FastQCFastQC Report
Wed 25 May 2016
SRR1780240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780240_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175314
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT169039.641557434089691No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG42732.4373409995779003No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC42042.3979830475603774No Hit
CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG37732.1521384487262853RNA PCR Primer, Index 34 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC11500.6559658669587141No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT5360.30573713451293105RNA PCR Primer, Index 24 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3350.19108570907058192No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3020.17226234071437535No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG2860.1631358590871237No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG2280.13005236318833635No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGC2030.11579223564575562No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT2030.11579223564575562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA1150.063.913042
TCTTTAT1250.058.8000031
TTTATAC1350.057.0370373
TCGGTAG200.00694226352.59
GTCCTCA459.170022E-538.888891
TTGAAAA34100.036.7448758
TCGTATG34650.036.66666840
CTCGTAT34400.036.62790739
CGTATGC34600.036.61849641
CTTGAAA34250.036.58394257
GTCTTCT34200.036.53508849
CTGCTTG34300.036.53061354
TATGCCG34650.036.4646543
CTTCTGC34500.036.42028851
GTATGCC34700.036.41210642
TCTCGTA33550.036.40834438
GCTTGAA34550.036.36758456
TCTTCTG34400.036.32267450
CCGTCTT34400.036.32267447
TGCTTGA34650.036.26262755
Produced by FastQC (version 0.11.5)