FastQCFastQC Report
Wed 25 May 2016
SRR1780239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780239_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386785
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGT173464.484662021536512No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC48871.2634926380288791No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCG43641.1282753984771903No Hit
CACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTTCTG40061.0357175174838735TruSeq Adapter, Index 2 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCC9730.25156094471088586No Hit
ATACACATCTCCGAGCCCACGAGACTGCTGGGTATCTCGTATGCCGTCTT4270.11039724911772689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG4950.039.5527744
TCTTTAT1300.037.2908171
GTCTTCT5300.036.94079243
AGCGAAC250.00235163535.19751727
CCGTCTT5650.034.65242841
CGTCTTC5750.034.0497742
ATGCCGT26000.031.89774944
GTACATG353.183608E-431.4792631
GCCGTCT6700.030.20682540
CTTTATA1550.029.8159142
TACATCT300.005738597429.3350565
AGACTTC300.00574222529.33126411
GTCAAAG300.00574222529.33126422
TAAGACT300.00574222529.3312649
TTTATAC2050.027.9112973
TATGCCG29750.027.87702643
TGTAGAC407.010408E-427.5087282
CTTAATA508.236221E-526.442581
TGCCGTC8250.024.53160339
CCATACA450.001393916324.4522042
Produced by FastQC (version 0.11.5)