FastQCFastQC Report
Wed 25 May 2016
SRR1780241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780241_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529791
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGT154542.916999345024736No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC47440.8954474500321824No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCG40640.767094948762814No Hit
CACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCCGTCTTCTG38360.7240591101019082RNA PCR Primer, Index 34 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGGTTGGGATCTCGTATGCC8710.16440445383179406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACAGC250.002352247735.19803616
CTTTATA1350.032.5969282
TCTTCTG5150.032.4642144
TTAATAC551.3696626E-732.0042573
GTCTTCT5300.031.96048243
GTATACC353.1810426E-431.4862541
ATGCCGT22700.030.3331144
GACCATA300.00569090229.3871731
GCCTTAC300.00574370329.33169725
CGTCTTC5950.028.09927442
CCGTCTT5950.028.09927441
TAGCAAG407.0291734E-427.4984665
TCTTTAT1600.026.1729491
TATGCCG26300.026.09740343
CTTATAA450.001382329324.4893071
AATACAG450.001397632424.4430815
GCCGTCT7050.024.02702740
TTTATAC1950.022.5671023
TAAGCTT602.8672474E-422.0029284
ATTCGCA500.00257937821.99877239
Produced by FastQC (version 0.11.5)