FastQCFastQC Report
Wed 25 May 2016
SRR1780234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156339
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG150.002221299870.015
AACGGGG200.006940860852.511
GGGACGT200.006940860852.554
CTCGGTT200.006940860852.544
TAGATCT503.461555E-642.00000439
GTACATG400.002413065235.01
TTGTGAT400.002413065235.010
AAGAGAC450.004297191331.1111095
GCGAACT450.004297191331.11110928
AATAGAG450.004297191331.1111095
TTCAACA500.007191620828.00000262
GTATAGG500.007191620828.0000021
CGGTCGT500.007191620828.00000210
TCAAGCC500.007191620828.00000221
GTAGATC755.557688E-527.99999838
TAATACA657.9284416E-426.9230774
TCTACAC657.9284416E-426.9230773
AGATCTC808.623729E-526.2540
ACATGCT750.001825987723.3333323
GGCATCA800.002656950121.87519
Produced by FastQC (version 0.11.5)