##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780236_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 206478 Sequences flagged as poor quality 0 Sequence length 76 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.18565658326795 34.0 31.0 34.0 12.0 34.0 2 30.252782378752215 34.0 31.0 34.0 12.0 34.0 3 30.038977518185956 34.0 31.0 34.0 11.0 34.0 4 29.953656079582327 34.0 31.0 34.0 11.0 34.0 5 29.979150321099585 34.0 31.0 34.0 11.0 34.0 6 32.71874485417332 37.0 33.0 38.0 11.0 38.0 7 33.14064936700278 38.0 34.0 38.0 12.0 38.0 8 33.042682513391256 38.0 34.0 38.0 11.0 38.0 9 33.42919826809636 38.0 34.0 38.0 12.0 38.0 10-11 33.1820097056345 38.0 34.0 38.0 11.0 38.0 12-13 33.22701934346516 38.0 34.0 38.0 11.0 38.0 14-15 33.14730140741386 38.0 34.0 38.0 11.0 38.0 16-17 33.19347581824698 38.0 34.0 38.0 11.0 38.0 18-19 33.220638518389364 38.0 34.0 38.0 11.0 38.0 20-21 33.21752438516452 38.0 34.0 38.0 11.0 38.0 22-23 33.2362866746094 38.0 34.0 38.0 11.0 38.0 24-25 33.23421139298134 38.0 34.0 38.0 11.0 38.0 26-27 33.194509826712775 38.0 34.0 38.0 11.0 38.0 28-29 33.26891484807098 38.0 34.0 38.0 11.0 38.0 30-31 33.277276513720594 38.0 34.0 38.0 11.0 38.0 32-33 33.324906285415395 38.0 34.0 38.0 11.0 38.0 34-35 33.27859384534914 38.0 34.0 38.0 11.0 38.0 36-37 33.21141235385852 38.0 34.0 38.0 11.0 38.0 38-39 33.19158215403094 38.0 34.0 38.0 11.0 38.0 40-41 33.24700694504983 38.0 34.0 38.0 11.0 38.0 42-43 33.339363031412546 38.0 34.0 38.0 11.0 38.0 44-45 33.40955937194278 38.0 34.0 38.0 11.0 38.0 46-47 33.335575702980464 38.0 34.0 38.0 11.0 38.0 48-49 33.28346603512239 38.0 34.0 38.0 11.0 38.0 50-51 33.241771520452545 38.0 34.0 38.0 11.0 38.0 52-53 33.300235376165986 38.0 34.0 38.0 11.0 38.0 54-55 33.250564224760026 38.0 34.0 38.0 11.0 38.0 56-57 33.22152481136005 38.0 34.0 38.0 11.0 38.0 58-59 33.256230688015194 38.0 34.0 38.0 11.0 38.0 60-61 33.28066670541172 38.0 34.0 38.0 11.0 38.0 62-63 33.14320411859859 38.0 34.0 38.0 11.0 38.0 64-65 33.08753474946484 38.0 34.0 38.0 11.0 38.0 66-67 33.00398347523707 38.0 33.5 38.0 11.0 38.0 68-69 32.919315859316725 38.0 33.0 38.0 11.0 38.0 70-71 32.87372746733308 38.0 33.0 38.0 11.0 38.0 72-73 32.82402241401021 38.0 33.0 38.0 11.0 38.0 74-75 32.78878621451196 38.0 32.5 38.0 11.0 38.0 76 31.10043200728407 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.708888829855983 1101 2 -0.28215514052061863 1101 3 -0.656994576233771 1101 4 0.3098377354295998 1101 5 -0.8436036416840196 1101 6 -1.1225202933988712 1101 7 -0.24732932067438895 1101 8 -0.5361993436244958 1101 9 -0.6559973696347683 1101 10-11 -0.7294454942961082 1101 12-13 -0.5881505795956983 1101 14-15 -0.5303193577665439 1101 16-17 -0.8065768633697203 1101 18-19 -0.6336819351764973 1101 20-21 -0.4735422909603457 1101 22-23 -0.3940994079277971 1101 24-25 -0.45183843770787746 1101 26-27 -0.6121665057689114 1101 28-29 -0.6074820543475283 1101 30-31 -0.8924287697415139 1101 32-33 -0.7171032543316684 1101 34-35 -0.7007433374434555 1101 36-37 -0.5851177797565086 1101 38-39 -0.7288098523999977 1101 40-41 -0.3971130691529794 1101 42-43 -0.9213161275012425 1101 44-45 -0.36643679337475277 1101 46-47 -0.39417490295192437 1101 48-49 -0.676373004761075 1101 50-51 -0.5537772098176319 1101 52-53 -0.6073312638787485 1101 54-55 -0.36415209620498956 1101 56-57 -0.7243899449021427 1101 58-59 -0.4404559029275461 1101 60-61 -0.37847755249182313 1101 62-63 -0.3971616034292822 1101 64-65 -0.4546719569357407 1101 66-67 -0.4233057635227411 1101 68-69 -0.4399622361098281 1101 70-71 -0.797544796373387 1101 72-73 -0.5800204273919647 1101 74-75 -0.5181213936215272 1101 76 -1.5608603527480867 1102 1 -0.05091681274434734 1102 2 0.08652859071941066 1102 3 0.014001767071935234 1102 4 0.5584223547239553 1102 5 0.026470762867788267 1102 6 0.6598358036800107 1102 7 -0.3648906303074497 1102 8 -0.2519125401965212 1102 9 -0.40049820968616245 1102 10-11 0.05387066151577358 1102 12-13 0.3076901071286713 1102 14-15 0.2818356211262767 1102 16-17 0.24376268681452729 1102 18-19 -0.020158519626363613 1102 20-21 0.07171812497097818 1102 22-23 -0.1386161313038201 1102 24-25 -0.14170417067036567 1102 26-27 0.10703219815179921 1102 28-29 0.048562786042708694 1102 30-31 0.41792962865462613 1102 32-33 0.28414093943077035 1102 34-35 0.3990586504428606 1102 36-37 0.27977513133922827 1102 38-39 0.1276843095957858 1102 40-41 -0.2647290880153079 1102 42-43 0.2726757402365507 1102 44-45 0.4163940386290861 1102 46-47 -0.2887025681737967 1102 48-49 -0.06934410416950954 1102 50-51 0.11219470846442192 1102 52-53 0.17384064310850533 1102 54-55 -0.4083553886417093 1102 56-57 0.11885752435479446 1102 58-59 0.030904697956273708 1102 60-61 0.11496616406495264 1102 62-63 0.07771980108017118 1102 64-65 -0.03262554939081497 1102 66-67 -0.12337741496494203 1102 68-69 0.4319705174233306 1102 70-71 0.0429848595585014 1102 72-73 0.32812454147671843 1102 74-75 -0.19749177863331369 1102 76 0.08598274824161933 1103 1 0.0463820525632066 1103 2 0.07011535219113796 1103 3 0.5330324887687148 1103 4 0.013635058961849467 1103 5 0.2859186628097703 1103 6 -0.22856361885463627 1103 7 0.24678752720027575 1103 8 -0.15542657881114508 1103 9 0.48957087028199453 1103 10-11 0.13154514915019178 1103 12-13 0.4629851136723815 1103 14-15 0.08869335956685376 1103 16-17 0.3010235041708711 1103 18-19 0.09552681572179722 1103 20-21 0.46684677291948873 1103 22-23 0.21532257371661956 1103 24-25 0.26111164756297 1103 26-27 0.10212610373351083 1103 28-29 0.3369041921978422 1103 30-31 0.031447782384546485 1103 32-33 0.3232261020790119 1103 34-35 0.2608329397422082 1103 36-37 0.5570664410252633 1103 38-39 -0.3084049141990235 1103 40-41 0.7263508936353276 1103 42-43 0.38172966848512857 1103 44-45 0.3238494807004102 1103 46-47 0.3579372040781763 1103 48-49 0.47204653495511195 1103 50-51 0.11141047542746918 1103 52-53 0.7870383575329924 1103 54-55 0.4641013351868395 1103 56-57 0.7459690507252645 1103 58-59 0.516602985798464 1103 60-61 0.3163690136136381 1103 62-63 0.2497670372068299 1103 64-65 0.6013029015718558 1103 66-67 0.4318106660421108 1103 68-69 0.6524985339377523 1103 70-71 0.43202684722664486 1103 72-73 0.36520780172740075 1103 74-75 0.650021275052076 1103 76 0.6387270579141635 1104 1 -0.07965768865675571 1104 2 -0.19433229378037353 1104 3 -0.34466879969491515 1104 4 -0.16424563582992846 1104 5 -0.7003030671021548 1104 6 -0.5619331958906244 1104 7 -0.3817836101271155 1104 8 -0.581010328947059 1104 9 -0.5094505497462762 1104 10-11 -0.5544309164146668 1104 12-13 -0.2838124154131876 1104 14-15 -0.30835866826831193 1104 16-17 -0.7076783823138513 1104 18-19 -0.5429150675009922 1104 20-21 -0.8166593975708807 1104 22-23 -0.6009524344382129 1104 24-25 -0.5728508537961758 1104 26-27 -0.3312862861762369 1104 28-29 -0.6634984526863619 1104 30-31 -0.17118452978246523 1104 32-33 -0.3346667570054649 1104 34-35 -0.5500275990204244 1104 36-37 -0.43397003826145664 1104 38-39 -0.3903558900607109 1104 40-41 -0.604225653064681 1104 42-43 -0.35272008415159917 1104 44-45 -0.3852778162442476 1104 46-47 -0.4721772354129641 1104 48-49 -0.5145727430703317 1104 50-51 -0.22569843236855291 1104 52-53 -0.25986212632044214 1104 54-55 -0.2527628596593132 1104 56-57 -0.20137111454602064 1104 58-59 -0.33489070779727115 1104 60-61 -0.26388473760958675 1104 62-63 -0.5042628094823058 1104 64-65 -0.530834276312234 1104 66-67 -0.32862242355231785 1104 68-69 -0.749431103443996 1104 70-71 -0.4240879184578077 1104 72-73 -0.3698144881497285 1104 74-75 -0.569204423545294 1104 76 -0.28609217661883335 1105 1 0.526381637185942 1105 2 0.1038336017913366 1105 3 -0.012980927916991902 1105 4 0.03696195394755364 1105 5 0.17014609570903616 1105 6 0.7829665617652495 1105 7 0.059895449137037815 1105 8 -0.20809641619094776 1105 9 0.28499756657890885 1105 10-11 0.5736155971345767 1105 12-13 -0.46379079721534566 1105 14-15 0.19573088890287238 1105 16-17 0.26240726430434336 1105 18-19 0.12248480031129816 1105 20-21 0.14199752025242418 1105 22-23 0.14408364179003286 1105 24-25 -0.054912568931669625 1105 26-27 0.32649213418066836 1105 28-29 0.1253144741697767 1105 30-31 0.06179974538827082 1105 32-33 -0.10295616546547848 1105 34-35 0.38115561864866976 1105 36-37 8.035902910918935E-4 1105 38-39 0.5184173804834629 1105 40-41 0.3698187399734252 1105 42-43 0.05075879335446132 1105 44-45 0.37120075833164634 1105 46-47 0.22845891792477602 1105 48-49 0.08568337061109332 1105 50-51 -0.059806303044908304 1105 52-53 -0.1080321527579784 1105 54-55 0.04950636263635033 1105 56-57 -0.04587155517170771 1105 58-59 0.16765665946424235 1105 60-61 0.4200558330006814 1105 62-63 0.3692087701607889 1105 64-65 -0.059010223657466554 1105 66-67 0.35080517613923945 1105 68-69 0.03236623627840629 1105 70-71 -0.009555532469711636 1105 72-73 -0.06021986378312505 1105 74-75 -0.06732487959288136 1105 76 0.15914631203505536 1106 1 0.3533378068387414 1106 2 0.28270798178931145 1106 3 0.592748396913457 1106 4 0.2984762772596312 1106 5 0.4636575847261426 1106 6 1.0303296551957573 1106 7 -0.08726519465282223 1106 8 0.24862997311971213 1106 9 0.27062364209262313 1106 10-11 0.5222996744703323 1106 12-13 0.8301863259752551 1106 14-15 0.6331688887004248 1106 16-17 0.27443277295906654 1106 18-19 0.42833559492613915 1106 20-21 0.6496197492229712 1106 22-23 0.5361931670456244 1106 24-25 0.40347896867335464 1106 26-27 0.42893165235052066 1106 28-29 0.213440802745545 1106 30-31 0.18295067159537837 1106 32-33 -0.009814147042568777 1106 34-35 0.1644282170897995 1106 36-37 0.4076767555912184 1106 38-39 0.34736767909667776 1106 40-41 0.3322542091504772 1106 42-43 0.14294929846631277 1106 44-45 0.5552527878892946 1106 46-47 0.37376208626165663 1106 48-49 0.21463366922431248 1106 50-51 0.1751213212085574 1106 52-53 0.20764251066948702 1106 54-55 0.2844390481308352 1106 56-57 0.26342088333422 1106 58-59 0.09799879935694378 1106 60-61 0.5258509539643441 1106 62-63 0.3577602429819251 1106 64-65 0.6115991497608917 1106 66-67 0.11561942859394492 1106 68-69 0.5431314959200719 1106 70-71 0.4160087959036076 1106 72-73 0.335219958061181 1106 74-75 0.2502477492156956 1106 76 0.23366802475901238 1107 1 -0.21850778176662544 1107 2 -0.29460340781258054 1107 3 0.3839809702689365 1107 4 -0.6051927549010223 1107 5 -0.09085446922725282 1107 6 -0.6423363068556824 1107 7 -0.5162349465275966 1107 8 -0.860535057833026 1107 9 -0.014544207321037561 1107 10-11 -0.7606436040036684 1107 12-13 0.038113247310000986 1107 14-15 -0.3933145780065104 1107 16-17 -0.36164855977030896 1107 18-19 -0.23206502916163174 1107 20-21 -0.3258848056111958 1107 22-23 -0.3202364469258896 1107 24-25 -0.3141817087638543 1107 26-27 -0.42294816042314665 1107 28-29 0.0664983295460857 1107 30-31 -0.6482990138442517 1107 32-33 -0.37970694486832457 1107 34-35 -0.06664531454239153 1107 36-37 -0.257027054363995 1107 38-39 -0.2442675315329481 1107 40-41 -0.25357799176552476 1107 42-43 -0.3897398152283884 1107 44-45 -0.2543249462694419 1107 46-47 -0.049369425138017675 1107 48-49 -0.24869614103286608 1107 50-51 -0.41897500975928637 1107 52-53 -0.3078992881125302 1107 54-55 -0.32522424644865566 1107 56-57 -0.3465745869964536 1107 58-59 -0.43134842496234427 1107 60-61 -0.3648028283095499 1107 62-63 -0.09922803020746329 1107 64-65 -0.06441373463187716 1107 66-67 -0.2170394873051933 1107 68-69 -0.11435504089607207 1107 70-71 -0.03730541215920624 1107 72-73 -0.43466277383272 1107 74-75 -0.2807452549616656 1107 76 -0.9668554036973767 1108 1 -0.4191093615192081 1108 2 -0.6606290355253996 1108 3 -0.44936620839817465 1108 4 0.262272787524541 1108 5 0.0916893339474818 1108 6 -0.38961742943534006 1108 7 -0.24402530048355686 1108 8 -0.6786281400120089 1108 9 -0.5783331213622773 1108 10-11 0.04636109648714637 1108 12-13 -0.38969545171321585 1108 14-15 -0.42525403236190584 1108 16-17 -0.5145270046624191 1108 18-19 -0.14181075960683387 1108 20-21 -0.4295707795958137 1108 22-23 -0.2539086448033956 1108 24-25 -0.48484448489173104 1108 26-27 -0.5284011451309709 1108 28-29 -0.7584135795177644 1108 30-31 -0.493213968881534 1108 32-33 -0.8396108582519162 1108 34-35 -0.44435640396248033 1108 36-37 -0.5300639591011631 1108 38-39 -0.7813020687180483 1108 40-41 -0.36224828590964364 1108 42-43 -0.525257942231633 1108 44-45 -0.9172931781510911 1108 46-47 -0.7701626935367258 1108 48-49 -0.5052380612422098 1108 50-51 -0.8395425992174452 1108 52-53 -0.7519693316560989 1108 54-55 -0.9578798506936437 1108 56-57 -0.8565345739818326 1108 58-59 -0.9142286697478497 1108 60-61 -0.7939320260416878 1108 62-63 -0.8846340100071259 1108 64-65 -0.5912944501587276 1108 66-67 -0.7806882173419538 1108 68-69 -0.6232454758472343 1108 70-71 -0.6261024603340388 1108 72-73 -0.9659220761663505 1108 74-75 -0.4749624051766119 1108 76 -0.8945412253833211 1109 1 -0.6073591538582193 1109 2 -0.4764643773830457 1109 3 -0.319438515273454 1109 4 0.030829509612861017 1109 5 -0.18265466158610266 1109 6 -0.4469795221134234 1109 7 -0.1498629572505834 1109 8 -0.45065722616748616 1109 9 -0.30367593220695 1109 10-11 0.07088892305958439 1109 12-13 0.008901304690233758 1109 14-15 -0.0804040248724931 1109 16-17 -0.15512370659741492 1109 18-19 -0.17252075593020777 1109 20-21 -0.028352728749510447 1109 22-23 -0.13679465594219664 1109 24-25 -0.4063962912019008 1109 26-27 -0.16672719330831853 1109 28-29 -0.24526625596151774 1109 30-31 0.03562367426837909 1109 32-33 -0.18347022069863783 1109 34-35 0.19365304631462976 1109 36-37 -0.34502099710653766 1109 38-39 0.10312948485848494 1109 40-41 -0.32900210210583936 1109 42-43 4.265555966753709E-5 1109 44-45 -0.12537289273638663 1109 46-47 -0.13651412401455332 1109 48-49 -0.2307034261127754 1109 50-51 0.23324427933151526 1109 52-53 0.13498138800837012 1109 54-55 0.07620718989898023 1109 56-57 8.061999395181374E-5 1109 58-59 0.1929992943574419 1109 60-61 -0.1905236624102713 1109 62-63 0.0162742514959362 1109 64-65 -0.07111048294873967 1109 66-67 0.17089613387064873 1109 68-69 -0.2142588614702845 1109 70-71 -0.18374370384889005 1109 72-73 -0.1303725575313237 1109 74-75 0.09398104294899667 1109 76 0.3081358992268868 1110 1 -0.1332450326852772 1110 2 -0.3269080513561313 1110 3 0.0913271896098955 1110 4 -0.42923003868197895 1110 5 -0.32613862859948384 1110 6 0.12790637630188684 1110 7 -0.1387466226048346 1110 8 -0.14969348402727434 1110 9 0.39251023456745315 1110 10-11 0.03444497485092768 1110 12-13 0.09271944733190907 1110 14-15 0.03734097522544744 1110 16-17 0.22664648399630494 1110 18-19 -0.19933217980045725 1110 20-21 -0.18469638509316866 1110 22-23 -0.2633298854185995 1110 24-25 -0.046976299428969526 1110 26-27 0.06170613806618519 1110 28-29 -0.005270957142684551 1110 30-31 -0.023596517304753206 1110 32-33 0.06008034638134063 1110 34-35 0.11783670579240635 1110 36-37 -0.04125613451814303 1110 38-39 0.04683773739026975 1110 40-41 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10-11 0.11046352893252731 2116 12-13 0.022521202288032782 2116 14-15 0.019023251350368753 2116 16-17 0.15954551000550765 2116 18-19 -0.027017548272311842 2116 20-21 -0.33085409478679395 2116 22-23 -0.0410354427796662 2116 24-25 -0.31583813848905606 2116 26-27 -0.05260236785753136 2116 28-29 0.04842163993632198 2116 30-31 -0.06797760474726289 2116 32-33 -0.16896998796636353 2116 34-35 -0.04685046490710931 2116 36-37 -0.34793520996549887 2116 38-39 -0.12188721115268919 2116 40-41 -0.6205903481139785 2116 42-43 -0.05447741001011508 2116 44-45 -0.3750642694995818 2116 46-47 -0.334306203022102 2116 48-49 -0.3897961749845251 2116 50-51 -0.19446705169473688 2116 52-53 -0.4238442246846432 2116 54-55 -0.3882303063064825 2116 56-57 -0.24280080797034742 2116 58-59 -0.1104228819929105 2116 60-61 -0.44257271832672984 2116 62-63 -0.44138365229456866 2116 64-65 -0.1754099587951714 2116 66-67 0.004952961021395197 2116 68-69 0.015113975637355281 2116 70-71 -0.2751265383256438 2116 72-73 -0.3322779265637692 2116 74-75 -0.47122952916765115 2116 76 -0.4025272865744185 2117 1 0.5538130073139413 2117 2 0.13525723433856385 2117 3 0.827081631246692 2117 4 -0.027778548995197383 2117 5 0.10122812229667844 2117 6 0.6299435548096568 2117 7 0.5186352112475845 2117 8 0.29110101559075474 2117 9 0.5424779139468967 2117 10-11 0.5012918534625115 2117 12-13 0.49333353912246736 2117 14-15 0.4195959751275069 2117 16-17 0.033520982047271275 2117 18-19 0.7844389510295002 2117 20-21 -0.23531243570921845 2117 22-23 0.45569618654864996 2117 24-25 0.7657648809592672 2117 26-27 0.21056218398105386 2117 28-29 0.20070444000917576 2117 30-31 0.4394698281145324 2117 32-33 0.3586543214259095 2117 34-35 0.45555780821938896 2117 36-37 0.05424640216086374 2117 38-39 0.10038223211123665 2117 40-41 0.22044844734470814 2117 42-43 0.40755181306882804 2117 44-45 0.19056117319768617 2117 46-47 0.2645847770843446 2117 48-49 0.3196628742535239 2117 50-51 0.38251167859891666 2117 52-53 0.12124512427210732 2117 54-55 0.28316689685868823 2117 56-57 0.4936703635836963 2117 58-59 0.2250505764087194 2117 60-61 0.5219305500439404 2117 62-63 0.650889636111323 2117 64-65 0.00581259397434053 2117 66-67 0.30734302031753913 2117 68-69 0.1593675121560878 2117 70-71 0.5039852338800515 2117 72-73 -0.7906289677877396 2117 74-75 -0.10839990943195943 2117 76 0.05905165014263858 2118 1 0.3816518351527698 2118 2 0.11503745411878441 2118 3 0.32891313307819203 2118 4 0.07603097481432641 2118 5 0.19199735306591137 2118 6 0.0670864119525163 2118 7 -0.03506442245204511 2118 8 0.6891962536859921 2118 9 -0.09869424722526787 2118 10-11 -0.7016385494678872 2118 12-13 -0.018204922415996805 2118 14-15 0.055639931171469925 2118 16-17 0.3211766597029495 2118 18-19 0.25997008656062803 2118 20-21 -0.2556054760022519 2118 22-23 -0.07587890502644257 2118 24-25 0.13686378205817107 2118 26-27 0.07096511438398068 2118 28-29 -0.05420398489924594 2118 30-31 0.05126469990941018 2118 32-33 0.5053209880925706 2118 34-35 0.11812191078349343 2118 36-37 0.2205467684612259 2118 38-39 0.07877051049950978 2118 40-41 -0.13533909844283443 2118 42-43 0.017698333215342643 2118 44-45 -0.054090841454332406 2118 46-47 -0.12948115698159057 2118 48-49 -0.3120587374680923 2118 50-51 0.13185233793957707 2118 52-53 -0.04996366693668364 2118 54-55 -0.2577488540570698 2118 56-57 0.18161908153241768 2118 58-59 -0.316158214800069 2118 60-61 -0.2563112081978147 2118 62-63 -0.20954992432823616 2118 64-65 -0.14433392617218033 2118 66-67 0.030419943394456084 2118 68-69 -0.24217094938237693 2118 70-71 -0.2529744730796608 2118 72-73 0.0030706659118990842 2118 74-75 0.10185650082445363 2118 76 0.046743957834948446 2119 1 -0.10838658171491744 2119 2 -0.3965036876662609 2119 3 -0.7166510588274626 2119 4 -0.3458791475835987 2119 5 -0.10180055634175389 2119 6 -0.5688860708492385 2119 7 -0.5402408720187424 2119 8 -0.5574650524875082 2119 9 -0.7025859652633244 2119 10-11 -0.4129402558915416 2119 12-13 -0.4128354950953437 2119 14-15 -0.6780543420350043 2119 16-17 -0.5933663582547055 2119 18-19 -0.47920797115401115 2119 20-21 -0.6764810232192673 2119 22-23 -0.5627081733191304 2119 24-25 -0.5144325638723259 2119 26-27 -0.41275149421067425 2119 28-29 -0.4978348256032561 2119 30-31 -0.5868412591721608 2119 32-33 -0.35491666043775894 2119 34-35 -0.9262433174841718 2119 36-37 -0.2972232663819767 2119 38-39 -0.6810436590707596 2119 40-41 -0.9677271981480118 2119 42-43 -0.8500868896918305 2119 44-45 -0.5006520850887455 2119 46-47 -0.5519076735544459 2119 48-49 -0.7110259491182234 2119 50-51 -0.5980341982884241 2119 52-53 -0.5627984744056391 2119 54-55 -0.5166410188028934 2119 56-57 -0.8851601554907234 2119 58-59 -0.679644322188615 2119 60-61 -0.9886564239576714 2119 62-63 -0.5744744307161653 2119 64-65 -1.1162949732551795 2119 66-67 -0.8390431138734797 2119 68-69 -1.1563208645078973 2119 70-71 -0.7800985684964417 2119 72-73 -0.5542312191948682 2119 74-75 -0.6241686934934236 2119 76 -1.0749606789916406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 39.0 13 676.0 14 1983.0 15 3209.0 16 4124.0 17 4825.0 18 5146.0 19 4610.0 20 3611.0 21 2651.0 22 2050.0 23 1687.0 24 1398.0 25 1477.0 26 1538.0 27 1769.0 28 2136.0 29 2621.0 30 3175.0 31 3935.0 32 5084.0 33 6493.0 34 9229.0 35 13647.0 36 26158.0 37 93205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.10898013347669 17.797053439107312 10.227239705925086 30.866726721490913 2 21.754375768847044 20.85888084929145 28.97984288883077 28.406900493030733 3 19.368649444492874 20.202152287410765 26.50887745909976 33.9203208089966 4 15.601177849456116 17.48515580352386 30.17367467720532 36.73999166981471 5 16.19688296089656 30.084561067038617 32.147734867637226 21.57082110442759 6 30.041457201251465 29.979465124613757 19.12552426892938 20.853553405205396 7 30.482666434196382 24.992977460068385 19.46793362973295 25.05642247600229 8 25.832292060171056 29.06411336801015 20.371177558868254 24.732417012950535 9 24.08295314755083 16.731564621896762 19.54736097792501 39.6381212526274 10-11 26.546653880800857 22.02268522554461 24.47427813132634 26.95638276232819 12-13 25.50005327444086 20.083737734770775 23.85581030424549 30.56039868654288 14-15 24.62707891397631 20.97487383643778 21.81830509787968 32.579742151706235 16-17 25.64922170885034 22.759809761814818 22.849165528530886 28.741803000803962 18-19 24.69706215674309 22.035035209562277 23.50347252491791 29.764430108776725 20-21 24.569687811776557 22.481814043142613 23.950493515047608 28.998004630033225 22-23 25.994294791696937 22.18444580052112 23.07001230155271 28.751247106229233 24-25 25.028574472825195 23.15355631108399 22.403839634246747 29.41402958184407 26-27 24.149061885527757 23.260589505903777 23.773719234010404 28.81662937455807 28-29 25.10025281143754 22.814294985422176 24.65831710884453 27.427135094295764 30-31 24.902653067154855 22.143763500227628 24.428752700045525 28.524830732571992 32-33 24.151725607570782 23.123286742413235 24.419792907718982 28.305194742297 34-35 24.385890990807738 22.671906934394947 24.641123993839535 28.301078080957776 36-37 24.58300642199169 21.912988308681797 24.944788306744545 28.559216962581967 38-39 23.4436598572245 21.762366934976125 26.93071416809539 27.863259039703987 40-41 23.494754889140733 21.278780305892152 26.926113193657436 28.300351611309683 42-43 22.106471391625256 21.032022782088163 27.076734567363108 29.78477125892347 44-45 21.47565358052674 20.946541520161954 28.664555061556197 28.91324983775511 46-47 21.51464078497467 21.42019973072192 28.733085365026785 28.33207411927663 48-49 20.93709741473668 21.855597206482045 28.630653144644953 28.576652234136326 50-51 20.539234204128284 23.044343707319907 27.68285241042629 28.733569678125516 52-53 19.802836137506176 23.379004058543767 27.374102809984596 29.444056993965457 54-55 19.744718565658324 22.947481087573497 27.260773544881296 30.047026801886883 56-57 19.962901616637126 23.364958978680537 26.779608481290985 29.89253092339135 58-59 20.277462974263603 22.8825831323434 26.59750675616773 30.242447137225277 60-61 20.253005162777633 21.92194810100834 27.041379711155667 30.78366702505836 62-63 20.697362430864306 22.468011119828745 26.345906101376414 30.48872034793053 64-65 21.34125669562859 22.26677902730557 25.044556805083353 31.347407471982486 66-67 21.15673340501167 23.0472495859123 23.88559555981751 31.910421449258518 68-69 21.654122957409506 23.1099681321981 23.60348317980608 31.632425730586313 70-71 22.34330049690524 22.595143308245916 23.79527116690398 31.266285027944868 72-73 22.62952953825589 22.231908484196865 24.542324121698194 30.59623785584905 74-75 23.01237904280359 22.06796850027606 24.679142572090004 30.240509884830345 76 22.84214298859927 20.495646025242397 25.32909075058844 31.33312023556989 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 0.5 12 0.0 13 0.5 14 1.0 15 1.0 16 6.5 17 12.0 18 18.0 19 28.0 20 34.5 21 37.0 22 61.5 23 110.5 24 155.0 25 175.0 26 265.5 27 433.5 28 655.0 29 799.0 30 931.5 31 1234.5 32 1766.5 33 2128.0 34 2425.0 35 3139.0 36 3888.5 37 4221.0 38 4781.0 39 5900.5 40 7119.5 41 8145.5 42 8512.0 43 8705.0 44 9521.0 45 10245.5 46 10347.0 47 10465.5 48 10314.0 49 9709.0 50 9374.0 51 8872.0 52 7770.0 53 6500.5 54 5831.0 55 5640.5 56 5071.5 57 4684.5 58 4676.0 59 4457.5 60 4468.0 61 4871.5 62 5046.0 63 5314.5 64 5470.0 65 5471.5 66 5457.5 67 5329.0 68 5195.0 69 4961.0 70 4747.0 71 4633.0 72 4312.0 73 3727.0 74 3089.0 75 2715.0 76 2414.5 77 1758.0 78 1067.0 79 732.0 80 550.5 81 246.0 82 78.0 83 33.0 84 33.0 85 20.5 86 9.0 87 10.0 88 10.0 89 12.5 90 16.5 91 13.0 92 8.0 93 11.0 94 11.5 95 9.0 96 9.0 97 7.5 98 8.5 99 31.0 100 51.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 206478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.132564388030644 #Duplication Level Percentage of deduplicated Percentage of total 1 66.34580469977239 35.91468544786135 2 17.0896525142844 18.502134301971402 3 7.712831986816073 12.5254612242115 4 3.3203869675187723 7.18964245249551 5 1.667936662794884 4.5144844396950505 6 0.9285810731455344 3.015988483893434 7 0.6414178579754496 2.4305115447542107 8 0.4315595732749354 1.8689141110061174 9 0.3573211687613767 1.740844005766343 >10 1.5025278807223634 12.236251857609792 >50 0.0019796149338278174 0.061082130735296565 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0033901916911244783 0.0 2 0.0 0.0 0.0 0.0053274440860527515 0.0 3 0.0 0.0 0.0 0.0053274440860527515 0.0 4 0.0 0.0 0.0 0.018403897751818596 0.0 5 0.0 0.0 0.0 0.021794089442943074 0.0 6 0.0 0.0 0.0 0.02227840254167514 0.0 7 0.0 0.0 0.0 0.02227840254167514 0.0 8 0.0 0.0 0.0 0.02227840254167514 0.0 9 0.0 0.0 0.0 0.024215654936603416 0.0 10 0.0 0.0 0.0 0.02809015972645996 0.0 11 0.0 0.0 0.0 0.02954309902265617 0.0 12 0.0 0.0 0.0 0.02954309902265617 0.0 13 0.0 0.0 0.0 0.03438623000997685 0.0 14 0.0 0.0 0.0 0.035354856207440985 0.0 15 0.0 0.0 0.0 0.03874504789856546 0.0 16 0.0 0.0 0.0 0.04019798719476167 0.0 17 0.0 0.0 0.0 0.04213523958968994 0.0 18 0.0 0.0 0.0 0.045041118182082354 0.0 19 0.0 0.0 0.0 0.0503685622681351 0.0 20 0.0 0.0 0.0 0.052305814663063374 0.0 21 0.0 0.0 0.0 0.054727380156723716 0.0 22 0.0 0.0 0.0 0.06392932903263301 0.0 23 0.0 0.0 0.0 0.06925677311868576 0.0 24 0.0 0.0 0.0 0.0765214695996668 0.0 25 0.0 0.0 0.0 0.08427047917937988 0.0 26 0.0 0.0 0.0 0.09831555904260987 0.0 27 0.0 0.0 0.0 0.1942095525915594 0.0 28 0.0 0.0 0.0 0.3041486260037389 0.0 29 0.0 0.0 0.0 0.4237739613905598 0.0 30 0.0 0.0 0.0 0.5438836098761127 0.0 31 0.0 0.0 0.0 0.6862716609033408 0.0 32 0.0 0.0 0.0 0.8402832263001385 0.0 33 0.0 0.0 0.0 1.0238378907195924 0.0 34 0.0 0.0 0.0 1.3386414048954367 0.0 35 0.0 0.0 0.0 1.7193115004988424 0.0 36 0.0 0.0 0.0 2.2728813723495964 0.0 37 0.0 0.0 0.0 3.1097744069586106 0.0 38 0.0 0.0 0.0 4.002847761020544 0.0 39 0.0 0.0 0.0 4.915777952130493 0.0 40 0.0 0.0 0.0 5.86793750423774 0.0 41 0.0 0.0 0.0 7.06467517120468 0.0 42 0.0 0.0 0.0 8.40380088919885 0.0 43 0.0 0.0 0.0 9.82768139947113 0.0 44 0.0 0.0 0.0 11.063648427435368 0.0 45 0.0 0.0 0.0 12.114607851683957 0.0 46 0.0 0.0 0.0 13.15249082226678 0.0 47 0.0 0.0 0.0 14.141942482976395 0.0 48 0.0 0.0 0.0 15.057778552678736 0.0 49 0.0 0.0 0.0 15.888859830102964 0.0 50 0.0 0.0 0.0 16.699115644281715 0.0 51 0.0 0.0 0.0 17.567973343407047 0.0 52 0.0 0.0 0.0 18.591811234126638 0.0 53 0.0 0.0 0.0 19.745929348405156 0.0 54 0.0 0.0 0.0 20.801247590542335 0.0 55 0.0 0.0 0.0 21.716599347145944 0.0 56 0.0 0.0 0.0 22.492468931314715 0.0 57 0.0 0.0 0.0 23.282867908445454 0.0 58 0.0 0.0 0.0 23.96139055976908 0.0 59 0.0 0.0 0.0 24.68301707687986 0.0 60 0.0 0.0 0.0 25.42014161315007 0.0 61 0.0 0.0 0.0 26.06234078206879 0.0 62 0.0 0.0 0.0 26.800918257635196 0.0 63 0.0 0.0 0.0 27.515764391363728 0.0 64 0.0 0.0 0.0 28.30810062088939 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGGTA 20 0.006944001 52.500004 60 GTAAGCG 20 0.006944001 52.500004 57 TCAACGT 20 0.006944001 52.500004 2 CCATTAG 20 0.006944001 52.500004 32 CACAAGG 30 5.8681413E-4 46.666664 15 GACGAAT 35 0.0012533779 40.0 23 CTTAGGT 40 0.002414854 35.000004 4 CCACAAG 55 2.985016E-4 31.81818 14 CTGACAT 55 2.985016E-4 31.81818 21 CGTATCC 45 0.0043003573 31.111113 56 GTCTACT 45 0.0043003573 31.111113 1 TCTCGTT 50 0.0071968893 27.999998 40 GGAAAGA 50 0.0071968893 27.999998 51 GCCGTAT 50 0.0071968893 27.999998 54 CTGTGTT 50 0.0071968893 27.999998 9 GAAAAGA 65 7.937119E-4 26.923077 34 GAAAAAG 80 8.636905E-5 26.250002 13 CTTGGTG 85 1.3039443E-4 24.705883 16 CGGGACC 85 1.3039443E-4 24.705883 4 GATCTCT 85 1.3039443E-4 24.705883 41 >>END_MODULE