##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780236_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 206478 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.72981140847936 34.0 34.0 34.0 34.0 34.0 2 33.74366276310309 34.0 34.0 34.0 34.0 34.0 3 33.75961603657532 34.0 34.0 34.0 34.0 34.0 4 33.77512374199672 34.0 34.0 34.0 34.0 34.0 5 33.771588256375985 34.0 34.0 34.0 34.0 34.0 6 37.54169451466984 38.0 38.0 38.0 37.0 38.0 7 37.532429605091096 38.0 38.0 38.0 37.0 38.0 8 37.46962872557851 38.0 38.0 38.0 37.0 38.0 9 37.48575635176629 38.0 38.0 38.0 37.0 38.0 10-11 37.44118986042096 38.0 38.0 38.0 37.0 38.0 12-13 37.424321235192124 38.0 38.0 38.0 37.0 38.0 14-15 37.35091147725181 38.0 38.0 38.0 37.0 38.0 16-17 37.381277908542316 38.0 38.0 38.0 36.5 38.0 18-19 37.32588459787483 38.0 38.0 38.0 36.5 38.0 20-21 37.356812832359864 38.0 38.0 38.0 36.5 38.0 22-23 37.34443621112176 38.0 38.0 38.0 36.5 38.0 24-25 37.33429953796531 38.0 38.0 38.0 36.5 38.0 26-27 37.23011894729705 38.0 38.0 38.0 36.0 38.0 28-29 37.23274392429218 38.0 38.0 38.0 36.0 38.0 30-31 37.223571034202195 38.0 38.0 38.0 36.0 38.0 32-33 37.1070670967367 38.0 38.0 38.0 36.0 38.0 34-35 37.12621199352958 38.0 38.0 38.0 36.0 38.0 36-37 37.141024709654296 38.0 38.0 38.0 36.0 38.0 38-39 36.52042106180804 38.0 37.0 38.0 34.0 38.0 40-41 36.84108476447854 38.0 37.5 38.0 35.0 38.0 42-43 37.09330049690524 38.0 38.0 38.0 36.0 38.0 44-45 37.165371129127564 38.0 38.0 38.0 36.0 38.0 46-47 37.12206143027345 38.0 38.0 38.0 36.0 38.0 48-49 37.15061895214018 38.0 38.0 38.0 36.0 38.0 50-51 37.0001525586261 38.0 38.0 38.0 36.0 38.0 52-53 37.00058359728398 38.0 38.0 38.0 36.0 38.0 54-55 36.98293522796618 38.0 38.0 38.0 36.0 38.0 56-57 37.02011594455584 38.0 38.0 38.0 36.0 38.0 58-59 36.904529780412446 38.0 38.0 38.0 35.5 38.0 60-61 36.90241091060549 38.0 38.0 38.0 35.0 38.0 62-63 36.88671190151008 38.0 38.0 38.0 35.0 38.0 64-65 36.79569251929988 38.0 38.0 38.0 35.0 38.0 66-67 36.47174517381997 38.0 37.5 38.0 34.0 38.0 68-69 36.37159406813316 38.0 37.0 38.0 34.0 38.0 70-71 35.59445316208022 38.0 37.0 38.0 29.5 38.0 72-73 34.28445645540929 38.0 36.5 38.0 22.0 38.0 74-75 33.89004639719486 38.0 36.0 38.0 20.0 38.0 76 33.21880297174518 38.0 34.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05926838982810523 1101 2 0.010650391737200948 1101 3 -0.07572357027430598 1101 4 0.10078176417728457 1101 5 -0.09227498406707468 1101 6 -0.27798191130508343 1101 7 0.0926326340122543 1101 8 -0.0015487540765661834 1101 9 0.19427229451994776 1101 10-11 0.07631532821633158 1101 12-13 0.018328208197466722 1101 14-15 0.0723988285922843 1101 16-17 0.07092710618375975 1101 18-19 -0.041704221175209 1101 20-21 -0.032932102083513826 1101 22-23 -0.12211073984533982 1101 24-25 -0.15024348544707777 1101 26-27 -0.0885491940259655 1101 28-29 -0.37927408842921295 1101 30-31 -0.17614730086019392 1101 32-33 -0.19019874979873208 1101 34-35 -0.07000743132029896 1101 36-37 -0.04670508388291239 1101 38-39 0.2602268202479934 1101 40-41 -0.018079413327001248 1101 42-43 0.006801747380336565 1101 44-45 -0.11276011969419386 1101 46-47 -0.2754501682240331 1101 48-49 0.0564559925389716 1101 50-51 -0.2724904919044846 1101 52-53 -0.08293145799542145 1101 54-55 -0.118952845059205 1101 56-57 -0.20330726522420406 1101 58-59 -0.23018468974630224 1101 60-61 -0.10398924218055328 1101 62-63 -0.14675106999232668 1101 64-65 -0.15358337763696284 1101 66-67 -0.4711750882761976 1101 68-69 -0.6697095248100382 1101 70-71 -0.39684945159407903 1101 72-73 -0.6134698007412354 1101 74-75 -0.36845526967942277 1101 76 -1.072710469973238 1102 1 0.08093901109524637 1102 2 -0.05435829114692581 1102 3 -0.06142857811007474 1102 4 0.007310163561719207 1102 5 -0.15944026947276768 1102 6 -0.03910376817162131 1102 7 -0.1936589979464074 1102 8 -0.10456093835136926 1102 9 -0.2571635580263205 1102 10-11 -0.10773534311886124 1102 12-13 -0.4199950774623602 1102 14-15 -0.11004939176943651 1102 16-17 -0.1795493935550425 1102 18-19 0.014455685077876979 1102 20-21 -0.049014850675199284 1102 22-23 -0.01757728603404729 1102 24-25 -0.1436307279607334 1102 26-27 -0.11179179239669423 1102 28-29 -0.13812992431564908 1102 30-31 -0.23627274382846508 1102 32-33 -0.08288394936388244 1102 34-35 -0.18418382799397648 1102 36-37 -0.015150212290762965 1102 38-39 -0.1637767658537399 1102 40-41 -0.38657077985765653 1102 42-43 -0.2129758154823307 1102 44-45 -0.04190107890581629 1102 46-47 -0.15653171674229327 1102 48-49 -0.22001445035871114 1102 50-51 -0.21328451695072914 1102 52-53 -0.2726789484301335 1102 54-55 -0.3119140615100875 1102 56-57 -0.21154365145017806 1102 58-59 -0.17981337291279686 1102 60-61 -0.107974191848065 1102 62-63 -0.0023628250644023296 1102 64-65 0.08392174032319843 1102 66-67 -0.14838357632488197 1102 68-69 -0.1488264825533392 1102 70-71 -0.2625718251981155 1102 72-73 -0.05809876627795063 1102 74-75 0.017311859603786672 1102 76 -0.0536187137716837 1103 1 0.028741134764580067 1103 2 0.06187010449779251 1103 3 0.07513253472503578 1103 4 0.06367375086287552 1103 5 0.009188385221833073 1103 6 -0.025240551905447717 1103 7 -0.004636911245647468 1103 8 0.06279945130282982 1103 9 0.07267642470368685 1103 10-11 0.10167848906539234 1103 12-13 0.05324992705752152 1103 14-15 0.1896811019094784 1103 16-17 0.08820294396536354 1103 18-19 0.13250215215020944 1103 20-21 0.03086729512131825 1103 22-23 0.05959124480233413 1103 24-25 -0.025565709139144133 1103 26-27 0.017426200776263556 1103 28-29 -0.09535970965504248 1103 30-31 -0.05786394422783303 1103 32-33 0.0016445663612500994 1103 34-35 -0.11086056882266604 1103 36-37 4.6024145129308636E-4 1103 38-39 0.20525125740677908 1103 40-41 0.15830252856011384 1103 42-43 0.03586891649548818 1103 44-45 0.09813595713180234 1103 46-47 0.011474611090726228 1103 48-49 -0.04712395290199112 1103 50-51 -0.20936503828860964 1103 52-53 -0.23829243791298182 1103 54-55 0.03686219764772858 1103 56-57 -0.3553450060651855 1103 58-59 0.055068637961895206 1103 60-61 0.07808636106152278 1103 62-63 0.0219922486703652 1103 64-65 -0.0803219554550978 1103 66-67 -0.10647686126625189 1103 68-69 -0.12569582426980475 1103 70-71 -0.3607443092436071 1103 72-73 -0.030276201953981285 1103 74-75 -0.11145751854158448 1103 76 -0.06483408946147051 1104 1 0.12743160448296464 1104 2 -0.08599740664714517 1104 3 0.033770541683821875 1104 4 0.055359218950300715 1104 5 -0.07044016213055926 1104 6 0.09692564359308165 1104 7 -0.035034051617508055 1104 8 -0.06696433036510996 1104 9 0.009058532999866031 1104 10-11 0.014158441767364138 1104 12-13 0.013855287501144176 1104 14-15 -0.012723554500226442 1104 16-17 0.004777302451820731 1104 18-19 -0.04464290873920618 1104 20-21 -0.0678265724441971 1104 22-23 -0.07955399277513919 1104 24-25 -0.16792361117241228 1104 26-27 -0.056611189133484174 1104 28-29 0.03165861153665617 1104 30-31 -3.938187055041453E-5 1104 32-33 -0.11756889998646614 1104 34-35 -0.27920180781364223 1104 36-37 -0.020591120405839547 1104 38-39 -0.08930810977817316 1104 40-41 0.06540029141756776 1104 42-43 -0.16885816842350465 1104 44-45 -0.11187478006510787 1104 46-47 -0.2356898854842413 1104 48-49 -0.08355566976618434 1104 50-51 -0.18991746027403877 1104 52-53 -0.17356854697028012 1104 54-55 -0.32445297306011156 1104 56-57 -0.2579423204068192 1104 58-59 -0.13404533942203756 1104 60-61 0.015616941685635766 1104 62-63 -0.06940474219621962 1104 64-65 -0.3599919565424088 1104 66-67 -0.42091873089765386 1104 68-69 -0.28418500552457004 1104 70-71 -0.3418641716987523 1104 72-73 -0.21363762415687404 1104 74-75 -0.5412902765061389 1104 76 -0.5765362749567444 1105 1 -0.03606118027138194 1105 2 -0.004247571881776935 1105 3 0.07669712242848448 1105 4 -0.03766099475316054 1105 5 0.05676985108821242 1105 6 -0.052212382403830304 1105 7 0.05263669083677769 1105 8 0.0866074916221109 1105 9 0.11341108862886529 1105 10-11 0.15366122321702136 1105 12-13 0.07916427626518896 1105 14-15 -0.05610108809793246 1105 16-17 0.04555927535334092 1105 18-19 0.06544122322818424 1105 20-21 0.15872387919713304 1105 22-23 0.12192898805764685 1105 24-25 -0.009274266603128467 1105 26-27 0.19403942223465975 1105 28-29 0.17980522973449098 1105 30-31 0.2525630636020253 1105 32-33 0.1127337547833065 1105 34-35 0.11887940500856331 1105 36-37 -0.06498071613176393 1105 38-39 0.014665783149780509 1105 40-41 0.0873110922547582 1105 42-43 0.01595676878733343 1105 44-45 0.07424137988201807 1105 46-47 0.16515900744371237 1105 48-49 -0.030749434912692664 1105 50-51 0.011885662660525043 1105 52-53 0.17059109722447374 1105 54-55 0.11481985685836804 1105 56-57 0.20401240530369336 1105 58-59 0.19779399743629966 1105 60-61 0.1304481269687372 1105 62-63 0.0713304142259048 1105 64-65 0.12659649906455428 1105 66-67 -0.046617535700470114 1105 68-69 0.2323109186878085 1105 70-71 0.028209770799591638 1105 72-73 0.4392870288504014 1105 74-75 0.1281199735489409 1105 76 0.004180843351257124 1106 1 -0.03214377972476967 1106 2 -0.11360074527989639 1106 3 0.023473111901097354 1106 4 -0.006190198113827705 1106 5 0.0617703572123105 1106 6 0.01945479038693776 1106 7 0.01127622925159244 1106 8 0.015183258400128352 1106 9 0.08077620655577533 1106 10-11 0.12416937181505716 1106 12-13 0.14932826047412107 1106 14-15 0.048231334212019306 1106 16-17 0.08467760716830242 1106 18-19 0.06515273249026166 1106 20-21 0.061406336935768024 1106 22-23 0.0026962721957204394 1106 24-25 0.06650769513389321 1106 26-27 -0.01793041276888374 1106 28-29 0.07423280337555127 1106 30-31 -0.10207303316890659 1106 32-33 -0.01392886289055184 1106 34-35 -0.12052235630331154 1106 36-37 -0.019556078010495526 1106 38-39 -0.09196444258156333 1106 40-41 0.17599415734230917 1106 42-43 -0.14653437404088976 1106 44-45 0.049522278758423965 1106 46-47 -0.02210184891826117 1106 48-49 0.07091571338167313 1106 50-51 -0.002690711466406981 1106 52-53 0.036144290867561324 1106 54-55 0.09675628767120514 1106 56-57 0.08538703836372008 1106 58-59 0.061499838108439064 1106 60-61 0.22056858450492456 1106 62-63 0.08921177873647679 1106 64-65 -0.012734404873093297 1106 66-67 0.12878483296032073 1106 68-69 0.2871848937865096 1106 70-71 0.18898009168227503 1106 72-73 0.2814471665161591 1106 74-75 0.23832849667881106 1106 76 0.3374939317205232 1107 1 -0.03814307510953796 1107 2 -0.18346837827601803 1107 3 -0.04342507119820027 1107 4 -0.08276174172447526 1107 5 -0.055819858701930514 1107 6 -0.032586077296386406 1107 7 -0.032480376516183185 1107 8 0.014609500284471721 1107 9 -0.027613747644231523 1107 10-11 -0.16992695915278233 1107 12-13 0.01862716832050637 1107 14-15 0.0595957712747861 1107 16-17 0.07301622511585748 1107 18-19 -0.23561227833062048 1107 20-21 0.007749887189937965 1107 22-23 -0.08921071859396079 1107 24-25 0.042882953073402064 1107 26-27 0.03890687144269833 1107 28-29 0.08254726308665283 1107 30-31 -0.18686844310397532 1107 32-33 -0.09338429597726616 1107 34-35 -0.33506259486589585 1107 36-37 -0.12318484644373484 1107 38-39 -0.09644881522235949 1107 40-41 0.025251794868104582 1107 42-43 -0.06656054546370882 1107 44-45 -0.07598982552462985 1107 46-47 -0.12984758347963776 1107 48-49 -0.07426522174339567 1107 50-51 0.05176597986179132 1107 52-53 -0.023108816988901992 1107 54-55 0.006273626238829877 1107 56-57 -0.003978304632909158 1107 58-59 0.07711209841342992 1107 60-61 -0.3521225423761436 1107 62-63 -0.02712019569604962 1107 64-65 0.20804671143874742 1107 66-67 0.3346935349584115 1107 68-69 0.19545405680427308 1107 70-71 -0.2928257364922686 1107 72-73 -0.41296120833914784 1107 74-75 -0.3151719311232881 1107 76 -0.5294747129017523 1108 1 -0.06733250004360514 1108 2 0.00134952097148755 1108 3 0.09279224516075146 1108 4 -0.0821940830396457 1108 5 -0.027395388459012793 1108 6 -0.10044205813444762 1108 7 -0.011075490497894691 1108 8 0.049029000052563276 1108 9 0.10726403267185702 1108 10-11 -0.02079194638046289 1108 12-13 -0.1559867199338001 1108 14-15 -0.034095602390010527 1108 16-17 -0.1304149947272677 1108 18-19 0.006790094759587362 1108 20-21 -0.08309280905036331 1108 22-23 -0.23000910976617206 1108 24-25 -0.14834879704601178 1108 26-27 -0.2023203789432344 1108 28-29 -0.07044886714255227 1108 30-31 -0.03826647568205033 1108 32-33 -0.3499314081867766 1108 34-35 -0.04380176954554571 1108 36-37 0.05241552184214271 1108 38-39 -0.03686715198242041 1108 40-41 -0.15389297886452624 1108 42-43 0.027265820205407465 1108 44-45 -0.20066359484752638 1108 46-47 0.02626547751597741 1108 48-49 -0.15712608306161968 1108 50-51 -0.13330459672713602 1108 52-53 -0.0927716138847785 1108 54-55 -0.09035815018672366 1108 56-57 -0.09602727557847857 1108 58-59 -0.15519529568707213 1108 60-61 -0.08086649369594312 1108 62-63 -0.2433981546169477 1108 64-65 -0.022440021270163868 1108 66-67 -0.22254307487316538 1108 68-69 -0.26562497928359363 1108 70-71 0.14711666691922431 1108 72-73 -0.5316728308045882 1108 74-75 -1.0021098971053064 1108 76 -0.11248095022462223 1109 1 0.023514075933086076 1109 2 -0.12323345491260085 1109 3 0.06749845592644732 1109 4 0.03399394207031747 1109 5 0.04790044334239951 1109 6 -0.1251843542522053 1109 7 0.05111386908923521 1109 8 0.08724543046230337 1109 9 -0.020144494364920718 1109 10-11 0.06928866693435509 1109 12-13 0.14778385892971357 1109 14-15 0.12341695939764463 1109 16-17 -0.011258438767740131 1109 18-19 -0.002622735285214617 1109 20-21 0.03547821100764992 1109 22-23 -0.08050341100397418 1109 24-25 0.13456036318656572 1109 26-27 -0.038972883811361214 1109 28-29 -0.052630873488126895 1109 30-31 -0.08333231442819056 1109 32-33 -0.18166473062641586 1109 34-35 -0.11567630145724905 1109 36-37 0.10347629502187772 1109 38-39 0.013431062813936023 1109 40-41 -0.009398378050221368 1109 42-43 -0.05684028434679789 1109 44-45 0.16728784652399042 1109 46-47 0.19505981824340068 1109 48-49 -0.014214471748516644 1109 50-51 0.1687774600017562 1109 52-53 0.11094381566707767 1109 54-55 0.008304649219574856 1109 56-57 0.004810857787539646 1109 58-59 0.0178952445038405 1109 60-61 0.036928350864691595 1109 62-63 -0.05957052004581698 1109 64-65 0.033107590968903367 1109 66-67 0.03681869099417412 1109 68-69 -0.03631277971116731 1109 70-71 0.04453894724112928 1109 72-73 -0.4886053035363176 1109 74-75 -0.10574634739602828 1109 76 -0.0952553010287076 1110 1 0.026217255106850246 1110 2 -0.0348610430107783 1110 3 -0.041931329973927234 1110 4 0.05151563606259657 1110 5 0.010163670311506223 1110 6 -0.10060109437483078 1110 7 -0.056758650547990896 1110 8 0.12231820082919143 1110 9 -0.035560450957348166 1110 10-11 -0.2106911571631187 1110 12-13 0.14052641049579506 1110 14-15 -0.1716752033415787 1110 16-17 -0.03875545155887039 1110 18-19 0.020892964701076266 1110 20-21 -0.06080785586665627 1110 22-23 -0.22555443840793998 1110 24-25 0.11563810897018811 1110 26-27 0.11069607468112963 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0.21005654913268046 2115 50-51 0.1429294850012397 2115 52-53 -7.519273845915109E-4 2115 54-55 0.0824080411195709 2115 56-57 0.2043721251454187 2115 58-59 0.019462885901994298 2115 60-61 0.25406479257740955 2115 62-63 0.07296502350667566 2115 64-65 0.08565720707783697 2115 66-67 0.16192475254091931 2115 68-69 0.07184412929320416 2115 70-71 -0.1942081813365064 2115 72-73 -0.30900430190141037 2115 74-75 -0.4857260663588008 2115 76 -0.03805449806519334 2116 1 0.07746748900252243 2116 2 0.1509555236962612 2116 3 0.0702204116870746 2116 4 0.14344894379051 2116 5 0.0652744137550414 2116 6 0.24726823919485952 2116 7 0.054250695430482665 2116 8 0.04979577146568204 2116 9 0.06886042336928 2116 10-11 0.18625687368155752 2116 12-13 0.15186173317333385 2116 14-15 0.1462702571128176 2116 16-17 -0.20447176319653693 2116 18-19 -8.519462216014517E-4 2116 20-21 -0.0019006164264325776 2116 22-23 0.17414617513733077 2116 24-25 0.006426066543980369 2116 26-27 0.10763327246331045 2116 28-29 0.12145792905371877 2116 30-31 0.17811710171736195 2116 32-33 0.04817357103509323 2116 34-35 0.2435560867806572 2116 36-37 0.1214523337161495 2116 38-39 -0.14832798844787476 2116 40-41 -0.030511243085733497 2116 42-43 0.1827424165618723 2116 44-45 -0.0455053455509713 2116 46-47 0.14627204984237352 2116 48-49 0.22320280710523122 2116 50-51 0.24898914518582416 2116 52-53 0.05723831306655569 2116 54-55 0.18320295533334985 2116 56-57 -0.04197844867027811 2116 58-59 0.03403978136660868 2116 60-61 0.060611389741104915 2116 62-63 0.23043919399704293 2116 64-65 0.01281434829996897 2116 66-67 0.21635140505727435 2116 68-69 0.14321993435194003 2116 70-71 0.22399577034435936 2116 72-73 -0.2531105956659161 2116 74-75 0.062478613646611336 2116 76 -0.22734604637912526 2117 1 -0.04058848816947602 2117 2 -0.13788546956461545 2117 3 -0.11198872356073508 2117 4 0.10542251349888687 2117 5 0.1321495275914799 2117 6 0.1550354259675757 2117 7 0.1756559936313593 2117 8 -0.5079926647757986 2117 9 -0.020144494364920718 2117 10-11 0.1681897658354501 2117 12-13 0.08093404207990318 2117 14-15 0.25711659309727963 2117 16-17 0.13159870408940577 2117 18-19 0.150307667645194 2117 20-21 0.13804231357175212 2117 22-23 0.12187754137698903 2117 24-25 0.05489003351623012 2117 26-27 0.06725422241574819 2117 28-29 -0.5498836207408715 2117 30-31 -0.09432132541719795 2117 32-33 -0.14045593941762036 2117 34-35 0.12791344213248834 2117 36-37 -0.07326363171804928 2117 38-39 -0.030524981142107777 2117 40-41 -0.00573537438722127 2117 42-43 0.1693501918436766 2117 44-45 -0.018609589373447477 2117 46-47 0.11722099040457579 2117 48-49 0.10300164546760016 2117 50-51 0.2008287420530408 2117 52-53 -0.052975598252331224 2117 54-55 0.28852442943934875 2117 56-57 -0.14354079056410995 2117 58-59 0.2862102628188623 2117 60-61 0.014950328886669695 2117 62-63 0.17303021255491302 2117 64-65 -0.009932702071388633 2117 66-67 0.18333883751432012 2117 68-69 -0.07843732183570751 2117 70-71 -0.02872112601894372 2117 72-73 -0.1735869885179966 2117 74-75 0.1479166562669718 2117 76 -0.6593578651312768 2118 1 -0.03538702296801688 2118 2 -0.05429572597487464 2118 3 -0.19279458436659525 2118 4 0.05121005928643285 2118 5 0.04035465579661235 2118 6 -0.061008530076385625 2118 7 0.05287211084747412 2118 8 -0.03531867210180195 2118 9 0.08329872907830094 2118 10-11 0.06661467426035728 2118 12-13 0.016355287501141902 2118 14-15 0.06697007295075963 2118 16-17 0.08321042570111814 2118 18-19 -0.12980222246469708 2118 20-21 -0.0018111662817332785 2118 22-23 0.0869691164685591 2118 24-25 0.004010912637113506 2118 26-27 -0.13468716952564108 2118 28-29 0.04301015215289539 2118 30-31 0.10670431560843952 2118 32-33 0.0956979067362198 2118 34-35 -0.10150047728142653 2118 36-37 0.05171805326362744 2118 38-39 0.004200293583167536 2118 40-41 -0.09273171138355707 2118 42-43 0.015833708327193108 2118 44-45 0.0028556120917571093 2118 46-47 0.07231256549614784 2118 48-49 0.09325805572401435 2118 50-51 -0.06668041545611203 2118 52-53 0.10878264350591138 2118 54-55 0.10694933786425764 2118 56-57 0.0441515171281921 2118 58-59 -0.061408784800185856 2118 60-61 -0.17255882862248484 2118 62-63 -0.1360906665659698 2118 64-65 -0.02143453357322045 2118 66-67 -0.08622160204611617 2118 68-69 -0.021257834656218222 2118 70-71 0.06856825127042754 2118 72-73 -0.12267123760224763 2118 74-75 -0.06303572468540608 2118 76 -0.5125446783180863 2119 1 0.04781497828276571 2119 2 0.07970588217307295 2119 3 0.08014028564144837 2119 4 0.06316395904521244 2119 5 0.017032936865135184 2119 6 0.05564294852631235 2119 7 0.06496180976644439 2119 8 0.12329474699942011 2119 9 0.045074839147090984 2119 10-11 -0.02440828651869964 2119 12-13 0.09699318618782371 2119 14-15 -0.502085586836607 2119 16-17 0.009759161518154258 2119 18-19 0.044594275346433676 2119 20-21 0.17471702097815722 2119 22-23 0.2109734048630969 2119 24-25 0.06826893104147302 2119 26-27 0.19298012253188546 2119 28-29 0.19276803654493335 2119 30-31 0.06868233758646625 2119 32-33 -0.13449239077115038 2119 34-35 0.09403065824970014 2119 36-37 0.042120983666563916 2119 38-39 -0.1071800334069124 2119 40-41 0.10784007455408329 2119 42-43 0.2262830963375535 2119 44-45 0.23507574967774758 2119 46-47 0.15260024529602134 2119 48-49 0.16272647348511526 2119 50-51 0.07385206019831259 2119 52-53 0.12307193145373674 2119 54-55 0.18723344400202535 2119 56-57 0.21476350676457656 2119 58-59 0.3368222524552422 2119 60-61 0.24123014662990272 2119 62-63 0.09188574726410792 2119 64-65 0.011062562826310796 2119 66-67 -0.031773286134388457 2119 68-69 -0.032984806870992145 2119 70-71 -0.2520303371330357 2119 72-73 -0.38957019230608125 2119 74-75 -0.5791672354510666 2119 76 -1.5920836759058652 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 2.0 21 4.0 22 8.0 23 26.0 24 40.0 25 100.0 26 167.0 27 370.0 28 583.0 29 986.0 30 1563.0 31 2351.0 32 3889.0 33 6727.0 34 11133.0 35 19372.0 36 29550.0 37 129605.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.60895591782175 14.167611077209195 11.17165024845262 36.05178275651643 2 21.287013628570598 17.766057400788462 34.739778572051264 26.20715039858968 3 19.66989219190422 16.584817752980946 32.43057371729676 31.314716337818073 4 15.028719766754811 22.72493921870611 30.968916785323376 31.277424229215704 5 15.631689574676235 28.636949214928464 38.61476767500654 17.11659353538876 6 29.585036517561363 36.86142699393634 15.978128208605359 17.575408279896937 7 28.332800588924727 24.43650170962524 19.98566433227753 27.2450333691725 8 24.44667228469861 36.37772547196311 14.887784655023781 24.287817588314496 9 22.37429653522409 15.76342273753136 15.674309127364658 46.18797159987989 10-11 26.226280765989596 25.77974408895863 23.193269985180017 24.800705159871754 12-13 25.238039888026815 18.034609014035393 25.61265606989607 31.114695028041726 14-15 22.484235608636272 17.526322416916088 19.73842249537481 40.25101947907283 16-17 28.64929919894613 24.534332955569116 18.396633055337613 28.41973479014713 18-19 30.601565299935103 22.93876345179632 18.748244365017097 27.711426883251484 20-21 24.90555894574725 20.634886041127867 18.847286393707805 35.61226861941708 22-23 23.574182237332792 25.510708162612968 18.921144141264445 31.9939654587898 24-25 28.55122579645289 25.8049283700927 18.83348347039394 26.81036236306047 26-27 29.069925125194935 28.441044566491346 19.090895882370035 23.398134425943685 28-29 25.11526651749823 26.395548193996454 20.561996919768692 27.927188368736623 30-31 23.785584904929337 27.07043849707959 19.28704268735652 29.85693391063455 32-33 22.237962397931014 31.923982216023017 20.558848884626933 25.27920650141904 34-35 28.04608723447534 23.316769825356698 25.0527901277618 23.584352812406166 36-37 30.424064549249795 22.735594106878214 23.5327734673912 23.307567876480785 38-39 23.194965081025583 22.87265471381939 26.823680973275604 27.108699231879424 40-41 22.611609953602805 18.52400740030415 27.99668729840467 30.86769534768837 42-43 27.393959647032613 18.04017861467081 27.168269743023473 27.397591995273103 44-45 21.897732446071736 23.351398211916038 30.258913782582162 24.49195555943006 46-47 25.335871133970688 19.63526380534488 27.532957506368717 27.495907554315714 48-49 24.862939393058824 18.439252608026035 24.19531378645667 32.50249421245847 50-51 22.321022094363563 19.38124158505991 28.334011371671558 29.963724948904968 52-53 18.448696713451312 19.675219635990274 35.6563411114017 26.219742539156716 54-55 19.076850802506804 20.36536580168347 30.71150437334728 29.84627902246244 56-57 23.027877061963018 21.38460271796511 25.002179408944293 30.585340811127576 58-59 19.62678832611707 21.395984075785314 31.42029659334166 27.556931004755953 60-61 23.42356086362712 25.449926868722088 25.463487635486587 25.6630246321642 62-63 19.27614564263505 33.86849930743227 20.756932942008348 26.09842210792433 64-65 18.565658326795106 36.256647197280095 19.320460291169034 25.85723418475576 66-67 18.549191681438217 35.70259301233062 18.731293406561473 27.0169218996697 68-69 18.180871569852478 36.32420887455322 18.85140305504703 26.643516500547275 70-71 18.35352918955046 35.22966127141874 19.135452687453387 27.28135685157741 72-73 18.81507957264212 33.256569707184305 19.175166361549415 28.75318435862416 74-75 19.025997927139937 32.92747895659586 19.629452048160097 28.41707106810411 76 19.143443853582465 33.10134735904067 19.35605730392584 28.399151483451025 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.0 15 3.5 16 6.5 17 12.0 18 15.0 19 16.0 20 23.5 21 33.0 22 43.5 23 74.5 24 97.5 25 100.0 26 173.0 27 301.0 28 441.5 29 527.0 30 625.5 31 917.0 32 1340.5 33 1571.0 34 1792.0 35 2332.0 36 2905.0 37 3159.0 38 3778.0 39 8588.0 40 14913.5 41 15691.5 42 14335.0 43 12803.0 44 10689.5 45 9560.0 46 9012.0 47 9036.0 48 8664.5 49 8030.5 50 7792.0 51 7222.5 52 6030.0 53 5022.5 54 4638.0 55 4562.5 56 4235.0 57 3984.0 58 3985.0 59 3870.5 60 3922.5 61 4222.0 62 4355.0 63 4570.0 64 4751.0 65 4763.5 66 4970.5 67 5131.0 68 5007.5 69 4809.5 70 4606.5 71 4478.0 72 4303.5 73 3792.0 74 3122.5 75 2790.0 76 2405.5 77 1648.5 78 972.0 79 668.0 80 504.0 81 221.0 82 66.0 83 30.0 84 19.5 85 5.0 86 1.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 9.686261974641367E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 206478.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.505206365811375 #Duplication Level Percentage of deduplicated Percentage of total 1 51.67355371900827 19.380272958862445 2 24.149018595041323 18.114278518776818 3 11.151859504132231 12.547583761950426 4 4.894111570247934 7.342186576778155 5 2.3889462809917354 4.479896163271632 6 1.4010847107438016 3.152878272745765 7 0.9077995867768595 2.383304758860508 8 0.640495867768595 1.9217543757688471 9 0.49457644628099173 1.6694272513294397 >10 2.2611053719008267 13.521053090401884 >50 0.015495867768595042 0.38212303489960187 >100 0.014204545454545454 1.0034967405728454 >500 0.0012913223140495868 0.27266827458615445 >1k 0.005165289256198347 6.1018607309253285 >5k 0.0 0.0 >10k+ 0.0012913223140495868 7.727215490270149 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 15955 7.727215490270149 TruSeq Adapter, Index 5 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 4012 1.9430641521130583 RNA PCR Primer, Index 5 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 3977 1.9261131936574356 TruSeq Adapter, Index 5 (95% over 22bp) CACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTTCTG 3588 1.737715398250661 TruSeq Adapter, Index 5 (96% over 29bp) TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 1022 0.4949679869041738 RNA PCR Primer, Index 5 (95% over 21bp) ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT 563 0.27266827458615445 TruSeq Adapter, Index 5 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT 343 0.1661193928650994 No Hit TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 296 0.14335667722469222 No Hit CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 239 0.11575083059696432 TruSeq Adapter, Index 5 (95% over 22bp) CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 225 0.10897044721471538 TruSeq Adapter, Index 5 (95% over 22bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0038745047898565465 0.0 2 0.0 0.0 0.0 0.00581175718478482 0.0 3 0.0 0.0 0.0 0.00581175718478482 0.0 4 0.0 0.0 0.0 0.0198568370480148 0.0 5 0.0 0.0 0.0 0.02324702873913928 0.0 6 0.0 0.0 0.0 0.023731341837871348 0.0 7 0.0 0.0 0.0 0.023731341837871348 0.0 8 0.0 0.0 0.0 0.023731341837871348 0.0 9 0.0 0.0 0.0 0.02566859423279962 0.0 10 0.0 0.0 0.0 0.02954309902265617 0.0 11 0.0 0.0 0.0 0.03099603831885237 0.0 12 0.0 0.0 0.0 0.03099603831885237 0.0 13 0.0 0.0 0.0 0.03632348240490512 0.0 14 0.0 0.0 0.0 0.03729210860236926 0.0 15 0.0 0.0 0.0 0.04068230029349374 0.0 16 0.0 0.0 0.0 0.04213523958968994 0.0 17 0.0 0.0 0.0 0.04407249198461822 0.0 18 0.0 0.0 0.0 0.046978370577010625 0.0 19 0.0 0.0 0.0 0.05327444086052752 0.0 20 0.0 0.0 0.0 0.05521169325545579 0.0 21 0.0 0.0 0.0 0.05763325874911613 0.0 22 0.0 0.0 0.0 0.06150776353897267 0.0 23 0.0 0.0 0.0 0.06828814692122163 0.0 24 0.0 0.0 0.0 0.07409990410600645 0.0 25 0.0 0.0 0.0 0.08184891368571955 0.0 26 0.0 0.0 0.0 0.09589399354894952 0.0 27 0.0 0.0 0.0 0.19178798709789904 0.0 28 0.0 0.0 0.0 0.3022113736088106 0.0 29 0.0 0.0 0.0 0.42522690068675595 0.0 30 0.0 0.0 0.0 0.5506639932583617 0.0 31 0.0 0.0 0.0 0.6954736097792501 0.0 32 0.0 0.0 0.0 0.8519067406697082 0.0 33 0.0 0.0 0.0 1.0417574753726788 0.0 34 0.0 0.0 0.0 1.3628570598320402 0.0 35 0.0 0.0 0.0 1.751276165015159 0.0 36 0.0 0.0 0.0 2.3072676023595733 0.0 37 0.0 0.0 0.0 3.1591743430292816 0.0 38 0.0 0.0 0.0 4.057575141177268 0.0 39 0.0 0.0 0.0 4.97825434186693 0.0 40 0.0 0.0 0.0 5.940100155948818 0.0 41 0.0 0.0 0.0 7.148461337285328 0.0 42 0.0 0.0 0.0 8.486134115983301 0.0 43 0.0 0.0 0.0 9.923091079921347 0.0 44 0.0 0.0 0.0 11.155667916194462 0.0 45 0.0 0.0 0.0 12.221641046503743 0.0 46 0.0 0.0 0.0 13.282286732726973 0.0 47 0.0 0.0 0.0 14.278034463720106 0.0 48 0.0 0.0 0.0 15.215180309766659 0.0 49 0.0 0.0 0.0 16.050620405079474 0.0 50 0.0 0.0 0.0 16.87346835982526 0.0 51 0.0 0.0 0.0 17.746200563740448 0.0 52 0.0 0.0 0.0 18.765679636571452 0.0 53 0.0 0.0 0.0 19.92657813423222 0.0 54 0.0 4.843130987320683E-4 0.0 20.988192446652914 0.0 55 0.0 4.843130987320683E-4 0.0 21.909355960441307 0.0 56 0.0 4.843130987320683E-4 0.0 22.695880432782186 0.0 57 0.0 4.843130987320683E-4 0.0 23.498387237381223 0.0 58 0.0 4.843130987320683E-4 0.0 24.178362828001045 0.0 59 0.0 4.843130987320683E-4 0.0 24.9101599201852 0.0 60 0.0 4.843130987320683E-4 0.0 25.650190335047803 0.0 61 0.0 4.843130987320683E-4 0.0 26.2889993122754 0.0 62 0.0 4.843130987320683E-4 0.0 27.037263049816445 0.0 63 0.0 4.843130987320683E-4 0.0 27.745328800162728 0.0 64 0.0 4.843130987320683E-4 0.0 28.55074148335416 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGTAT 20 7.913243E-5 70.00001 39 GCTTATA 30 8.355866E-6 58.333332 3 ATATCGT 25 2.3864294E-4 55.999996 37 TATCGTA 25 2.3864294E-4 55.999996 38 CTTTATA 145 0.0 55.517242 2 TCTTTAT 145 0.0 53.103447 1 GTGCATA 20 0.006944001 52.500004 64 TTCCACG 20 0.006944001 52.500004 4 CTTGACA 20 0.006944001 52.500004 57 TTGCGTG 20 0.006944001 52.500004 35 GAGCCAT 20 0.006944001 52.500004 7 TTTATAC 160 0.0 48.125004 3 TTAATAC 95 0.0 44.21053 2 AACTCGT 35 0.0012533779 40.0 37 CTTAATA 110 0.0 38.181816 1 CAGCTTA 55 6.672555E-6 38.181816 1 CCGTCTT 3305 0.0 35.264748 47 TCGTATG 3315 0.0 35.26395 40 TATGCCG 3325 0.0 35.263157 43 CGTATGC 3330 0.0 35.210213 41 >>END_MODULE