FastQCFastQC Report
Wed 25 May 2016
SRR1780237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780237_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349909
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGT185245.293947855013732No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC53161.519252148415731No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCG47071.345206896650272No Hit
CACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTTCTG29980.8567941950621447Illumina PCR Primer Index 2 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCC9460.27035600684749467No Hit
ATACACATCTCCGAGCCCACGAGACCCGTTTGTATCTCGTATGCCGTCTT4410.12603276851981515Illumina PCR Primer Index 2 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTCTG3900.037.79401444
TCTTTAT1850.036.9009361
GTCTTCT4100.035.950443
CGTCTTC4250.035.71683542
TTGTACT250.002350778335.19924
TGTACTA250.002350778335.19925
CTTTATA1950.034.9735642
ATGCCGT25300.034.17313844
CCGTCTT4400.033.49923741
TTTATAC2300.029.6514993
CAGTACG300.00574014529.3326664
ATACAGC300.00574014529.3326666
TATGCCG30450.028.39344443
GCCGTCT5550.026.95434440
TTAATAC551.5907102E-423.9994542
TGCCGTC6550.022.50330539
TTGTATC37950.022.4922433
TGTATCT37950.022.4342734
GTATCTC38000.022.40475535
CGTATGC38600.022.39845541
Produced by FastQC (version 0.11.5)