FastQCFastQC Report
Wed 25 May 2016
SRR1780238_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780238_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150722
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATAAG200.006940379752.555
CAGTCGG200.006940379752.531
GAAGGTT200.006940379752.525
GGACTAT305.8630673E-446.66666435
TGCCTGA305.8630673E-446.66666411
TTTGAGA305.8630673E-446.66666462
ATGTTAC305.8630673E-446.66666431
TTGGCGC552.981215E-431.8181843
CCAGGAG450.004296707531.1111093
TTACGTC450.004296707531.11110938
GACGAAT450.004296707531.11110925
AGTACGG450.004296707531.1111095
TCTCGGT952.797151E-729.47368244
GGACCGC604.9640134E-429.1666666
CGGACCG604.9640134E-429.1666665
GCTGGGG500.007190816627.99999823
GATTTCG500.007190816627.99999841
TTGAGAA500.007190816627.99999863
GAGCATC500.007190816627.99999840
TGAGAAG500.007190816627.99999864
Produced by FastQC (version 0.11.5)