FastQCFastQC Report
Wed 25 May 2016
SRR1780235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences484258
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGT200544.141180940738201No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC52701.0882628681405366No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCG48501.001532241078103No Hit
CACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTTCTG42770.8832068855857828TruSeq Adapter, Index 9 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCC10500.21682656765608416No Hit
CTTATACACATCTCCGAGCCCACGAGACTGGATCTGATATCGTATGCCGT7310.15095259138723574No Hit
ATACACATCTCCGAGCCCACGAGACTGGATCTGATCTCGTATGCCGTCTT5960.12307488983145348TruSeq Adapter, Index 9 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAAC207.854868E-443.99669623
AATTCAG302.525976E-643.99669618
GTATCCA301.3005576E-436.66391418
GTCTTCT6100.036.42725443
TCTTCTG6250.036.25702344
CCGTCTT6200.035.8397241
AAAAAGC250.002352271235.19735715
CGTCTTC6550.034.26050642
CTTTATA1700.033.651482
TCTTTAT1800.033.0658041
GGATTAC401.7932165E-533.0658041
GCTTATA401.8161969E-533.0043373
AATAACA401.8187657E-532.9975228
GCCGTCT7000.031.74047340
ATGCCGT29700.031.4082944
TTACACC454.060924E-529.3371894
TACGGTC300.005743764329.33113110
TATGACG300.005743764329.33113129
AATGCTC300.005743764329.33113117
AAATTCA454.066651E-529.33113117
Produced by FastQC (version 0.11.5)