3277615
Produced by FastQC (version 0.11.5)