##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780240_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 175314 Sequences flagged as poor quality 0 Sequence length 76 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.70942423309034 34.0 34.0 34.0 34.0 34.0 2 33.725315719223794 34.0 34.0 34.0 34.0 34.0 3 33.741635009183526 34.0 34.0 34.0 34.0 34.0 4 33.75129196755536 34.0 34.0 34.0 34.0 34.0 5 33.7466830943336 34.0 34.0 34.0 34.0 34.0 6 37.49447847861551 38.0 38.0 38.0 37.0 38.0 7 37.48345254799959 38.0 38.0 38.0 37.0 38.0 8 37.40754874111594 38.0 38.0 38.0 37.0 38.0 9 37.44343863011511 38.0 38.0 38.0 37.0 38.0 10-11 37.391337828125536 38.0 38.0 38.0 37.0 38.0 12-13 37.3633965342186 38.0 38.0 38.0 37.0 38.0 14-15 37.2872417490902 38.0 38.0 38.0 36.0 38.0 16-17 37.308517859383734 38.0 38.0 38.0 36.0 38.0 18-19 37.25363918454887 38.0 38.0 38.0 36.0 38.0 20-21 37.295181788105914 38.0 38.0 38.0 36.0 38.0 22-23 37.27392564199094 38.0 38.0 38.0 36.0 38.0 24-25 37.27622722657631 38.0 38.0 38.0 36.0 38.0 26-27 37.1662074905598 38.0 38.0 38.0 36.0 38.0 28-29 37.12686094664431 38.0 38.0 38.0 36.0 38.0 30-31 37.10105867186876 38.0 38.0 38.0 36.0 38.0 32-33 37.04944841826665 38.0 38.0 38.0 36.0 38.0 34-35 37.11869844963893 38.0 38.0 38.0 36.0 38.0 36-37 37.108317076787934 38.0 38.0 38.0 36.0 38.0 38-39 36.351363838598175 38.0 37.0 38.0 34.0 38.0 40-41 36.71950329123744 38.0 37.0 38.0 34.5 38.0 42-43 36.97713816352373 38.0 38.0 38.0 35.5 38.0 44-45 37.05240882074449 38.0 38.0 38.0 36.0 38.0 46-47 37.00093261234129 38.0 38.0 38.0 35.5 38.0 48-49 37.035650318856455 38.0 38.0 38.0 36.0 38.0 50-51 36.84003274125284 38.0 37.0 38.0 34.5 38.0 52-53 36.836818508504734 38.0 37.5 38.0 35.0 38.0 54-55 36.818260378520826 38.0 37.5 38.0 35.0 38.0 56-57 36.86113202596484 38.0 37.5 38.0 35.0 38.0 58-59 36.70340645926737 38.0 37.0 38.0 34.5 38.0 60-61 36.70407098121086 38.0 37.0 38.0 34.0 38.0 62-63 36.69981005510113 38.0 37.0 38.0 34.0 38.0 64-65 36.6138300420959 38.0 37.0 38.0 34.5 38.0 66-67 36.21757532199368 38.0 37.0 38.0 33.5 38.0 68-69 36.082292344022726 38.0 37.0 38.0 33.5 38.0 70-71 35.12653011168532 38.0 36.5 38.0 27.0 38.0 72-73 33.51243197919162 38.0 34.5 38.0 20.0 38.0 74-75 33.04490228960608 38.0 34.0 38.0 11.0 38.0 76 32.32862178719326 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.11658415982114434 1101 2 -0.2160938443070748 1101 3 -0.3022358034302002 1101 4 -0.06609342414817121 1101 5 -0.051407055745343655 1101 6 -0.251500816446935 1101 7 -0.07310910296866524 1101 8 -0.3444436072818675 1101 9 -0.06538076498188161 1101 10-11 0.017146668404969034 1101 12-13 -0.07864761205301107 1101 14-15 0.1272831300722288 1101 16-17 -0.03876813565144488 1101 18-19 -0.026042728640291557 1101 20-21 -0.11618004510752655 1101 22-23 -0.17940830638095662 1101 24-25 -0.19877171509473612 1101 26-27 -0.05761454287532786 1101 28-29 -0.18297804967689757 1101 30-31 -0.09841767503299792 1101 32-33 0.031395478786436115 1101 34-35 0.007115517611666178 1101 36-37 0.02719416659102336 1101 38-39 0.17330825902630664 1101 40-41 0.012036128756975017 1101 42-43 0.17217273364306607 1101 44-45 -0.09805036324171823 1101 46-47 0.08437005904969652 1101 48-49 0.15100990992044672 1101 50-51 -0.08841264465567633 1101 52-53 -0.12465365674366069 1101 54-55 -0.18941820229129291 1101 56-57 -0.17459821199828696 1101 58-59 -0.15253067982560253 1101 60-61 -0.12459020742377902 1101 62-63 -0.15574575482668962 1101 64-65 -0.08820209505319099 1101 66-67 0.13866597291635685 1101 68-69 -0.4277364747577792 1101 70-71 -0.41257363778117906 1101 72-73 -0.1082971747606365 1101 74-75 -0.2792442444837633 1101 76 -1.3433617310254498 1102 1 -0.020930890550580727 1102 2 0.08464575674024388 1102 3 -0.027067323751232664 1102 4 -0.12006090689531135 1102 5 -0.13442772318580154 1102 6 0.07214662239201886 1102 7 0.040831746942707525 1102 8 0.061225767656274854 1102 9 -0.07423888214836438 1102 10-11 0.07912199704156109 1102 12-13 0.09801086681617477 1102 14-15 0.09618974419303328 1102 16-17 0.10436311204292537 1102 18-19 0.17421190286383847 1102 20-21 0.16612351523474445 1102 22-23 0.048700582327988684 1102 24-25 0.09090086298176914 1102 26-27 0.16398234756114505 1102 28-29 0.07706999739843212 1102 30-31 0.15927075677883806 1102 32-33 0.14925927342567746 1102 34-35 0.22412364242609328 1102 36-37 0.15837212971893422 1102 38-39 -0.030136014464126504 1102 40-41 0.3550800999287276 1102 42-43 0.23652117391263516 1102 44-45 0.23929364122107444 1102 46-47 0.2546290093920547 1102 48-49 0.19914021286895434 1102 50-51 0.1050825551355743 1102 52-53 0.17555283770933272 1102 54-55 0.09214980591450228 1102 56-57 0.19905777454133045 1102 58-59 0.0603902944323238 1102 60-61 0.2547095109519759 1102 62-63 0.1791239152320685 1102 64-65 0.19609667857670132 1102 66-67 0.1420220701262096 1102 68-69 -0.010716653089801298 1102 70-71 -0.09848387792658286 1102 72-73 -0.35448894917332296 1102 74-75 -0.3962600172408912 1102 76 -0.1287649575575145 1103 1 -0.04408456727482957 1103 2 0.10239912032594845 1103 3 0.06179237425582329 1103 4 -0.02312064205440123 1103 5 0.055074174221516614 1103 6 0.0018629400949592423 1103 7 0.10751964334161102 1103 8 0.11260171967708033 1103 9 0.08003492832320092 1103 10-11 -0.0466243607325012 1103 12-13 -0.12580358532405 1103 14-15 0.13751474485646042 1103 16-17 0.026668926335837284 1103 18-19 0.059816817546852974 1103 20-21 0.08141688359713584 1103 22-23 0.18268672992769552 1103 24-25 0.037302423367087556 1103 26-27 0.06697573980698479 1103 28-29 0.03620939712775595 1103 30-31 -0.12280604177753673 1103 32-33 0.06501040788949553 1103 34-35 -0.19875272092077978 1103 36-37 -0.09735989798586075 1103 38-39 -0.08596357596400139 1103 40-41 0.30088457409479474 1103 42-43 0.12404339878857229 1103 44-45 0.03760455064973911 1103 46-47 -0.023089059763798048 1103 48-49 -0.10317780852000169 1103 50-51 -0.11576636878236712 1103 52-53 -0.023754542276343216 1103 54-55 -0.20917970606703307 1103 56-57 -0.2645730404150157 1103 58-59 -0.09518383713893996 1103 60-61 -0.08615374303523282 1103 62-63 0.0783092242573602 1103 64-65 0.21605262688230198 1103 66-67 -0.033216100932364157 1103 68-69 0.19536433833869182 1103 70-71 -0.1941105530851388 1103 72-73 -0.36481190647501904 1103 74-75 -0.29382655918288236 1103 76 0.3729599581869891 1104 1 0.027943078916052855 1104 2 -0.0025942787576909154 1104 3 0.0946545833624981 1104 4 0.013274998752208944 1104 5 -0.04709765669147714 1104 6 -0.025485562403034123 1104 7 -0.07867796997411602 1104 8 -0.1441092119581313 1104 9 -0.07935778737144972 1104 10-11 -0.0038737200553171647 1104 12-13 -0.04237317969126764 1104 14-15 -0.0016675180929865974 1104 16-17 0.011946294252794587 1104 18-19 0.041068920780006124 1104 20-21 0.009894013363386023 1104 22-23 -0.0403336423122127 1104 24-25 -0.010075330250472803 1104 26-27 0.06975847905783894 1104 28-29 -0.1673641577892795 1104 30-31 -0.05480366027045136 1104 32-33 -0.1866592356610255 1104 34-35 -0.10762771768503399 1104 36-37 -0.05253191010048397 1104 38-39 0.003297639122330054 1104 40-41 -0.22926608211394495 1104 42-43 -0.09219485546319106 1104 44-45 -0.10344150044912936 1104 46-47 -0.14914714782778304 1104 48-49 -0.16855923794197025 1104 50-51 -0.35866067646272626 1104 52-53 -0.10177478508651916 1104 54-55 -0.2262126588769462 1104 56-57 -0.4716088406790533 1104 58-59 -0.009203665773974024 1104 60-61 -0.17417243268862137 1104 62-63 -0.04757413366762364 1104 64-65 -0.5070454031971678 1104 66-67 -0.5987722604933339 1104 68-69 -0.3608793519618487 1104 70-71 -0.5712687938762144 1104 72-73 0.24790844764472553 1104 74-75 0.0736503376098554 1104 76 -0.5137760828163493 1105 1 -0.22087956701221145 1105 2 -0.04403714208279297 1105 3 8.735584916905736E-4 1105 4 -0.0749353382044049 1105 5 0.11860167041354686 1105 6 0.018600513847083278 1105 7 -0.08921687993517224 1105 8 -0.053408399902025394 1105 9 0.03182686417417102 1105 10-11 0.030725640142897248 1105 12-13 0.02362653180394858 1105 14-15 -0.03516589313223051 1105 16-17 -0.08226536643163485 1105 18-19 -0.10821109008135466 1105 20-21 -0.19331305670085186 1105 22-23 -0.009769106338218592 1105 24-25 0.048735096651753906 1105 26-27 -0.006553157018828415 1105 28-29 -0.09005336177064294 1105 30-31 0.008179479170692616 1105 32-33 0.07053853436672597 1105 34-35 -0.05131412995837081 1105 36-37 -0.11508229236931555 1105 38-39 -0.5887035658094035 1105 40-41 -0.32441962605577146 1105 42-43 -0.20879533573879883 1105 44-45 -0.2534427732643323 1105 46-47 -0.2680599090731093 1105 48-49 -0.19747547821553724 1105 50-51 -0.21586353775132494 1105 52-53 -0.04653672621456906 1105 54-55 -0.17745535079626507 1105 56-57 -0.02998438905027001 1105 58-59 0.013163940230320748 1105 60-61 0.030882340792700802 1105 62-63 0.013100967521189943 1105 64-65 -0.1692529739020472 1105 66-67 -0.3456581059396271 1105 68-69 -0.056990477215350666 1105 70-71 -0.3667167824039055 1105 72-73 -0.29019751846444564 1105 74-75 -0.312074191236384 1105 76 -0.3577331804582471 1106 1 0.012956407487664023 1106 2 -0.005208974623919005 1106 3 0.02942748746055912 1106 4 -0.002804317940608314 1106 5 0.003967566152887514 1106 6 -0.04271279435419473 1106 7 -0.07797646620512921 1106 8 -0.03191962737591325 1106 9 0.04530155814678949 1106 10-11 -0.022461444164520117 1106 12-13 -0.056630712353708645 1106 14-15 -0.043000970469378785 1106 16-17 -0.0641689904514422 1106 18-19 -0.028911394599077767 1106 20-21 0.10729037149593523 1106 22-23 -0.031020648336642864 1106 24-25 -0.012964250317310189 1106 26-27 -0.1475737658817593 1106 28-29 0.032698339819226874 1106 30-31 0.02296972425139643 1106 32-33 0.20694177450440066 1106 34-35 -0.052194039551963556 1106 36-37 -0.044422101377172396 1106 38-39 0.009606921505231014 1106 40-41 0.173343937060487 1106 42-43 0.1649743004789741 1106 44-45 0.10686295218661002 1106 46-47 0.008997310930169533 1106 48-49 0.14314227007855607 1106 50-51 0.11598315094183675 1106 52-53 0.07256378189008217 1106 54-55 0.19077818756175446 1106 56-57 0.11835370327122519 1106 58-59 0.10298804457723776 1106 60-61 0.14367467350204777 1106 62-63 0.21733919873037166 1106 64-65 0.19967500290974272 1106 66-67 0.14254291026968247 1106 68-69 0.4420002147214035 1106 70-71 0.20664999324502986 1106 72-73 0.24985777781491691 1106 74-75 0.3849500752899573 1106 76 0.48019523630834016 1107 1 0.01559564591637752 1107 2 -0.025648847695372012 1107 3 0.015907408677321655 1107 4 0.006886020170782103 1107 5 0.06813213558061193 1107 6 -0.10232982028700377 1107 7 0.00940727381019002 1107 8 -3.4300753739557877E-5 1107 9 0.15470958966008652 1107 10-11 -0.001023650095142159 1107 12-13 0.17893991900639605 1107 14-15 0.2766642007747322 1107 16-17 0.1902198923584919 1107 18-19 -0.14189093284177545 1107 20-21 0.07959587538039159 1107 22-23 -0.06567214193550086 1107 24-25 0.16506835718898571 1107 26-27 0.10435970605171008 1107 28-29 0.05269396297274653 1107 30-31 -0.029086299383578762 1107 32-33 0.24689581761634116 1107 34-35 -0.12070919438291128 1107 36-37 0.14894478777918607 1107 38-39 0.19237308848192214 1107 40-41 0.13235914660202042 1107 42-43 -0.07725351438042338 1107 44-45 0.06266117956378991 1107 46-47 0.1253120061922388 1107 48-49 -0.004086091886648546 1107 50-51 -0.15412079916211496 1107 52-53 0.028224138603071935 1107 54-55 0.08116006741731496 1107 56-57 -0.050080479899804686 1107 58-59 0.05829606514841856 1107 60-61 -0.1653875871391648 1107 62-63 -0.2644356125181275 1107 64-65 0.0342849164670298 1107 66-67 0.21175836053777175 1107 68-69 0.151644595944731 1107 70-71 -0.07718850111977815 1107 72-73 0.20320497116211556 1107 74-75 0.5160957148566467 1107 76 0.08922467060093453 1108 1 -0.1105132159819604 1108 2 -0.19260791202285787 1108 3 -0.07972012168705334 1108 4 0.046283231146944104 1108 5 0.007605819791137947 1108 6 0.04235304071164592 1108 7 0.19217830394963897 1108 8 0.15536380990752718 1108 9 0.02699478984002468 1108 10-11 -0.15979108149415566 1108 12-13 -0.19956210528510354 1108 14-15 -0.03572446319287792 1108 16-17 0.03495898367653183 1108 18-19 0.19842170773402756 1108 20-21 0.1619508011563653 1108 22-23 0.03166703935104209 1108 24-25 0.1491979118448512 1108 26-27 0.1854708171628232 1108 28-29 0.15056620768709905 1108 30-31 0.1312510825327564 1108 32-33 -0.13450544194280667 1108 34-35 0.14115315379522997 1108 36-37 0.2296307976757177 1108 38-39 -0.24891121201290645 1108 40-41 -0.14826613454957993 1108 42-43 0.15865066915533532 1108 44-45 0.04634089166455624 1108 46-47 0.09455402148687853 1108 48-49 0.07436195129824341 1108 50-51 0.038742448701135856 1108 52-53 -0.006958047631741238 1108 54-55 0.0633238101073772 1108 56-57 -0.09107688115935986 1108 58-59 0.10477829636749192 1108 60-61 0.3578985127258818 1108 62-63 -0.2511292697380938 1108 64-65 0.09647564971038491 1108 66-67 0.13627702900380712 1108 68-69 0.006478154199342612 1108 70-71 0.3641632757583153 1108 72-73 -0.2501710972139577 1108 74-75 -0.2762592109193278 1108 76 0.16066416677727346 1109 1 0.08272233529117301 1109 2 0.04370616823694462 1109 3 0.02217485181543566 1109 4 0.03588942638987902 1109 5 0.14278327281827785 1109 6 -0.09512621628744711 1109 7 0.1826409843914405 1109 8 0.05934378956600028 1109 9 0.059893635369348885 1109 10-11 -0.08983586853268122 1109 12-13 -0.013557608737805538 1109 14-15 0.012211942781107155 1109 16-17 0.04531933198050808 1109 18-19 0.018990289001976635 1109 20-21 0.06716866957882672 1109 22-23 -0.028904753736405553 1109 24-25 -0.04632159995858842 1109 26-27 0.028952607930619934 1109 28-29 -0.13988487451764087 1109 30-31 -0.07252548189099173 1109 32-33 0.05870111373764786 1109 34-35 -0.0988500580242686 1109 36-37 -0.05879282347232362 1109 38-39 -0.33011894536051756 1109 40-41 0.06237123318590676 1109 42-43 -0.041050899283177955 1109 44-45 0.015703702363481398 1109 46-47 0.09894127770085959 1109 48-49 0.011316611113024067 1109 50-51 0.1262132300654386 1109 52-53 0.05025248713620556 1109 54-55 -0.2758666329452808 1109 56-57 0.12043704564683821 1109 58-59 0.011318809901744942 1109 60-61 0.084659660416051 1109 62-63 -0.3183901230114756 1109 64-65 0.03818558672304562 1109 66-67 0.17620842097068845 1109 68-69 0.25938440475694335 1109 70-71 0.34282028912305407 1109 72-73 -0.279133233430791 1109 74-75 0.0017170751467290302 1109 76 -0.15077972452048272 1110 1 0.02460493384207041 1110 2 0.033815990871630675 1110 3 0.11097153959284611 1110 4 0.049371646000061276 1110 5 0.0055049794550043885 1110 6 -0.17037051024720284 1110 7 0.01674915793226006 1110 8 0.001248317556736822 1110 9 0.006381416285471175 1110 10-11 -0.08586663992265642 1110 12-13 0.13051223214217345 1110 14-15 -0.010191172953696537 1110 16-17 0.0064450139860880995 1110 18-19 0.12153454261156327 1110 20-21 0.07883293862674634 1110 22-23 -0.19744127063962225 1110 24-25 0.008684013978012217 1110 26-27 0.13633449596767377 1110 28-29 0.145789938204949 1110 30-31 -0.019793950865221177 1110 32-33 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-0.05066086422212379 2115 56-57 -0.032330933620308144 2115 58-59 -0.1841842736295618 2115 60-61 -0.052114749873581445 2115 62-63 0.023937043259252277 2115 64-65 -0.240851199685423 2115 66-67 -0.07724345434426283 2115 68-69 -0.18530640224038564 2115 70-71 -0.5136071339086499 2115 72-73 -0.24415135067696525 2115 74-75 -0.39453949385478637 2115 76 -0.15532463369428484 2116 1 0.04573855285259043 2116 2 -0.14643425492204898 2116 3 0.032438139477832806 2116 4 0.03578342925641209 2116 5 -0.06689749146713808 2116 6 0.013613016938506917 2116 7 0.011870385231127045 2116 8 0.10055824020129478 2116 9 -0.10071506469102331 2116 10-11 -0.03639718326581942 2116 12-13 -0.050343222622508677 2116 14-15 0.09112220007763483 2116 16-17 -0.24799086632629752 2116 18-19 -0.11229176937018792 2116 20-21 -0.03521764849552511 2116 22-23 -0.039433034232473574 2116 24-25 0.06497308589817408 2116 26-27 0.15438178110689194 2116 28-29 0.11472254435999218 2116 30-31 0.16228351588306822 2116 32-33 -0.1829078305769869 2116 34-35 -4.2295347242315984E-5 2116 36-37 -0.2472257710682655 2116 38-39 -0.17406120918276713 2116 40-41 -0.2917398425928255 2116 42-43 -0.08139432999561791 2116 44-45 -0.05387419861676079 2116 46-47 0.20913912365476506 2116 48-49 0.04706529651814151 2116 50-51 -0.09902146141376988 2116 52-53 0.03035728604120891 2116 54-55 0.06645110959229328 2116 56-57 0.06641307186039569 2116 58-59 0.18966820410508234 2116 60-61 0.12545242680297974 2116 62-63 0.16192897444597776 2116 64-65 0.02760430766024058 2116 66-67 0.1089025455034971 2116 68-69 -0.03288195221374224 2116 70-71 -0.03693057152759138 2116 72-73 -0.5435499957253072 2116 74-75 -0.04321879658753147 2116 76 -0.1540610270869962 2117 1 0.005648400179659063 2117 2 0.0954651260501791 2117 3 0.019347477380549094 2117 4 0.06454274940646343 2117 5 0.0711256333109489 2117 6 0.004474252832856962 2117 7 0.05192966370100294 2117 8 -0.48741008286636855 2117 9 0.019030531875763756 2117 10-11 0.027466105763032544 2117 12-13 -0.06844322416622362 2117 14-15 0.05401912655071328 2117 16-17 -0.15725158353404112 2117 18-19 -0.04856741425510336 2117 20-21 0.16049392469949453 2117 22-23 0.22154660423061046 2117 24-25 -0.2727135100665663 2117 26-27 0.02016390185590211 2117 28-29 -0.6744143672934726 2117 30-31 0.050248751530425295 2117 32-33 -0.1944316268689903 2117 34-35 -0.09078157813950583 2117 36-37 0.001095444140368329 2117 38-39 -0.07185215995385619 2117 40-41 -0.3885536248370727 2117 42-43 -0.2565591210544511 2117 44-45 -0.19919135386769682 2117 46-47 0.052498854431711095 2117 48-49 -0.2537266267903391 2117 50-51 -0.10568257072388576 2117 52-53 0.05539626472256742 2117 54-55 0.03671153349509382 2117 56-57 -0.3421452572277346 2117 58-59 -0.11530717371797294 2117 60-61 -0.5299220000946292 2117 62-63 0.08803156942273915 2117 64-65 -0.08514828191353985 2117 66-67 0.11286988059666214 2117 68-69 -0.22419395147276333 2117 70-71 -0.01977223317719279 2117 72-73 -0.35516332720619204 2117 74-75 -0.01664382630394101 2117 76 -0.4246116684985637 2118 1 0.012450433487714463 2118 2 -0.356530268113886 2118 3 -0.27814458878621906 2118 4 -0.23742596461044485 2118 5 0.0720636511164372 2118 6 0.057289710467593125 2118 7 0.12583086290340617 2118 8 -0.008785093398003596 2118 9 0.09507737659730964 2118 10-11 0.08961680646795145 2118 12-13 0.1020597668187051 2118 14-15 0.09133763133910122 2118 16-17 -0.07095956230225653 2118 18-19 -0.11040076163061485 2118 20-21 -0.052236718151633 2118 22-23 -0.0010304171415924657 2118 24-25 -0.04255371002724928 2118 26-27 -0.1503390891290195 2118 28-29 -0.2830194168623805 2118 30-31 -0.08062719982745392 2118 32-33 -0.047853706797098994 2118 34-35 0.08758503516686744 2118 36-37 0.02025740667582454 2118 38-39 -0.3266503737882047 2118 40-41 -0.3643561355793423 2118 42-43 -0.032954211907373576 2118 44-45 0.01179244145345848 2118 46-47 0.12765823320555114 2118 48-49 -0.002082287194198784 2118 50-51 -0.013638871451270518 2118 52-53 -0.11098955828975932 2118 54-55 -0.020678867268799195 2118 56-57 -0.1553055908700003 2118 58-59 -0.30909772052056894 2118 60-61 -0.18006101770352956 2118 62-63 0.1166804306017255 2118 64-65 -0.18746524206182613 2118 66-67 -0.26673244478880065 2118 68-69 -0.1907904072258475 2118 70-71 -0.40553345990227996 2118 72-73 -0.8772769565487337 2118 74-75 -0.49158976390048537 2118 76 -0.733831765670466 2119 1 0.0895588672226566 2119 2 0.019373346343940057 2119 3 0.12185541121377952 2119 4 0.14329692695581997 2119 5 -0.03637008382332141 2119 6 0.03801260203385226 2119 7 -0.011518534686956627 2119 8 0.01772093069838121 2119 9 -0.05432021376413587 2119 10-11 -0.04050367545976741 2119 12-13 -0.16179565486805103 2119 14-15 -0.5508310433596932 2119 16-17 -0.1287908876034649 2119 18-19 -0.053774255606519716 2119 20-21 -0.07392346513958614 2119 22-23 0.05800572743671495 2119 24-25 -0.022071782316402278 2119 26-27 0.06652838075050482 2119 28-29 0.023004679523161542 2119 30-31 -0.19869948898408296 2119 32-33 -0.26351635739951007 2119 34-35 0.0863802158897613 2119 36-37 -0.0773329547699646 2119 38-39 -0.02544555451110142 2119 40-41 -0.0944766175070555 2119 42-43 -0.08114698299171152 2119 44-45 -0.061701534450158135 2119 46-47 -0.01330562221613718 2119 48-49 -0.02376903418214482 2119 50-51 0.15503582734390875 2119 52-53 0.04322730918011786 2119 54-55 0.02751390381554586 2119 56-57 0.16638115611795 2119 58-59 0.1451191469493054 2119 60-61 0.19825223530851588 2119 62-63 0.301017779999313 2119 64-65 -0.05373030230279596 2119 66-67 -0.008901119487596532 2119 68-69 0.4055951349428284 2119 70-71 0.3294062991338649 2119 72-73 0.1492290675476582 2119 74-75 0.136121079473007 2119 76 -1.049494416272875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 2.0 20 1.0 21 4.0 22 9.0 23 21.0 24 40.0 25 85.0 26 164.0 27 328.0 28 603.0 29 1027.0 30 1585.0 31 2524.0 32 3964.0 33 6646.0 34 11180.0 35 18775.0 36 27314.0 37 101042.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.90993303444106 14.261838757885851 12.254583204992185 38.573645002680905 2 18.971673682649417 16.69518692175183 37.36552699727346 26.96761239832529 3 18.902654665343327 15.365002224579897 33.40349316084283 32.32884994923395 4 15.684429081533704 24.34660095599895 28.670842032011134 31.29812793045621 5 16.56513455856349 27.362332728703926 38.853143502515486 17.219389210217095 6 30.27710279840743 36.254377859155575 14.827680618775455 18.640838723661542 7 27.84147301413464 22.402660369394344 19.767388799525424 29.988477816945597 8 24.035159770468987 36.94970167813181 13.859703161185074 25.15543539021413 9 21.800312581995733 15.010210251320489 14.183693258952509 49.00578390773127 10-11 27.187788767582738 25.66224032307745 21.60409322700982 25.545877682329994 12-13 25.447197599735333 16.118222161379013 24.99486635408467 33.43971388480099 14-15 22.4211985351997 15.156233957356516 18.398701757988523 44.02386574945527 16-17 30.542911575801135 23.036950842488334 16.08656467823448 30.333572903476046 18-19 33.329910902723114 20.907058192728474 16.322712390339618 29.44031851420879 20-21 26.04868977948139 18.152286754052728 16.437934220883673 39.361089245582214 22-23 25.38359743089542 23.497552962113694 16.141893973099695 34.97695563389119 24-25 31.66033517003776 23.74596438390545 17.09846332865601 27.495237117400777 26-27 31.767000924056266 26.503587848089715 17.970612729160248 23.75879849869377 28-29 31.635807750664526 21.160318057884712 17.279281745895936 29.924592445554833 30-31 31.950386164253853 17.728760966038077 23.566571979419784 26.754280890288285 32-33 26.25745804670477 23.603933513581346 25.27065722075818 24.867951218955703 34-35 28.346281529141997 25.25183385240198 21.92865372987896 24.473230888577067 36-37 27.500370763316106 23.041514083301962 18.762049807773483 30.696065345608453 38-39 22.32109243985078 20.90991021823699 26.151362697787967 30.61763464412426 40-41 22.839305474748166 16.14674241646417 27.720547132573554 33.293404976214106 42-43 29.289446364808285 15.983891759927902 26.44112848945321 28.285533385810602 44-45 22.150256111890666 23.155024698540906 30.000456324081366 24.69426286548707 46-47 26.821873894840113 18.473139623760794 26.245764742119853 28.459221739279233 48-49 26.6416258827019 16.862600819101726 21.648870027493526 34.84690327070285 50-51 23.323579406094208 17.660597556384545 27.121051370683457 31.89477166683779 52-53 18.66279932007712 17.66116796148625 36.68731533134832 26.98871738708831 54-55 19.601971320031485 18.505082309456174 30.64586969665857 31.24707667385377 56-57 25.207627457019978 19.965319369816445 23.358659319848957 31.468393853314623 58-59 20.59846903270703 20.38485232211917 31.206007506531137 27.81067113864266 60-61 25.396431545683747 25.399568773743113 23.96157751234927 25.24242216822387 62-63 20.098508961064148 35.77267075076719 18.886398119944786 25.24242216822387 64-65 18.57666814971993 38.22056424472661 17.89646006593883 25.306307539614636 66-67 18.97195888520027 37.81985466078008 17.63920736507067 25.56897908894897 68-69 18.82393876130828 38.20088526871784 17.812610515988457 25.16256545398542 70-71 19.40289993953706 36.33936821930936 18.405831821759815 25.851900019393774 72-73 20.181502903361967 32.74638648368071 19.532381897623694 27.539728715333627 74-75 20.011522183054407 31.6229736358762 20.677470139292925 27.688034041776472 76 20.52431636948561 32.218761764605226 20.12218077278483 27.134741093124337 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 2.5 16 3.5 17 5.0 18 5.0 19 6.5 20 9.5 21 11.0 22 16.0 23 31.0 24 52.0 25 63.0 26 107.5 27 185.5 28 239.0 29 259.0 30 375.0 31 574.0 32 794.5 33 932.0 34 1153.0 35 1504.0 36 1753.5 37 1873.0 38 2300.5 39 3159.0 40 7852.0 41 13877.5 42 15641.0 43 14237.5 44 11166.0 45 8233.5 46 6969.0 47 6494.5 48 5597.0 49 4828.5 50 4483.0 51 4196.0 52 3483.0 53 2827.5 54 2598.0 55 2767.5 56 2928.5 57 3116.0 58 3312.0 59 3633.0 60 4405.0 61 5044.5 62 5233.0 63 5511.0 64 5977.5 65 6351.5 66 6492.0 67 6447.0 68 6388.5 69 6156.5 70 5585.0 71 5187.0 72 4883.0 73 4049.5 74 2961.5 75 2403.0 76 2005.5 77 1206.0 78 543.5 79 283.0 80 180.5 81 47.5 82 10.5 83 4.0 84 2.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 175314.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.50128341147883 #Duplication Level Percentage of deduplicated Percentage of total 1 51.53395695490152 19.325895250807122 2 24.45965472659518 18.345368880979272 3 11.558293406342687 13.003525103528526 4 4.981367404365351 7.4723068323123085 5 2.474712905924405 4.640245502355773 6 1.3232945471138489 2.977514630890859 7 0.9187010419043273 2.411672770001255 8 0.5871168910183284 1.761410954059573 9 0.4365350977260628 1.4733563776994423 >10 1.6852992623013157 9.29532153735583 >50 0.015210282150733894 0.44320476402340947 >100 0.016731310365807286 1.259454464560731 >500 0.0015210282150733894 0.30573713451293105 >1k 0.006084112860293558 7.643428362823277 >5k 0.0 0.0 >10k+ 0.0015210282150733894 9.641557434089691 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 16903 9.641557434089691 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 4273 2.4373409995779003 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 4204 2.3979830475603774 No Hit CACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTTCTG 3773 2.1521384487262853 RNA PCR Primer, Index 34 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 1150 0.6559658669587141 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 536 0.30573713451293105 RNA PCR Primer, Index 24 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT 335 0.19108570907058192 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC 302 0.17226234071437535 No Hit CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 286 0.1631358590871237 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG 228 0.13005236318833635 No Hit CAGCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGC 203 0.11579223564575562 No Hit CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT 203 0.11579223564575562 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001711215305109689 0.0 2 0.0 0.0 0.0 0.0028520255085161482 0.0 3 0.0 0.0 0.0 0.004563240813625837 0.0 4 0.0 0.0 0.0 0.01654174794939366 0.0 5 0.0 0.0 0.0 0.022816204068129186 0.0 6 0.0 0.0 0.0 0.023386609169832415 0.0 7 0.0 0.0 0.0 0.023957014271535643 0.0 8 0.0 0.0 0.0 0.024527419373238876 0.0 9 0.0 0.0 0.0 0.03194268569538086 0.0 10 0.0 0.0 0.0 0.038787546915819614 0.0 11 0.0 0.0 0.0 0.03992835711922607 0.0 12 0.0 0.0 0.0 0.0404987622209293 0.0 13 0.0 0.0 0.0 0.0404987622209293 0.0 14 0.0 0.0 0.0 0.044491597932851915 0.0 15 0.0 0.0 0.0 0.05076605405158744 0.0 16 0.0 0.0 0.0 0.053047674458400355 0.0 17 0.0 0.0 0.0 0.058181320373729424 0.0 18 0.0 0.0 0.0 0.06616699179757464 0.0 19 0.0 0.0 0.0 0.0730118530180134 0.0 20 0.0 0.0 0.0 0.0827087397469683 0.0 21 0.0 0.0 0.0 0.09012400606911028 0.0 22 0.0 0.0 0.0 0.09525765198443935 0.0 23 0.0 0.0 0.0 0.10096170300147164 0.0 24 0.0 0.0 0.0 0.11122899483212978 0.0 25 0.0 0.0 0.0 0.11693304584916207 0.0 26 0.0 0.0 0.0 0.13575641420536866 0.0 27 0.0 0.0 0.0 0.24413338352898228 0.0 28 0.0 0.0 0.0 0.39015708956500905 0.0 29 0.0 0.0 0.0 0.5156462119397196 0.0 30 0.0 0.0 0.0 0.6628107281791529 0.0 31 0.0 0.0 0.0 0.8464811709275928 0.0 32 0.0 0.0 0.0 1.033003639184549 0.0 33 0.0 0.0 0.0 1.303946062493583 0.0 34 0.0 0.0 0.0 1.714067330618205 0.0 35 0.0 0.0 0.0 2.2422624547953958 0.0 36 0.0 0.0 0.0 2.973521795178936 0.0 37 0.0 0.0 0.0 3.9352247966505813 0.0 38 0.0 0.0 0.0 4.8632738971217355 0.0 39 0.0 0.0 0.0 5.756528286388994 0.0 40 0.0 0.0 0.0 6.65491632157158 0.0 41 0.0 0.0 0.0 7.607492841415974 0.0 42 0.0 0.0 0.0 8.605131364294921 0.0 43 0.0 0.0 0.0 9.567975175969973 0.0 44 0.0 0.0 0.0 10.55990964783189 0.0 45 0.0 0.0 0.0 11.501078065642218 0.0 46 0.0 0.0 0.0 12.443957698757657 0.0 47 0.0 0.0 0.0 13.316677504363598 0.0 48 0.0 0.0 0.0 14.111251811036198 0.0 49 0.0 0.0 0.0 14.900122066691765 0.0 50 0.0 0.0 0.0 15.609135608108879 0.0 51 0.0 0.0 0.0 16.31187469340726 0.0 52 0.0 0.0 0.0 17.097322518452604 0.0 53 0.0 0.0 0.0 17.85425008841279 0.0 54 0.0 0.0 0.0 18.672781409356926 0.0 55 0.0 0.0 0.0 19.441687486452878 0.0 56 0.0 0.0 0.0 20.119328747276317 0.0 57 0.0 0.0 0.0 20.79297717238783 0.0 58 0.0 0.0 0.0 21.47860410463511 0.0 59 0.0 0.0 0.0 22.131147540983605 0.0 60 0.0 0.0 0.0 22.742621810009467 0.0 61 0.0 0.0 0.0 23.416840640222688 0.0 62 0.0 0.0 0.0 24.00892113579064 0.0 63 0.0 0.0 0.0 24.60613527727392 0.0 64 0.0 0.0 0.0 25.246129801384942 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTATA 115 0.0 63.91304 2 TCTTTAT 125 0.0 58.800003 1 TTTATAC 135 0.0 57.037037 3 TCGGTAG 20 0.006942263 52.5 9 GTCCTCA 45 9.170022E-5 38.88889 1 TTGAAAA 3410 0.0 36.74487 58 TCGTATG 3465 0.0 36.666668 40 CTCGTAT 3440 0.0 36.627907 39 CGTATGC 3460 0.0 36.618496 41 CTTGAAA 3425 0.0 36.583942 57 GTCTTCT 3420 0.0 36.535088 49 CTGCTTG 3430 0.0 36.530613 54 TATGCCG 3465 0.0 36.46465 43 CTTCTGC 3450 0.0 36.420288 51 GTATGCC 3470 0.0 36.412106 42 TCTCGTA 3355 0.0 36.408344 38 GCTTGAA 3455 0.0 36.367584 56 TCTTCTG 3440 0.0 36.322674 50 CCGTCTT 3440 0.0 36.322674 47 TGCTTGA 3465 0.0 36.262627 55 >>END_MODULE