FastQCFastQC Report
Wed 25 May 2016
SRR1780239_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780239_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386785
Sequences flagged as poor quality0
Sequence length50
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACA301.3058298E-436.636392
AACGCAA300.005716264729.3584636
CAATTAA300.00572350629.35085724
TGTCATA300.005730754729.3432613
TATTTCG300.00574527429.32807427
CGATTAA300.00576346429.30911342
TAGAACG300.005767107529.305324
TCAGCAA508.264062E-526.4294619
CGTCAGA450.001403233524.424262
TTACTCG551.5788904E-424.02678319
GCAAACC551.5928206E-423.99569522
TCAGATG500.002591631121.9789924
TATACCG500.002591631121.9789926
AAATTTG654.9297774E-420.3040529
GTATAGA654.951329E-420.2909221
CTGGTAC550.00449965219.9834863
GCACATA550.00450302119.98090243
AGCAAAC708.049067E-418.87818521
CAGCAAA708.049067E-418.87818520
ACGAGAG708.077163E-418.86840824
Produced by FastQC (version 0.11.5)