FastQCFastQC Report
Wed 25 May 2016
SRR1780232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177795
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGTC150.00222180370.052
CAAGGTT150.00222180370.06
TGCATGC200.006942420752.59
CTAGTGT200.006942420752.518
TATAAGA659.942596E-943.0769232
GCCCTAA400.00241395435.020
ATAAGAG851.0564145E-732.9411773
GTATAAG851.0564145E-732.9411771
AAGTATG450.004298764331.1111098
GGTCCAT450.004298764331.1111091
TTTTGCC450.004298764331.11110931
AGTGTCC450.004298764331.11110920
AGAGACA802.4531437E-630.6256
TGTTTCT500.007194239627.99999835
TAAGAGA1004.389276E-727.9999984
TCTCGTT657.9327513E-426.92307743
CAGGCAG657.9327513E-426.92307733
TAGATCT657.9327513E-426.92307739
AAGAGAC1001.3901321E-524.4999985
GTAGATC1151.4859706E-624.34782670
Produced by FastQC (version 0.11.5)