FastQCFastQC Report
Wed 25 May 2016
SRR1780238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780238_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150722
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT1741411.553721420894096No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC47233.1335836838683138No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG43882.911320178872361No Hit
CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG41742.769336924934648TruSeq Adapter, Index 12 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC10840.7192048937779488No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5260.348986876501108TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT2790.1851090086384204No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2580.17117607250434574No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2560.16984912620586246No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2330.1545892437733045No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1590.10549223072942238TruSeq Adapter, Index 12 (95% over 21bp)
CAGCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGC1570.10416528443093907No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1520.10084791868473084RNA PCR Primer, Index 12 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA150.00222114570.03
AATTACT150.00222114570.028
TCTTTAT1250.061.61
CTTATAT308.3449595E-658.3333321
CTTTATA1450.053.103452
GATACTA200.006940379752.547
GGGCTTA200.006940379752.537
TTACTCG200.006940379752.519
GTCTAAT200.006940379752.559
GTCAGTA200.006940379752.556
AGTCTAA200.006940379752.558
TACTCGC200.006940379752.520
AACTCGT200.006940379752.537
AAGGAGC200.006940379752.56
GAAAGCT305.8630673E-446.66666434
ATAAGAC305.8630673E-446.66666465
TTTATAC1850.045.4054073
AGTCAGT350.001252300740.055
TGAAAGC350.001252300740.033
GCTCGTA350.001252300740.038
Produced by FastQC (version 0.11.5)