FastQCFastQC Report
Wed 25 May 2016
SRR1780235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484258
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG353.2196415E-431.42089330
CCTAAGT602.9561852E-729.31405431
CACAATG554.978383E-627.98159832
AAGTGTA655.888778E-727.09270734
GTTAAGT508.251714E-526.4372616
TTGTATA450.001404899224.420812
AGTTAAG551.5765351E-424.03387315
CTTTATA551.6021715E-423.9767972
CTGACTT752.0312327E-623.50465421
GACGAAA952.6868292E-823.14984723
CTATAAC856.152255E-620.6858634
GGTCCTA856.152255E-620.6858631
CGACAAC654.9240986E-420.30902924
GAAGATA550.004438047820.03237321
AAGTTGG550.00446470820.01165840
CTAAGTG550.00449687319.98685532
ATAACAT550.00449687319.98685531
GTATAAG1705.456968E-1219.3929981
CTAATTC951.5972435E-518.5217930
CTATACA600.00744727918.3156071
Produced by FastQC (version 0.11.5)