FastQCFastQC Report
Wed 25 May 2016
SRR1780240_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780240_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175314
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGGG200.00694226352.535
ATGTGAC200.00694226352.519
AAAAGAC200.00694226352.55
GAGAGCG200.00694226352.57
CCTTTGA200.00694226352.545
GGTATGT200.00694226352.51
TTGGGAC200.00694226352.537
TAGATCT1800.040.83333639
GACAGTC350.001252860140.09
AAGGAAA350.001252860140.017
ATATCGC400.002413863835.06
AGTAATT400.002413863835.02
TTAGATC450.00429860331.11111338
AGATCTC2150.030.93023340
ACATATG802.4528908E-630.6258
ATCTCGG1850.030.27027142
CTTCCGA703.4741686E-530.018
ACGCTTC703.4741686E-530.015
GTAGATC1800.029.16666838
TATGACG500.007193972427.99999811
Produced by FastQC (version 0.11.5)