##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780234_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 156339 Sequences flagged as poor quality 0 Sequence length 76 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.74671067360032 34.0 34.0 34.0 34.0 34.0 2 33.74227160209545 34.0 34.0 34.0 34.0 34.0 3 33.766596946379345 34.0 34.0 34.0 34.0 34.0 4 33.79774720319306 34.0 34.0 34.0 34.0 34.0 5 33.793237771765206 34.0 34.0 34.0 34.0 34.0 6 37.566071165863924 38.0 38.0 38.0 37.0 38.0 7 37.58160791613097 38.0 38.0 38.0 37.0 38.0 8 37.487523906382926 38.0 38.0 38.0 37.0 38.0 9 37.50659784186927 38.0 38.0 38.0 37.0 38.0 10-11 37.44994850932909 38.0 38.0 38.0 37.0 38.0 12-13 37.43878366882224 38.0 38.0 38.0 37.0 38.0 14-15 37.37971651347392 38.0 38.0 38.0 37.0 38.0 16-17 37.40972502062825 38.0 38.0 38.0 37.0 38.0 18-19 37.379032103313946 38.0 38.0 38.0 36.5 38.0 20-21 37.39802288616404 38.0 38.0 38.0 37.0 38.0 22-23 37.37782319190988 38.0 38.0 38.0 37.0 38.0 24-25 37.401697593051 38.0 38.0 38.0 37.0 38.0 26-27 37.318522569544385 38.0 38.0 38.0 36.5 38.0 28-29 37.284538726741246 38.0 38.0 38.0 36.0 38.0 30-31 37.28975175739899 38.0 38.0 38.0 36.5 38.0 32-33 37.246410044838456 38.0 38.0 38.0 36.0 38.0 34-35 37.24734711108553 38.0 38.0 38.0 36.0 38.0 36-37 37.248057106672036 38.0 38.0 38.0 36.0 38.0 38-39 36.84859184208675 38.0 38.0 38.0 35.0 38.0 40-41 37.02478908013995 38.0 38.0 38.0 36.0 38.0 42-43 37.18859017903402 38.0 38.0 38.0 36.0 38.0 44-45 37.241260977747075 38.0 38.0 38.0 36.0 38.0 46-47 37.1981815158086 38.0 38.0 38.0 36.0 38.0 48-49 37.21727464036485 38.0 38.0 38.0 36.0 38.0 50-51 37.10143981987859 38.0 38.0 38.0 36.0 38.0 52-53 37.10949922923903 38.0 38.0 38.0 36.0 38.0 54-55 37.07658677617229 38.0 38.0 38.0 36.0 38.0 56-57 37.11288929825571 38.0 38.0 38.0 36.0 38.0 58-59 37.03270776965441 38.0 38.0 38.0 36.0 38.0 60-61 37.04240784449178 38.0 38.0 38.0 36.0 38.0 62-63 37.01092177895471 38.0 38.0 38.0 36.0 38.0 64-65 36.93645219682868 38.0 38.0 38.0 35.5 38.0 66-67 36.677818714460244 38.0 38.0 38.0 34.5 38.0 68-69 36.604916239709866 38.0 38.0 38.0 34.0 38.0 70-71 36.04429476969918 38.0 37.5 38.0 32.5 38.0 72-73 35.13840116669545 38.0 37.0 38.0 25.5 38.0 74-75 34.8477859011507 38.0 37.0 38.0 23.0 38.0 76 34.21383020231676 38.0 35.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09333016522524673 1101 2 -0.06611272950402736 1101 3 -0.08677599764733657 1101 4 -0.019883887825322688 1101 5 -0.10831297392699923 1101 6 -0.20175648860409723 1101 7 -0.014961879890982743 1101 8 -0.24644214723883806 1101 9 -0.15454560593731514 1101 10-11 -0.06726023038016393 1101 12-13 0.15565114408413194 1101 14-15 0.19885917735964398 1101 16-17 0.1413760384978744 1101 18-19 0.12068950502107612 1101 20-21 0.1086524068384378 1101 22-23 0.006726950216147998 1101 24-25 -0.0775430082649109 1101 26-27 0.01622200794275841 1101 28-29 -0.15266827727807453 1101 30-31 -0.06865002025428879 1101 32-33 0.08196408742789174 1101 34-35 0.15169842448101178 1101 36-37 0.16405753204112727 1101 38-39 0.19433993879641065 1101 40-41 0.2259123662559901 1101 42-43 0.022948422598609852 1101 44-45 -9.611193244225547E-4 1101 46-47 0.0524244823474973 1101 48-49 -0.06349402696712758 1101 50-51 0.1518550827170273 1101 52-53 0.23205021424012529 1101 54-55 -0.10397998611550463 1101 56-57 0.1233629736229176 1101 58-59 0.20841228438652593 1101 60-61 -0.10534800595979021 1101 62-63 0.1063191265356096 1101 64-65 -0.14464101103190075 1101 66-67 -0.2601531795106595 1101 68-69 -0.4252758933991956 1101 70-71 -0.38434435488798613 1101 72-73 -0.43701742015960576 1101 74-75 -0.07671210089169023 1101 76 -0.8913323859348026 1102 1 -0.0546828514708011 1102 2 -0.06453899394002605 1102 3 -0.09268355884143631 1102 4 -0.05987492963826213 1102 5 -0.09014843382095705 1102 6 0.031107952235551295 1102 7 -0.027582028548465587 1102 8 -0.23551073789811028 1102 9 0.015678106625941268 1102 10-11 -0.1140879426528798 1102 12-13 -0.10560711802757794 1102 14-15 0.053291664103944925 1102 16-17 0.12666161097445183 1102 18-19 0.006245638958077393 1102 20-21 -0.051907964078928615 1102 22-23 -0.03498006864447234 1102 24-25 0.03722165773997688 1102 26-27 0.05497827262086474 1102 28-29 0.01950747266744912 1102 30-31 -0.01173526829922622 1102 32-33 0.05419068113778991 1102 34-35 -0.05342892704309321 1102 36-37 -0.10798384509855197 1102 38-39 -0.02109840708644839 1102 40-41 -0.08545726791101771 1102 42-43 0.010340379599320215 1102 44-45 -0.011126845784978912 1102 46-47 0.0783669078371716 1102 48-49 0.12211295073424111 1102 50-51 -0.1540125298050654 1102 52-53 0.12658874655012653 1102 54-55 0.2012974755700867 1102 56-57 0.08164384910411115 1102 58-59 -0.02956074420704624 1102 60-61 0.09724018376008559 1102 62-63 0.28253513990026136 1102 64-65 -0.0437403500629614 1102 66-67 0.18635191697143227 1102 68-69 0.4627013721747346 1102 70-71 0.22078420720193748 1102 72-73 0.2026727960474446 1102 74-75 0.23213047712927448 1102 76 0.18838942604011777 1103 1 0.1673550521868563 1103 2 0.15284826130432094 1103 3 0.12710177793768196 1103 4 -0.04225601506118437 1103 5 0.03333530346106528 1103 6 -0.34781221260345063 1103 7 -0.0917423507761157 1103 8 -0.25514939590374297 1103 9 -0.08410420344833369 1103 10-11 -0.05225876521813433 1103 12-13 0.01618864924210328 1103 14-15 -0.06832657528512698 1103 16-17 -0.11837924954102164 1103 18-19 -0.08727081001866566 1103 20-21 -0.1544637835010647 1103 22-23 -0.18172577887240493 1103 24-25 -0.06412930032851705 1103 26-27 0.04652096080042156 1103 28-29 0.024131928442635342 1103 30-31 0.0435544804842678 1103 32-33 0.035183556740228994 1103 34-35 -0.2501327290500299 1103 36-37 0.06054831946106276 1103 38-39 0.14786739114874337 1103 40-41 -0.15070603976731434 1103 42-43 -0.0830276444280642 1103 44-45 -0.1350383961563253 1103 46-47 -0.026927497415321966 1103 48-49 -0.04179766834330678 1103 50-51 -0.05291476723841981 1103 52-53 0.019230943472202 1103 54-55 -0.14858669476354436 1103 56-57 -0.07905962694999147 1103 58-59 -0.0360215949439322 1103 60-61 0.1276934910171903 1103 62-63 0.0043169377629368455 1103 64-65 -0.1170331721160025 1103 66-67 -0.1237107125366137 1103 68-69 0.027644563252941623 1103 70-71 0.03063438531187046 1103 72-73 0.016620666876995926 1103 74-75 0.06594982941443561 1103 76 -0.058211351590209404 1104 1 0.024362057420105998 1104 2 -0.06941727601951442 1104 3 -0.013817998556390876 1104 4 0.022350427863294442 1104 5 -0.020668783909435717 1104 6 -0.06837074115007624 1104 7 0.14065892032522953 1104 8 -4.68411627124965E-4 1104 9 -0.11700399199187927 1104 10-11 -0.1213276480429073 1104 12-13 -0.09808643315503218 1104 14-15 -0.01282221030420061 1104 16-17 0.01636221836839269 1104 18-19 -0.060513890431181494 1104 20-21 -0.11319138270248885 1104 22-23 0.037757747606903536 1104 24-25 -0.1543645013302779 1104 26-27 -0.05725808492852025 1104 28-29 -0.02880306346531114 1104 30-31 -0.04318105719395504 1104 32-33 0.060868427562773775 1104 34-35 -0.022072812451284562 1104 36-37 -0.14156388240306228 1104 38-39 -0.41432620372904694 1104 40-41 -0.11094868309108108 1104 42-43 -0.09896661738762447 1104 44-45 -0.09264078714993929 1104 46-47 -0.13791555426048774 1104 48-49 0.020440226070228107 1104 50-51 -0.3465970627812993 1104 52-53 -0.34010621734101676 1104 54-55 -0.20958420217633744 1104 56-57 -0.36240536415333224 1104 58-59 -0.1829277153677893 1104 60-61 -0.1097520205538487 1104 62-63 -0.14401562771176657 1104 64-65 -0.368923285640939 1104 66-67 -0.9105561130634854 1104 68-69 -0.6198049648093402 1104 70-71 -0.6214547807936697 1104 72-73 -0.48969054031488213 1104 74-75 -0.34703997578574075 1104 76 -0.453977497294332 1105 1 -0.005194592826200051 1105 2 0.09560735452235747 1105 3 0.05761057287217852 1105 4 0.044517915548020426 1105 5 0.15174500919401623 1105 6 0.21734354456421556 1105 7 0.14065892032522953 1105 8 0.10790597260932344 1105 9 0.09974477648102464 1105 10-11 0.1409876228930571 1105 12-13 0.1026524831011244 1105 14-15 0.12880340545934388 1105 16-17 -0.01935206734589201 1105 18-19 0.09589005045552312 1105 20-21 0.1675967946374115 1105 22-23 0.19293016140000674 1105 24-25 0.05745815876824878 1105 26-27 0.03018033871680359 1105 28-29 0.03523634540167819 1105 30-31 0.13539037137746845 1105 32-33 0.08303591524749976 1105 34-35 0.024725217105356023 1105 36-37 0.17124399937033985 1105 38-39 0.13000877164041214 1105 40-41 0.09717939572664847 1105 42-43 0.1239397865532581 1105 44-45 0.05637399117518527 1105 46-47 0.10223222899074358 1105 48-49 0.08571116203081175 1105 50-51 0.006851213080771856 1105 52-53 0.1438839304422288 1105 54-55 0.005933039202972168 1105 56-57 0.09695424175799872 1105 58-59 0.3281560284745808 1105 60-61 0.22891793018506945 1105 62-63 0.30056565307640426 1105 64-65 0.04117523652654853 1105 66-67 -0.11252655641323628 1105 68-69 0.16098321253056014 1105 70-71 0.0041609827531274846 1105 72-73 0.16301881929103246 1105 74-75 0.034733423229035054 1105 76 0.31695846329679966 1106 1 0.0790677872619554 1106 2 0.03828536616588707 1106 3 0.01253314576208453 1106 4 0.07796359872147463 1106 5 0.17490243253292448 1106 6 0.11816141997373819 1106 7 0.04106432254551606 1106 8 0.06011800739849349 1106 9 0.13976646078582888 1106 10-11 0.02332669942450849 1106 12-13 0.06771011149813688 1106 14-15 0.07923002058650042 1106 16-17 0.07231126025209988 1106 18-19 0.07228479908257412 1106 20-21 0.12842951908175593 1106 22-23 0.11685832035539079 1106 24-25 0.07139386009051663 1106 26-27 0.11136095483389852 1106 28-29 0.2494454575236773 1106 30-31 -0.04578553076031966 1106 32-33 0.06803956858146876 1106 34-35 0.02377063629596421 1106 36-37 0.01195274615893993 1106 38-39 -0.030307772069967598 1106 40-41 0.10078558989545883 1106 42-43 0.1882797116009911 1106 44-45 0.09675511640204348 1106 46-47 0.1161915002095455 1106 48-49 0.12756540579068343 1106 50-51 0.15624900222447735 1106 52-53 0.20309151052094876 1106 54-55 0.27143613864436844 1106 56-57 0.140935668679532 1106 58-59 0.16246554835578308 1106 60-61 0.23848141570137216 1106 62-63 0.26143373236553913 1106 64-65 0.3678009701395766 1106 66-67 0.15487981217280122 1106 68-69 0.408867814317162 1106 70-71 0.14291107114024015 1106 72-73 0.0462123045716325 1106 74-75 0.2264922089079704 1106 76 0.28599940420706815 1107 1 -0.021103970804539074 1107 2 0.02779585567637355 1107 3 0.0742433653670389 1107 4 -0.009571673024325378 1107 5 -0.050346003241827475 1107 6 -0.07199929650276715 1107 7 0.013521689739718568 1107 8 -0.004843347036548096 1107 9 -0.02598007338175279 1107 10-11 0.024982937922850112 1107 12-13 0.08945590955972449 1107 14-15 0.10217199089689188 1107 16-17 -0.043778790048477845 1107 18-19 -0.11796793064384303 1107 20-21 0.001696602875149722 1107 22-23 0.039015110933235064 1107 24-25 -0.030832860557254094 1107 26-27 -0.014697197540044726 1107 28-29 0.024534403665256832 1107 30-31 -0.17003408921940633 1107 32-33 -0.03170279431878953 1107 34-35 -0.3039805598762797 1107 36-37 0.09025602183063341 1107 38-39 0.12402911910903214 1107 40-41 0.10566229214058609 1107 42-43 -0.042520190251543966 1107 44-45 -0.16959257099826885 1107 46-47 0.014762227727636912 1107 48-49 -0.1536270859281217 1107 50-51 -0.10381111309879287 1107 52-53 -0.10840401150088042 1107 54-55 -0.19442694563976914 1107 56-57 -0.35995379273624906 1107 58-59 -0.4052255324931906 1107 60-61 -0.3216952497384469 1107 62-63 -0.1537300512192985 1107 64-65 0.014500761576208276 1107 66-67 0.1383174271893708 1107 68-69 0.08525721439077216 1107 70-71 0.07767367695547733 1107 72-73 0.15515599246268152 1107 74-75 0.42324720829454776 1107 76 0.28808503787165307 1108 1 -0.11050809418924956 1108 2 -0.015995304619636386 1108 3 -0.012586471462796567 1108 4 -0.01133124031787247 1108 5 -0.08529406488892022 1108 6 -0.28442602834697084 1108 7 0.013680107373865269 1108 8 0.07759605821838989 1108 9 0.0812120872084634 1108 10-11 -0.09102969022570306 1108 12-13 -0.13594692385281348 1108 14-15 -0.14695105434265798 1108 16-17 -0.14032868028768775 1108 18-19 -0.15516212803220952 1108 20-21 -0.014286604855620055 1108 22-23 -0.11629074825611951 1108 24-25 -0.12904047818234687 1108 26-27 -0.2180799798063191 1108 28-29 0.008585142570360915 1108 30-31 -0.050570219755535106 1108 32-33 -0.27590640624084983 1108 34-35 -0.12503086879066672 1108 36-37 -0.1516731654868977 1108 38-39 -0.16308869834858797 1108 40-41 -0.14735047179450333 1108 42-43 -0.27364020292495894 1108 44-45 0.03013529013735905 1108 46-47 0.1281241893905687 1108 48-49 -0.08298413664439153 1108 50-51 -0.12367272397982276 1108 52-53 -0.2993821272362851 1108 54-55 -0.3387510681192296 1108 56-57 -0.24117168487646268 1108 58-59 -0.29048307430413445 1108 60-61 0.002579989585328235 1108 62-63 -0.12401825815798873 1108 64-65 -0.04980831122801277 1108 66-67 -0.1558810927373102 1108 68-69 -0.41472581229128735 1108 70-71 -0.3350410355165181 1108 72-73 -0.293686427253526 1108 74-75 -0.2460248626765562 1108 76 0.0573214648750664 1109 1 0.05211326503405189 1109 2 0.0908008118852166 1109 3 0.052947509119725567 1109 4 0.015367788808340777 1109 5 0.11130787685865329 1109 6 0.009263292041374882 1109 7 -0.08583445573292892 1109 8 -0.23551073789811028 1109 9 0.09092082507254418 1109 10-11 -0.20389376789560032 1109 12-13 -0.28521876851301187 1109 14-15 -0.11782484075042277 1109 16-17 -0.03353256378283476 1109 18-19 -0.05928834162444474 1109 20-21 0.02212116213466686 1109 22-23 -0.11143637932407557 1109 24-25 -0.12175892478428807 1109 26-27 0.06590060271795295 1109 28-29 0.008585142570360915 1109 30-31 0.07200259577845003 1109 32-33 0.0930256325940988 1109 34-35 -0.043720189179012436 1109 36-37 -0.09948869946748573 1109 38-39 -0.21284597990199217 1109 40-41 -0.11943785043528266 1109 42-43 0.033398632026496955 1109 44-45 0.10537800858396196 1109 46-47 0.12691059715756126 1109 48-49 0.0966275138410424 1109 50-51 -0.24745913174681533 1109 52-53 -0.004479214614917737 1109 54-55 -0.12637242734253817 1109 56-57 -0.11859886934249175 1109 58-59 -0.1921821034303406 1109 60-61 0.009861542983394145 1109 62-63 -0.12401825815798873 1109 64-65 -0.13475976753868935 1109 66-67 0.02130337328210885 1109 68-69 -0.11254134627186829 1109 70-71 0.06665799360970226 1109 72-73 -0.7293660389040113 1109 74-75 -0.19384039665713715 1109 76 0.05974864934108837 1110 1 -0.04332546000716064 1110 2 -0.26746077448591166 1110 3 -0.06447326391563024 1110 4 -0.028665001079922092 1110 5 0.048017897008868715 1110 6 -0.0047960594882070495 1110 7 -0.149307619316005 1110 8 -0.14082764575669415 1110 9 0.005625899261950451 1110 10-11 -0.21343077100971897 1110 12-13 -0.023311825849518186 1110 14-15 -0.2210374254729004 1110 16-17 -0.020595212619610948 1110 18-19 0.011454926373360763 1110 20-21 0.019464998002042933 1110 22-23 -0.05045909410701199 1110 24-25 0.03738194350660251 1110 26-27 -0.12276719358173693 1110 28-29 -0.10495442511602704 1110 30-31 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54-55 -0.10267417105117005 2115 56-57 0.06305354818154285 2115 58-59 -0.11565937725539754 2115 60-61 -0.07663558303699602 2115 62-63 -0.11663406263077292 2115 64-65 -0.05213285356487063 2115 66-67 0.16916841699558205 2115 68-69 0.06553067227616793 2115 70-71 0.23840846455593834 2115 72-73 0.2889102978587843 2115 74-75 0.6241569950252313 2115 76 0.699994024407502 2116 1 0.1081058997846398 2116 2 0.09068124614797313 2116 3 -0.003965781807622193 2116 4 0.005109048552945694 2116 5 0.13204057569647887 2116 6 0.16069329825879208 2116 7 0.049525915399122766 2116 8 0.06110794305267575 2116 9 0.20811916071748016 2116 10-11 0.05478072634132758 2116 12-13 0.10881011856911016 2116 14-15 0.1657492182672229 2116 16-17 -0.15851462892224788 2116 18-19 0.031850641588526685 2116 20-21 -0.04668891964829669 2116 22-23 0.04884149144926653 2116 24-25 -0.041064008719445155 2116 26-27 0.11885028945572174 2116 28-29 0.021689547372126583 2116 30-31 0.14893716940702717 2116 32-33 -0.1743532473140661 2116 34-35 -0.014683649889711603 2116 36-37 -0.1193963947183363 2116 38-39 0.12385113617242638 2116 40-41 -0.05183538259846898 2116 42-43 -0.09773509029403016 2116 44-45 -0.04214817631250156 2116 46-47 0.02464602209419553 2116 48-49 -0.11749080841253345 2116 50-51 0.12261475987880033 2116 52-53 0.044130235860947664 2116 54-55 0.12169658600100064 2116 56-57 0.14990990678262506 2116 58-59 0.12249100384403278 2116 60-61 0.08113467895353921 2116 62-63 0.25253609642615515 2116 64-65 0.1815693251964987 2116 66-67 0.16210398545868543 2116 68-69 0.1338896164714498 2116 70-71 0.1716486674821951 2116 72-73 -0.13131615607843372 2116 74-75 0.2810388419482521 2116 76 0.2381407293066502 2117 1 -0.05000489444230993 2117 2 0.039994183664177285 2117 3 -0.005694240886356283 2117 4 0.011547558621224141 2117 5 0.007894002366597874 2117 6 -0.042011740199420444 2117 7 0.12570774929420026 2117 8 -0.38816178298655757 2117 9 -0.0324823430419201 2117 10-11 -0.18309845436906613 2117 12-13 -0.26112333575636626 2117 14-15 -0.05132363628295167 2117 16-17 0.09276010964831727 2117 18-19 0.1433146464282089 2117 20-21 -0.04666731390981482 2117 22-23 0.02367080611524841 2117 24-25 -0.11093696697714961 2117 26-27 -0.100988099620416 2117 28-29 -0.7785221888650184 2117 30-31 -0.01234966837709095 2117 32-33 -0.20844710264204735 2117 34-35 0.03846646678127286 2117 36-37 0.058375621529179966 2117 38-39 -0.21531574655161734 2117 40-41 -0.29161586829547304 2117 42-43 -0.17905908994834618 2117 44-45 -0.1125180665553458 2117 46-47 -0.05782308169977313 2117 48-49 0.043947269540971945 2117 50-51 0.0655107930652079 2117 52-53 -0.04685152889577182 2117 54-55 0.11644639154935277 2117 56-57 -0.24257993852028648 2117 58-59 -0.08092702398415952 2117 60-61 -0.05042266267638951 2117 62-63 -0.12361981058273841 2117 64-65 -0.30723890226872186 2117 66-67 -0.13527739903703662 2117 68-69 -0.4393322312513064 2117 70-71 -0.1254302366747524 2117 72-73 -0.4048015938106957 2117 74-75 -0.15189694579697743 2117 76 -0.609193122564406 2118 1 -0.2160404212154461 2118 2 -0.16266575084362245 2118 3 -0.10321907486912352 2118 4 -0.03133336631886863 2118 5 0.07457530416123603 2118 6 0.05203725089144484 2118 7 0.09054685435612697 2118 8 0.2322212914879458 2118 9 -0.1801324903078978 2118 10-11 0.04246545540537028 2118 12-13 0.03269515206903861 2118 14-15 0.098165048933744 2118 16-17 0.042808588509700485 2118 18-19 -0.09862231275808142 2118 20-21 -0.07416306057574928 2118 22-23 -0.09984967391694965 2118 24-25 -0.005472576072889979 2118 26-27 -0.15084815120924588 2118 28-29 0.11516275341769244 2118 30-31 0.12278744447804257 2118 32-33 -0.12759716758542794 2118 34-35 0.09548858411841366 2118 36-37 0.1308103580054123 2118 38-39 0.19984624602105328 2118 40-41 0.2854472273999775 2118 42-43 0.14339851391533642 2118 44-45 0.3179101938767559 2118 46-47 -0.004889653238109304 2118 48-49 0.06705247781713553 2118 50-51 0.2145238366230089 2118 52-53 0.17586767788029078 2118 54-55 0.1739990322752547 2118 56-57 0.13444839874624392 2118 58-59 0.16153131163591183 2118 60-61 0.3226907778592576 2118 62-63 0.16770155853348 2118 64-65 0.033905930616413116 2118 66-67 -0.1327253985979766 2118 68-69 -0.04439415613651221 2118 70-71 0.0985421029333935 2118 72-73 0.40417339841439315 2118 74-75 -0.0025180240400288767 2118 76 -0.07349255728258441 2119 1 -0.017593357124432885 2119 2 -0.07100364990279218 2119 3 -0.033772912140761946 2119 4 0.003731408075367426 2119 5 -0.10395341278143633 2119 6 -0.023618298969225293 2119 7 -0.065235711051578 2119 8 0.1222668309210917 2119 9 0.002308022702948165 2119 10-11 0.14416037065961262 2119 12-13 0.009786990907755921 2119 14-15 -0.37514165590827986 2119 16-17 -0.01144107127007743 2119 18-19 0.0972885642736756 2119 20-21 0.14578415374236897 2119 22-23 0.140955543382141 2119 24-25 0.13055659744237147 2119 26-27 0.2222776919772258 2119 28-29 0.28872219327677584 2119 30-31 0.17400605143090075 2119 32-33 0.185461397421669 2119 34-35 0.07924638267270012 2119 36-37 0.10595219006917489 2119 38-39 0.1839663570123733 2119 40-41 0.2439815999514181 2119 42-43 0.30891933067950106 2119 44-45 0.2861268508228463 2119 46-47 0.22901689960942662 2119 48-49 0.270127125961686 2119 50-51 0.21289195784156334 2119 52-53 0.0714951853474659 2119 54-55 0.3180521007375816 2119 56-57 0.307545374762924 2119 58-59 0.395284691745843 2119 60-61 0.3813559363635832 2119 62-63 0.25758744484396345 2119 64-65 0.224025699914705 2119 66-67 0.4397194150320374 2119 68-69 0.3293267042229715 2119 70-71 0.47197429159508175 2119 72-73 0.46824967843772214 2119 74-75 0.03464992986675952 2119 76 -0.5752098593215536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 2.0 21 6.0 22 2.0 23 17.0 24 23.0 25 52.0 26 97.0 27 204.0 28 335.0 29 622.0 30 1015.0 31 1570.0 32 2484.0 33 4180.0 34 7126.0 35 12357.0 36 20217.0 37 106029.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.071114693070825 16.894696780713705 12.242626599888704 32.791561926326764 2 20.15364048637896 18.858378267738697 34.9528908333813 26.035090412501038 3 19.181394277819354 18.834072112524705 31.081815797721617 30.902717811934323 4 15.614146182334544 21.865305521974683 32.00736860284382 30.513179692846954 5 16.200692085787935 29.946462494962866 37.03362564683157 16.819219772417632 6 29.64135628346094 36.3421794945599 16.54225753011085 17.474206691868314 7 28.27957195581397 25.080754002520166 21.662541016636926 24.977133025028945 8 24.594630898240364 35.07378197378773 16.6490766859197 23.682510442052205 9 23.008334452695745 15.945477456041038 18.02237445551014 43.02381363575308 10-11 27.148696102699905 25.463256129308746 23.304485764908307 24.083562003083046 12-13 25.923473989215744 19.23576330921907 24.943552152693826 29.89721054887136 14-15 23.366530424270334 19.101439819878596 20.44243598846097 37.08959376739009 16-17 27.61435086574687 24.415213094621304 20.023154811019644 27.94728122861218 18-19 29.103102872603763 23.22868893878047 20.737627847178246 26.930580341437516 20-21 25.091307990968343 21.914237650234426 20.400859670331776 32.59359468846545 22-23 24.208930593134152 24.39378529989318 21.125247059275036 30.27203704769763 24-25 27.69238641669705 24.70624732152566 21.47736649204613 26.12399976973116 26-27 28.571565636213613 25.697042964327522 20.93943289902072 24.79195850043815 28-29 24.640364848182475 22.587454186095602 24.93651616039504 27.835664805326886 30-31 23.61023161207376 21.716910048036638 29.413645987245662 25.259212352643935 32-33 25.798105399164633 21.06320239991301 24.872552594042432 28.26613960687992 34-35 25.52402151734372 20.566525307185028 25.773159608287123 28.136293567184133 36-37 24.486532471104457 26.62835249042146 22.93509616922201 25.950018869252073 38-39 22.800452862049777 24.586635452446288 26.186684064756715 26.426227620747223 40-41 23.476227940565053 20.08743819520401 27.034840954592266 29.40149290963867 42-43 26.616519230646222 19.801521053607864 26.54647912549012 27.03548059025579 44-45 22.53884187566762 23.94348179277084 28.489372453450514 25.02830387811103 46-47 25.06060547911909 21.18057554417004 26.758838165780773 26.999980810930097 48-49 25.121690684985833 20.128374877669682 24.37587550131445 30.374058936030035 50-51 23.11035634102815 20.870672065191666 26.951048682670354 29.067922911109832 52-53 20.30587377429816 20.889541317265685 32.558094909139754 26.2464899992964 54-55 20.725474769571253 21.586424372677325 29.110458682734315 28.57764217501711 56-57 23.3754853235597 22.37829332412258 25.104100704238864 29.142120648078855 58-59 21.30306577373528 22.507819545986607 28.988927906664365 27.200186773613748 60-61 23.797644861486898 25.12584831679875 25.09386653362245 25.9826402880919 62-63 20.8530820844447 30.898560180121404 22.299618137508876 25.948739597925023 64-65 20.341373553623857 32.3837941908289 21.18249445116062 26.092337804386624 66-67 20.364400437510792 31.928373598398352 20.963099418571183 26.74412654551967 68-69 19.889151139510936 32.445519032359165 21.34080427788332 26.32452555024658 70-71 20.29595942151351 30.947492308381147 21.986196662381108 26.77035160772424 72-73 20.244148932767896 28.77816795553253 22.85770025393536 28.11998285776422 74-75 20.05417714070066 27.721489839387488 23.844977900587825 28.379355119324035 76 20.492007752384243 27.39239729050333 23.93196835082737 28.18362660628506 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 2.5 14 2.5 15 3.5 16 6.5 17 7.0 18 10.0 19 18.5 20 30.5 21 37.0 22 43.5 23 66.0 24 96.0 25 110.0 26 210.5 27 343.0 28 443.0 29 511.0 30 647.5 31 881.5 32 1259.5 33 1540.0 34 1728.0 35 2200.5 36 2691.0 37 2897.0 38 3450.5 39 6279.0 40 10035.5 41 11001.0 42 10485.0 43 9618.5 44 8477.5 45 7935.5 46 7668.0 47 7614.0 48 7214.0 49 6508.5 50 6149.0 51 5667.0 52 4562.5 53 3609.0 54 3278.0 55 3272.0 56 3003.5 57 2567.0 58 2393.0 59 2341.5 60 2297.0 61 2479.5 62 2655.0 63 2751.0 64 2872.0 65 2947.0 66 3150.0 67 3303.0 68 3338.0 69 3405.5 70 3340.0 71 3242.0 72 3259.5 73 3134.5 74 2771.5 75 2551.0 76 2261.0 77 1752.0 78 1283.0 79 1033.0 80 777.0 81 398.5 82 188.5 83 101.0 84 66.0 85 20.0 86 5.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 156339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.775634998304966 #Duplication Level Percentage of deduplicated Percentage of total 1 60.29308411214953 25.790749589034085 2 20.81943925233645 17.811294686546546 3 7.799626168224299 10.009018862855717 4 3.418317757009346 5.848828507282252 5 1.8362616822429907 3.927362974049981 6 1.274018691588785 3.2698175119451958 7 0.9420560747663551 2.820793276149905 8 0.6863551401869159 2.3487421564676763 9 0.577196261682243 2.222094295089517 >10 2.3177570093457946 14.961717805537965 >50 0.020934579439252334 0.5500866706324078 >100 0.007476635514018692 0.5027536315314797 >500 0.0014953271028037385 0.3268538240618144 >1k 0.004485981308411215 4.3188200001279275 >5k 0.0014953271028037385 5.291066208687532 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 8272 5.291066208687532 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 2480 1.5862964455446178 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 2140 1.368820319945759 No Hit CACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTTCTG 2132 1.3637032346375504 RNA PCR Primer, Index 15 (96% over 25bp) TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 511 0.3268538240618144 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 234 0.149674745265097 RNA PCR Primer, Index 48 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT 191 0.12217041173347662 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0057567209717345 0.0 2 0.0 0.0 0.0 0.009594534952890834 0.0 3 0.0 0.0 0.0 0.010234170616416889 0.0 4 0.0 0.0 0.0 0.04541413211034995 0.0 5 0.0 0.0 0.0 0.053729395736188665 0.0 6 0.0 0.0 0.0 0.05500866706324078 0.0 7 0.0 0.0 0.0 0.05500866706324078 0.0 8 0.0 0.0 0.0 0.056927574053818944 0.0 9 0.0 0.0 0.0 0.06204465936202739 0.0 10 0.0 0.0 0.0 0.06652210900670978 0.0 11 0.0 0.0 0.0 0.06652210900670978 0.0 12 0.0 0.0 0.0 0.06780138033376189 0.0 13 0.0 0.0 0.0 0.069080651660814 0.0 14 0.0 0.0 0.0 0.069080651660814 0.0 15 0.0 0.0 0.0 0.06972028732434006 0.0 16 0.0 0.0 0.0 0.07163919431491822 0.0 17 0.0 0.0 0.0 0.07291846564197034 0.0 18 0.0 0.0 0.0 0.0761166439596006 0.0 19 0.0 0.0 0.0 0.08315263625838722 0.0 20 0.0 0.0 0.0 0.0863508145760175 0.0 21 0.0 0.0 0.0 0.08699045023954356 0.0 22 0.0 0.0 0.0 0.0933868068748041 0.0 23 0.0 0.0 0.0 0.10298134182769494 0.0 24 0.0 0.0 0.0 0.10873806279942945 0.0 25 0.0 0.0 0.0 0.11769296208879422 0.0 26 0.0 0.0 0.0 0.12984603969578928 0.0 27 0.0 0.0 0.0 0.23154811019643212 0.0 28 0.0 0.0 0.0 0.30574584716545455 0.0 29 0.0 0.0 0.0 0.3997722897037847 0.0 30 0.0 0.0 0.0 0.48996091826095856 0.0 31 0.0 0.0 0.0 0.6146898726485394 0.0 32 0.0 0.0 0.0 0.7451755480078547 0.0 33 0.0 0.0 0.0 0.9287509834398326 0.0 34 0.0 0.0 0.0 1.1590198223092127 0.0 35 0.0 0.0 0.0 1.5178554295473299 0.0 36 0.0 0.0 0.0 1.9854290995848765 0.0 37 0.0 0.0 0.0 2.612911685503937 0.0 38 0.0 0.0 0.0 3.299240752467395 0.0 39 0.0 0.0 0.0 3.9753356488144354 0.0 40 0.0 0.0 0.0 4.686610506655409 0.0 41 0.0 0.0 0.0 5.561632094359053 0.0 42 0.0 0.0 0.0 6.423860968792176 0.0 43 0.0 0.0 0.0 7.332783246662701 0.0 44 0.0 0.0 0.0 8.165588880573626 0.0 45 0.0 0.0 0.0 9.031015933324378 0.0 46 0.0 0.0 0.0 9.89004662943987 0.0 47 0.0 0.0 0.0 10.734365705294264 0.0 48 0.0 0.0 0.0 11.497451051880848 0.0 49 0.0 0.0 0.0 12.185699025834884 0.0 50 0.0 0.0 0.0 12.91296477526401 0.0 51 0.0 0.0 0.0 13.584582221966368 0.0 52 0.0 0.0 0.0 14.327199227320119 0.0 53 0.0 0.0 0.0 15.081329674617338 0.0 54 0.0 0.0 0.0 15.839297935895713 0.0 55 0.0 0.0 0.0 16.530104452503853 0.0 56 0.0 0.0 0.0 17.147352867806497 0.0 57 0.0 0.0 0.0 17.799781244603075 0.0 58 0.0 0.0 0.0 18.393363140355255 0.0 59 0.0 0.0 0.0 18.965197423547547 0.0 60 0.0 0.0 0.0 19.518482272497586 0.0 61 0.0 0.0 0.0 20.18114481991058 0.0 62 0.0 0.0 0.0 20.777285258316862 0.0 63 0.0 0.0 0.0 21.40156966591829 0.0 64 0.0 0.0 0.0 22.027133344846774 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATGGAG 20 7.907238E-5 70.0 55 GTCAGTG 20 7.907238E-5 70.0 51 TTGCGAT 15 0.0022212998 70.0 49 ATGCCAT 15 0.0022212998 70.0 43 AACAGGA 15 0.0022212998 70.0 2 GCTATAC 25 2.3846285E-4 56.000004 3 CAGTGGT 25 2.3846285E-4 56.000004 53 TCCATAG 20 0.0069408608 52.5 28 TGCCATG 20 0.0069408608 52.5 19 CGTACTG 20 0.0069408608 52.5 5 GCTAGTC 20 0.0069408608 52.5 7 AGCTAGT 20 0.0069408608 52.5 6 CGGTAAG 20 0.0069408608 52.5 28 CAGAGAC 20 0.0069408608 52.5 7 GTCGGTT 20 0.0069408608 52.5 32 CTTTATA 75 0.0 51.33333 2 AAAGTCA 30 5.86374E-4 46.666664 43 GCATTAG 30 5.86374E-4 46.666664 34 CAAATTA 30 5.86374E-4 46.666664 24 GATTCAA 30 5.86374E-4 46.666664 20 >>END_MODULE