FastQCFastQC Report
Wed 25 May 2016
SRR1780236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206478
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTA200.00694400152.50000460
GTAAGCG200.00694400152.50000457
TCAACGT200.00694400152.5000042
CCATTAG200.00694400152.50000432
CACAAGG305.8681413E-446.66666415
GACGAAT350.001253377940.023
CTTAGGT400.00241485435.0000044
CCACAAG552.985016E-431.8181814
CTGACAT552.985016E-431.8181821
CGTATCC450.004300357331.11111356
GTCTACT450.004300357331.1111131
TCTCGTT500.007196889327.99999840
GGAAAGA500.007196889327.99999851
GCCGTAT500.007196889327.99999854
CTGTGTT500.007196889327.9999989
GAAAAGA657.937119E-426.92307734
GAAAAAG808.636905E-526.25000213
CTTGGTG851.3039443E-424.70588316
CGGGACC851.3039443E-424.7058834
GATCTCT851.3039443E-424.70588341