FastQCFastQC Report
Wed 25 May 2016
SRR1780233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735024
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT244603.327782494177061No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC78551.0686725875617666No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCG63380.862284768932715No Hit
CACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGTCTTCTG52190.710044842073184RNA PCR Primer, Index 13 (96% over 25bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCC13040.17740917303380568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCT6500.036.2134843
CGTCTTC6800.035.2628542
TCTTCTG6850.034.36315544
CCGTCTT7400.031.8091441
ATGCCGT33600.031.42691844
TCTTTAT1500.029.397791
CGCAAAG300.005745041629.33179512
GCCGTCT8650.026.9581240
TATGCCG39900.026.46477743
TTGATAC609.755311E-625.66357228
GTATCGG551.592622E-424.00037214
ATATAAC551.5933572E-423.9987443
CTTTATA1850.023.7857692
AGACTAT651.8128747E-523.6910616
CTTATAT703.1591502E-522.0483441
ATTAGAG500.002578233422.0018373
TTAATAC803.6097354E-622.0018373
ACGTTAC500.002579252222.00034116
TATCGGC602.8709928E-422.00034115
AACCGTA602.8723144E-421.99884610
Produced by FastQC (version 0.11.5)