##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780240_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 175314 Sequences flagged as poor quality 0 Sequence length 76 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.28308064387328 34.0 27.0 34.0 12.0 34.0 2 29.315382684782733 34.0 27.0 34.0 12.0 34.0 3 29.10832563286446 34.0 27.0 34.0 11.0 34.0 4 28.9456460978587 34.0 27.0 34.0 11.0 34.0 5 28.94258872651357 34.0 27.0 34.0 11.0 34.0 6 31.557844781363723 37.0 27.0 38.0 11.0 38.0 7 32.01293108365561 37.0 31.0 38.0 12.0 38.0 8 31.957824246780064 37.0 31.0 38.0 11.0 38.0 9 32.320082822820766 37.0 31.0 38.0 12.0 38.0 10-11 31.93327971525377 37.0 30.0 38.0 11.0 38.0 12-13 32.07880431682581 37.0 31.0 38.0 11.0 38.0 14-15 31.917750436359903 37.0 31.0 38.0 11.0 38.0 16-17 31.998060622654208 37.0 31.0 38.0 11.0 38.0 18-19 32.024133839853064 37.0 31.0 38.0 11.0 38.0 20-21 32.01883762848375 37.0 31.0 38.0 11.0 38.0 22-23 32.02460727608748 37.0 31.0 38.0 11.0 38.0 24-25 32.00043921192831 37.0 31.0 38.0 11.0 38.0 26-27 31.962299074802928 37.0 31.0 38.0 11.0 38.0 28-29 32.04363028622928 37.0 31.0 38.0 11.0 38.0 30-31 32.05272824760144 37.0 31.0 38.0 11.0 38.0 32-33 32.111074985454664 37.0 31.0 38.0 11.0 38.0 34-35 32.034412539785755 37.0 31.0 38.0 11.0 38.0 36-37 31.9662092017751 37.0 31.0 38.0 11.0 38.0 38-39 31.951706652064296 37.0 31.0 38.0 11.0 38.0 40-41 32.02544577158698 37.0 31.0 38.0 11.0 38.0 42-43 32.11800825946587 37.0 31.0 38.0 11.0 38.0 44-45 32.21512258005636 37.0 31.0 38.0 11.0 38.0 46-47 32.116975826231794 37.0 31.0 38.0 11.0 38.0 48-49 32.0750824235372 37.0 31.0 38.0 11.0 38.0 50-51 31.99749877362903 37.0 31.0 38.0 11.0 38.0 52-53 32.07446638602735 37.0 31.0 38.0 11.0 38.0 54-55 32.0297380699773 37.0 31.0 38.0 11.0 38.0 56-57 31.969771381635237 37.0 30.5 38.0 11.0 38.0 58-59 32.00983378395337 37.0 31.0 38.0 11.0 38.0 60-61 32.02634415962216 37.0 30.5 38.0 11.0 38.0 62-63 31.855413714820266 37.0 29.0 38.0 11.0 38.0 64-65 31.81419624217119 37.0 29.0 38.0 11.0 38.0 66-67 31.706287005030973 37.0 28.0 38.0 11.0 38.0 68-69 31.611226142806622 37.0 28.0 38.0 11.0 38.0 70-71 31.543581801795636 37.0 27.0 38.0 11.0 38.0 72-73 31.465484787295935 37.0 27.0 38.0 11.0 38.0 74-75 31.4585401051827 37.0 27.0 38.0 11.0 38.0 76 29.60603830840663 35.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09774568759458546 1101 2 0.26958619694002905 1101 3 0.01197422568563411 1101 4 0.2927020751900855 1101 5 0.08017349875765589 1101 6 0.2629524614033585 1101 7 -0.3566269751209603 1101 8 -0.29674282492833015 1101 9 -0.4003405125741608 1101 10-11 -0.33744751846951715 1101 12-13 0.03103702076612791 1101 14-15 -0.11094179896166168 1101 16-17 -0.18425630885780464 1101 18-19 -0.3715687036794293 1101 20-21 -0.2739300186928695 1101 22-23 -0.39207991957030686 1101 24-25 -0.2635994523470302 1101 26-27 -0.48133436788537765 1101 28-29 -0.18077734370852738 1101 30-31 -0.02296876740587095 1101 32-33 -0.25233740884569045 1101 34-35 0.24197307925148692 1101 36-37 -0.16102387115951444 1101 38-39 -0.09815507656672295 1101 40-41 -0.016801302097292137 1101 42-43 -0.43243981509892393 1101 44-45 -0.2296171823422526 1101 46-47 0.38104588243937343 1101 48-49 -0.06817749367563408 1101 50-51 -0.2084415291870343 1101 52-53 0.29219064603926626 1101 54-55 -0.063261900710053 1101 56-57 -0.006036106401616337 1101 58-59 0.5655206238015609 1101 60-61 -0.1290963373084253 1101 62-63 -0.18234508094730728 1101 64-65 -0.053265430701227245 1101 66-67 0.0031462326195637047 1101 68-69 0.17115908707392435 1101 70-71 -0.05846534132678016 1101 72-73 0.1601744928076343 1101 74-75 -0.2768145375624833 1101 76 -1.2444371517754007 1102 1 0.3513559664295798 1102 2 0.4042799643166042 1102 3 0.43857646968200115 1102 4 0.6345246249242074 1102 5 0.14516301657736363 1102 6 0.4321159564231962 1102 7 0.11310066814300512 1102 8 0.05794626370682465 1102 9 0.5787941002149992 1102 10-11 0.3583398147378496 1102 12-13 0.47900851643379383 1102 14-15 0.3012868115421128 1102 16-17 0.3708763604541545 1102 18-19 0.03246086236730861 1102 20-21 0.5876039786618463 1102 22-23 -0.04378811006706229 1102 24-25 0.0010351109554420646 1102 26-27 0.1350673200605641 1102 28-29 0.030168661815984876 1102 30-31 0.19067201322413752 1102 32-33 0.04556120642679673 1102 34-35 -0.043524621866907864 1102 36-37 0.2820821833118359 1102 38-39 0.3306380780746814 1102 40-41 0.21899151963366847 1102 42-43 -0.1346671659415506 1102 44-45 0.5113492476880737 1102 46-47 -0.4403184205631163 1102 48-49 0.3146785180572067 1102 50-51 0.3596409066377291 1102 52-53 0.12156054553629758 1102 54-55 -0.19092181019440346 1102 56-57 -0.17359353345374018 1102 58-59 -0.20147580415931543 1102 60-61 0.3585139955120553 1102 62-63 -0.24352637317969794 1102 64-65 -0.045122621117005934 1102 66-67 0.25927808315177003 1102 68-69 0.16125950006282963 1102 70-71 0.038218150813836615 1102 72-73 0.20260932783701335 1102 74-75 -0.20720701198686342 1102 76 -0.04520374556167184 1103 1 0.10268945898590687 1103 2 0.28876368376569417 1103 3 -0.09160955584675179 1103 4 -0.07258468165966292 1103 5 -0.12024844217061315 1103 6 -0.5810199404003029 1103 7 0.03145907909754442 1103 8 0.3283997838618049 1103 9 -0.24836934218608775 1103 10-11 -0.11974048672434989 1103 12-13 -0.1774479758836982 1103 14-15 -0.06595438475329729 1103 16-17 0.2797610033965938 1103 18-19 -0.11662493228810789 1103 20-21 0.06374043276581176 1103 22-23 0.276999778370687 1103 24-25 -0.4025819046749497 1103 26-27 -0.27454709492762674 1103 28-29 -0.1938249161900103 1103 30-31 -0.1425020971174007 1103 32-33 0.28626656428047426 1103 34-35 0.16564794931903748 1103 36-37 -0.21877364026909163 1103 38-39 -0.38904984486139327 1103 40-41 0.11453460272153393 1103 42-43 -0.13951683290012085 1103 44-45 0.20594628083298971 1103 46-47 0.2363726602956291 1103 48-49 0.2698506115741779 1103 50-51 -0.4551230452769275 1103 52-53 -4.1090047383818273E-4 1103 54-55 0.1299390554479274 1103 56-57 -0.13327031078038942 1103 58-59 -0.053650524855655135 1103 60-61 0.1935390731332589 1103 62-63 -0.11712985485578287 1103 64-65 0.23414787220889366 1103 66-67 0.21582347497302123 1103 68-69 0.14511826926910487 1103 70-71 0.06513347071936693 1103 72-73 0.11950156040872884 1103 74-75 -0.13342705816153355 1103 76 0.6439929744446964 1104 1 0.2602960443707012 1104 2 0.05871527704570312 1104 3 0.41451247050514084 1104 4 0.6727234905336523 1104 5 -0.15500032537322284 1104 6 0.045419965374851046 1104 7 0.4991793037875567 1104 8 -0.06634795758936107 1104 9 0.13769366420774531 1104 10-11 0.04840167889393854 1104 12-13 -0.1321540409609696 1104 14-15 0.2752935767284157 1104 16-17 0.4940966405791656 1104 18-19 0.34261764491672864 1104 20-21 0.24352480283130973 1104 22-23 0.2553655985669039 1104 24-25 0.4166953596982168 1104 26-27 0.46355184115997616 1104 28-29 0.4692112464772791 1104 30-31 0.47839821610255484 1104 32-33 0.2595134385524318 1104 34-35 0.2691723209376846 1104 36-37 -0.14813736370363628 1104 38-39 0.28161620054255465 1104 40-41 0.1495647598492269 1104 42-43 -0.19664064538233106 1104 44-45 0.4214597285822066 1104 46-47 0.5024090041645408 1104 48-49 0.3248906053615528 1104 50-51 0.5555354932025338 1104 52-53 0.504500957878264 1104 54-55 0.5639816637159001 1104 56-57 0.7948055868148103 1104 58-59 0.39918528058557 1104 60-61 0.41637177226090216 1104 62-63 0.44022045965846246 1104 64-65 0.31582450496497216 1104 66-67 0.5064967684419983 1104 68-69 0.34756578551253625 1104 70-71 0.5248614094214403 1104 72-73 0.3806713463319511 1104 74-75 0.5466032283958988 1104 76 0.43390263249134264 1105 1 -4.1469564406426684E-4 1105 2 0.20149544488760185 1105 3 0.6464194022567895 1105 4 0.7547521882739829 1105 5 0.45569217095682646 1105 6 0.7835212644949117 1105 7 0.7137165506034009 1105 8 -0.2954249968514695 1105 9 0.17656544080321623 1105 10-11 0.5077522037180735 1105 12-13 -0.1259002755492986 1105 14-15 0.5574151899792668 1105 16-17 -0.11706043330351434 1105 18-19 0.3663704981441569 1105 20-21 0.20451725927981101 1105 22-23 0.09191915438834286 1105 24-25 0.0035055999208779554 1105 26-27 0.42122649262073963 1105 28-29 -0.07884601323642215 1105 30-31 0.08114410278805906 1105 32-33 0.02587123539414904 1105 34-35 0.06073610170799526 1105 36-37 0.17456371988637898 1105 38-39 0.43448342894500414 1105 40-41 0.3940004360110976 1105 42-43 -0.1343257339278381 1105 44-45 -0.13021020497684077 1105 46-47 -0.18773049638276973 1105 48-49 -0.04338362691873954 1105 50-51 0.3022346590842986 1105 52-53 0.08599507322926314 1105 54-55 -0.1811986093453939 1105 56-57 -0.1836668652212694 1105 58-59 -0.013264125009726513 1105 60-61 0.12164716704575085 1105 62-63 0.10670893219535316 1105 64-65 -0.3126683121145106 1105 66-67 0.31563176322345043 1105 68-69 0.14587548691008578 1105 70-71 0.35164186576812284 1105 72-73 0.045946401699414 1105 74-75 0.1722131190186431 1105 76 0.6377864159769899 1106 1 0.5180030198690879 1106 2 -0.019823384315046155 1106 3 0.575258870515345 1106 4 0.5255012328819468 1106 5 0.00740842599258329 1106 6 1.0418911888399371 1106 7 0.34927007412373 1106 8 0.9077176606480322 1106 9 0.6829534119214813 1106 10-11 -0.14100859027093193 1106 12-13 0.2233893268523346 1106 14-15 0.033642534463872664 1106 16-17 0.4576779661236827 1106 18-19 0.6822645121709527 1106 20-21 0.6501440223339614 1106 22-23 0.47893272397452336 1106 24-25 0.35499066302420346 1106 26-27 0.4698760039258545 1106 28-29 0.32240138801098084 1106 30-31 0.09101720602215835 1106 32-33 0.5272816787133081 1106 34-35 0.5309097544542567 1106 36-37 0.8331027248984171 1106 38-39 0.43218229282643605 1106 40-41 0.4147990833657573 1106 42-43 0.4957520776852391 1106 44-45 0.560236306009088 1106 46-47 0.8871200483847872 1106 48-49 0.9390677839559061 1106 50-51 0.7666349834431259 1106 52-53 0.6196602417492016 1106 54-55 0.4775797828354911 1106 56-57 0.586314415133451 1106 58-59 0.48371010744168785 1106 60-61 0.4754872822966547 1106 62-63 0.6295520753704551 1106 64-65 0.7892523925032933 1106 66-67 0.2657853160741013 1106 68-69 0.8636876175852848 1106 70-71 0.6715989917418455 1106 72-73 0.7539415018107007 1106 74-75 0.6144237624741002 1106 76 0.7484781801957254 1107 1 0.2954009846354708 1107 2 0.443220712413261 1107 3 0.42831938041796036 1107 4 -0.6850119523680789 1107 5 0.03385770928397136 1107 6 0.16232449664166637 1107 7 -0.153844052148294 1107 8 0.41190411430817164 1107 9 0.3850039644983454 1107 10-11 0.3627467440107175 1107 12-13 0.30159044294818926 1107 14-15 0.5699572297259365 1107 16-17 0.4211378632677878 1107 18-19 0.3771392249404002 1107 20-21 0.6106758091815436 1107 22-23 0.7783921834339864 1107 24-25 0.7634007735395727 1107 26-27 0.30186418644034774 1107 28-29 0.8674697762372645 1107 30-31 0.32060249981797995 1107 32-33 0.6611322094059489 1107 34-35 0.38089334127995045 1107 36-37 0.6162496775190576 1107 38-39 -0.17761746644700338 1107 40-41 0.3740615847335249 1107 42-43 0.6561416170221506 1107 44-45 8.074844750041166E-4 1107 46-47 0.6365258914748395 1107 48-49 0.5038035318495488 1107 50-51 0.25936285301310136 1107 52-53 0.22673322566429022 1107 54-55 0.6748158042820407 1107 56-57 0.4174425473142165 1107 58-59 0.4189721711248069 1107 60-61 0.3161306787295288 1107 62-63 0.9349465386596449 1107 64-65 0.6782096088289293 1107 66-67 0.8728254736405674 1107 68-69 0.6435037900330371 1107 70-71 0.16700111451238797 1107 72-73 0.3739874586987568 1107 74-75 0.5320515116807947 1107 76 0.27504782676536976 1108 1 -0.16489141302534804 1108 2 -0.15184001633167554 1108 3 0.21865226390873715 1108 4 0.04369250107321676 1108 5 0.3729085414926985 1108 6 0.20844235539110656 1108 7 0.4120443868980388 1108 8 -0.31473556180518614 1108 9 -0.29989482092675246 1108 10-11 -0.09856229782463899 1108 12-13 0.15853601199902556 1108 14-15 0.13522488604622396 1108 16-17 -0.10148696804125024 1108 18-19 0.3401531700596152 1108 20-21 0.34522371741365987 1108 22-23 0.25356312360492694 1108 24-25 0.05350648654002299 1108 26-27 0.09666625571610155 1108 28-29 0.15192321753280424 1108 30-31 0.1655147805197288 1108 32-33 0.37095227238390294 1108 34-35 0.008704857249362874 1108 36-37 0.045507437303122344 1108 38-39 0.09474870539285618 1108 40-41 0.17190234046901764 1108 42-43 0.13692209385526155 1108 44-45 0.33339407916686525 1108 46-47 0.18629422255895633 1108 48-49 -0.006358411470287706 1108 50-51 0.09982394163208141 1108 52-53 -0.3248997028107432 1108 54-55 -0.157237726982018 1108 56-57 -0.019021690202656316 1108 58-59 -0.3206904469588636 1108 60-61 -0.1310038241944511 1108 62-63 0.315738261556632 1108 64-65 0.30877191202281296 1108 66-67 -0.03515023486145097 1108 68-69 -0.003169624271954774 1108 70-71 0.2514095715524256 1108 72-73 -0.04029504242584636 1108 74-75 0.11846626651660586 1108 76 0.4484666301841749 1109 1 0.31289796298949923 1109 2 0.0838272391685635 1109 3 0.11054331185406596 1109 4 0.2415807297547623 1109 5 0.002929239885006041 1109 6 0.07126197603953699 1109 7 -0.0104927720691812 1109 8 -0.3368971163258223 1109 9 0.42441062310729194 1109 10-11 0.34666279719168713 1109 12-13 0.06517466446231168 1109 14-15 0.17786625071263984 1109 16-17 0.08930180129657828 1109 18-19 0.34514933371966094 1109 20-21 0.28915070205045623 1109 22-23 0.23588696422729782 1109 24-25 -0.16206236237955807 1109 26-27 0.10327502041464243 1109 28-29 -0.22393995895667018 1109 30-31 0.17560524676886757 1109 32-33 -0.2778129614544014 1109 34-35 0.4343490054926704 1109 36-37 -0.07330311887695018 1109 38-39 0.2991587119949308 1109 40-41 0.5297257974574023 1109 42-43 0.4704807006393352 1109 44-45 0.15750235317075578 1109 46-47 0.47408440152867826 1109 48-49 -0.36556027432559546 1109 50-51 0.3025053648249951 1109 52-53 0.181149223906246 1109 54-55 0.3931588435084947 1109 56-57 0.19228926745486064 1109 58-59 0.38471879237521733 1109 60-61 0.2690193722796863 1109 62-63 0.10871865161427152 1109 64-65 0.5110719316505978 1109 66-67 0.6007498775522322 1109 68-69 0.12249950478965843 1109 70-71 0.5545607233465901 1109 72-73 0.054582997650520326 1109 74-75 0.4219207568186931 1109 76 0.06936932989689382 1110 1 0.15326918454701755 1110 2 -0.3147359439334849 1110 3 0.28850071611013206 1110 4 -0.20873154546847772 1110 5 -0.18243154325915256 1110 6 -0.46930310176611556 1110 7 0.10490871211531072 1110 8 0.3227886786602312 1110 9 0.4842034337597383 1110 10-11 0.2749294065494219 1110 12-13 0.382607691953055 1110 14-15 0.2557179550687039 1110 16-17 0.409609778349612 1110 18-19 -0.14692755966817828 1110 20-21 -0.062123836992718395 1110 22-23 -0.11307840480131048 1110 24-25 0.22700537327420278 1110 26-27 -0.2543985291722102 1110 28-29 0.13228844054508926 1110 30-31 -0.06802863684721672 1110 32-33 0.27706984761913134 1110 34-35 -0.421572381851405 1110 36-37 -0.022823741322170576 1110 38-39 0.1946912465118622 1110 40-41 0.21863257102277345 1110 42-43 0.5177846953060907 1110 44-45 0.30913565784962316 1110 46-47 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-0.44659153135523155 2116 22-23 -0.5351177503408522 2116 24-25 -0.4581607461536983 2116 26-27 -0.6138462817831893 2116 28-29 -0.8867930331039702 2116 30-31 -0.48680310027496887 2116 32-33 -0.3092848929525971 2116 34-35 -0.276013827303764 2116 36-37 -0.49108621187330215 2116 38-39 -0.29421093687804856 2116 40-41 -0.8132394493085364 2116 42-43 -0.5141151507250328 2116 44-45 -0.8034146602908798 2116 46-47 -0.6411736554056091 2116 48-49 -1.0969934938272274 2116 50-51 -0.2575443155706054 2116 52-53 -0.3166640091445849 2116 54-55 -0.29311959713541214 2116 56-57 -0.48064505579952765 2116 58-59 -0.392307020231069 2116 60-61 -1.143880939720571 2116 62-63 -0.686582449373347 2116 64-65 -1.0495515158370416 2116 66-67 -0.6265494537441114 2116 68-69 -0.548911685742496 2116 70-71 -0.3148438953632926 2116 72-73 -0.9478203721366079 2116 74-75 -0.4968900475152047 2116 76 -0.8875128796363967 2117 1 -0.5621330602669943 2117 2 -0.4070847715764323 2117 3 0.21263050788698123 2117 4 -0.75176204438133 2117 5 -0.5422041236199675 2117 6 -0.2523191053703542 2117 7 -0.22295949810584403 2117 8 -1.0373462844159107 2117 9 -0.05106032209225475 2117 10-11 -0.2996112488735925 2117 12-13 -0.15168154821854074 2117 14-15 0.20534920617054553 2117 16-17 -0.2981652897195701 2117 18-19 0.16144299134943907 2117 20-21 -1.1466177744278347 2117 22-23 -0.5977383526965525 2117 24-25 -0.6400638320302932 2117 26-27 -0.7204471863973367 2117 28-29 -1.0555359899263976 2117 30-31 -0.6025504875455354 2117 32-33 -0.6082629568313251 2117 34-35 -0.34220811866361345 2117 36-37 -0.40437601258032174 2117 38-39 -0.4959661353219893 2117 40-41 -0.7768156082489313 2117 42-43 -0.6050165230833571 2117 44-45 -0.4913922456194584 2117 46-47 -0.23046956920483552 2117 48-49 -0.6269877820996612 2117 50-51 -0.5067222899141441 2117 52-53 -0.5428484207594586 2117 54-55 -0.46968916443345776 2117 56-57 -0.971236142417105 2117 58-59 -0.483277859546277 2117 60-61 -0.505518198708824 2117 62-63 -0.6978592520408782 2117 64-65 -0.9938698906189956 2117 66-67 -0.857411307122522 2117 68-69 -0.6287523748547059 2117 70-71 -0.7633534432105904 2117 72-73 -1.8784846306154357 2117 74-75 -0.8492882012378651 2117 76 -1.3858379541204116 2118 1 -1.3521069418071399 2118 2 -1.0622199977959959 2118 3 -0.35428049275896 2118 4 -1.2092732079165884 2118 5 -1.5246457783507772 2118 6 -1.4610870442587753 2118 7 -1.6309017566505126 2118 8 -1.4383685553176981 2118 9 -0.8201450526227667 2118 10-11 -2.082884200821244 2118 12-13 -1.5775725247119254 2118 14-15 -1.064564013140263 2118 16-17 -1.4698450156161655 2118 18-19 -1.3292526583823587 2118 20-21 -1.2540039625369133 2118 22-23 -1.401366852710595 2118 24-25 -1.2511711985593479 2118 26-27 -1.8719595294759444 2118 28-29 -1.4547496276942553 2118 30-31 -1.002758311849746 2118 32-33 -1.4007447722047068 2118 34-35 -1.4566462909331115 2118 36-37 -1.4947458836730831 2118 38-39 -1.6924050377849866 2118 40-41 -1.4776188211003394 2118 42-43 -1.6559699872898364 2118 44-45 -1.201247049450167 2118 46-47 -1.7751798789753828 2118 48-49 -1.4254038238891944 2118 50-51 -1.4442008755855014 2118 52-53 -1.5539389366692546 2118 54-55 -1.4536052483495396 2118 56-57 -1.3049036796991018 2118 58-59 -1.231363910041967 2118 60-61 -1.0079783891208223 2118 62-63 -1.0495176338197396 2118 64-65 -0.976286269420914 2118 66-67 -1.3744922181793378 2118 68-69 -1.536217199187 2118 70-71 -1.0055670528940794 2118 72-73 -1.1057208482130996 2118 74-75 -1.5130340938994173 2118 76 -1.0314581973310162 2119 1 -0.21475754421678062 2119 2 -0.5296898773140661 2119 3 -1.3687383240842586 2119 4 -0.01409248502502436 2119 5 -0.4186216819652344 2119 6 -0.5863882490780519 2119 7 -0.869455973517983 2119 8 -0.5540312059201078 2119 9 -0.8586992694902378 2119 10-11 -0.5768601044356991 2119 12-13 -0.6823918018203585 2119 14-15 -0.828419434827012 2119 16-17 -0.4264715216402628 2119 18-19 -0.5220237427197105 2119 20-21 -0.7226786613320932 2119 22-23 -0.8857042021081867 2119 24-25 -0.6704483069930838 2119 26-27 -0.5237667583916092 2119 28-29 -0.3029423987785904 2119 30-31 -0.8340836130545632 2119 32-33 -0.5694194709998897 2119 34-35 -0.6048390620174473 2119 36-37 -0.19715552222730537 2119 38-39 -0.851441182363299 2119 40-41 -0.3944862909798559 2119 42-43 -0.393319384880197 2119 44-45 -0.7711265675224581 2119 46-47 -0.547469035601889 2119 48-49 -0.6290182817205192 2119 50-51 -0.6622731647421318 2119 52-53 -0.8382762860668436 2119 54-55 -0.9451715134097824 2119 56-57 -0.7470723543978934 2119 58-59 -0.45184583775280984 2119 60-61 -0.7152073047834726 2119 62-63 -0.33987907960287345 2119 64-65 -0.4859248236377809 2119 66-67 -1.0407572784203012 2119 68-69 -1.0964581630424206 2119 70-71 -1.156169462532631 2119 72-73 -0.33222687230948367 2119 74-75 -0.7226726481162835 2119 76 -1.2410967515478823 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 32.0 13 458.0 14 1773.0 15 3305.0 16 4535.0 17 5328.0 18 5522.0 19 5061.0 20 3977.0 21 2963.0 22 2327.0 23 1889.0 24 1800.0 25 1763.0 26 1999.0 27 2259.0 28 2581.0 29 2980.0 30 3557.0 31 4255.0 32 5179.0 33 6529.0 34 8815.0 35 12704.0 36 22880.0 37 60840.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.52271923520084 18.34879131158949 11.316266812690372 29.8122226405193 2 20.304140000228163 18.16169843823083 30.125375041354367 31.408786520186638 3 19.20154693863582 19.34985226507866 28.24817185164904 33.20042894463648 4 16.320430769932806 19.891737111696727 26.604264348540337 37.183567769830134 5 16.950728407314873 29.703845671195683 31.217700811116057 22.127725110373387 6 30.70394095132706 28.158778869792883 15.84081043619127 25.296469742688792 7 28.910982579828193 25.7680504694434 17.68426936810523 27.636697582623178 8 24.668879838461276 28.159759060885044 17.357997649930983 29.8133634507227 9 23.462473048358945 16.47158812188416 17.99171771792327 42.07422111183362 10-11 27.55940769134239 21.091869445680324 22.325085275562703 29.02363758741458 12-13 25.51250898388035 18.449467812040112 22.805366371196826 33.23265683288271 14-15 25.23329568659662 19.779652509212042 20.757041650980526 34.23001015321081 16-17 26.745439611211886 21.13037179004529 21.118393282909523 31.005795315833307 18-19 26.03813728509988 19.965889774918146 21.8736096375646 32.12236330241738 20-21 25.653969449102753 20.657791163284163 22.436884675496536 31.251354712116548 22-23 27.458731190891772 20.311840469101156 21.120674903316335 31.10875343669074 24-25 26.401485334884832 21.802023797300844 20.076548364648573 31.71994250316575 26-27 25.44006753596404 22.083233512440536 22.031611850736393 30.44508710085903 28-29 26.33853068930801 21.11417546281376 23.00935181831405 29.537942029564178 30-31 26.098885428431274 20.376581448144474 23.174418471998813 30.350114651425443 32-33 25.085845967806335 21.608086062721746 23.38090511881538 29.925162850656534 34-35 25.24128135802047 21.3702271353115 23.36778580147621 30.020705705191826 36-37 25.630370735853198 20.01956495078816 23.673305250308733 30.676759063049907 38-39 24.35201980446513 20.009240562647594 26.147369862076047 29.491369770811232 40-41 23.97754885519696 19.763681166364353 26.24833156507752 30.010438413361168 42-43 22.704975073297057 19.785071357678223 26.16220039472033 31.347753174304394 44-45 21.751542945800107 19.81130999235657 27.934734248263116 30.502412813580204 46-47 22.116317008339323 20.727950990793662 27.730529221853363 29.425202779013652 48-49 21.698210068790853 21.356252210319767 27.169821006879086 29.775716714010294 50-51 21.28922961086964 22.625973966711157 25.62887162462781 30.45592479779139 52-53 20.569663575071015 22.52016382034521 26.20469557479722 30.705477029786554 54-55 20.530305623053493 22.040738332363645 26.00904662491301 31.41990941966985 56-57 20.95839465188177 22.170790695551982 25.61175947157671 31.259055180989538 58-59 21.212510124690557 22.096638032330564 25.11122899483213 31.579622848146755 60-61 20.989766932475444 21.021709618170824 25.72241806130714 32.26610538804659 62-63 21.429264063337783 21.616642139247293 25.041354369873485 31.912739427541442 64-65 22.0327526609398 21.383916857752375 24.153518829072407 32.429811652235415 66-67 21.582703035695953 22.649930980982695 22.774279293154 32.99308669016735 68-69 21.873894840115447 23.070889946039678 22.770001254891223 32.28521395895365 70-71 22.5272938841165 22.46768655098851 23.023261120047458 31.98175844484753 72-73 22.785972597738915 21.17258176757133 24.56021766658681 31.48122796810295 74-75 22.93541873438516 21.30035251035285 24.942389084727974 30.82183967053401 76 22.80992961201045 20.16838358602279 25.163421061637976 31.858265740328783 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 2.5 15 3.5 16 4.5 17 4.0 18 8.0 19 13.5 20 22.5 21 30.0 22 43.5 23 69.0 24 99.5 25 118.0 26 194.5 27 306.5 28 397.5 29 453.0 30 575.5 31 832.0 32 1173.0 33 1380.0 34 1612.0 35 2108.0 36 2615.5 37 2859.0 38 3350.0 39 4298.5 40 5130.0 41 5807.5 42 6111.0 43 6425.5 44 7032.0 45 7319.5 46 7315.0 47 7349.5 48 7155.5 49 6672.5 50 6418.0 51 6071.0 52 5264.5 53 4497.0 54 4189.0 55 4242.5 56 4205.5 57 4122.5 58 4130.0 59 4405.0 60 5101.0 61 5710.0 62 5898.0 63 6163.0 64 6574.0 65 6725.5 66 6774.5 67 6818.0 68 6633.5 69 6093.0 70 5576.5 71 5416.0 72 4913.5 73 3998.0 74 3056.5 75 2528.0 76 2089.5 77 1275.0 78 640.5 79 382.0 80 258.5 81 94.0 82 37.5 83 22.0 84 21.5 85 16.5 86 9.5 87 7.0 88 9.0 89 15.5 90 14.5 91 8.0 92 7.0 93 8.0 94 7.0 95 6.5 96 8.0 97 8.5 98 8.5 99 20.0 100 32.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 5.704051017032297E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.8520255085161484E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 2.8520255085161484E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 175314.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.995239158991325 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69283868548138 45.06609919452394 2 14.314317907977689 17.748391242058187 3 6.450920582222505 11.997790928716315 4 2.6877552267427296 6.665121123309781 5 1.3013988650500752 4.034026694000965 6 0.7084444876981291 2.635211126742876 7 0.4633236043111507 2.0106700360092957 8 0.33379171473276076 1.6554797747317849 9 0.24112708726403861 1.3453858298380539 >10 0.8060818385195364 6.841824050068812 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0022816204068129187 0.0 2 0.0 0.0 0.0 0.003422430610219378 0.0 3 0.0 0.0 0.0 0.005133645915329067 0.0 4 0.0 0.0 0.0 0.01654174794939366 0.0 5 0.0 0.0 0.0 0.021675393864722725 0.0 6 0.0 0.0 0.0 0.021675393864722725 0.0 7 0.0 0.0 0.0 0.022245798966425957 0.0 8 0.0 0.0 0.0 0.022816204068129186 0.0 9 0.0 0.0 0.0 0.03023147039027117 0.0 10 0.0 0.0 0.0 0.03593552140730347 0.0 11 0.0 0.0 0.0 0.037076331610709924 0.0 12 0.0 0.0 0.0 0.037646736712413156 0.0 13 0.0 0.0 0.0 0.037646736712413156 0.0 14 0.0 0.0 0.0 0.042780382627742225 0.0 15 0.0 0.0 0.0 0.04848443364477452 0.0 16 0.0 0.0 0.0 0.051336459153290666 0.0 17 0.0 0.0 0.0 0.05418848466180681 0.0 18 0.0 0.0 0.0 0.06331496628905849 0.0 19 0.0 0.0 0.0 0.06901901730609078 0.0 20 0.0 0.0 0.0 0.07757509383163923 0.0 21 0.0 0.0 0.0 0.08727198056059414 0.0 22 0.0 0.0 0.0 0.09582805708614257 0.0 23 0.0 0.0 0.0 0.10153210810317488 0.0 24 0.0 0.0 0.0 0.1135106152389427 0.0 25 0.0 0.0 0.0 0.11978507135767823 0.0 26 0.0 0.0 0.0 0.1386084397138848 0.0 27 0.0 0.0 0.0 0.24356297842727906 0.0 28 0.0 0.0 0.0 0.3827418232428671 0.0 29 0.0 0.0 0.0 0.5048085150073582 0.0 30 0.0 0.0 0.0 0.6479801955348689 0.0 31 0.0 0.0 0.0 0.8259465872662765 0.0 32 0.0 0.0011408102034064593 0.0 1.0039129789976842 0.0 33 0.0 0.0011408102034064593 0.0 1.266869730882873 0.0 34 0.0 0.0011408102034064593 0.0 1.6638716816683208 0.0 35 0.0 0.0011408102034064593 0.0 2.1778066783029306 0.0 36 0.0 0.0011408102034064593 0.0 2.883968194211529 0.0 37 0.0 0.0011408102034064593 0.0 3.821143776309935 0.0 38 0.0 0.0011408102034064593 0.0 4.717820596187412 0.0 39 0.0 0.0011408102034064593 0.0 5.588258781386541 0.0 40 0.0 0.0011408102034064593 0.0 6.448429674755011 0.0 41 0.0 0.0011408102034064593 0.0 7.374767559921056 0.0 42 0.0 0.0011408102034064593 0.0 8.350160283833578 0.0 43 0.0 0.0011408102034064593 0.0 9.298743967966049 0.0 44 0.0 0.0011408102034064593 0.0 10.259306159234288 0.0 45 0.0 0.0011408102034064593 0.0 11.197622551536101 0.0 46 0.0 0.0011408102034064593 0.0 12.113122739769784 0.0 47 0.0 0.0011408102034064593 0.0 12.965878366816113 0.0 48 0.0 0.0011408102034064593 0.0 13.738206874522286 0.0 49 0.0 0.0011408102034064593 0.0 14.50540173631313 5.704051017032297E-4 50 5.704051017032297E-4 0.0011408102034064593 0.0 15.19787352978085 5.704051017032297E-4 51 5.704051017032297E-4 0.0011408102034064593 0.0 15.886922892638351 5.704051017032297E-4 52 5.704051017032297E-4 0.0011408102034064593 0.0 16.66210342585304 5.704051017032297E-4 53 5.704051017032297E-4 0.0011408102034064593 0.0 17.40191884276213 5.704051017032297E-4 54 5.704051017032297E-4 0.0011408102034064593 0.0 18.183373832095555 5.704051017032297E-4 55 5.704051017032297E-4 0.0011408102034064593 0.0 18.92946370512338 5.704051017032297E-4 56 5.704051017032297E-4 0.0011408102034064593 0.0 19.58542957208209 5.704051017032297E-4 57 5.704051017032297E-4 0.0011408102034064593 0.0 20.235691388023774 5.704051017032297E-4 58 5.704051017032297E-4 0.0011408102034064593 0.0 20.89736130599952 5.704051017032297E-4 59 5.704051017032297E-4 0.0011408102034064593 0.0 21.535074209703733 5.704051017032297E-4 60 5.704051017032297E-4 0.0011408102034064593 0.0 22.134569971593827 5.704051017032297E-4 61 5.704051017032297E-4 0.0011408102034064593 0.0 22.791106243654244 5.704051017032297E-4 62 5.704051017032297E-4 0.0011408102034064593 0.0 23.370637826984723 5.704051017032297E-4 63 5.704051017032297E-4 0.0011408102034064593 0.0 23.955303056230534 5.704051017032297E-4 64 5.704051017032297E-4 0.0011408102034064593 0.0 24.568488540561507 5.704051017032297E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTGGG 20 0.006942263 52.5 35 ATGTGAC 20 0.006942263 52.5 19 AAAAGAC 20 0.006942263 52.5 5 GAGAGCG 20 0.006942263 52.5 7 CCTTTGA 20 0.006942263 52.5 45 GGTATGT 20 0.006942263 52.5 1 TTGGGAC 20 0.006942263 52.5 37 TAGATCT 180 0.0 40.833336 39 GACAGTC 35 0.0012528601 40.0 9 AAGGAAA 35 0.0012528601 40.0 17 ATATCGC 40 0.0024138638 35.0 6 AGTAATT 40 0.0024138638 35.0 2 TTAGATC 45 0.004298603 31.111113 38 AGATCTC 215 0.0 30.930233 40 ACATATG 80 2.4528908E-6 30.625 8 ATCTCGG 185 0.0 30.270271 42 CTTCCGA 70 3.4741686E-5 30.0 18 ACGCTTC 70 3.4741686E-5 30.0 15 GTAGATC 180 0.0 29.166668 38 TATGACG 50 0.0071939724 27.999998 11 >>END_MODULE