FastQCFastQC Report
Wed 25 May 2016
SRR1780234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156339
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT82725.291066208687532No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC24801.5862964455446178No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG21401.368820319945759No Hit
CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG21321.3637032346375504RNA PCR Primer, Index 15 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5110.3268538240618144No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT2340.149674745265097RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT1910.12217041173347662No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGAG207.907238E-570.055
GTCAGTG207.907238E-570.051
TTGCGAT150.002221299870.049
ATGCCAT150.002221299870.043
AACAGGA150.002221299870.02
GCTATAC252.3846285E-456.0000043
CAGTGGT252.3846285E-456.00000453
TCCATAG200.006940860852.528
TGCCATG200.006940860852.519
CGTACTG200.006940860852.55
GCTAGTC200.006940860852.57
AGCTAGT200.006940860852.56
CGGTAAG200.006940860852.528
CAGAGAC200.006940860852.57
GTCGGTT200.006940860852.532
CTTTATA750.051.333332
AAAGTCA305.86374E-446.66666443
GCATTAG305.86374E-446.66666434
CAAATTA305.86374E-446.66666424
GATTCAA305.86374E-446.66666420
Produced by FastQC (version 0.11.5)