##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780238_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 150722 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.086848635235732 34.0 27.0 34.0 12.0 34.0 2 29.287058292750892 34.0 27.0 34.0 12.0 34.0 3 29.075954406125184 34.0 27.0 34.0 11.0 34.0 4 29.026943644590702 34.0 27.0 34.0 11.0 34.0 5 29.009918923581164 34.0 27.0 34.0 11.0 34.0 6 31.669192287788114 37.0 27.0 38.0 11.0 38.0 7 31.934694337920146 37.0 28.0 38.0 12.0 38.0 8 32.0279123153886 37.0 31.0 38.0 11.0 38.0 9 32.20836374251934 38.0 31.0 38.0 12.0 38.0 10-11 31.900654847998304 37.5 29.5 38.0 11.0 38.0 12-13 31.943634638606177 38.0 30.0 38.0 11.0 38.0 14-15 31.781140112259656 37.0 27.5 38.0 11.0 38.0 16-17 31.80241437879009 38.0 28.5 38.0 11.0 38.0 18-19 31.84122424065498 38.0 29.0 38.0 11.0 38.0 20-21 31.842179641989887 38.0 29.5 38.0 11.0 38.0 22-23 31.830714825970993 38.0 29.0 38.0 11.0 38.0 24-25 31.860478231445974 38.0 29.5 38.0 11.0 38.0 26-27 31.803412905879696 38.0 28.0 38.0 11.0 38.0 28-29 31.834931197834422 38.0 28.5 38.0 11.0 38.0 30-31 31.858401560488847 38.0 28.5 38.0 11.0 38.0 32-33 31.921826276190604 38.0 30.0 38.0 11.0 38.0 34-35 31.84554676822229 38.0 28.0 38.0 11.0 38.0 36-37 31.80038746831916 38.0 27.0 38.0 11.0 38.0 38-39 31.754203102400446 38.0 27.0 38.0 11.0 38.0 40-41 31.81280768567296 38.0 27.0 38.0 11.0 38.0 42-43 31.899639734079962 38.0 28.0 38.0 11.0 38.0 44-45 31.9807957696952 38.0 30.5 38.0 11.0 38.0 46-47 31.872865275142317 38.0 29.0 38.0 11.0 38.0 48-49 31.81249253592707 38.0 27.0 38.0 11.0 38.0 50-51 31.768978649434057 38.0 27.0 38.0 11.0 38.0 52-53 31.879616114435848 38.0 27.0 38.0 11.0 38.0 54-55 31.83713724605565 38.0 27.0 38.0 11.0 38.0 56-57 31.7874895502979 38.0 27.0 38.0 11.0 38.0 58-59 31.805114714507503 38.0 27.0 38.0 11.0 38.0 60-61 31.832615676543572 38.0 27.0 38.0 11.0 38.0 62-63 31.699542867000172 38.0 27.0 38.0 11.0 38.0 64-65 31.63904738525232 37.5 27.0 38.0 11.0 38.0 66-67 31.568679423043747 37.0 27.0 38.0 11.0 38.0 68-69 31.475322116213956 37.0 27.0 38.0 11.0 38.0 70-71 31.426271546290522 37.0 27.0 38.0 11.0 38.0 72-73 31.363324531256218 37.0 26.5 38.0 11.0 38.0 74-75 31.334692347500695 37.0 26.0 38.0 11.0 38.0 76 29.879188174254587 36.0 24.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.25386849156976155 1101 2 -0.49794001760735895 1101 3 -0.2785593440748002 1101 4 0.1261404252123448 1101 5 -0.4286043732199438 1101 6 -0.34186069240825034 1101 7 -0.5479253479019981 1101 8 -0.8590181140109152 1101 9 -0.09218782184031227 1101 10-11 -0.5205403305096539 1101 12-13 -0.4494767968534248 1101 14-15 -0.45216236994525616 1101 16-17 -0.105324012581967 1101 18-19 -0.3102201743331854 1101 20-21 -0.1727759049331894 1101 22-23 -0.11363965897117012 1101 24-25 0.030950610764922004 1101 26-27 -0.2099522511791001 1101 28-29 -0.30914295384356905 1101 30-31 -0.4713969584048705 1101 32-33 -0.37641883002050847 1101 34-35 -0.41620957129272895 1101 36-37 -0.32658226305107974 1101 38-39 -0.26345889603534545 1101 40-41 -0.11412901972865797 1101 42-43 -0.48143336633163614 1101 44-45 -0.3297999902482047 1101 46-47 -0.45338389547734437 1101 48-49 -0.6075909012570442 1101 50-51 -0.36988136842146346 1101 52-53 -0.690803661383363 1101 54-55 -0.16586586151508698 1101 56-57 -0.10455388627603313 1101 58-59 -0.22466214322204436 1101 60-61 -0.5379841846964588 1101 62-63 -0.5665869255057139 1101 64-65 -0.6596480079786637 1101 66-67 -0.7239068960773452 1101 68-69 -0.7683948083165681 1101 70-71 -1.0464207266609478 1101 72-73 -0.3429966369081079 1101 74-75 -0.4959207019022429 1101 76 -1.3819438618578168 1102 1 0.0381972435011555 1102 2 0.3249521161656048 1102 3 0.04278885292106338 1102 4 -0.20813497123921465 1102 5 -0.07063347250293006 1102 6 -0.09229247133743712 1102 7 -0.397954133163946 1102 8 -0.4749049617630483 1102 9 -0.11694314711377274 1102 10-11 -0.08827074344732466 1102 12-13 0.41571658777000664 1102 14-15 -0.31025102855205233 1102 16-17 -0.40714402618954537 1102 18-19 -0.6781677851564432 1102 20-21 -0.27239907968589705 1102 22-23 -0.04781954068991112 1102 24-25 -0.15020027634451338 1102 26-27 -0.3117408626827398 1102 28-29 0.06774117239288913 1102 30-31 -0.059492944169249995 1102 32-33 -0.6188234461297917 1102 34-35 -0.3683017364677923 1102 36-37 -0.23393297221526055 1102 38-39 -0.049283462947997236 1102 40-41 -0.21981672580496792 1102 42-43 -0.4380657523681677 1102 44-45 -0.3218513326881478 1102 46-47 -0.5549239069914513 1102 48-49 -0.1574940781032268 1102 50-51 -0.48880375288788613 1102 52-53 0.06622910147841665 1102 54-55 -0.525099388494727 1102 56-57 -0.2890804995591232 1102 58-59 -0.3656248793920369 1102 60-61 -0.36760814450176227 1102 62-63 -0.029126832346136666 1102 64-65 -0.2103166111084036 1102 66-67 -0.07052724464070081 1102 68-69 -0.02302007654287408 1102 70-71 0.17850338492119633 1102 72-73 -0.05068884395264561 1102 74-75 -0.10356449736987372 1102 76 0.025668531505871783 1103 1 0.7487221820053698 1103 2 0.6134589461232132 1103 3 0.5711815885676863 1103 4 0.7453114096683038 1103 5 0.9677686837748745 1103 6 0.19699941121869458 1103 7 0.6360124759321977 1103 8 0.9259559792533381 1103 9 1.1435634734964708 1103 10-11 0.5701332446198748 1103 12-13 1.1114040321621133 1103 14-15 0.6436925523345352 1103 16-17 0.9555568164335746 1103 18-19 0.7079145406927232 1103 20-21 0.8039080328906465 1103 22-23 1.23610127367132 1103 24-25 0.7654065693141447 1103 26-27 0.5555917902716772 1103 28-29 0.7711679269854521 1103 30-31 0.6969968239785551 1103 32-33 0.8904205482178291 1103 34-35 0.717210117826447 1103 36-37 0.8184954571561747 1103 38-39 0.566851984793665 1103 40-41 0.7498606175770348 1103 42-43 0.9019863227875327 1103 44-45 0.7038787662284776 1103 46-47 0.7849580734345771 1103 48-49 0.9623572852714553 1103 50-51 0.5731238129256901 1103 52-53 0.8273310536425456 1103 54-55 0.9481237757906129 1103 56-57 1.155808808024485 1103 58-59 0.707980343824584 1103 60-61 1.0941401676351497 1103 62-63 0.9541384630953225 1103 64-65 1.1097820438348052 1103 66-67 1.1802381816428635 1103 68-69 1.4077709947922443 1103 70-71 1.325340931370139 1103 72-73 0.7593349693095099 1103 74-75 1.2126285208956773 1103 76 0.7631338583494376 1104 1 -0.17139871609480295 1104 2 0.3981844393979266 1104 3 -0.04559498546277396 1104 4 0.28176401865977496 1104 5 -0.11103751290697161 1104 6 -0.07209045113541634 1104 7 -0.21108544629525738 1104 8 -0.7956120324701175 1104 9 -0.24068052085114644 1104 10-11 -0.08700811718469481 1104 12-13 -0.6398389677855434 1104 14-15 -0.31908941239043287 1104 16-17 -0.2783561474016665 1104 18-19 -0.12513748212613862 1104 20-21 -0.5779546352414542 1104 22-23 -0.4202942881646585 1104 24-25 -0.44312956927380753 1104 26-27 -0.20315500409687814 1104 28-29 -0.12417801952630114 1104 30-31 -0.3271697118460146 1104 32-33 -0.29811637542271896 1104 34-35 -0.2622411304071903 1104 36-37 -0.47509458837687646 1104 38-39 -0.008879422543959237 1104 40-41 -0.3965844025726426 1104 42-43 -0.3067526210550362 1104 44-45 -0.398871534708352 1104 46-47 -0.22537845244599453 1104 48-49 -0.4175950882042372 1104 50-51 -0.3953694094535436 1104 52-53 -0.332760797511483 1104 54-55 -0.26752363091896925 1104 56-57 -0.21079767127629623 1104 58-59 -0.45905922282637945 1104 60-61 -0.4016990535926759 1104 62-63 -0.14907632729563502 1104 64-65 -0.10551863131042438 1104 66-67 -0.2611838102972648 1104 68-69 -0.1417069452297426 1104 70-71 -0.18765823124042313 1104 72-73 -0.0633151065789086 1104 74-75 -0.43563520444057957 1104 76 -0.284937529100187 1105 1 -0.14585432748508964 1105 2 -0.3867884046201411 1105 3 -0.8622159691691849 1105 4 -0.41317532452077543 1105 5 -0.4147657500522932 1105 6 -0.3066978314714426 1105 7 -0.2703717769383722 1105 8 -0.1787905513270296 1105 9 -0.373503727794958 1105 10-11 0.5119081814803508 1105 12-13 -0.9717383637552928 1105 14-15 -0.0588551603220111 1105 16-17 -0.15161765696970875 1105 18-19 -0.22918676896437162 1105 20-21 -0.4434817658439627 1105 22-23 -0.2855549348323372 1105 24-25 -0.045890850427049656 1105 26-27 -0.2591229752658535 1105 28-29 -0.40387828854818153 1105 30-31 0.13246431688348892 1105 32-33 0.3984789750671034 1105 34-35 0.21109144154347348 1105 36-37 -0.27978089871645295 1105 38-39 -0.019297675424525096 1105 40-41 0.17551219150887576 1105 42-43 0.45778344310414454 1105 44-45 -0.14819898001569598 1105 46-47 0.5213602917801872 1105 48-49 -0.13928434255680955 1105 50-51 0.20194798560254767 1105 52-53 -0.0781353977855801 1105 54-55 -0.16714167061238783 1105 56-57 -0.0417072697738341 1105 58-59 -0.12186187243807467 1105 60-61 0.09358037384755491 1105 62-63 0.2263805023069274 1105 64-65 -0.04175283262000562 1105 66-67 0.15394740131635842 1105 68-69 0.1466857759519229 1105 70-71 0.15002132817280867 1105 72-73 0.05827136111308917 1105 74-75 -0.08474565568671011 1105 76 0.33287374951510884 1106 1 -0.06539535757255521 1106 2 -0.321487086948661 1106 3 0.32970079348539727 1106 4 0.07447704311083214 1106 5 -0.5567350423576478 1106 6 -0.49447551080007557 1106 7 -0.37430453898538474 1106 8 0.014835007196069228 1106 9 -0.104488627102576 1106 10-11 -1.1255865971989465 1106 12-13 0.029435642295108977 1106 14-15 -0.04065964875915995 1106 16-17 -0.15878194187028427 1106 18-19 -0.21885488283346533 1106 20-21 -0.026921635216012874 1106 22-23 -0.21484559468069975 1106 24-25 -0.43418623161510084 1106 26-27 -0.37604575035034316 1106 28-29 -0.22659704606371633 1106 30-31 -0.41541361180276226 1106 32-33 -0.02513784961044152 1106 34-35 0.32325806812727365 1106 36-37 -0.06673835602820333 1106 38-39 -0.1885349195798085 1106 40-41 0.06335822267224955 1106 42-43 -0.5601715712623978 1106 44-45 -0.3853102289556105 1106 46-47 -0.04565298619660041 1106 48-49 -0.252270558270137 1106 50-51 -0.23134885122643922 1106 52-53 -0.08736922434912842 1106 54-55 0.008234580922366064 1106 56-57 -0.15059870427632305 1106 58-59 -0.27744140739244116 1106 60-61 -0.20633228769694156 1106 62-63 -0.40734859658805433 1106 64-65 -0.2177619715134611 1106 66-67 0.030028056481349097 1106 68-69 0.22766371349860037 1106 70-71 0.016871321211045398 1106 72-73 -0.09556773360483461 1106 74-75 -0.21881651375539235 1106 76 -0.2636668881821933 1107 1 -0.0177136239675022 1107 2 -0.27121051517290695 1107 3 -0.020507426455615985 1107 4 0.17727693678575562 1107 5 0.30382721477182884 1107 6 -0.6659943138835018 1107 7 0.053748826076699174 1107 8 0.5448275391252295 1107 9 0.2847877294914767 1107 10-11 0.3196971262039625 1107 12-13 0.35866364629495706 1107 14-15 0.4625016235975892 1107 16-17 0.13607078524134764 1107 18-19 0.3097579546537155 1107 20-21 -0.005039554788684342 1107 22-23 0.18555059280584985 1107 24-25 -0.07352904138182126 1107 26-27 0.013490090060777504 1107 28-29 0.08732774160257506 1107 30-31 -0.28462111025218917 1107 32-33 -0.06885770834998084 1107 34-35 -0.017551774536926246 1107 36-37 -0.3187256223345436 1107 38-39 -0.29065445934411827 1107 40-41 0.2860649553279728 1107 42-43 -0.24196530061444932 1107 44-45 -0.1582492312719772 1107 46-47 -0.035172371536393854 1107 48-49 -0.5551134882854534 1107 50-51 -0.3508158334929341 1107 52-53 -0.25275851336346733 1107 54-55 0.21602415853334378 1107 56-57 -0.2992449582160468 1107 58-59 0.0678366200242344 1107 60-61 -0.18028897288611034 1107 62-63 -0.22336824141166645 1107 64-65 -0.05934077231849599 1107 66-67 -0.19781139265349168 1107 68-69 0.07130889152981368 1107 70-71 -0.00198872207844758 1107 72-73 0.17510553196736112 1107 74-75 0.15520409305700866 1107 76 -0.3681312756105193 1108 1 -0.6158431605392494 1108 2 0.2163662575797467 1108 3 0.01753632766853741 1108 4 0.16055189744765386 1108 5 -0.21709811896757714 1108 6 -0.3725955016404683 1108 7 -0.14290362811344082 1108 8 -0.2223797092377957 1108 9 -0.5664380966087244 1108 10-11 -0.3370081171846948 1108 12-13 -0.07923290717948461 1108 14-15 -0.3822207255217478 1108 16-17 -0.19881069285621322 1108 18-19 0.026377669389010094 1108 20-21 -0.09310615039296977 1108 22-23 -0.13620337907374847 1108 24-25 -0.18555381169804974 1108 26-27 -0.5087105596524353 1108 28-29 -0.46761236296064723 1108 30-31 -0.16934142901773086 1108 32-33 -0.3928133451196878 1108 34-35 -0.36703911020516955 1108 36-37 0.038794300512012825 1108 38-39 0.06182764816311348 1108 40-41 -0.5847157157039575 1108 42-43 0.049307985005572874 1108 44-45 -0.32563921147603025 1108 46-47 -0.09280269487023673 1108 48-49 -0.27491832052747256 1108 50-51 -0.1504199145040488 1108 52-53 -0.23175069650137914 1108 54-55 -0.5389882773836163 1108 56-57 -0.3825148429934657 1108 58-59 -0.06890770767486387 1108 60-61 -0.6251839020775201 1108 62-63 -0.4811470343663409 1108 64-65 -0.4060236818154763 1108 66-67 -0.07431512342857616 1108 68-69 -0.33236351088630656 1108 70-71 -0.28740570598789716 1108 72-73 -0.20599187425567322 1108 74-75 -0.22730187110724742 1108 76 -0.9617052058678652 1109 1 0.2610617992834179 1109 2 -0.21914219351474173 1109 3 -0.35491637894875794 1109 4 -0.048571758060042214 1109 5 -0.02303151779374346 1109 6 0.004133131322578976 1109 7 0.5270943567224506 1109 8 0.5578477524111136 1109 9 0.5681496763830367 1109 10-11 0.29683656404152714 1109 12-13 -0.050038217136915364 1109 14-15 0.42326241007360466 1109 16-17 0.23624434631353708 1109 18-19 0.2638175293351033 1109 20-21 0.2781563499219004 1109 22-23 0.36727592770980166 1109 24-25 -0.04100357113783204 1109 26-27 -0.2946466033814943 1109 28-29 -0.2970003828100154 1109 30-31 -0.2278284186144468 1109 32-33 -0.16753688979335735 1109 34-35 0.11984343091677374 1109 36-37 -0.011036929493783987 1109 38-39 0.1803570821538898 1109 40-41 -0.038763676609775644 1109 42-43 -0.1958713646002117 1109 44-45 0.03733592643676786 1109 46-47 -0.04519129407928446 1109 48-49 0.09778943029748888 1109 50-51 -0.14579133443906045 1109 52-53 -0.2701616136529452 1109 54-55 0.12369844016594556 1109 56-57 0.1408106815789374 1109 58-59 -0.11115058532921296 1109 60-61 -0.21765159647788224 1109 62-63 0.14351605538402978 1109 64-65 0.2665436372605683 1109 66-67 -0.1288756917048559 1109 68-69 0.15679868463498892 1109 70-71 -0.11785483568889887 1109 72-73 -0.2229696318818135 1109 74-75 0.12891616964944674 1109 76 -0.5013409649549949 1110 1 -0.2505653827614722 1110 2 0.038588479801969555 1110 3 0.06432925696146796 1110 4 -0.1336652742695179 1110 5 -0.15045670482616202 1110 6 0.15854086199589545 1110 7 0.3259347557249441 1110 8 0.6468627150046302 1110 9 -0.009998702669328452 1110 10-11 -0.04766468284126546 1110 12-13 0.10718123423465897 1110 14-15 0.14252674922572695 1110 16-17 0.20635344855793036 1110 18-19 0.19155443706577913 1110 20-21 0.33339637485955365 1110 22-23 0.4553370249666564 1110 24-25 0.5086254812312418 1110 26-27 0.4317187332768597 1110 28-29 0.7554684451201616 1110 30-31 0.24250200532570076 1110 32-33 0.6168583720520324 1110 34-35 0.3759406877746301 1110 36-37 0.18732965404736746 1110 38-39 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-0.629689378093488 2116 6 -0.37529577166747075 2116 7 0.02311297354672348 2116 8 -0.42189966123299527 2116 9 -0.5759640492039821 2116 10-11 -0.3531597323462137 2116 12-13 -0.64573955784455 2116 14-15 -0.39262176562575846 2116 16-17 -0.5227430860955344 2116 18-19 -0.5747574441223406 2116 20-21 -0.2287447142493555 2116 22-23 -0.2940441631521544 2116 24-25 -0.6339611524321214 2116 26-27 -0.2269198805845285 2116 28-29 -0.4998780895333006 2116 30-31 -0.5543049253673686 2116 32-33 -0.39098816260143465 2116 34-35 -0.39455436173032155 2116 36-37 -0.659650543972436 2116 38-39 -0.3457256071624215 2116 40-41 -0.6087681209444824 2116 42-43 -0.7754619919921275 2116 44-45 -0.4630529528501661 2116 46-47 -0.2790713217329248 2116 48-49 0.030599731277710873 2116 50-51 -0.3188117536879673 2116 52-53 -0.4568607075024822 2116 54-55 -0.473444222978177 2116 56-57 -0.6645305445636218 2116 58-59 -0.3431726341675123 2116 60-61 -0.3868850721945343 2116 62-63 -0.5891078304459505 2116 64-65 -0.5336989493422308 2116 66-67 -0.21071626354258655 2116 68-69 -0.07952572710792793 2116 70-71 -0.3483367990972077 2116 72-73 -0.24508328339658902 2116 74-75 -0.35118925984612304 2116 76 -0.34296833218049727 2117 1 -0.29364913910664825 2117 2 -0.9129343628376247 2117 3 -0.22322150445985045 2117 4 0.06751492877616627 2117 5 -0.7001648266535732 2117 6 -0.4010911176995364 2117 7 -0.28317691940196 2117 8 -0.9009291631679588 2117 9 -0.4999098701820195 2117 10-11 0.022462220712036185 2117 12-13 -0.13451927337955638 2117 14-15 0.35838969338816185 2117 16-17 -0.3010513737770957 2117 18-19 0.11194783808100794 2117 20-21 -0.6579551479830812 2117 22-23 -0.04371119838360116 2117 24-25 -0.33171081318499773 2117 26-27 -0.2544868504794877 2117 28-29 -0.4456990675701924 2117 30-31 0.41918982258458826 2117 32-33 0.24948527560532696 2117 34-35 0.4322097233076292 2117 36-37 -0.23584293478512208 2117 38-39 0.16027404102575105 2117 40-41 -0.20545355090879625 2117 42-43 -0.12219768078943716 2117 44-45 -0.4871143691440807 2117 46-47 -0.1611703818927488 2117 48-49 0.20989811796917124 2117 50-51 0.5558175874187334 2117 52-53 -0.05443823175236773 2117 54-55 0.24401946501498628 2117 56-57 -0.09987883827624344 2117 58-59 -0.24130426205111277 2117 60-61 -0.2751031452708794 2117 62-63 -0.027075865828166457 2117 64-65 -0.10521098633272885 2117 66-67 -0.2731691458866621 2117 68-69 -0.29977878033203353 2117 70-71 -0.4703903750131744 2117 72-73 -1.4551714876484851 2117 74-75 -0.5134373374457581 2117 76 -1.185670749630365 2118 1 0.3991795151977122 2118 2 0.08399664306727317 2118 3 -0.43072176344669444 2118 4 -0.3861652742695192 2118 5 -0.21367099054044658 2118 6 -0.2206938318816576 2118 7 0.07256740878617052 2118 8 -0.5838515707096548 2118 9 -0.39953951899586215 2118 10-11 -1.008532029780035 2118 12-13 -0.7005738678061597 2118 14-15 -0.5069630466926398 2118 16-17 -0.581631245319624 2118 18-19 -0.14982311395462844 2118 20-21 -0.5729556659567692 2118 22-23 -0.35588746482926226 2118 24-25 -0.401553090197325 2118 26-27 -0.37394453202926314 2118 28-29 -0.02050094263493918 2118 30-31 -0.19035513753144073 2118 32-33 -0.09783550549899189 2118 34-35 -0.1698246183478176 2118 36-37 -0.5069050398301869 2118 38-39 -0.6127239906742403 2118 40-41 -0.26874993545246184 2118 42-43 -0.3668819758272477 2118 44-45 -0.08008417127813061 2118 46-47 -0.6830418208236502 2118 48-49 -0.29906282681483276 2118 50-51 -0.37104452798580567 2118 52-53 -0.264347068383465 2118 54-55 -0.39355434623539765 2118 56-57 -0.07711389473537622 2118 58-59 -0.10933751596181551 2118 60-61 -0.15869503380293892 2118 62-63 5.0623300121444E-4 2118 64-65 -0.18398183477362906 2118 66-67 -0.2929298420432964 2118 68-69 -0.560228899465983 2118 70-71 -0.4779463161713622 2118 72-73 -0.6214345664840799 2118 74-75 -0.4338701044611959 2118 76 -0.32518696220676446 2119 1 -0.32640735167338875 2119 2 -0.49794001760735895 2119 3 -0.6852950953701367 2119 4 0.15981918168903064 2119 5 -0.2653919379867844 2119 6 -0.34704203955850943 2119 7 -0.7733139489382701 2119 8 -0.004095834218169614 2119 9 -0.024830308886947705 2119 10-11 -0.36639525278944873 2119 12-13 -0.5375596984078292 2119 14-15 -0.3783281730540651 2119 16-17 -0.18693023019854849 2119 18-19 -0.8218782054212674 2119 20-21 -1.0199261640005446 2119 22-23 -1.1123443221836027 2119 24-25 -0.549360270064092 2119 26-27 -0.7941491423708094 2119 28-29 -0.6873812958124823 2119 30-31 -0.5426404817209303 2119 32-33 -0.3414447367562623 2119 34-35 -0.3915981723289974 2119 36-37 -0.5947169780769883 2119 38-39 -0.8411791032892353 2119 40-41 -0.6931445637701117 2119 42-43 -0.4607079777305998 2119 44-45 -0.9593336690046854 2119 46-47 -0.8083061752700935 2119 48-49 -0.9417877924487534 2119 50-51 -0.6017466533955549 2119 52-53 -0.9200782727823267 2119 54-55 -0.9301145661783004 2119 56-57 -0.5086989639962418 2119 58-59 -0.6948693971080573 2119 60-61 -0.5211448064581141 2119 62-63 -0.6559651638476822 2119 64-65 -0.8539485261133777 2119 66-67 -0.837896533383045 2119 68-69 -0.9212445492492094 2119 70-71 -0.362482902826752 2119 72-73 -0.4660536317267585 2119 74-75 -0.6746771785861796 2119 76 -0.6850526701479716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 79.0 13 671.0 14 2207.0 15 3383.0 16 4317.0 17 5218.0 18 5504.0 19 4940.0 20 3804.0 21 2821.0 22 2041.0 23 1609.0 24 1312.0 25 1136.0 26 1168.0 27 1267.0 28 1471.0 29 1764.0 30 2087.0 31 2721.0 32 3254.0 33 4418.0 34 6030.0 35 8894.0 36 16789.0 37 61817.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.62400976632476 21.142898846883668 14.568543411048154 29.66454797574342 2 17.97149719350858 19.854433991056382 35.098393068032536 27.0756757474025 3 17.69880972917026 21.776515704409444 27.91430580804395 32.610368758376346 4 14.12866071310094 21.68362946351561 29.548440174626133 34.63926964875731 5 14.742373376149468 34.18943485357148 30.74932657475352 20.318865195525536 6 28.8677167235042 31.540186568649563 17.510383354785635 22.081713353060604 7 25.744919420651403 26.09390861260209 21.8755183418369 26.2856536249096 8 22.508326588022985 32.795477767014766 19.489523758973473 25.206671885988776 9 22.144079829089318 18.63231645015326 19.936041188413107 39.28756253234432 10-11 25.087578455699898 23.909250142646727 24.462918485688885 26.54025291596449 12-13 23.830628574461592 21.060959912952324 24.375008293414364 30.733403219171716 14-15 23.462069240057858 22.218057085229763 22.3142606918698 32.005612982842585 16-17 24.08706094664349 23.538700388795267 22.748503868048463 29.625734796512788 18-19 23.09292304017675 22.62948550803966 24.025437644927898 30.25215380685569 20-21 23.629674598514477 23.042167175884 24.001552532319543 29.326605693281984 22-23 24.951566460105358 22.769403272249573 22.879539815023687 29.399490452621386 24-25 23.556945900399413 23.95038547789971 22.280091824683858 30.212576797017025 26-27 23.121044041347645 23.956688472817504 23.684664481628428 29.23760300420642 28-29 23.593768660182324 23.510502779952493 24.954552089276945 27.941176470588236 30-31 23.447472830774537 22.569034381178593 25.069332944095752 28.914159843951115 32-33 22.730590093018936 23.57718183145128 24.9761149666273 28.71611310890248 34-35 23.14725123074269 22.80124998341317 25.396093470097263 28.655405315746872 36-37 23.399039290879898 21.5894162763233 25.98990193866854 29.021642494128265 38-39 22.101617547537852 21.765568397446955 27.924257905282573 28.208556149732622 40-41 21.86807499900479 21.782486962752618 27.91496928119319 28.4344687570494 42-43 20.473786175873464 21.74367378352198 27.987619591035152 29.794920449569407 44-45 19.798370509945464 21.346916840275473 29.61876832844575 29.235944321333317 46-47 20.23626278844495 22.43833017077799 29.26480540332533 28.060601637451732 48-49 19.896232799458605 22.70073380130306 28.978848476002177 28.424184923236158 50-51 19.787091466408356 23.675044120964426 27.5248470694391 29.013017343188118 52-53 18.44753917808946 24.19255317737291 27.67910457663778 29.68080306789984 54-55 18.808468571276922 23.519459667467256 27.44987460357479 30.22219715768103 56-57 19.226788391873782 23.768262098432878 26.944971537001898 30.059977972691442 58-59 19.534372999207147 23.602472109154967 26.654458720222397 30.20869617141549 60-61 19.295789599394915 22.687132601743606 26.976486511590874 31.0405912872706 62-63 19.363132124042938 22.91039131646342 26.516036146017168 31.210440413476466 64-65 20.040869945993286 23.234166213293346 25.62864080890646 31.096323031806904 66-67 19.603309404068415 24.12322023327716 24.139143588858957 32.134326773795465 68-69 20.09029869561179 24.642056235984132 23.911904035243694 31.35574103316039 70-71 20.9395443266411 24.024694470614776 24.144451374052892 30.89130982869123 72-73 20.80499731291592 23.072763583044168 25.664970375727336 30.457268728312574 74-75 21.143562320032906 22.843712264964637 25.934170194132243 30.07855522087021 76 20.760738312920477 22.163984023566567 26.152121123658123 30.923156539854833 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 2.5 14 6.5 15 9.0 16 12.0 17 14.0 18 20.0 19 27.0 20 36.5 21 45.0 22 63.0 23 109.5 24 163.5 25 189.0 26 270.5 27 406.5 28 605.0 29 749.0 30 833.0 31 1108.0 32 1550.5 33 1802.0 34 2100.5 35 2691.5 36 3224.5 37 3465.0 38 3898.5 39 4846.0 40 5873.0 41 6665.5 42 6945.0 43 7113.0 44 7515.0 45 7739.0 46 7729.0 47 7742.5 48 7513.5 49 6896.0 50 6521.0 51 6005.0 52 5135.5 53 4496.5 54 4211.0 55 4072.5 56 3744.5 57 3478.5 58 3402.0 59 3400.0 60 3534.0 61 3875.0 62 4080.0 63 4121.0 64 4235.5 65 4255.0 66 4126.0 67 4051.0 68 3821.5 69 3401.5 70 2986.5 71 2762.0 72 2444.5 73 1837.0 74 1245.0 75 943.0 76 716.5 77 345.5 78 136.0 79 71.0 80 56.5 81 31.0 82 16.0 83 12.0 84 12.0 85 13.0 86 8.5 87 3.0 88 9.0 89 13.0 90 10.5 91 8.5 92 7.0 93 7.5 94 7.0 95 6.5 96 7.0 97 6.5 98 8.0 99 29.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 6.634731492416502E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.317365746208251E-4 20-21 3.317365746208251E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.317365746208251E-4 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.634731492416502E-4 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 150722.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.41623651490824 #Duplication Level Percentage of deduplicated Percentage of total 1 75.70555407919214 47.25255768899033 2 11.886260961998406 14.837913509640266 3 5.626361945256445 10.535290136808163 4 2.559659845867659 6.390573373495574 5 1.3212862078129153 4.1234856225368555 6 0.8025511559925591 3.0055333660646752 7 0.5346797767738507 2.3360889584798503 8 0.35716183895827797 1.7834158251615557 9 0.2848790858357693 1.6002972359708605 >10 0.9205421206484188 8.098353259643583 >50 0.0010629816635663034 0.036491023208290764 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012605989835591353 0.0 2 0.0 0.0 0.0 0.014596409283316304 0.0 3 0.0 0.0 0.0 0.014596409283316304 0.0 4 0.0 0.0 0.0 0.03649102320829076 0.0 5 0.0 0.0 0.0 0.043125754700707265 0.0 6 0.0 0.0 0.0 0.04378922784994891 0.0 7 0.0 0.0 0.0 0.04378922784994891 0.0 8 0.0 0.0 0.0 0.04378922784994891 0.0 9 0.0 0.0 0.0 0.05639521768554027 0.0 10 0.0 0.0 0.0 0.06435689547644007 0.0 11 0.0 0.0 0.0 0.06435689547644007 0.0 12 0.0 0.0 0.0 0.06502036862568172 0.0 13 0.0 0.0 0.0 0.06701078807340667 0.0 14 0.0 6.634731492416502E-4 0.0 0.06900120752113162 0.0 15 0.0 6.634731492416502E-4 0.0 0.06966468067037326 0.0 16 0.0 6.634731492416502E-4 0.0 0.06966468067037326 0.0 17 0.0 6.634731492416502E-4 0.0 0.07099162696885657 0.0 18 0.0 6.634731492416502E-4 0.0 0.07696288531203142 0.0 19 0.0 6.634731492416502E-4 0.0 0.08028025105823967 0.0 20 0.0 6.634731492416502E-4 0.0 0.08492456310293123 0.0 21 0.0 6.634731492416502E-4 0.0 0.08757845569989782 0.0 22 0.0 6.634731492416502E-4 0.0 0.09686707978928093 0.0 23 0.0 6.634731492416502E-4 0.0 0.10482875758018073 0.0 24 0.0 6.634731492416502E-4 0.0 0.11345390852032218 0.0 25 0.0 0.0013269462984833004 0.0 0.12340600575894693 0.0 26 0.0 0.0013269462984833004 0.0 0.13203115669908838 0.0 27 0.0 0.0013269462984833004 0.0 0.2474754846671355 0.0 28 0.0 0.0013269462984833004 0.0 0.3841509534109155 0.0 29 0.0 0.0013269462984833004 0.0 0.5241437879009037 0.0 30 0.0 0.0013269462984833004 0.0 0.644895901062884 0.0 31 0.0 0.0013269462984833004 0.0 0.7795809503589389 0.0 32 0.0 0.0013269462984833004 0.0 0.9354971404307267 0.0 33 0.0 0.0013269462984833004 0.0 1.196905561231937 0.0 34 0.0 0.0013269462984833004 0.0 1.5671235785087778 0.0 35 0.0 0.0013269462984833004 0.0 2.036862568171866 0.0 36 0.0 0.0013269462984833004 0.0 2.6366422950863178 0.0 37 0.0 0.0013269462984833004 0.0 3.483897506667905 0.0 38 0.0 0.0013269462984833004 0.0 4.3291622988017675 0.0 39 0.0 0.0013269462984833004 0.0 5.169119305741696 6.634731492416502E-4 40 0.0 0.0013269462984833004 0.0 6.019691883069492 6.634731492416502E-4 41 0.0 0.0013269462984833004 0.0 6.845715953875347 6.634731492416502E-4 42 0.0 0.0013269462984833004 0.0 7.733443027560675 6.634731492416502E-4 43 0.0 0.0013269462984833004 0.0 8.606573691962685 6.634731492416502E-4 44 0.0 0.0013269462984833004 0.0 9.522830111065405 6.634731492416502E-4 45 0.0 0.0013269462984833004 0.0 10.46960629503324 6.634731492416502E-4 46 0.0 0.0013269462984833004 0.0 11.389180079882166 6.634731492416502E-4 47 0.0 0.0013269462984833004 0.0 12.17804965433049 6.634731492416502E-4 48 0.0 0.0013269462984833004 0.0 12.902562333302372 6.634731492416502E-4 49 0.0 0.0013269462984833004 0.0 13.671527713273443 6.634731492416502E-4 50 0.0 0.0013269462984833004 0.0 14.352914637544618 6.634731492416502E-4 51 0.0 0.0013269462984833004 0.0 14.990512333965844 6.634731492416502E-4 52 0.0 0.0013269462984833004 0.0 15.710380700893035 6.634731492416502E-4 53 0.0 0.0013269462984833004 0.0 16.414325712238426 6.634731492416502E-4 54 0.0 0.0013269462984833004 0.0 17.101020421703534 6.634731492416502E-4 55 0.0 0.0013269462984833004 0.0 17.808946271944375 6.634731492416502E-4 56 0.0 0.0013269462984833004 0.0 18.457823011902708 6.634731492416502E-4 57 0.0 0.0013269462984833004 0.0 19.09674765462242 6.634731492416502E-4 58 0.0 0.0013269462984833004 0.0 19.705152532477012 6.634731492416502E-4 59 0.0 0.0013269462984833004 0.0 20.361990950226243 6.634731492416502E-4 60 0.0 0.0013269462984833004 0.0 20.93523175117103 6.634731492416502E-4 61 0.0 0.0013269462984833004 0.0 21.523068961399133 6.634731492416502E-4 62 0.0 0.0013269462984833004 0.0 22.12550258091055 6.634731492416502E-4 63 0.0 0.0013269462984833004 0.0 22.71997452263107 6.634731492416502E-4 64 0.0 0.0013269462984833004 0.0 23.29520574302358 6.634731492416502E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATAAG 20 0.0069403797 52.5 55 CAGTCGG 20 0.0069403797 52.5 31 GAAGGTT 20 0.0069403797 52.5 25 GGACTAT 30 5.8630673E-4 46.666664 35 TGCCTGA 30 5.8630673E-4 46.666664 11 TTTGAGA 30 5.8630673E-4 46.666664 62 ATGTTAC 30 5.8630673E-4 46.666664 31 TTGGCGC 55 2.981215E-4 31.818184 3 CCAGGAG 45 0.0042967075 31.111109 3 TTACGTC 45 0.0042967075 31.111109 38 GACGAAT 45 0.0042967075 31.111109 25 AGTACGG 45 0.0042967075 31.111109 5 TCTCGGT 95 2.797151E-7 29.473682 44 GGACCGC 60 4.9640134E-4 29.166666 6 CGGACCG 60 4.9640134E-4 29.166666 5 GCTGGGG 50 0.0071908166 27.999998 23 GATTTCG 50 0.0071908166 27.999998 41 TTGAGAA 50 0.0071908166 27.999998 63 GAGCATC 50 0.0071908166 27.999998 40 TGAGAAG 50 0.0071908166 27.999998 64 >>END_MODULE