FastQCFastQC Report
Wed 25 May 2016
SRR1780236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206478
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT159557.727215490270149TruSeq Adapter, Index 5 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC40121.9430641521130583RNA PCR Primer, Index 5 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG39771.9261131936574356TruSeq Adapter, Index 5 (95% over 22bp)
CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG35881.737715398250661TruSeq Adapter, Index 5 (96% over 29bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC10220.4949679869041738RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT5630.27266827458615445TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT3430.1661193928650994No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2960.14335667722469222No Hit
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG2390.11575083059696432TruSeq Adapter, Index 5 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG2250.10897044721471538TruSeq Adapter, Index 5 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT207.913243E-570.0000139
GCTTATA308.355866E-658.3333323
ATATCGT252.3864294E-455.99999637
TATCGTA252.3864294E-455.99999638
CTTTATA1450.055.5172422
TCTTTAT1450.053.1034471
GTGCATA200.00694400152.50000464
TTCCACG200.00694400152.5000044
CTTGACA200.00694400152.50000457
TTGCGTG200.00694400152.50000435
GAGCCAT200.00694400152.5000047
TTTATAC1600.048.1250043
TTAATAC950.044.210532
AACTCGT350.001253377940.037
CTTAATA1100.038.1818161
CAGCTTA556.672555E-638.1818161
CCGTCTT33050.035.26474847
TCGTATG33150.035.2639540
TATGCCG33250.035.26315743
CGTATGC33300.035.21021341
Produced by FastQC (version 0.11.5)