FastQCFastQC Report
Wed 25 May 2016
SRR1780233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735024
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGA508.393399E-526.3738732
ATATCTC609.61582E-625.7112421
CGACTAA703.195517E-522.01567537
CGGTATG500.002591282621.98271443
TCTGTAC703.2377902E-521.978231
AGTCTCT856.1244336E-620.69947410
CTCGCAA755.438972E-520.54796439
AAGTTGT654.876765E-420.34018119
CTTAATA654.962654E-420.2875962
ATGTTAA550.004437180220.0347321
CTGTACC901.0096775E-519.5362032
GCCGATA708.0803817E-418.87057938
CGTGATA708.106339E-418.86158224
TACTGAC708.1360905E-418.85130711
TACGGTT708.162199E-418.84232743
CCATATG951.6112757E-518.5054638
CGTTTAT600.00744014718.32017533
TATAGGA1203.1907803E-718.315192
GACTATC750.001276460217.62815915
ACGGTTG750.001297032317.58617244
Produced by FastQC (version 0.11.5)