##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780238_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 150722 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.71759928875679 34.0 34.0 34.0 34.0 34.0 2 33.7363954830748 34.0 34.0 34.0 34.0 34.0 3 33.748543676437414 34.0 34.0 34.0 34.0 34.0 4 33.76212497180239 34.0 34.0 34.0 34.0 34.0 5 33.756717665636074 34.0 34.0 34.0 34.0 34.0 6 37.52472100954074 38.0 38.0 38.0 37.0 38.0 7 37.50733137829912 38.0 38.0 38.0 37.0 38.0 8 37.441753692228076 38.0 38.0 38.0 37.0 38.0 9 37.47277769668661 38.0 38.0 38.0 37.0 38.0 10-11 37.41467071827603 38.0 38.0 38.0 37.0 38.0 12-13 37.39602712278234 38.0 38.0 38.0 37.0 38.0 14-15 37.31297023659452 38.0 38.0 38.0 36.5 38.0 16-17 37.3310664667401 38.0 38.0 38.0 36.0 38.0 18-19 37.270451559825375 38.0 38.0 38.0 36.0 38.0 20-21 37.31242618861215 38.0 38.0 38.0 36.5 38.0 22-23 37.31302331444647 38.0 38.0 38.0 36.0 38.0 24-25 37.31062485901195 38.0 38.0 38.0 36.0 38.0 26-27 37.22675521821632 38.0 38.0 38.0 36.0 38.0 28-29 37.18406735579411 38.0 38.0 38.0 36.0 38.0 30-31 37.163118191106804 38.0 38.0 38.0 36.0 38.0 32-33 37.08660978490201 38.0 38.0 38.0 36.0 38.0 34-35 37.072066453470626 38.0 38.0 38.0 36.0 38.0 36-37 37.04497684478709 38.0 38.0 38.0 36.0 38.0 38-39 36.38468173193031 38.0 37.0 38.0 34.0 38.0 40-41 36.75883414498215 38.0 37.0 38.0 34.5 38.0 42-43 37.04911028250687 38.0 38.0 38.0 36.0 38.0 44-45 37.128063587266624 38.0 38.0 38.0 36.0 38.0 46-47 37.07428577115484 38.0 38.0 38.0 36.0 38.0 48-49 37.091031833441704 38.0 38.0 38.0 36.0 38.0 50-51 36.906264513475136 38.0 38.0 38.0 35.0 38.0 52-53 36.87755603030745 38.0 38.0 38.0 35.0 38.0 54-55 36.868701981130826 38.0 37.5 38.0 35.5 38.0 56-57 36.91124719682595 38.0 38.0 38.0 35.5 38.0 58-59 36.75482344979498 38.0 37.0 38.0 34.5 38.0 60-61 36.7448813046536 38.0 37.0 38.0 34.5 38.0 62-63 36.74212125635276 38.0 37.0 38.0 34.5 38.0 64-65 36.5954538819814 38.0 37.0 38.0 34.5 38.0 66-67 36.12664375472725 38.0 37.0 38.0 33.0 38.0 68-69 35.95139395708655 38.0 37.0 38.0 32.0 38.0 70-71 34.830983532596434 38.0 36.0 38.0 26.5 38.0 72-73 32.92379679144385 38.0 32.5 38.0 16.0 38.0 74-75 32.354649619829885 38.0 31.0 38.0 11.0 38.0 76 31.777650243494644 37.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.01125590534916654 1101 2 -0.014574955333131356 1101 3 0.02301359842228834 1101 4 0.09718452851355153 1101 5 -0.09615432854577222 1101 6 -0.08655004297391145 1101 7 0.1315195757189116 1101 8 0.051446666792479334 1101 9 0.15392267138665972 1101 10-11 0.2166801308425903 1101 12-13 0.16741930272886663 1101 14-15 0.2944167709496526 1101 16-17 0.12720360963632515 1101 18-19 0.1358487739566101 1101 20-21 0.1896910740375617 1101 22-23 0.17775480015878742 1101 24-25 0.1693042713341697 1101 26-27 0.06968737583274276 1101 28-29 0.1749457856305341 1101 30-31 0.18271184945662355 1101 32-33 -0.028016774001500266 1101 34-35 0.10958373373775743 1101 36-37 0.19709615152889626 1101 38-39 0.13700531538680139 1101 40-41 0.25723761603838113 1101 42-43 0.32273870072307176 1101 44-45 0.11991041745461217 1101 46-47 0.1807748066326269 1101 48-49 -0.12193053087551675 1101 50-51 0.18728467286441486 1101 52-53 0.0994496522187589 1101 54-55 0.31608357880865157 1101 56-57 0.10601575452891865 1101 58-59 0.08900190158760068 1101 60-61 -0.19476152945266278 1101 62-63 -0.019148600597901577 1101 64-65 0.01654761187319309 1101 66-67 -0.07596605614391905 1101 68-69 -0.30060099651090866 1101 70-71 -0.3768646907987616 1101 72-73 -0.6306477719035826 1101 74-75 -0.928109382396272 1101 76 -0.867000251576723 1102 1 -0.027591114067803346 1102 2 0.024860853532729266 1102 3 0.00643590438345143 1102 4 0.030141036599594884 1102 5 -0.027305215655211157 1102 6 0.021276931433376944 1102 7 0.08479586189675814 1102 8 -0.11807235764882051 1102 9 0.06738370660935544 1102 10-11 -0.06449954152879656 1102 12-13 -0.09826866503163245 1102 14-15 0.07449736968310106 1102 16-17 -0.03366020990625174 1102 18-19 0.14328714732762649 1102 20-21 0.09061743610381967 1102 22-23 0.15512565900632325 1102 24-25 -0.1615234357888795 1102 26-27 0.08670993301073793 1102 28-29 -0.022279053433678087 1102 30-31 0.07552600133813314 1102 32-33 0.02247519229357664 1102 34-35 0.08832843463272155 1102 36-37 0.17974648910151103 1102 38-39 -0.06483170769374169 1102 40-41 0.02111342071729183 1102 42-43 0.1856619749157815 1102 44-45 0.1733986678340571 1102 46-47 0.10621443608779657 1102 48-49 -0.03299061356771915 1102 50-51 0.032825574104805355 1102 52-53 0.22833566258145055 1102 54-55 0.1434439702882102 1102 56-57 0.12345046432113804 1102 58-59 0.13834899950983015 1102 60-61 0.07229373825028063 1102 62-63 0.23186542567519552 1102 64-65 0.14194668027744228 1102 66-67 0.22132682081217325 1102 68-69 0.21564305017355423 1102 70-71 -0.09751680782393635 1102 72-73 -0.5544322487968714 1102 74-75 -0.40117553616190804 1102 76 0.4104111683217404 1103 1 -0.17268191848503278 1103 2 0.034647842594331735 1103 3 -0.04175324095595556 1103 4 -0.16447350257453053 1103 5 0.05928608596200036 1103 6 -0.08914071654903921 1103 7 -0.08868767816709777 1103 8 -0.06513364408834832 1103 9 0.019207645479923485 1103 10-11 -0.18228359972735575 1103 12-13 -0.14087085271154365 1103 14-15 -0.05143815133013874 1103 16-17 -0.08264094994916604 1103 18-19 -0.31233775454079904 1103 20-21 -0.016267475185237856 1103 22-23 0.07930920430386124 1103 24-25 -0.12085116908034621 1103 26-27 -0.16347324592891255 1103 28-29 0.024686718273024155 1103 30-31 -0.018065352615913355 1103 32-33 0.10928892548036373 1103 34-35 0.127718448763666 1103 36-37 -0.01922509199442146 1103 38-39 0.2250882169412023 1103 40-41 0.24557958495029908 1103 42-43 -0.09565508166034675 1103 44-45 -0.04071134420341593 1103 46-47 -0.2777744161653004 1103 48-49 -0.03514296610867973 1103 50-51 -0.2906946017469849 1103 52-53 -0.12982495918020476 1103 54-55 -0.3056780792224387 1103 56-57 -0.37844020402030054 1103 58-59 0.0151677046964096 1103 60-61 -0.1766268144267542 1103 62-63 0.1039083942207526 1103 64-65 0.2510035704224123 1103 66-67 -0.029333931791583723 1103 68-69 -0.027284934334737443 1103 70-71 -0.18904085660187064 1103 72-73 0.42634704674927093 1103 74-75 0.570595280816157 1103 76 0.5837769504958139 1104 1 -0.04021737669406633 1104 2 -0.13423005555817724 1104 3 0.09481974276729233 1104 4 0.11347436993293059 1104 5 0.056028117678124545 1104 6 0.19551935567579903 1104 7 0.07216959927049515 1104 8 -0.1332238728003361 1104 9 -0.05887891965327441 1104 10-11 -0.016519743548997212 1104 12-13 0.1062767895138208 1104 14-15 0.09091151109724649 1104 16-17 -0.0020945533405907213 1104 18-19 -0.042318913278435843 1104 20-21 0.08809218357857418 1104 22-23 0.1437620226426901 1104 24-25 -0.02894767821312172 1104 26-27 0.1511038724046827 1104 28-29 0.0635795324249031 1104 30-31 -0.03684773603560387 1104 32-33 0.055303475121853296 1104 34-35 -0.054348333044046626 1104 36-37 -0.08793027857525715 1104 38-39 -0.12291251577455142 1104 40-41 0.0463659459698178 1104 42-43 -0.022671358417554188 1104 44-45 -0.07533870590331304 1104 46-47 0.062022516895879676 1104 48-49 -0.04561687619398924 1104 50-51 -0.2550532137739907 1104 52-53 -0.06080575155996115 1104 54-55 -0.15074794890370669 1104 56-57 -0.41569094982028076 1104 58-59 0.09163182779265355 1104 60-61 -0.12341333245679209 1104 62-63 0.04247148628125785 1104 64-65 -0.16865938032862005 1104 66-67 -0.40872368423833194 1104 68-69 -0.33612462659412046 1104 70-71 -0.45484004014716817 1104 72-73 -0.2539271982918194 1104 74-75 -0.07794321292958983 1104 76 -0.09716458925401383 1105 1 -0.10648304848737666 1105 2 0.028490110930832202 1105 3 0.005230381821142771 1105 4 -0.006481469872156254 1105 5 0.009165014282352502 1105 6 -0.20504406750068682 1105 7 -0.2599455217893549 1105 8 -0.17004941465100387 1105 9 -0.03332184133237348 1105 10-11 0.03548269287555428 1105 12-13 0.023311457804773283 1105 14-15 -0.03800960965804734 1105 16-17 -0.07918454877229664 1105 18-19 0.030659615730250778 1105 20-21 0.08094787618300359 1105 22-23 0.0070176949435918345 1105 24-25 -0.09428700109013732 1105 26-27 0.12795149435280706 1105 28-29 -0.029378946840104447 1105 30-31 0.20624271622570234 1105 32-33 -0.06974093886727672 1105 34-35 0.06360253239424907 1105 36-37 -0.09145801828390177 1105 38-39 -0.22357999458273525 1105 40-41 -0.004335795058558745 1105 42-43 -0.19745944024081297 1105 44-45 -0.3192412996802858 1105 46-47 -0.08186611819878209 1105 48-49 -0.022020358250735228 1105 50-51 -0.4154765425390252 1105 52-53 -0.16173971430297485 1105 54-55 -0.3704449186006755 1105 56-57 -0.020152652271924865 1105 58-59 0.0961620546846973 1105 60-61 0.11982305148310957 1105 62-63 0.0813083473542946 1105 64-65 -0.21589048534866606 1105 66-67 -0.2840726512958369 1105 68-69 0.01628134264601755 1105 70-71 -0.04710058529715866 1105 72-73 0.0822105612127757 1105 74-75 0.31786791340918086 1105 76 0.6280397150960191 1106 1 0.07140260142465138 1106 2 0.1438085165609948 1106 3 -0.020616737908483174 1106 4 0.12387433940090631 1106 5 -0.011543060094567181 1106 6 0.03755431483921967 1106 7 0.02030332952008962 1106 8 -0.02070065574311286 1106 9 0.04587135386274355 1106 10-11 0.14493739712728626 1106 12-13 0.07350575422389483 1106 14-15 0.09115004250706704 1106 16-17 0.03608955474275888 1106 18-19 0.055345387156641834 1106 20-21 -0.08929249443583132 1106 22-23 0.021691188355227098 1106 24-25 0.22356485263694026 1106 26-27 -0.008486362437437833 1106 28-29 0.07680689420390507 1106 30-31 0.05603639494676571 1106 32-33 0.08897138851269659 1106 34-35 0.12568245340991524 1106 36-37 0.08851299547507097 1106 38-39 0.2516899318757595 1106 40-41 0.10218547628556962 1106 42-43 0.05785048469873999 1106 44-45 0.07696200905483153 1106 46-47 0.21659723129427277 1106 48-49 0.21621110131408727 1106 50-51 0.4120141856611639 1106 52-53 0.36152206056633673 1106 54-55 0.34114198316255084 1106 56-57 0.08169474592813941 1106 58-59 -0.03267166049067782 1106 60-61 0.26173538392617957 1106 62-63 0.1551378221843649 1106 64-65 0.40968069549256825 1106 66-67 0.4119452214431689 1106 68-69 0.6270461237302811 1106 70-71 0.3918605933922592 1106 72-73 0.2785448776311483 1106 74-75 0.10408805694803647 1106 76 -0.139190643244806 1107 1 0.0467832831709174 1107 2 0.04859061344339466 1107 3 -0.0475334372743319 1107 4 -0.00899403268623189 1107 5 -0.00842292541613432 1107 6 -0.06685311272681815 1107 7 -0.0036641147541729424 1107 8 0.12894556022336445 1107 9 0.02949222901938242 1107 10-11 -0.010999719184745516 1107 12-13 0.11250743770427363 1107 14-15 0.10646275215100331 1107 16-17 0.14066469745885968 1107 18-19 -0.002003700852668544 1107 20-21 0.16260616764029834 1107 22-23 -0.0017762749056586813 1107 24-25 0.1733009386083637 1107 26-27 0.10031330339803901 1107 28-29 0.13393763607447084 1107 30-31 0.050463821753339744 1107 32-33 0.24181685007744136 1107 34-35 0.07490906505757522 1107 36-37 0.08316509729399257 1107 38-39 -0.15322823578876665 1107 40-41 0.241895360720342 1107 42-43 0.18570638890491153 1107 44-45 0.031261212882533584 1107 46-47 0.034968052655486304 1107 48-49 0.11240175230202709 1107 50-51 -0.03733583902143778 1107 52-53 0.15107435604878816 1107 54-55 0.14086161406263642 1107 56-57 -0.19603204925684992 1107 58-59 0.29716707981032187 1107 60-61 -0.09123222489880334 1107 62-63 0.1830671413241447 1107 64-65 0.40973765535485285 1107 66-67 0.5224600120207441 1107 68-69 0.25874365420380485 1107 70-71 -0.221723700875053 1107 72-73 -0.2921612980837054 1107 74-75 -0.45851399613856003 1107 76 -0.010151239677853141 1108 1 -0.21193454841123582 1108 2 -0.14433106565918763 1108 3 -0.08194793400038947 1108 4 -0.10117209471354016 1108 5 0.013098824748830395 1108 6 -0.0014503412938964289 1108 7 0.04186656896746399 1108 8 0.15212966255320026 1108 9 -0.005848616622969871 1108 10-11 -0.024095501124755003 1108 12-13 -0.028824220587189586 1108 14-15 0.09469938988512183 1108 16-17 -0.12835717960322057 1108 18-19 0.13192351096398625 1108 20-21 -0.03059468510829788 1108 22-23 -0.02795514907447938 1108 24-25 0.18064828138283673 1108 26-27 0.05514427644509112 1108 28-29 0.05726640111177517 1108 30-31 -0.3714436956315623 1108 32-33 -0.17828238346400127 1108 34-35 -0.15914631284202585 1108 36-37 0.08631214566717205 1108 38-39 -0.33755898042102217 1108 40-41 -0.2137350641311926 1108 42-43 -0.028984489730682128 1108 44-45 0.13551987995526815 1108 46-47 -0.012472432599068384 1108 48-49 -0.04687950245661199 1108 50-51 -0.32702291074368617 1108 52-53 -0.2262097919640027 1108 54-55 -0.29342471658047486 1108 56-57 -0.3613980205273464 1108 58-59 -0.3970045358437062 1108 60-61 -0.0792214132648752 1108 62-63 -0.44742750361773176 1108 64-65 0.24295678128754616 1108 66-67 0.10769045717581349 1108 68-69 -0.031831697301193174 1108 70-71 -0.1669612522683792 1108 72-73 -0.337260531625148 1108 74-75 -0.46304422303059667 1108 76 -0.5087807508701765 1109 1 0.028524909005824384 1109 2 -0.019749841950783775 1109 3 -0.07261520521628739 1109 4 -0.08192727746373407 1109 5 -0.016612251096908892 1109 6 -0.18493103350277096 1109 7 -0.022480140017648864 1109 8 -0.07746680083378976 1109 9 -0.0790053712011698 1109 10-11 -0.055192002296067244 1109 12-13 0.012634632280644098 1109 14-15 -0.10865585835790625 1109 16-17 0.006123537795197365 1109 18-19 9.81925533629635E-4 1109 20-21 0.22871915151227284 1109 22-23 0.02702734418731012 1109 24-25 0.06266672013444463 1109 26-27 -0.18252858685121964 1109 28-29 -0.10411045976633204 1109 30-31 -0.008481152494454136 1109 32-33 -0.14836034512550356 1109 34-35 0.020118278205607965 1109 36-37 -0.13097292448573228 1109 38-39 0.08553102184604455 1109 40-41 -0.03766954610807005 1109 42-43 -0.08791859386429479 1109 44-45 -0.18178030392845557 1109 46-47 0.030428522084932297 1109 48-49 -0.09990980548691653 1109 50-51 -0.2572258125800033 1109 52-53 -0.02010573392727366 1109 54-55 -0.23827534254082394 1109 56-57 0.1150088159169016 1109 58-59 0.06359585711329885 1109 60-61 0.023769216222007117 1109 62-63 -0.2339806913554625 1109 64-65 -0.2222627456526851 1109 66-67 -0.36501351521643954 1109 68-69 -0.5795312615219572 1109 70-71 0.036932422635267415 1109 72-73 -0.4734856254562274 1109 74-75 -0.2940727124132039 1109 76 -0.17801675177700105 1110 1 0.10041393643724916 1110 2 -0.06291692424505158 1110 3 0.026536914484459828 1110 4 -0.005237751279196345 1110 5 -0.04222945807945422 1110 6 -0.27660185644541 1110 7 -0.058739491638597485 1110 8 0.03599329891683567 1110 9 0.03197966620531645 1110 10-11 -0.10428489506414707 1110 12-13 -0.047574220587186744 1110 14-15 -0.1468915192057878 1110 16-17 0.005746355750318344 1110 18-19 0.09665835944883838 1110 20-21 0.1276250118613973 1110 22-23 -0.10710918947852122 1110 24-25 0.062277069261625684 1110 26-27 0.03253064008145401 1110 28-29 0.16234215868752955 1110 30-31 -0.011847736035605294 1110 32-33 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0.14627111261330583 2115 52-53 0.16630795477288274 2115 54-55 0.1446684315252682 2115 56-57 0.20637988446718936 2115 58-59 0.08193791129772876 2115 60-61 0.011105661004265244 2115 62-63 -0.004811799981879972 2115 64-65 0.18298044360339816 2115 66-67 0.5756731811409068 2115 68-69 0.2643257207076104 2115 70-71 0.039215757120210526 2115 72-73 0.15008140156061245 2115 74-75 0.028521306318310735 2115 76 -0.29536192787956494 2116 1 -0.0835217071271046 2116 2 0.05401376882425524 2116 3 0.021487409533968105 2116 4 0.11354937743367799 2116 5 -0.10388787392103893 2116 6 -0.051255321791948916 2116 7 -0.13012563025245782 2116 8 0.11275072465940639 2116 9 -0.21559459122043023 2116 10-11 -0.029421033678005415 2116 12-13 -0.1463984780129337 2116 14-15 -0.06562914296816302 2116 16-17 -0.5769145353387941 2116 18-19 -0.2856807494620526 2116 20-21 -0.1442685524950349 2116 22-23 -0.10672552611217867 2116 24-25 -0.20350263370866628 2116 26-27 0.0017014321606581007 2116 28-29 -0.06015784131246704 2116 30-31 -0.09996654791679305 2116 32-33 -0.21714877010266775 2116 34-35 -0.10192809101984324 2116 36-37 -0.39514850039743976 2116 38-39 -0.33519624414739013 2116 40-41 -0.586597350359817 2116 42-43 -0.025796670948814437 2116 44-45 -0.029034075440272034 2116 46-47 0.12991680632482883 2116 48-49 -0.18530584509088044 2116 50-51 -0.05924613306591198 2116 52-53 -0.10582275326013502 2116 54-55 -0.15262313642245573 2116 56-57 -0.1367380545307455 2116 58-59 0.0042855931691931914 2116 60-61 -0.21318480960451325 2116 62-63 -0.1151317740447837 2116 64-65 -0.22482749714029637 2116 66-67 -0.33336614848475676 2116 68-69 -0.4511486289943676 2116 70-71 0.01575701955880504 2116 72-73 -0.09889919549153348 2116 74-75 -0.1382617447827741 2116 76 -0.5042803008251724 2117 1 -0.016426165085590583 2117 2 0.15317444631332933 2117 3 0.14724757438293778 2117 4 -0.10037734518782315 2117 5 0.08560049115912705 2117 6 -0.10543434375505711 2117 7 0.06501685353906339 2117 8 -0.6391716757024568 2117 9 -0.0999238870941781 2117 10-11 0.03501079021504694 2117 12-13 0.03906918042803653 2117 14-15 0.040938430032795736 2117 16-17 -0.030820903132934063 2117 18-19 0.10604287721533723 2117 20-21 -0.19910722164114247 2117 22-23 0.08900121661284999 2117 24-25 -0.29416328606832565 2117 26-27 0.14403444718804792 2117 28-29 -0.5163761154241442 2117 30-31 0.04627409137556526 2117 32-33 -0.17244353815815572 2117 34-35 0.10442211252843236 2117 36-37 0.037460686917235364 2117 38-39 0.10948762779310073 2117 40-41 0.041744862034015284 2117 42-43 0.07140391205365404 2117 44-45 -0.04215791320475404 2117 46-47 -0.03755190755163795 2117 48-49 -0.02757478010620673 2117 50-51 -0.057468226848897075 2117 52-53 -0.08194352523580761 2117 54-55 -0.010038827230111735 2117 56-57 -0.10966110825743414 2117 58-59 -0.06649769074294909 2117 60-61 0.1055001680303107 2117 62-63 0.10349414946127666 2117 64-65 -0.22627872094288648 2117 66-67 -0.02068081903810537 2117 68-69 0.04773939432573826 2117 70-71 0.18023533287236404 2117 72-73 0.16032958271622988 2117 74-75 0.07171965944902325 2117 76 0.08651228097107833 2118 1 0.04842414051888255 2118 2 -0.0922026385307646 2118 3 -0.016626350821660196 2118 4 0.015221432394277201 2118 5 0.09251543987973321 2118 6 0.10329610273826262 2118 7 0.09095438591242555 2118 8 0.13543207442703675 2118 9 0.17238782947062248 2118 10-11 0.00926102330320333 2118 12-13 0.11530843247403055 2118 14-15 0.20984317467176083 2118 16-17 0.14679227411766504 2118 18-19 0.008469583938627068 2118 20-21 0.06305868533078751 2118 22-23 -0.10407347519280563 2118 24-25 -0.003411706248577673 2118 26-27 0.05526023191818297 2118 28-29 0.14137277093242773 2118 30-31 0.09963185580112821 2118 32-33 -0.004555316879795157 2118 34-35 -0.08595264143406922 2118 36-37 0.049335233690257496 2118 38-39 -0.04349590064364861 2118 40-41 0.1097807058131508 2118 42-43 0.124347297529674 2118 44-45 0.06279003043970732 2118 46-47 0.1590514799962719 2118 48-49 0.2278963169620596 2118 50-51 0.023510484432563317 2118 52-53 0.15052954013247444 2118 54-55 0.14103467323605656 2118 56-57 0.12551189495399484 2118 58-59 0.05437146910372803 2118 60-61 0.14159645933871445 2118 62-63 0.25952724797987514 2118 64-65 -0.06764927931851616 2118 66-67 -0.015157924601140849 2118 68-69 -0.11677552331644847 2118 70-71 0.020770658677811582 2118 72-73 -0.28088040381645385 2118 74-75 -0.06408009192360709 2118 76 -0.33763047257704315 2119 1 0.12524554265486643 2119 2 0.06573592549588625 2119 3 0.12404986785233518 2119 4 -0.047893191693702875 2119 5 -0.09874500212090709 2119 6 0.02225824718153291 2119 7 -0.01614881806347057 2119 8 -0.15321654564274922 2119 9 -0.0947819918257764 2119 10-11 -0.0061177966185468335 2119 12-13 -0.024290541830715995 2119 14-15 -1.0643915192057847 2119 16-17 -0.1746098618662657 2119 18-19 -0.018296303763598587 2119 20-21 0.027773975591969702 2119 22-23 -0.042452453727229056 2119 24-25 -0.14157655768137545 2119 26-27 0.012692557179896369 2119 28-29 0.14126702915385891 2119 30-31 6.93624099952217E-5 2119 32-33 -0.35314630768025523 2119 34-35 -0.19741108491508896 2119 36-37 0.009272317332005287 2119 38-39 -0.42387551362874376 2119 40-41 -0.25053440468700927 2119 42-43 -0.13840119564998332 2119 44-45 0.07457362988984784 2119 46-47 -0.10160861305648439 2119 48-49 -0.13099788838847104 2119 50-51 -0.11064278827548435 2119 52-53 -0.21013584000922236 2119 54-55 -0.4067143486524927 2119 56-57 0.0036841483113008167 2119 58-59 -0.10270794297198194 2119 60-61 -0.0561604931832278 2119 62-63 0.2321467361896623 2119 64-65 -0.2593591238780988 2119 66-67 -0.2003183877501371 2119 68-69 -0.38868389806531667 2119 70-71 -0.2732377477935799 2119 72-73 -0.38841979262897297 2119 74-75 -0.5084202632252897 2119 76 -1.5405753811104042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 0.0 21 0.0 22 7.0 23 8.0 24 27.0 25 67.0 26 129.0 27 297.0 28 518.0 29 840.0 30 1349.0 31 2181.0 32 3510.0 33 5960.0 34 9875.0 35 16883.0 36 24400.0 37 84669.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.04442616207322 14.84388476798344 13.045872533538569 40.06581653640477 2 17.065856344793726 18.122105598386433 40.01340215761468 24.79863589920516 3 16.813073074932657 16.491952070699696 36.66485317339207 30.03012168097557 4 13.617786388184872 27.80151537267287 31.58862010854421 26.992078130598056 5 14.17709425299558 28.35418850599116 42.91211634665145 14.556600894361804 6 25.88938575655843 40.60920104563368 16.304852642613554 17.19656055519433 7 24.568410716418306 23.270657236501638 22.51562479266464 29.645307254415414 8 20.68244848130996 40.11292313000093 14.927482384787888 24.277146003901223 9 19.79273098817691 15.880229827098896 15.301681240960177 49.025357943764014 10-11 24.36472445960112 28.58905800082271 23.385106354745826 23.66111118483035 12-13 23.325725507888695 17.511046827934873 27.261116492615546 31.902111171560886 14-15 20.41606401188944 16.108464590438025 19.68956091346983 43.7859104842027 16-17 28.901553854115527 25.26970183516673 16.74506707713539 29.083677233582357 18-19 32.242141160547234 22.907405687291835 17.251297090006766 27.59915606215416 20-21 24.355767572086357 19.365122543490664 16.94643117793023 39.33267870649274 22-23 21.254030599381643 25.6674539881371 18.439245763723942 34.63926964875731 24-25 29.01533949921047 27.55138599540877 17.011783283130534 26.42149122225024 26-27 30.277928902217326 27.99591300540067 20.669179018325128 21.056979074056873 28-29 24.14909568609758 22.91404041878425 24.440692135189288 28.496171759928874 30-31 20.56501373389419 27.90335850108146 22.08569419195605 29.4459335730683 32-33 19.923435198577515 20.495017316649193 33.416488634704955 26.16505885006834 34-35 28.07851541248126 23.808402223962 26.2154828094107 21.897599554146044 36-37 23.915221400989903 35.200567933015755 19.905521423547988 20.978689242446357 38-39 19.39862793752737 25.347328193628005 28.323005267976804 26.931038600867822 40-41 20.801210175024217 16.99619166412335 29.786295298629263 32.41630286222317 42-43 28.04368307214607 16.748052706306975 28.69819933387296 26.510064887673995 44-45 20.142049601252637 24.89915208131527 32.43156274465572 22.52723557277637 46-47 25.5543318161914 19.726051936678125 27.795875850904313 26.923740396226165 48-49 25.732806093337402 17.484176165390586 22.441647536524197 34.34137020474781 50-51 22.22568702644604 18.075330741364898 28.66767956900784 31.031302663181222 52-53 16.36755085521689 18.18944812303446 40.31296028449729 25.130040737251363 54-55 17.525643237218187 19.408911771340616 33.25393771314074 29.811507278300446 56-57 24.120566340680195 20.969400618356975 24.53789095155319 30.372142089409643 58-59 19.023102135056593 21.52704980029458 33.445017980122344 26.004830084526482 60-61 24.339512479929937 27.54043868844628 25.533432411990287 22.586616419633497 62-63 18.124096017834155 39.89663088334815 19.0486458513024 22.930627247515293 64-65 16.31115563753135 42.53128275898674 18.175846923475007 22.981714680006903 66-67 16.743076657687663 41.803784450843274 18.19276548878067 23.26037340268839 68-69 16.411008346492217 42.30537015166996 18.252146335637796 23.031475166200025 70-71 16.159883759504254 40.26154111543106 19.325314154536166 24.253260970528522 72-73 16.558299385623865 36.054126139515134 20.785286819442415 26.602287655418582 74-75 16.808760499462586 33.908785711442256 22.344116983585675 26.938336805509483 76 17.045288677167235 34.70097265163679 21.893286978675974 26.360451692520005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 1.0 14 0.5 15 1.5 16 3.0 17 4.0 18 5.5 19 11.5 20 18.0 21 20.0 22 33.0 23 61.0 24 85.0 25 94.0 26 143.0 27 238.0 28 349.0 29 414.0 30 513.5 31 645.0 32 937.5 33 1198.0 34 1373.5 35 1699.5 36 2152.5 37 2455.0 38 6550.5 39 12980.5 40 14307.0 41 11502.5 42 9706.0 43 8505.0 44 6996.5 45 6320.5 46 5952.0 47 5804.0 48 5512.0 49 4953.5 50 4539.0 51 4131.0 52 3512.5 53 3142.0 54 2982.0 55 2969.5 56 2866.0 57 2833.0 58 2891.0 59 2933.0 60 3075.5 61 3306.0 62 3436.0 63 3354.5 64 3486.0 65 3664.5 66 3599.5 67 3569.0 68 3566.0 69 3299.5 70 2898.0 71 2760.0 72 2392.0 73 1719.5 74 1150.0 75 885.0 76 669.5 77 304.0 78 103.5 79 53.0 80 31.5 81 6.0 82 1.5 83 1.0 84 0.5 85 1.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 150722.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.93665158371041 #Duplication Level Percentage of deduplicated Percentage of total 1 50.6119257086999 17.17599288756784 2 23.6911045943304 16.079935245020636 3 11.511241446725318 11.71958970820451 4 5.1867057673509285 7.040777059752392 5 2.844574780058651 4.826767160733005 6 1.6148582600195502 3.288172927641618 7 1.0694037145650048 2.5404386884462786 8 0.729227761485826 1.979803877337084 9 0.5474095796676441 1.6719523360889585 >10 2.142717497556207 10.537280556255888 >50 0.019550342130987292 0.46907551651384666 >100 0.019550342130987292 1.2340600575894694 >500 0.0019550342130987292 0.348986876501108 >1k 0.007820136852394917 9.533445681453271 >5k 0.0 0.0 >10k+ 0.0019550342130987292 11.553721420894096 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 17414 11.553721420894096 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 4723 3.1335836838683138 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 4388 2.911320178872361 No Hit CACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTTCTG 4174 2.769336924934648 TruSeq Adapter, Index 12 (96% over 26bp) TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 1084 0.7192048937779488 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 526 0.348986876501108 TruSeq Adapter, Index 12 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT 279 0.1851090086384204 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 258 0.17117607250434574 No Hit CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 256 0.16984912620586246 No Hit CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 233 0.1545892437733045 No Hit CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC 159 0.10549223072942238 TruSeq Adapter, Index 12 (95% over 21bp) CAGCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGC 157 0.10416528443093907 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT 152 0.10084791868473084 RNA PCR Primer, Index 12 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012605989835591353 0.0 2 0.0 0.0 0.0 0.014596409283316304 0.0 3 0.0 0.0 0.0 0.014596409283316304 0.0 4 0.0 0.0 0.0 0.03582755005904911 0.0 5 0.0 0.0 0.0 0.042462281551465614 0.0 6 0.0 0.0 0.0 0.043125754700707265 0.0 7 0.0 0.0 0.0 0.043125754700707265 0.0 8 0.0 0.0 0.0 0.043125754700707265 0.0 9 0.0 0.0 0.0 0.05639521768554027 0.0 10 0.0 0.0 0.0 0.06568384177492337 0.0 11 0.0 0.0 0.0 0.06568384177492337 0.0 12 0.0 0.0 0.0 0.06634731492416501 0.0 13 0.0 0.0 0.0 0.06833773437188997 0.0 14 0.0 0.0 0.0 0.07032815381961492 0.0 15 0.0 0.0 0.0 0.07165510011809822 0.0 16 0.0 0.0 0.0 0.07231857326733987 0.0 17 0.0 0.0 0.0 0.07298204641658151 0.0 18 0.0 0.0 0.0 0.07961677790899803 0.0 19 0.0 0.0 0.0 0.08293414365520628 0.0 20 0.0 0.0 0.0 0.08757845569989782 0.0 21 0.0 0.0 0.0 0.09023234829686443 0.0 22 0.0 0.0 0.0 0.09222276774458937 0.0 23 0.0 0.0 0.0 0.10084791868473082 0.0 24 0.0 0.0 0.0 0.10681917702790568 0.0 25 0.0 0.0 0.0 0.11942516686349704 0.0 26 0.0 0.0 0.0 0.13136768354984674 0.0 27 0.0 0.0 0.0 0.24681201151789386 0.0 28 0.0 0.0 0.0 0.3821605339631905 0.0 29 0.0 0.0 0.0 0.5228168416024204 0.0 30 0.0 0.0 0.0 0.6455593742121256 0.0 31 0.0 0.0 0.0 0.7855522087021138 0.0 32 0.0 0.0 0.0 0.9447857645201099 0.0 33 0.0 0.0 0.0 1.2088480779182866 0.0 34 0.0 0.0 0.0 1.5890181924337523 0.0 35 0.0 0.0 0.0 2.0667188598877404 0.0 36 0.0 0.0 0.0 2.687066254428683 0.0 37 0.0 0.0 0.0 3.5482544021443454 0.0 38 0.0 0.0 0.0 4.397500033173658 0.0 39 0.0 0.0 0.0 5.237457040113586 0.0 40 0.0 0.0 0.0 6.0920104563368325 0.0 41 0.0 0.0 0.0 6.913390215097995 0.0 42 0.0 0.0 0.0 7.7984633961863565 0.0 43 0.0 0.0 0.0 8.67623837263306 0.0 44 0.0 0.0 0.0 9.599792996377436 0.0 45 0.0 0.0 0.0 10.547896126643755 0.0 46 0.0 0.0 0.0 11.465479492044956 0.0 47 0.0 0.0 0.0 12.265628110030388 0.0 48 0.0 0.0 0.0 12.996112047345443 0.0 49 0.0 0.0 0.0 13.77038521251045 0.0 50 0.0 0.0 0.0 14.451772136781624 0.0 51 0.0 0.0 0.0 15.094677618396783 0.0 52 0.0 0.0 0.0 15.819853770517907 0.0 53 0.0 0.0 0.0 16.525125728161782 0.0 54 0.0 0.0 0.0 17.203858759835988 0.0 55 0.0 0.0 0.0 17.908467244330623 0.0 56 0.0 0.0 0.0 18.55535356484123 0.0 57 0.0 0.0 0.0 19.19892251960563 0.0 58 0.0 0.0 0.0 19.80467350486326 0.0 59 0.0 0.0 0.0 20.44426162073221 0.0 60 0.0 0.0 0.0 21.01949284112472 0.0 61 0.0 0.0 0.0 21.611974363397515 0.0 62 0.0 0.0 0.0 22.210427144013483 0.0 63 0.0 0.0 0.0 22.806226032032484 0.0 64 0.0 0.0 0.0 23.38411114502196 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTATA 15 0.002221145 70.0 3 AATTACT 15 0.002221145 70.0 28 TCTTTAT 125 0.0 61.6 1 CTTATAT 30 8.3449595E-6 58.333332 1 CTTTATA 145 0.0 53.10345 2 GATACTA 20 0.0069403797 52.5 47 GGGCTTA 20 0.0069403797 52.5 37 TTACTCG 20 0.0069403797 52.5 19 GTCTAAT 20 0.0069403797 52.5 59 GTCAGTA 20 0.0069403797 52.5 56 AGTCTAA 20 0.0069403797 52.5 58 TACTCGC 20 0.0069403797 52.5 20 AACTCGT 20 0.0069403797 52.5 37 AAGGAGC 20 0.0069403797 52.5 6 GAAAGCT 30 5.8630673E-4 46.666664 34 ATAAGAC 30 5.8630673E-4 46.666664 65 TTTATAC 185 0.0 45.405407 3 AGTCAGT 35 0.0012523007 40.0 55 TGAAAGC 35 0.0012523007 40.0 33 GCTCGTA 35 0.0012523007 40.0 38 >>END_MODULE