##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780234_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156339 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.846071677572453 34.0 32.0 34.0 21.0 34.0 2 30.991799870793596 34.0 33.0 34.0 21.0 34.0 3 30.83356680035052 34.0 33.0 34.0 21.0 34.0 4 30.72871772238533 34.0 32.0 34.0 21.0 34.0 5 30.68361701175011 34.0 32.0 34.0 21.0 34.0 6 33.91455107170955 38.0 34.0 38.0 21.0 38.0 7 34.17762682376119 38.0 35.0 38.0 21.0 38.0 8 34.19650886854848 38.0 35.0 38.0 22.0 38.0 9 34.3403117584224 38.0 36.0 38.0 21.0 38.0 10-11 34.12808704162109 38.0 35.5 38.0 21.0 38.0 12-13 34.176088499990406 38.0 35.0 38.0 21.5 38.0 14-15 34.103972777106165 38.0 35.0 38.0 21.0 38.0 16-17 34.13988192325651 38.0 36.0 38.0 21.0 38.0 18-19 34.146159947294024 38.0 35.5 38.0 21.0 38.0 20-21 34.17472287784878 38.0 36.0 38.0 21.5 38.0 22-23 34.161162601782024 38.0 36.0 38.0 21.0 38.0 24-25 34.1828174671707 38.0 36.0 38.0 21.5 38.0 26-27 34.167597336557094 38.0 36.0 38.0 21.0 38.0 28-29 34.224966259218746 38.0 36.0 38.0 21.0 38.0 30-31 34.24536743870691 38.0 36.0 38.0 21.5 38.0 32-33 34.303750183895254 38.0 36.0 38.0 22.0 38.0 34-35 34.264127952718134 38.0 36.0 38.0 22.0 38.0 36-37 34.206340068696875 38.0 36.0 38.0 21.5 38.0 38-39 34.18505299381472 38.0 36.0 38.0 21.0 38.0 40-41 34.24045823498935 38.0 36.0 38.0 21.0 38.0 42-43 34.29152994454358 38.0 36.0 38.0 21.5 38.0 44-45 34.35436775212839 38.0 36.0 38.0 22.0 38.0 46-47 34.299873991774284 38.0 36.0 38.0 21.5 38.0 48-49 34.26606604877861 38.0 36.0 38.0 21.5 38.0 50-51 34.20370157158483 38.0 36.0 38.0 21.0 38.0 52-53 34.25763245255503 38.0 36.0 38.0 22.0 38.0 54-55 34.226830797177925 38.0 36.0 38.0 21.5 38.0 56-57 34.18347629190413 38.0 36.0 38.0 21.0 38.0 58-59 34.23509169177237 38.0 36.0 38.0 21.5 38.0 60-61 34.24456469594919 38.0 36.0 38.0 22.0 38.0 62-63 34.13844274301358 38.0 36.0 38.0 21.5 38.0 64-65 34.13251971676932 38.0 36.0 38.0 21.5 38.0 66-67 34.06003300520024 38.0 35.5 38.0 22.0 38.0 68-69 34.00659464369095 38.0 35.0 38.0 21.0 38.0 70-71 34.026922265077815 38.0 35.5 38.0 21.0 38.0 72-73 34.07257945874031 38.0 35.5 38.0 21.5 38.0 74-75 34.10397597528448 38.0 36.0 38.0 22.0 38.0 76 32.59816168710302 37.0 31.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2958875435252466 1101 2 -0.14324435885262332 1101 3 0.09516065805233609 1101 4 0.2664828377394528 1101 5 -0.9576492575886846 1101 6 -0.24550727958331464 1101 7 -0.5980855446324895 1101 8 -0.31473605824456286 1101 9 -0.08806619712618158 1101 10-11 -0.25084015205142407 1101 12-13 0.0694527250751662 1101 14-15 -0.39089242017374204 1101 16-17 -0.4449266167943051 1101 18-19 -0.2546426914721991 1101 20-21 -0.3746669580863795 1101 22-23 -0.18796451221293609 1101 24-25 -0.4755103103240259 1101 26-27 -0.5604202450999196 1101 28-29 -0.6969874046395006 1101 30-31 -0.5415266980661642 1101 32-33 -0.707927168378987 1101 34-35 -0.6071366706566366 1101 36-37 -0.07843103923526229 1101 38-39 -0.2594110444163178 1101 40-41 -0.23739792367564405 1101 42-43 -0.3941368609277731 1101 44-45 0.0016444942805122764 1101 46-47 -0.20826151972965334 1101 48-49 -0.413325940253074 1101 50-51 -0.4915342494217825 1101 52-53 -0.16157343091293086 1101 54-55 0.03358434668233912 1101 56-57 -0.27895523232686514 1101 58-59 -0.16162109283850157 1101 60-61 -0.12235708479491336 1101 62-63 -0.4181244473826524 1101 64-65 -0.39558609913140685 1101 66-67 -0.27267868429682096 1101 68-69 -0.49211800082338897 1101 70-71 -0.09361662265496307 1101 72-73 0.1287010294686297 1101 74-75 -0.1768970397329852 1101 76 -1.8575532320475503 1102 1 0.12582516499471552 1102 2 -0.0655018219908925 1102 3 0.8707338609673805 1102 4 0.79956760155806 1102 5 0.10919213407778017 1102 6 0.7828018020814582 1102 7 0.029405194539002366 1102 8 -0.04112397195542883 1102 9 0.2853812522116428 1102 10-11 0.1551684913885225 1102 12-13 0.4863383023940031 1102 14-15 0.19032746700151648 1102 16-17 0.49643357495931895 1102 18-19 0.05580691267277871 1102 20-21 0.12533909474271354 1102 22-23 0.2618635874408426 1102 24-25 0.634620915010693 1102 26-27 0.44099006407859065 1102 28-29 0.32686982122786645 1102 30-31 0.3512383553197864 1102 32-33 0.4659312680542058 1102 34-35 0.22441914853325784 1102 36-37 0.3118432749792035 1102 38-39 0.4450414166639902 1102 40-41 -0.03298177956989434 1102 42-43 0.013717895385326528 1102 44-45 0.17042158069070013 1102 46-47 -0.026325582872765096 1102 48-49 0.17010322954088508 1102 50-51 0.38851381004121777 1102 52-53 0.4784194267487436 1102 54-55 0.18318001769897307 1102 56-57 0.24039868869581937 1102 58-59 0.2978310050720552 1102 60-61 -0.06811465688411289 1102 62-63 0.23072369924107505 1102 64-65 0.3685714681155261 1102 66-67 0.11616110942276237 1102 68-69 0.35932438834930025 1102 70-71 0.41398270427043826 1102 72-73 0.3070597443319656 1102 74-75 0.2213488503960619 1102 76 0.6135626675239081 1103 1 0.31778809950147746 1103 2 0.005323071492377096 1103 3 -0.21123163459733263 1103 4 0.23031426636213936 1103 5 0.006732354328764956 1103 6 -0.4546606551372392 1103 7 0.04360160440140248 1103 8 0.029421533387868237 1103 9 -0.19145281451555718 1103 10-11 -0.544951994746846 1103 12-13 0.04909295304241823 1103 14-15 -0.12995902225367928 1103 16-17 0.055279701941230996 1103 18-19 0.07825109325064972 1103 20-21 0.035195675497526224 1103 22-23 -0.24197217889754796 1103 24-25 -0.03688265882926345 1103 26-27 -0.10918920997789172 1103 28-29 -0.008788836312120907 1103 30-31 -0.34169952738375997 1103 32-33 -0.048547440241293316 1103 34-35 -0.16117722873385532 1103 36-37 0.11639962989526964 1103 38-39 -0.2754889551550619 1103 40-41 0.12427881971767363 1103 42-43 -0.003886467726125886 1103 44-45 0.15208673400493922 1103 46-47 0.06987706665809412 1103 48-49 -0.1652411163416332 1103 50-51 -0.245234531091711 1103 52-53 0.02140736649403152 1103 54-55 -0.05794940885687083 1103 56-57 0.05897392559368342 1103 58-59 -0.33979675679611887 1103 60-61 0.18177766227027803 1103 62-63 -0.36989968554623687 1103 64-65 0.21196277448790113 1103 66-67 -0.2488653160388168 1103 68-69 0.13666077166983115 1103 70-71 -0.3408163690922308 1103 72-73 -0.10375141256776033 1103 74-75 -0.16540000294845214 1103 76 0.18683220723194438 1104 1 0.11829252354844755 1104 2 -0.09663673996879751 1104 3 -0.20607516863709563 1104 4 0.2893566875975644 1104 5 -0.39457418664535027 1104 6 0.20552646610173042 1104 7 0.08924784602879043 1104 8 -0.2512248854376651 1104 9 -0.17913163697483014 1104 10-11 -0.1348800523008009 1104 12-13 0.06515005809728791 1104 14-15 -0.07846228860611859 1104 16-17 -0.16722800617798583 1104 18-19 -0.38170803304437584 1104 20-21 -0.30961714419746045 1104 22-23 -0.5348866158209162 1104 24-25 -0.4113879806801535 1104 26-27 -0.33731114592666245 1104 28-29 -0.14366320282876188 1104 30-31 -0.1905199784120981 1104 32-33 -0.11720876733725305 1104 34-35 -0.14564876480549316 1104 36-37 -0.03667889250848333 1104 38-39 -0.1632014452614925 1104 40-41 -0.4428172571154434 1104 42-43 -0.1855204708684397 1104 44-45 -0.049591332408922995 1104 46-47 -0.07519209968370433 1104 48-49 -0.34539846829258636 1104 50-51 -0.6069845399516112 1104 52-53 -0.7228396004644182 1104 54-55 -0.2504131718366267 1104 56-57 -0.14116547027877857 1104 58-59 -0.1298317238963307 1104 60-61 0.12635303645369333 1104 62-63 -0.16619270039543466 1104 64-65 0.04533745405459655 1104 66-67 -0.35093439968814977 1104 68-69 -0.0933335579895882 1104 70-71 -0.18034868412690486 1104 72-73 -0.11616709578473206 1104 74-75 -0.3118306416886938 1104 76 -0.20876264693398383 1105 1 0.07888365655337282 1105 2 0.05114651126272918 1105 3 0.03776719589492217 1105 4 -0.006209814865488994 1105 5 0.011829754241347246 1105 6 0.5010929685647909 1105 7 0.7197897179499719 1105 8 -0.04432833371352274 1105 9 0.39229693445373925 1105 10-11 0.11142536641841616 1105 12-13 -0.412682454217979 1105 14-15 0.3932125882411768 1105 16-17 0.21085081155599994 1105 18-19 -0.013481432059151643 1105 20-21 0.16328925974342923 1105 22-23 0.30747791619879195 1105 24-25 0.09969576316220952 1105 26-27 0.3942159476693945 1105 28-29 -0.15105236539033484 1105 30-31 0.18263273094750332 1105 32-33 0.1032345824164409 1105 34-35 0.02430197411076307 1105 36-37 -0.08840303043951536 1105 38-39 0.35403993404884915 1105 40-41 0.31457190544612956 1105 42-43 0.24797706607736814 1105 44-45 -0.05205438659611872 1105 46-47 0.27825617617835974 1105 48-49 0.12381335436751328 1105 50-51 0.05927161768385503 1105 52-53 -0.014711521646688652 1105 54-55 0.2717543158480993 1105 56-57 -0.1325447806236042 1105 58-59 0.12016827610366931 1105 60-61 -0.06330213596010736 1105 62-63 0.02099941783115611 1105 64-65 -0.04949013215230735 1105 66-67 0.0025138761739214033 1105 68-69 0.15420338782321608 1105 70-71 0.25068579863172147 1105 72-73 0.1178230519985135 1105 74-75 0.044080688360573106 1105 76 -0.15457545481575607 1106 1 -0.048676623456998414 1106 2 -0.018514317926232593 1106 3 0.10323258350187103 1106 4 0.412356192629737 1106 5 -0.18619273143994164 1106 6 0.13537066908568107 1106 7 -0.15072905547260973 1106 8 0.3639022741541069 1106 9 -0.0050679511144124945 1106 10-11 0.10674674195757206 1106 12-13 0.10476516168698424 1106 14-15 0.23728593775916806 1106 16-17 -0.041971683553242656 1106 18-19 0.40540276903920613 1106 20-21 0.2523540062491918 1106 22-23 0.13502287577642136 1106 24-25 0.10873481848074817 1106 26-27 -0.19420062668184812 1106 28-29 0.22528228000057027 1106 30-31 0.17289836368138367 1106 32-33 -0.13393750139810834 1106 34-35 -0.14221345238652106 1106 36-37 0.1025131314385348 1106 38-39 0.0023299380557304517 1106 40-41 0.4631742105820109 1106 42-43 0.051734107583953914 1106 44-45 -0.04052281471972208 1106 46-47 -0.08292891821907489 1106 48-49 -0.07440947543426546 1106 50-51 0.24538541314429096 1106 52-53 0.3006949412189428 1106 54-55 0.0851867998794944 1106 56-57 -0.01219688895085369 1106 58-59 -0.2054439519586424 1106 60-61 -0.1278129427576573 1106 62-63 -0.17562065900670376 1106 64-65 0.21721503201202808 1106 66-67 -0.16699492718242936 1106 68-69 0.13750411613302305 1106 70-71 0.025940942761636165 1106 72-73 0.1786628474118146 1106 74-75 -0.3002519300192361 1106 76 0.323763810274869 1107 1 0.01665278397767267 1107 2 -0.515815262595595 1107 3 -0.22206492600616556 1107 4 -0.291545169166362 1107 5 -0.09982296085964393 1107 6 -0.77960908799934 1107 7 0.2912412549187522 1107 8 -0.006113674809213876 1107 9 0.12534549508851 1107 10-11 0.1460057285849814 1107 12-13 0.5764864228819278 1107 14-15 0.10104498572874832 1107 16-17 0.2060653671153858 1107 18-19 0.2029429481583307 1107 20-21 0.1838348061510473 1107 22-23 0.44452477738358454 1107 24-25 0.15173567727108406 1107 26-27 0.34554909727840766 1107 28-29 0.18725013670527346 1107 30-31 0.1401723177974361 1107 32-33 0.1558428936454419 1107 34-35 0.48945750823181555 1107 36-37 0.3782768414127702 1107 38-39 -0.15068809654125204 1107 40-41 0.41658483504527055 1107 42-43 0.2863065526827171 1107 44-45 -0.046350320547219326 1107 46-47 0.14336233438797308 1107 48-49 0.08676706732123307 1107 50-51 0.3129231385767568 1107 52-53 0.06081639870882327 1107 54-55 0.2333588033146583 1107 56-57 0.11376925017317774 1107 58-59 0.18797403356460052 1107 60-61 0.31020079788765287 1107 62-63 0.10796417720971618 1107 64-65 0.2628842750496929 1107 66-67 0.43566118863158465 1107 68-69 0.05561232371491087 1107 70-71 -0.3742553521715024 1107 72-73 0.29272860621441055 1107 74-75 0.26020077517031126 1107 76 0.4335662884984046 1108 1 -0.730970951510141 1108 2 -0.3761814336413778 1108 3 -0.435091381751068 1108 4 0.20005303845126576 1108 5 0.04851252242729487 1108 6 0.05221927780961266 1108 7 -0.43175985400468875 1108 8 -0.34209484574184046 1108 9 -0.2825799128369013 1108 10-11 -0.10454024647555826 1108 12-13 -0.6071082995477397 1108 14-15 -0.22593466892081437 1108 16-17 -0.10672176484650464 1108 18-19 -0.4915231844145964 1108 20-21 -0.4984473130242719 1108 22-23 -0.2878936941125545 1108 24-25 -0.8447480170281452 1108 26-27 -0.8502720718437331 1108 28-29 -0.6003146447915526 1108 30-31 -0.4752179553598239 1108 32-33 -0.5073697028195809 1108 34-35 -0.46854201651528626 1108 36-37 -0.6092732298751642 1108 38-39 -0.3340847969282379 1108 40-41 -0.42618566306504135 1108 42-43 -0.2981753084981733 1108 44-45 -0.2883162833869761 1108 46-47 -0.3370051945232504 1108 48-49 -0.629654052012512 1108 50-51 -0.5835735685995616 1108 52-53 -0.2934252334454328 1108 54-55 -0.5085675551165565 1108 56-57 -0.4877566511100042 1108 58-59 -0.6317806454133787 1108 60-61 -0.183405919020025 1108 62-63 -0.2389364949336752 1108 64-65 -0.2455062017873857 1108 66-67 -0.08165442455782568 1108 68-69 -0.07271444660215565 1108 70-71 -0.002279431651892594 1108 72-73 -0.7402703527554166 1108 74-75 -0.4388453243612247 1108 76 -0.06604898296152584 1109 1 -0.28679619422858593 1109 2 0.26459526538774725 1109 3 -0.03703312932388414 1109 4 -0.12518967999533714 1109 5 -0.1238175746600838 1109 6 0.3750348117901936 1109 7 0.10464791298560527 1109 8 -0.6042307680719361 1109 9 0.10576960172620886 1109 10-11 0.13939179235939037 1109 12-13 9.014091901136112E-4 1109 14-15 0.07382261263258272 1109 16-17 0.18332677884281878 1109 18-19 0.3410010874300582 1109 20-21 -0.046991002344668686 1109 22-23 -0.0342529174135251 1109 24-25 -0.12872859955241012 1109 26-27 0.30142695728247304 1109 28-29 -0.3041981399371778 1109 30-31 -0.09900436312680938 1109 32-33 -0.03164154747977932 1109 34-35 0.19893371164005913 1109 36-37 0.01329958565881384 1109 38-39 0.5566919021008871 1109 40-41 -0.059680808696100485 1109 42-43 0.08531983713290003 1109 44-45 0.13644099816642097 1109 46-47 0.44697538800102166 1109 48-49 -0.08717832385717372 1109 50-51 0.21375652848781357 1109 52-53 -0.01551261208621213 1109 54-55 0.15769458080576726 1109 56-57 0.06806859160844425 1109 58-59 0.16069508274195954 1109 60-61 0.003487284863467721 1109 62-63 0.30475282545467053 1109 64-65 0.2399306914164967 1109 66-67 0.14164654631596818 1109 68-69 0.38723700970852803 1109 70-71 0.1469924130083058 1109 72-73 -0.06429947896900501 1109 74-75 0.07329059796887094 1109 76 -0.034495584903275756 1110 1 0.3383640915380397 1110 2 0.4304452379101882 1110 3 -0.12603752573348004 1110 4 -0.08889640283834765 1110 5 -0.475576138499612 1110 6 0.051074379476581555 1110 7 -0.4672746002952195 1110 8 -0.7047986376451192 1110 9 0.2244955588612143 1110 10-11 -0.1466725051309936 1110 12-13 -6.785505094555333E-4 1110 14-15 -0.5343015859385787 1110 16-17 0.005615659590212374 1110 18-19 -0.04616918185002561 1110 20-21 0.09747226931681041 1110 22-23 -0.3361053629163635 1110 24-25 -0.05910733192097695 1110 26-27 -0.32320667525095814 1110 28-29 0.1383623106948022 1110 30-31 -0.014545213099339094 1110 32-33 0.10274661885105729 1110 34-35 -0.06686588535573179 1110 36-37 0.06891874667731912 1110 38-39 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0.772755415679498 2116 12-13 0.38904168371305303 2116 14-15 0.6173505192756608 2116 16-17 0.490407461802306 2116 18-19 0.6995727551329622 2116 20-21 0.2544222646695431 2116 22-23 0.50944835954855 2116 24-25 0.19821793064989635 2116 26-27 0.06539821367924503 2116 28-29 0.11865206810720252 2116 30-31 0.3821401201100656 2116 32-33 0.22515576468245513 2116 34-35 0.2792280824851403 2116 36-37 0.16652307793486898 2116 38-39 0.493202495625205 2116 40-41 0.08673939313086265 2116 42-43 0.37975046509215105 2116 44-45 0.472576155275803 2116 46-47 0.3915566687891996 2116 48-49 0.3738133543675133 2116 50-51 0.23045388369371267 2116 52-53 0.5210027640676032 2116 54-55 0.5045129365377505 2116 56-57 0.504154726765556 2116 58-59 0.6226313302908579 2116 60-61 0.18546633694629833 2116 62-63 0.46558069861934115 2116 64-65 0.03179065602503783 2116 66-67 0.218031117553231 2116 68-69 0.27119846171483886 2116 70-71 0.28516855725240475 2116 72-73 0.41831566283595123 2116 74-75 0.2817654174246087 2116 76 0.6730107520807991 2117 1 -0.3506816359883267 2117 2 -0.49504655761636585 2117 3 -0.2915042586033927 2117 4 -0.5859349898719692 2117 5 -0.716153998243314 2117 6 0.05813211816292352 2117 7 0.008874498867783132 2117 8 -0.3130172975023058 2117 9 -0.6810761534384042 2117 10-11 -0.12485498964416308 2117 12-13 -0.38919701648571703 2117 14-15 -0.13910959653109956 2117 16-17 -0.11710269289478248 2117 18-19 -0.2888897805164987 2117 20-21 -0.3041508923609655 2117 22-23 -0.22445536528076815 2117 24-25 -0.35800020088584716 2117 26-27 -0.12892379824712208 2117 28-29 -0.16455595194292272 2117 30-31 -0.16333995939905321 2117 32-33 -0.31678097371526803 2117 34-35 -0.3358224749429084 2117 36-37 -0.21447251449448146 2117 38-39 -0.23240462562619513 2117 40-41 -0.33900168369914496 2117 42-43 -0.33343335653087536 2117 44-45 -0.2872760614729657 2117 46-47 -0.09876396036990798 2117 48-49 -0.26152499149714714 2117 50-51 0.11836331243797815 2117 52-53 -0.3090681027546296 2117 54-55 -0.33854297911344133 2117 56-57 -0.35333382219305065 2117 58-59 -0.2868406254605844 2117 60-61 -0.1581729336439608 2117 62-63 -0.09860995041704967 2117 64-65 -0.3081324275459636 2117 66-67 -0.13481219763127683 2117 68-69 -0.3090668567537449 2117 70-71 -0.28062091643180054 2117 72-73 -1.7789015180472916 2117 74-75 -0.6262373481099175 2117 76 -1.1371612295975382 2118 1 0.021178671715240682 2118 2 -0.06332857653226753 2118 3 0.31996968172187223 2118 4 -0.7540194345603339 2118 5 -0.28315544346219923 2118 6 -0.018647421928186247 2118 7 0.2902714218321307 2118 8 0.37521788900410513 2118 9 0.17484101173730693 2118 10-11 -0.6385296873372965 2118 12-13 -0.26143234934341564 2118 14-15 -0.23957060306202038 2118 16-17 0.01977234140630202 2118 18-19 0.20356398162607547 2118 20-21 0.15895943820999037 2118 22-23 0.32492904464916705 2118 24-25 0.034385873789894106 2118 26-27 0.07988289000635973 2118 28-29 0.37794994784303526 2118 30-31 0.37700725901193977 2118 32-33 -0.07979251717245006 2118 34-35 -0.11023112430353166 2118 36-37 -0.13949492453418344 2118 38-39 0.017041983597586352 2118 40-41 -0.4364259011771452 2118 42-43 0.2880930873159713 2118 44-45 0.05978685128337702 2118 46-47 -0.36506840761941817 2118 48-49 -0.07961035088100488 2118 50-51 0.06626225687937648 2118 52-53 -0.023491021365018128 2118 54-55 -0.001953329807697912 2118 56-57 0.0431500883131406 2118 58-59 0.10799683314944275 2118 60-61 0.030812303170698385 2118 62-63 0.011482799706435287 2118 64-65 0.10570325655480417 2118 66-67 -0.19614253076338173 2118 68-69 0.16071160399667406 2118 70-71 0.017267966359113984 2118 72-73 0.20818298152281045 2118 74-75 -0.12109675941189124 2118 76 0.07144422564640962 2119 1 0.2759697382098807 2119 2 0.03645167950185524 2119 3 -0.19375989770113478 2119 4 -0.4897198758156449 2119 5 0.26477289526163617 2119 6 0.27770647779080093 2119 7 0.045831297644390645 2119 8 0.21283122210179073 2119 9 0.09998667311951692 2119 10-11 0.33727491137958765 2119 12-13 -0.12423376088910487 2119 14-15 0.23436646650785065 2119 16-17 0.1511739308680191 2119 18-19 -0.2655687661160897 2119 20-21 -0.25436541171018945 2119 22-23 -0.12898363510787902 2119 24-25 0.19684029017231097 2119 26-27 0.2882419975984192 2119 28-29 0.1743421407464325 2119 30-31 0.12462571750506868 2119 32-33 -0.029728597010787894 2119 34-35 0.22209774934663074 2119 36-37 0.13750913454337166 2119 38-39 -0.03581144776629941 2119 40-41 0.39877496130318946 2119 42-43 0.059073333924075655 2119 44-45 -0.0778329621993521 2119 46-47 -0.30302461199897834 2119 48-49 0.06872860860480046 2119 50-51 -0.037768542623396684 2119 52-53 0.06869834726873592 2119 54-55 -0.13742577933433608 2119 56-57 -0.03546287363521117 2119 58-59 0.12354975727591011 2119 60-61 0.11673042645118414 2119 62-63 0.01791016342688323 2119 64-65 -0.19913111069450906 2119 66-67 -0.24011783460507274 2119 68-69 -0.5011360126944382 2119 70-71 -0.282022726040573 2119 72-73 -0.08001446991849548 2119 74-75 -0.2078396589718139 2119 76 0.005356080627180404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 12.0 13 234.0 14 978.0 15 1875.0 16 2431.0 17 2851.0 18 2931.0 19 2577.0 20 1971.0 21 1476.0 22 1098.0 23 928.0 24 860.0 25 893.0 26 974.0 27 1103.0 28 1377.0 29 1655.0 30 2049.0 31 2658.0 32 3386.0 33 4789.0 34 6514.0 35 10095.0 36 19877.0 37 80747.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.3542238341041 19.357933720952545 13.69140137777522 27.596441067168143 2 21.540370604903448 19.655364304492164 32.155764076781864 26.64850101382253 3 19.56453604027146 22.875290234682325 27.59708070283167 29.963093022214547 4 15.6032723760546 18.20914806925975 30.663494073775578 35.524085480910074 5 17.32517158226674 31.56026327403911 31.482867358752454 19.6316977849417 6 30.01874132494131 33.35891875987437 17.094902743397363 19.52743717178695 7 28.28404940545865 27.685990060061787 20.296279239345267 23.73368129513429 8 25.03470023474629 32.41097870652876 18.850702639776383 23.703618418948565 9 24.117462693249927 17.762042740455037 19.89586731397796 38.22462725231708 10-11 27.300609572787337 23.24244110554628 23.81587447789739 25.64107484376899 12-13 25.830407000172702 21.019707174793236 23.55298422018818 29.596901604845883 14-15 24.882786764658853 21.81957157203257 21.80645904093029 31.491182622378293 16-17 25.77635778660475 23.61726760437255 22.64342230665413 27.96295230236857 18-19 24.8901425747894 22.74992164463122 23.739438016106025 28.620497764473356 20-21 24.751021817972482 23.48230447936855 23.760226175170622 28.006447527488344 22-23 26.39776383372031 22.949168153819585 22.978911212173546 27.67415680028656 24-25 25.565597835472914 23.66076282949232 22.559949852563978 28.213689482470784 26-27 24.48973064942209 23.866405695315947 23.91118019176277 27.732683463499193 28-29 25.32709473354292 23.238677612999997 24.76325409288179 26.67097356057529 30-31 25.04013713788626 22.813885210983823 24.598468712221518 27.5475089389084 32-33 24.40945637364957 23.734320930797818 24.36756023768861 27.488662457864 34-35 24.85944006294015 23.528997882805953 24.478856843142147 27.13270521111175 36-37 25.161028278292687 22.579458740301526 24.542180773831227 27.717332207574565 38-39 23.956274506041357 22.73521002437012 26.281989778622094 27.026525690966423 40-41 24.159678647042647 22.230217668016298 26.321967007592473 27.288136677348586 42-43 23.159042718204407 21.97283458649021 26.49251463970807 28.37560805559731 44-45 22.40778822874724 21.99746063829447 27.482993632406604 28.111757500551686 46-47 22.537242786508806 22.545877867966407 27.432054701641945 27.484824643882845 48-49 22.174889183121294 22.74256583450067 27.4739508376029 27.608594144775132 50-51 21.907841293599166 23.700420240630937 26.477078655997545 27.914659809772353 52-53 21.25221473848496 23.753190182871837 26.500425357716246 28.49416972092696 54-55 21.21774227077783 23.4097807002114 26.509145220147307 28.863331808863457 56-57 21.35167808416326 23.639974670427723 26.2906248600797 28.717722385329314 58-59 21.807418494425576 23.50245300276962 25.725186933522664 28.96494156928214 60-61 21.73418021095184 22.66676900837283 26.022937334894042 29.57611344578128 62-63 21.86914333595584 22.946929428997244 25.688407882869917 29.495519352177002 64-65 22.257082365884393 23.19670715560417 24.739828193860777 29.806382284650663 66-67 22.219024043904593 23.680911352893393 23.89646857150167 30.203596031700343 68-69 21.93381668622892 24.55025473571769 23.798040789696714 29.717887788356673 70-71 22.28010924977133 24.995682459271197 23.657884469006454 29.066323821951016 72-73 21.901764754795668 25.239703464906388 23.723127306686113 29.135404473611832 74-75 21.76559196870892 26.386974417689817 23.807315536480136 28.040118077121118 76 22.362445470710895 26.355076820734563 22.845373485652882 28.43710422290166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.5 12 3.0 13 1.5 14 2.0 15 5.5 16 9.5 17 12.0 18 14.5 19 24.5 20 31.5 21 31.0 22 57.0 23 100.0 24 156.5 25 196.0 26 282.0 27 420.0 28 569.5 29 667.0 30 822.5 31 1129.0 32 1590.5 33 1901.0 34 2198.5 35 2779.5 36 3300.0 37 3537.0 38 4067.0 39 5132.5 40 6176.5 41 6935.0 42 7185.0 43 7458.0 44 8016.0 45 8301.5 46 8302.0 47 8348.0 48 8173.5 49 7478.5 50 7004.0 51 6472.5 52 5294.0 53 4312.5 54 3978.0 55 3914.5 56 3529.0 57 3086.5 58 2966.0 59 2886.0 60 2761.5 61 2762.0 62 2807.0 63 2843.0 64 2876.0 65 2983.5 66 3122.5 67 3151.0 68 3251.0 69 3347.5 70 3281.5 71 3219.0 72 3223.0 73 3107.0 74 2755.5 75 2524.0 76 2286.5 77 1787.0 78 1235.5 79 946.0 80 782.5 81 452.5 82 197.0 83 108.0 84 72.5 85 25.0 86 11.5 87 10.0 88 10.0 89 9.5 90 6.5 91 5.5 92 7.0 93 4.5 94 3.0 95 6.0 96 8.0 97 7.0 98 5.0 99 21.5 100 39.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.198178317630278E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 3.198178317630278E-4 44-45 3.198178317630278E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 3.198178317630278E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.198178317630278E-4 70-71 0.0 72-73 0.0 74-75 3.198178317630278E-4 76 6.396356635260555E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 156339.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.21132283051574 #Duplication Level Percentage of deduplicated Percentage of total 1 73.37237794894898 42.711031796288836 2 14.048369905611658 16.35548391636124 3 5.141362752315756 8.978565808915242 4 2.194336699374773 5.109409680246132 5 1.2768248596261826 3.7162832050863828 6 0.8966343248321558 3.1316562086235677 7 0.6702781104750184 2.7312442832562573 8 0.4966650916962432 2.3129225593102167 9 0.4285384640741921 2.245121178976455 >10 1.4735130264704914 12.675659944095843 >50 0.0010988165745492106 0.03262141883982884 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0057567209717345 0.0 2 0.0 0.0 0.0 0.008954899289364777 0.0 3 0.0 0.0 0.0 0.009594534952890834 0.0 4 0.0 0.0 0.0 0.040936682465667555 0.0 5 0.0 0.0 0.0 0.04861231042798022 0.0 6 0.0 0.0 0.0 0.04989158175503233 0.0 7 0.0 0.0 0.0 0.04989158175503233 0.0 8 0.0 0.0 0.0 0.0518104887456105 0.0 9 0.0 0.0 0.0 0.056927574053818944 0.0 10 0.0 0.0 0.0 0.060765388034975276 0.0 11 0.0 0.0 0.0 0.060765388034975276 0.0 12 0.0 0.0 0.0 0.061405023698501336 0.0 13 0.0 0.0 0.0 0.06204465936202739 0.0 14 0.0 0.0 0.0 0.06204465936202739 0.0 15 0.0 0.0 0.0 0.06268429502555345 0.0 16 0.0 0.0 0.0 0.06460320201613161 0.0 17 0.0 0.0 0.0 0.06652210900670978 0.0 18 0.0 0.0 0.0 0.06972028732434006 0.0 19 0.0 0.0 0.0 0.07739591528665273 0.0 20 0.0 0.0 0.0 0.080594093604283 0.0 21 0.0 0.0 0.0 0.08187336493133511 0.0 22 0.0 0.0 0.0 0.08763008590306962 0.0 23 0.0 0.0 0.0 0.09722462085596044 0.0 24 0.0 0.0 0.0 0.10298134182769494 0.0 25 0.0 0.0 0.0 0.11129660545353366 0.0 26 0.0 0.0 0.0 0.12281004739700267 0.0 27 0.0 0.0 0.0 0.2245121178976455 0.0 28 0.0 0.0 0.0 0.29870985486666796 0.0 29 0.0 0.0 0.0 0.39209666174147206 0.0 30 0.0 0.0 0.0 0.4816456546351198 0.0 31 0.0 0.0 0.0 0.6038160663685964 0.0 32 0.0 0.0 0.0 0.7349413773914378 0.0 33 0.0 0.0 0.0 0.9178771771598897 0.0 34 0.0 0.0 0.0 1.1455874733751654 0.0 35 0.0 0.0 0.0 1.495468181323918 0.0 36 0.0 0.0 0.0 1.95728513038973 0.0 37 0.0 0.0 0.0 2.580929902327634 0.0 38 0.0 0.0 0.0 3.266619333627566 0.0 39 0.0 0.0 0.0 3.936317873339346 0.0 40 0.0 0.0 0.0 4.6469530955167935 0.0 41 0.0 0.0 0.0 5.521974683220438 0.0 42 0.0 0.0 0.0 6.3778072010183 0.0 43 0.0 0.0 0.0 7.282891664907669 0.0 44 0.0 0.0 0.0 8.111859484837437 0.0 45 0.0 0.0 0.0 8.972169452279982 0.0 46 0.0 0.0 0.0 9.827362334414318 0.0 47 0.0 0.0 0.0 10.667203960624029 0.0 48 0.0 0.0 0.0 11.4226136792483 0.0 49 0.0 0.0 0.0 12.10190675391297 0.0 50 0.0 0.0 0.0 12.827893232015045 0.0 51 0.0 0.0 0.0 13.498871043053876 0.0 52 0.0 0.0 0.0 14.244046591061732 0.0 53 0.0 0.0 0.0 14.989861774733113 0.0 54 0.0 0.0 0.0 15.745271493357384 0.0 55 0.0 0.0 0.0 16.43351946731142 0.0 56 0.0 0.0 0.0 17.055245332258746 0.0 57 0.0 0.0 0.0 17.708952980382374 0.0 58 0.0 0.0 0.0 18.312129411087444 0.0 59 0.0 0.0 0.0 18.880765515962107 0.0 60 0.0 0.0 0.0 19.4353296362392 0.0 61 0.0 0.0 0.0 20.10182999763335 0.0 62 0.0 0.0 0.0 20.69988934303021 0.0 63 0.0 0.0 0.0 21.328651200276322 0.0 64 0.0 0.0 0.0 21.957413057522434 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGCG 15 0.0022212998 70.0 15 AACGGGG 20 0.0069408608 52.5 11 GGGACGT 20 0.0069408608 52.5 54 CTCGGTT 20 0.0069408608 52.5 44 TAGATCT 50 3.461555E-6 42.000004 39 GTACATG 40 0.0024130652 35.0 1 TTGTGAT 40 0.0024130652 35.0 10 AAGAGAC 45 0.0042971913 31.111109 5 GCGAACT 45 0.0042971913 31.111109 28 AATAGAG 45 0.0042971913 31.111109 5 TTCAACA 50 0.0071916208 28.000002 62 GTATAGG 50 0.0071916208 28.000002 1 CGGTCGT 50 0.0071916208 28.000002 10 TCAAGCC 50 0.0071916208 28.000002 21 GTAGATC 75 5.557688E-5 27.999998 38 TAATACA 65 7.9284416E-4 26.923077 4 TCTACAC 65 7.9284416E-4 26.923077 3 AGATCTC 80 8.623729E-5 26.25 40 ACATGCT 75 0.0018259877 23.333332 3 GGCATCA 80 0.0026569501 21.875 19 >>END_MODULE