FastQCFastQC Report
Wed 25 May 2016
SRR1780237_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780237_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349909
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAA301.2922502E-436.70030615
CTTACCG353.2283217E-431.4034619
TCAGATG502.3698522E-630.7753934
GACTTAA300.005713831629.36024515
GGCTATA300.0057378629.3350530
AGTTTAG300.00575794529.31408744
ATATACG300.005761968429.3098961
GCTTACC454.083586E-529.3098968
TACCGAA407.0372963E-427.48981711
GACGAAA959.567884E-1025.47517423
TGCTTAC450.001402747624.4249157
AAAGTTG500.002563117322.02018415
AATTCTA703.2054835E-522.00128723
TGTATAA755.473986E-520.52572611
CAGATGT755.4920936E-520.516935
CTTTACA755.4920936E-520.516931
AACTAGA550.00447583320.00117127
TTACCGA550.004486961319.99259210
CTGTTAC550.00449811219.9840227
CTAATGC550.00449811219.9840223
Produced by FastQC (version 0.11.5)